1994 年度実績報告書

粘菌細胞の分化と形態形成の制御機構

研究課題

研究課題/領域番号	05304057
研究機関	岡崎国立共同研究機構
研究代表者	竹内郁夫岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)
研究分担者	岡本浩二京都大学, 理学部, 教授 (10029944) 田仲可昌筑波大学, 生物科学系, 教授 (80091908) 柳澤嘉一郎筑波大学, 生物科学系, 教授 (60015899) 前田靖男東北大学, 理学部, 教授 (50025417) 落合廣北海道大学, 理学部, 教授 (10002122)
キーワード	細胞分化 / 形態形成 / 細胞接着 / 遺伝子発現 / 細胞周期 / 細胞性粘菌
研究概要	細胞性粘菌の発生系を対象として前年度に引き続き研究を行い、以下の成果を得た。形態形成の制御機構細胞接着にかかわる2種類の糖タンパク質(gp64とcsA)について、特徴あるアミノ酸配列(sushi領域)や糖鎖結合部位を明らかにし、接着における機能との関連を考察した(落合・吉田元信)。有性的細胞融合の分子機構を明らかにするため、細胞融合能を獲得した細胞のみで発現する遺伝子を単離した(柳澤)。粘菌細胞に葉酸による走化性刺激を局所的に与えると、ミオシンの構築が速やかに変化することを見い出した(祐村恵彦)。細胞分化の制御機構粘菌で発現する遺伝子にβ-galを連結させたDNAによる形質転換株を用い、飢餓処理時における細胞周期上の位置と集合体形成の順序ならびに細胞分化が密接に関連することを明らかにした(竹内・前田)。細胞周期内の増殖/分化の分岐点前後で特異的に発現する3種の遺伝子を発見した。またその時点でリン酸化レベルが著しく変動する4種のタンパク質も見い出し、増殖/分化の制御におけるリン酸化レベルの重要性を示した(前田)。ある種の予定柄特異的遺伝子は細胞から分泌される物質および8-Br-cAMPによって発現が誘導されることを見い出し、これら物質の遺伝子上における作用点を決定した(岡本)。予定胞子分化誘導因子の精製を進め、95Kdの糖タンパク質が2量体を形成していることを明らかにし、またこの因子は細胞分裂誘導能をももつことを見い出した(大畠明子)。多くの粘菌種に形質転換が行えるように、Bsr-REMI法の改良を試みた(田仲)。種々の条件下で細胞内イノシトール3リン酸とcGMPの定量を行い、従来の考えとは異なり両者は独立に変動することを明らかにした(小山正和)。

研究成果

(24件)

すべてその他

すべて文献書誌 (24件)

[文献書誌] Takeuchi I.: "Regulation of cell differentiation and pattern formation in Dictyostelium development" Int.J.Dev.Biol.38. 311-319 (1994)
[文献書誌] Morio T.: "Cooperation of pasitively and negatively acting promoter elements determines prespore-specific transcription of Dp87 gene in Dictyostelium" Mech.Dev.45. 59-72 (1994)
[文献書誌] Nakao H.: "Dictyostelium prespore-specific gene Dp87 encodes a sorus matrix protein" J.Cell Sci.107. 397-403 (1994)
[文献書誌] Saran S.: "Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA" FEBS Lett.377. 43-47 (1994)
[文献書誌] Traynor D.: "Aberrant pattern formation in myosin heavy chain mutants of Dictyostelium" Development. 120. 591-601 (1994)
[文献書誌] Araki T.: "Cell-cycle-dependent sorting in the development of Dictyostelium cells" Dev.Biol.162. 221-228 (1994)
[文献書誌] Manabe R.: "Molecular cloning and the COOH-terminal processing of Gp64,a putative cell-cell adhesion protein of the cellular slime mold Polysphondylium pallidum" J.Biol.Chem.269. 528-535 (1994)
[文献書誌] Saito T.: "Assignment of disulfide bonds in gp64,a putative cell-cell adhesion protein of Polysphondylium pallidum-Presence of sushi domains in the cellular slime mold protein" J.Biol.Chem.269. 28798-28802 (1994)
[文献書誌] Ochiai H.: "Effects of tunicamycin on the development of Dictyostelium discoideum" Animal Biology. (in press). (1995)
[文献書誌] 吉田元信: "生物界におけるシアル酸の分布と機能" 遺伝. 48. 49-54 (1994)
[文献書誌] Yoshida M.: "Characterization and distribution of O-glycosylated carbohydrates in the cell adhesion molecule,contact site A,from Dictyostelium discoideum" Exp.Cell Res.217(in press). (1995)
[文献書誌] Morio T.: "Bsr-REMI:an improved method for gene tagging using a new vector in Dictyostelium" J.Plant Res.(in press). (1994)
[文献書誌] Yumura S.: "Rapid translocation of myosin II in vegetative Dictyostelium amoeba during folate-stimulation" Cell Struct.Funct.19. 143-151 (1994)
[文献書誌] Okazaki K.: "Differential association of three actin-bundling proteins with microfilaments in Dictyostelium amoebae" Eur.J.Cell Biol.66. 75-81 (1995)
[文献書誌] Furukawa T.: "K252a,a potent inhibitor of protein kinases,promotes the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.11. 69-76 (1994)
[文献書誌] 荒木剛: "粘菌の形態形成" 遺伝. 別冊 6. 8-17 (1994)
[文献書誌] Abe F.: "Precise expression of the cyclic AMP receptor 1(CAR1)gene during transition from growth to differentiation in Dictyostelium discoideum" FEBS Lett.342. 239-241 (1994)
[文献書誌] Iijima N.: "A proteinous factor mediation intercellular communication during the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.12. 61-69 (1995)
[文献書誌] Abe F.: "Specific expression of a gene encoding a novel caicium-binding protein,CAF-1,during transition of Dictyostelium cells from growth to differentiation" Dev.Growth & Differ.37(in press). (1995)
[文献書誌] Yoshida H.: "dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum" Nuc.Acids Res.22. 41-46 (1994)
[文献書誌] Yamada Y.: "Analysis of cellular interactions involved in differential control of prestalk genes in Dictyostelium discoideum" Dev.Biol.161. 296-301 (1994)
[文献書誌] Kubohara Y.: "Cytoplasmic Ca^<2+> and H^+ concentrations determine cell fate in Dictyostelium discoideum" FASEB J.8. 869-874 (1994)
[文献書誌] Kubohara Y.: "Specific induction by zine of Dictyostelium stalk cell differentiation" Exp.Cell Res.214. 367-372 (1994)
[文献書誌] Agarwal A.: "Analysis of a novel cyclic AMP inducible prespore gene in Dictyostelium discoideum:Evidence for different patterns of cAMP regulation" Differentiation. 57. 151-162 (1994)

1994 年度 実績報告書

粘菌細胞の分化と形態形成の制御機構

研究代表者

竹内 郁夫 岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)

研究成果

[文献書誌] Takeuchi I.: "Regulation of cell differentiation and pattern formation in Dictyostelium development" Int.J.Dev.Biol.38. 311-319 (1994)

[文献書誌] Morio T.: "Cooperation of pasitively and negatively acting promoter elements determines prespore-specific transcription of Dp87 gene in Dictyostelium" Mech.Dev.45. 59-72 (1994)

[文献書誌] Nakao H.: "Dictyostelium prespore-specific gene Dp87 encodes a sorus matrix protein" J.Cell Sci.107. 397-403 (1994)

[文献書誌] Saran S.: "Intracellular free calcium level and its response to cAMP stimulation in developing Dictyostelium cells transformed with jellyfish apoaequorin cDNA" FEBS Lett.377. 43-47 (1994)

[文献書誌] Traynor D.: "Aberrant pattern formation in myosin heavy chain mutants of Dictyostelium" Development. 120. 591-601 (1994)

[文献書誌] Araki T.: "Cell-cycle-dependent sorting in the development of Dictyostelium cells" Dev.Biol.162. 221-228 (1994)

[文献書誌] Manabe R.: "Molecular cloning and the COOH-terminal processing of Gp64,a putative cell-cell adhesion protein of the cellular slime mold Polysphondylium pallidum" J.Biol.Chem.269. 528-535 (1994)

[文献書誌] Saito T.: "Assignment of disulfide bonds in gp64,a putative cell-cell adhesion protein of Polysphondylium pallidum-Presence of sushi domains in the cellular slime mold protein" J.Biol.Chem.269. 28798-28802 (1994)

[文献書誌] Ochiai H.: "Effects of tunicamycin on the development of Dictyostelium discoideum" Animal Biology. (in press). (1995)

[文献書誌] 吉田元信: "生物界におけるシアル酸の分布と機能" 遺伝. 48. 49-54 (1994)

[文献書誌] Yoshida M.: "Characterization and distribution of O-glycosylated carbohydrates in the cell adhesion molecule,contact site A,from Dictyostelium discoideum" Exp.Cell Res.217(in press). (1995)

[文献書誌] Morio T.: "Bsr-REMI:an improved method for gene tagging using a new vector in Dictyostelium" J.Plant Res.(in press). (1994)

[文献書誌] Yumura S.: "Rapid translocation of myosin II in vegetative Dictyostelium amoeba during folate-stimulation" Cell Struct.Funct.19. 143-151 (1994)

[文献書誌] Okazaki K.: "Differential association of three actin-bundling proteins with microfilaments in Dictyostelium amoebae" Eur.J.Cell Biol.66. 75-81 (1995)

[文献書誌] Furukawa T.: "K252a,a potent inhibitor of protein kinases,promotes the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.11. 69-76 (1994)

[文献書誌] 荒木 剛: "粘菌の形態形成" 遺伝. 別冊 6. 8-17 (1994)

[文献書誌] Abe F.: "Precise expression of the cyclic AMP receptor 1(CAR1)gene during transition from growth to differentiation in Dictyostelium discoideum" FEBS Lett.342. 239-241 (1994)

[文献書誌] Iijima N.: "A proteinous factor mediation intercellular communication during the transition of Dictyostelium cells from growth to differentiation" Zool.Sci.12. 61-69 (1995)

[文献書誌] Abe F.: "Specific expression of a gene encoding a novel caicium-binding protein,CAF-1,during transition of Dictyostelium cells from growth to differentiation" Dev.Growth & Differ.37(in press). (1995)

[文献書誌] Yoshida H.: "dutA RNA functions as an untranslatable RNA in the development of Dictyostelium discoideum" Nuc.Acids Res.22. 41-46 (1994)

[文献書誌] Yamada Y.: "Analysis of cellular interactions involved in differential control of prestalk genes in Dictyostelium discoideum" Dev.Biol.161. 296-301 (1994)

[文献書誌] Kubohara Y.: "Cytoplasmic Ca^<2+> and H^+ concentrations determine cell fate in Dictyostelium discoideum" FASEB J.8. 869-874 (1994)

[文献書誌] Kubohara Y.: "Specific induction by zine of Dictyostelium stalk cell differentiation" Exp.Cell Res.214. 367-372 (1994)

[文献書誌] Agarwal A.: "Analysis of a novel cyclic AMP inducible prespore gene in Dictyostelium discoideum:Evidence for different patterns of cAMP regulation" Differentiation. 57. 151-162 (1994)

1994 年度実績報告書

竹内郁夫岡崎国立共同研究機構, 基礎生物学研究所, 所長 (90025239)

[文献書誌] 荒木剛: "粘菌の形態形成" 遺伝. 別冊 6. 8-17 (1994)