1995 年度実績報告書

植物器官の代謝発現に関わる遺伝的プログラム

研究課題

研究課題/領域番号	06278102
研究機関	名古屋大学
研究代表者	中村研三名古屋大学, 農学部, 教授 (80164292)
研究分担者	杉山達夫名古屋大学, 農学部, 教授 (50023453) 篠崎一雄理化学研究所, ライフサイエンス筑波研究センター, 主任研究員 (20124216) 島本功奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10263427) 大山莞爾京都大学, 農学部, 教授 (40135546) 内宮博文東京大学, 分子細胞生物学研究所, 教授 (50142229)
キーワード	植物器官 / 遺伝子発現制御 / 制御遺伝子 / 転写制御因子 / 情報伝達因子 / 遺伝的プログラム / モデル植物
研究概要	2種の糖応答性遺伝子に共通した糖応答性エレメントに結合しうるタンパク質のcDNAが単離され、その発現自体が糖によって誘導されることが示された(中村)。種子ABA応答性遺伝子のACGTGモチーフに結合するbZIP因子のmRNAがタンパク質合成阻害下でもABAによって短時間のうちに誘導されることが明らかになり、同じくABA応答性遺伝子の転写制御因子であるVp1とin vivoで相互作用するタンパク質が検出された(服部)。シロイヌナズナから乾燥やABAによって誘導され、これら刺激応答性エレメントに結合するMYB因子cDNAが単離され、その結合活性はリン酸化によって失われること(篠崎)、ヒストン遺伝子の分裂組織での転写に関与するbZIP因子の一つが核内のCDPKと思われる活性によってリン酸化されることでDNA結合活性が調節されること(飯)が明らかになった。公募班員(杉田)によってタバコの核抽出液からRNA pol.IIによる忠実な転写開始を起こすin vitro転写系が開発された。糖応答性遺伝子発現にはCa2+-情報伝達系とタンパク質リン酸化が関与するが、タバコ葉の膜結合型カルシウム依存性プロテインキナーゼ(CDPK)が糖による誘導を受けることが明らかになり(中村)、窒素同化に関わる遺伝子の硝酸シグナルによる誘導にもリン酸化・脱リン酸化反応やCa2+情報伝達系が関与することが示唆された(杉山)。シロイヌナズナから単離された糖応答性遺伝子発現に関わる変異株lbalは葉の正常な成長にも異常が見られ、この表現型から新たな糖応答性変異株がT-DNAタツギングライブラリーから同定された(中村)。トウモロコシのAcとCsを組み合わせたイネの高頻度変異誘発系が開発され、開花時期や胚発生、病原菌抵抗性などに関する変異株が分離されて、原因遺伝子の単離へ向けた研究が順調に進んでいる(島本)。シロイヌナズナの遊離メチオニンを過剰蓄積するmto1-1変異株における変異の原因究明、およびそのもたらす生長生理への影響の解析が進んだ(内藤)。

研究成果
(29件)

すべてその他

すべて文献書誌 (29件)

[文献書誌] S.Ohto: "lnvolvement of Ca^<2+>-signalling in the sugar-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant Journal. 7. 297-307 (1995)
[文献書誌] S.Mita: "Sugar-inducible expression of a gene for β-amylase in Arabidopsis thaliana." Plant Physiology. 107. 895-904 (1995)
[文献書誌] M.Suzuki: "Differential regulation of the expression in transgenic tobacco of the gene for β-glucuronidase under the control of the 5′-upstream regions of two catalase genes from castor bean." Plant & Cell Physiology. 36. 273-279 (1995)
[文献書誌] S.Takeda: "Spatial patterns of sucrose-inducible and polygalacturonic acidinducible expression of genes that encode sporamin and β-amylase in sweet potato." Plant & Cell Physiology. 36. 321-333 (1995)
[文献書誌] M.Suzuki: "Different sets of cis-elements contribute to the expression of a catalase gene from castor bean during seed formation and postembryonic development in transgenic tobacco." Plant & Cell Physiology. 36. 1067-1074 (1995)
[文献書誌] K.Matsuoka: "Different sensitivity to wortmannin of two vacuolar sorting signals indicates the presence of distinct sorting machineries in tobacco cells." Journal of Cell Biology. 130. 1307-1318 (1995)
[文献書誌] M.Ohto: "Sugar-induced increases of calcium-dependent protein kinases (CDPK′s) associated with the plasma membrane in leaf tissues of tobacco." Plant Physiology. 109. 973-981 (1995)
[文献書誌] K.Matsuoka: "O-glycosylation of a precursor to a sweet potato vacuolar protein,sporamin,expressed in tobacco cells." Plant Journal. 877-889 (1995)
[文献書誌] M.Takemura: "Active transcription of the pseudogene for the subunit 7 of the NADH dehydrogenase in Marchantia polymorpha." Molecular and General Genetics. 247. 565-570 (1995)
[文献書誌] K.ltoh: "Developmental and hormonal regulation of rice a-amylase (RAmy1A) -gusA fusion genes in transgenic rice." Plant Physiology. 107. 25-31 (1995)
[文献書誌] R.Terada: "A type l element composed of the hexamer (ACGTCA) and octamer (OGCGATC) motifs plays a role (s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants." Plant Molecular Biology. 27. 17-26 (1995)
[文献書誌] A.Yano: "Expression of functional proteins of cDNA encodiog rice nucleoside diphosphate kinase (NDK) in Escherichia coliand organ related alteration of NDK activities during rice seed germination." Plant Molecular Biology. 27. 1053-1058 (1995)
[文献書誌] M.Kawai: "Tissue specific localization of adenylate kinase in rice (Oryza sativaL.) plants." Journal of Plant Physiology. 146. 239-243 (1995)
[文献書誌] M.Kawai: "Biochemical properties of rice adenylate kinase and subcellular location in plant cells." Plant Molecular Biology. 27. 943-951 (1995)
[文献書誌] J.Liu: "Analysis of randomly isolated cDNAs from developing endosperm of rice (Oryza sativa L.) : Evaluation of expressed sequence tags,and expression levels of mRNAs." Plant Molecular Biology. 685-689 (1995)
[文献書誌] T.Anai: "lmmunological analysis of small GTP-binding protein in higher plant cells." Journal of Plant Physiology. (in press). (1995)
[文献書誌] T.Mizoguchi: "Two genes that encode ribosomal-protein S6 kinase homologs are induced by cold or salinity stress in Arabidopsis thaliana." FEBS Letters. 358. 199-244 (1995)
[文献書誌] T.Iwasaki: "Identification of a cis-reguratory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis." Molecular and General Genetics. 247. 391-398 (1995)
[文献書誌] T.Hirayama: "AtPLC1,a gene encoding phospholipase C,is induced by drought and salt stress in Arabidopsis thaliana." Proc.Natl.Acad.Sci.USA. 92. 3903-3907 (1995)
[文献書誌] H.Sakakibara: "Isolation and characterization of a cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)
[文献書誌] M.Taniguchi: "Aspartate aminotransferase isozymes in Panicum miliaceumL.,an NAD-malic enzyme-type C4 plant:comparison of enzymatic properties,primary structures,and expression patterns." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)
[文献書誌] S.Naito: "Expression of soybean seed storage protein genes in transgenic plants and their response to sulfur nutritional conditions." Journal of Plant Physiology. 145. 614-619 (1995)
[文献書誌] E.Nambara: "A regulatory role for the AB13 gene in the establishment of embryo maturation in Arabidopsis thaliana." Development. 121. 629-636 (1995)
[文献書誌] M.Hirai: "Effects of sulfate concentrations on the expression of a soybean seed storage protein gene and its reversibility in transgenic Arabidopsis thaliana." Plant and Cell Physiology. (in press). (1995)
[文献書誌] T.Hattori: "Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1:analysis of cis-actiog promoter elements required for regulation by abcisic acid and VP1." Plant Journal. 7. 913-925 (1995)
[文献書誌] G.H.Huh: "Differential expression of the two types of histone H2A genes in wheat." Biochimistry,Biophysics Acta. 1261. 155-160 (1995)
[文献書誌] H.Takase: "Characteristics of the cDNA encoding a wheat protein with RNA recognition motif 1." Plant Physiology. (in press). (1995)
[文献書誌] T.Meshi: "Plant transcription factors." Plant and Cell Physiology. (in press). (1995)
[文献書誌] K.Ohyama: "The mitochondrial genome of a liverwort,Marchantia polymorpha.ln “Advances in Cellular and Molecular Biology of Plants",eds,Levings,C.S.lll and Vasil,l.K.Kluwer Academic Publishers," 598-634 (1995)

1995 年度 実績報告書

植物器官の代謝発現に関わる遺伝的プログラム

研究代表者

中村 研三 名古屋大学, 農学部, 教授 (80164292)

研究成果

[文献書誌] S.Ohto: "lnvolvement of Ca^<2+>-signalling in the sugar-inducible expression of genes coding for sporamin and β-amylase of sweet potato." Plant Journal. 7. 297-307 (1995)

[文献書誌] S.Mita: "Sugar-inducible expression of a gene for β-amylase in Arabidopsis thaliana." Plant Physiology. 107. 895-904 (1995)

[文献書誌] M.Suzuki: "Differential regulation of the expression in transgenic tobacco of the gene for β-glucuronidase under the control of the 5′-upstream regions of two catalase genes from castor bean." Plant & Cell Physiology. 36. 273-279 (1995)

[文献書誌] S.Takeda: "Spatial patterns of sucrose-inducible and polygalacturonic acidinducible expression of genes that encode sporamin and β-amylase in sweet potato." Plant & Cell Physiology. 36. 321-333 (1995)

[文献書誌] M.Suzuki: "Different sets of cis-elements contribute to the expression of a catalase gene from castor bean during seed formation and postembryonic development in transgenic tobacco." Plant & Cell Physiology. 36. 1067-1074 (1995)

[文献書誌] K.Matsuoka: "Different sensitivity to wortmannin of two vacuolar sorting signals indicates the presence of distinct sorting machineries in tobacco cells." Journal of Cell Biology. 130. 1307-1318 (1995)

[文献書誌] M.Ohto: "Sugar-induced increases of calcium-dependent protein kinases (CDPK′s) associated with the plasma membrane in leaf tissues of tobacco." Plant Physiology. 109. 973-981 (1995)

[文献書誌] K.Matsuoka: "O-glycosylation of a precursor to a sweet potato vacuolar protein,sporamin,expressed in tobacco cells." Plant Journal. 877-889 (1995)

[文献書誌] M.Takemura: "Active transcription of the pseudogene for the subunit 7 of the NADH dehydrogenase in Marchantia polymorpha." Molecular and General Genetics. 247. 565-570 (1995)

[文献書誌] K.ltoh: "Developmental and hormonal regulation of rice a-amylase (RAmy1A) -gusA fusion genes in transgenic rice." Plant Physiology. 107. 25-31 (1995)

[文献書誌] R.Terada: "A type l element composed of the hexamer (ACGTCA) and octamer (OGCGATC) motifs plays a role (s) in meristematic expression of a wheat histone H3 gene in transgenic rice plants." Plant Molecular Biology. 27. 17-26 (1995)

[文献書誌] A.Yano: "Expression of functional proteins of cDNA encodiog rice nucleoside diphosphate kinase (NDK) in Escherichia coliand organ related alteration of NDK activities during rice seed germination." Plant Molecular Biology. 27. 1053-1058 (1995)

[文献書誌] M.Kawai: "Tissue specific localization of adenylate kinase in rice (Oryza sativaL.) plants." Journal of Plant Physiology. 146. 239-243 (1995)

[文献書誌] M.Kawai: "Biochemical properties of rice adenylate kinase and subcellular location in plant cells." Plant Molecular Biology. 27. 943-951 (1995)

[文献書誌] J.Liu: "Analysis of randomly isolated cDNAs from developing endosperm of rice (Oryza sativa L.) : Evaluation of expressed sequence tags,and expression levels of mRNAs." Plant Molecular Biology. 685-689 (1995)

[文献書誌] T.Anai: "lmmunological analysis of small GTP-binding protein in higher plant cells." Journal of Plant Physiology. (in press). (1995)

[文献書誌] T.Mizoguchi: "Two genes that encode ribosomal-protein S6 kinase homologs are induced by cold or salinity stress in Arabidopsis thaliana." FEBS Letters. 358. 199-244 (1995)

[文献書誌] T.Iwasaki: "Identification of a cis-reguratory region of a gene in Arabidopsis thaliana whose induction by dehydration is mediated by abscisic acid and requires protein synthesis." Molecular and General Genetics. 247. 391-398 (1995)

[文献書誌] T.Hirayama: "AtPLC1,a gene encoding phospholipase C,is induced by drought and salt stress in Arabidopsis thaliana." Proc.Natl.Acad.Sci.USA. 92. 3903-3907 (1995)

[文献書誌] H.Sakakibara: "Isolation and characterization of a cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)

[文献書誌] M.Taniguchi: "Aspartate aminotransferase isozymes in Panicum miliaceumL.,an NAD-malic enzyme-type C4 plant:comparison of enzymatic properties,primary structures,and expression patterns." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)

[文献書誌] S.Naito: "Expression of soybean seed storage protein genes in transgenic plants and their response to sulfur nutritional conditions." Journal of Plant Physiology. 145. 614-619 (1995)

[文献書誌] E.Nambara: "A regulatory role for the AB13 gene in the establishment of embryo maturation in Arabidopsis thaliana." Development. 121. 629-636 (1995)

[文献書誌] M.Hirai: "Effects of sulfate concentrations on the expression of a soybean seed storage protein gene and its reversibility in transgenic Arabidopsis thaliana." Plant and Cell Physiology. (in press). (1995)

[文献書誌] T.Hattori: "Regulation of the Osem gene by abscisic acid and the transcriptional activator VP1:analysis of cis-actiog promoter elements required for regulation by abcisic acid and VP1." Plant Journal. 7. 913-925 (1995)

[文献書誌] G.H.Huh: "Differential expression of the two types of histone H2A genes in wheat." Biochimistry,Biophysics Acta. 1261. 155-160 (1995)

[文献書誌] H.Takase: "Characteristics of the cDNA encoding a wheat protein with RNA recognition motif 1." Plant Physiology. (in press). (1995)

[文献書誌] T.Meshi: "Plant transcription factors." Plant and Cell Physiology. (in press). (1995)

[文献書誌] K.Ohyama: "The mitochondrial genome of a liverwort,Marchantia polymorpha.ln “Advances in Cellular and Molecular Biology of Plants",eds,Levings,C.S.lll and Vasil,l.K.Kluwer Academic Publishers," 598-634 (1995)

1995 年度実績報告書

中村研三名古屋大学, 農学部, 教授 (80164292)