1996 年度実績報告書

低分子量G蛋白質による細胞機能の制御機構

研究課題

研究課題/領域番号	06404021
研究機関	大阪大学
研究代表者	高井義美大阪大学, 医学部, 教授 (60093514)
キーワード	低分子量G蛋白質 / アクチン細胞骨格 / 神経伝達物質 / BNI1 / BNR1 / プロフィリン / PKC1 / Rabphilin-3A / Doc2
研究概要	本年度の本研究では、低分子量G蛋白質のうち、主にRhoおよびRab3Aの機能、活性化機構および作用機構について検討した。私共は、Rhoは、アクチン繊維と細胞膜との結合を制御するERM-CD44系を介してアクチン細胞骨格系の再編成を制御していることを明らかにしているが、本年度の本研究で、Rhoは、何らかの標的蛋白質を介して、ERMが細胞膜のCD44に結合して細胞質から細胞膜に移行する過程に作用することを明らかにした。さらに、このERMが、Rho-Rho GDI複合体に直接作用し、RhoをRho GDIから解離させて活性化反応を開始させる作用も有していることを明らかにした。また、酵母の遺伝学的手法を用いて、新たにRhoの標的蛋白質がBNI1とBNR1であることを明らかにすると共に、BNI1とBNR1が、アクチン結合蛋白質であるプロフィリンに直接結合することを示した。したがって、RhoはBNI1とBNR1を介してアクチン細胞骨格系に作用する可能性が出てきた。さらに、Racが、何らかの標的蛋白質を介して、E-カドヘリン系を制御することにより、細胞間接着を制御していることを明らかにした。一方、Rab3Aは神経伝達物質の放出を制御しており、私共は、Rab3Aの標的蛋白質としてRabphilin3Aを見い出している。本年度の本研究において、私共は、Rabphilin-3Aが実際に神経伝達物質の放出に働いていることを証明した。また、Rabphilin-3Aがアクチン結合蛋白質であるα-アクチニンに結合し、α-アクチニンによるF-アクチンのbundingを促進することを明らかにした。その結果、Rabphilin-3Aが細胞骨格系をも介して神経伝達物質の放出を制御している可能性が明らかになった。さらに、私共は、世界に先駆けてRab3AのGAPおよびGEPを精製すると共に、そのcDNAをクローニングして一次構造を決定した。私共は、Rabphilin-3Aと同様C2様ドメインを2個有する蛋白質としてDoc2を見い出しているが、本年度の本研究で、このDoc2も実際に神経伝達物質の放出に働いていることを証明した。さらに、新たにDoc2に結合する蛋白質としてホルボールエステル結合領域であるC1ドメインを有する新しい蛋白質を見い出した。以上の結果を得たことにより、本年度の研究計画はほぼ達成できたと考えている。

研究成果

(68件)

すべてその他

すべて文献書誌 (68件)

[文献書誌] Miki,H.: "Association of Ash/Grb-2 with dynamin through the Src homology 3 domain." J.Biol.Chem.269. 5489-5492 (1994)
[文献書誌] Nishimura,N.: "Molecular cloning and characterization of two rab GDI species from rat brain-Brain-specific and ubiquitous types." J.Biol.Chem.269. 14191-14198 (1994)
[文献書誌] Nakanishi,H.: "Different functions of smg GDP dissociation stimulator and mammalian counterpart of yeast Cdc25." J.Biol.Chem.269. 15085-15091 (1994)
[文献書誌] Masuda,T.: "Molecular cloning and characterization of yeast rho GDP dissociation inhibitor." J.Biol.Chem.269. 19173-19718 (1994)
[文献書誌] Shimizu,K.: "Synergistic activation by Ras and 14-3-3 protein of a mitogen-activated protein kinase kinase kinase named Ras-dependent extracellular signal-regulated kinase kinase stimulator." J.Biol.Chem.269. 22917-22920 (1994)
[文献書誌] Shirataki,H.: "Rabphilin-3A is associated with synaptic vesicle through a vesicle protein in a manner independent of Rab3A." J.Biol.Chem.269. 32717-32720 (1994)
[文献書誌] Nishiyama,T.: "Rac p21 is involved in insulin-induced membrane ruffling and rho p21 is involved in hepatocyte growth factor and 12-O-tetradecanoylphorbol-13-acetate(TPA)-induced membrane ruffling in KB cells." Mol.Cell.Biol.14. 2447-2456 (1994)
[文献書誌] Yamochi,W.: "Growth site localization of Rhol small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae." J.Cell.Biol.125. 1077-1093 (1994)
[文献書誌] Huber,L.A.: "Mapping of ras-ralated GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis." Proc.Natl.Acad.Sci.USA. 91. 7874-7878 (1994)
[文献書誌] Takaishi,K.: "Involvement of Rho p21 small GTP-binding protein and its regulator in the HGF-induced cell motility." Oncogene. 9. 273-279 (1994)
[文献書誌] Wada,K.: "Localization of Rabphilin-3A,a putative target for Rab3A,at the aites of Ca^<2+>-dependent cxocytosis in Pc12 cells." Biochem.Biophys.Res.Commun.198. 158-165 (1994)
[文献書誌] Yaku,H.: "The Db1 oncogene product as a GDP/GTP exchange protein for the family-Its properties in comparison with those of Smg GDS." Biochem.Biophys.Res.Commun.198. 811-818 (1994)
[文献書誌] Nakanishi,H.: "Kinetic proerties of Ash/Grb2-interacting GDP/GTP exchange protein." Biochem.Biophys.Res.Commun.198. 1255-1261 (1994)
[文献書誌] Kim.Y.S.: "Phosphoprylation and activation of mitogen-activated protein kinase by kainic acid-induced seizure in rat hippocampus." Biochem.Biophys.Res.Commun.202. 1163-1168 (1994)
[文献書誌] Mizoguchi,A.: "Localization of Rabphilin-3A on the synaptic vesicle." Biochem.Biophys.Res.Commun.202. 1235-1243 (1994)
[文献書誌] Numata,S.: "Phosphorylation of Rabphilin-3A,a putative target protein for Rab3A,by cyclic AMP-dependent protein kinase." Biochem.Biophys.Res.Commun.203. 1927-1934 (1994)
[文献書誌] Miyazaki,M.: "Identification as β-adducin of a protein interacting with Rabphilin-3A in the presence of Ca^<2+> and phosphatidylserine." Biochem.Biophys.Res.Commun.205. 460-466 (1994)
[文献書誌] Imazumi,K.: "Identification of a Rabphilin-3A-interacting protein as GTP cyclohydorase I in PC12 cells." Biochem.Biophys.Res.Commun.205. 1409-1416 (1994)
[文献書誌] Kato.M.: "Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinese II." Biochem.Biophys.Res.Commun.205. 1776-1784 (1994)
[文献書誌] Miyazaki,A.: "Comparison of kinetic properties between MSS4 and Pab3A GRF GDP/GTP exchange proteins." FEBS Lett.350. 333-336 (1994)
[文献書誌] Fujita,Y.: "GDP/GTP exchange reaction-atimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein." FEBS Lett.353. 67-70 (1994)
[文献書誌] Kuroda,S.: "Purification and characterization of REKS from Xenopus eggs-Identification of REKS as a Rasdependent mitogen-activated protein kinase kinese kinese" J.Biol.Chem.270. 2460-2465 (1995)
[文献書誌] Bourgoin,S.: "Low molecular weight GTP-binding proteins in HL-60 granulocytes." J.Biol.Chem.270. 3172-3178 (1995)
[文献書誌] Yamamori,B.: "Purification of a Ras-dependent mitogen-activated protein kinase kinase kinase from bovine brain cytosol and its identification as a complex of B-raf and 14-3-3 proteins." J.Biol.Chem.270. 11723-11726 (1995)
[文献書誌] Chung,S-H.: "Evidence that the Rab3A-binding protein,Rabphilin-3A,enhances regulated secretion-Studies in adrenal chromaffin cells." J.Biol.Chem.270. 16714-16718 (1995)
[文献書誌] Kuribara,H.: "Synergistic activation of rat brain phosholipase D by ADP-ribosylation factor and rhoA p21,and its inhibition by clostridium botulinum C3 exoenzyme." J.Biol.Chem.270. 25667-25671 (1995)
[文献書誌] McGeady,P.: "The farnesyl group of H-ras facilitates the activation of a soluble upstream activator of mitogen-activated protein kinase." J.Biol.Chem.270. 26347-26351 (1995)
[文献書誌] Gotoh,T.: "Identification of Rapl as a target for the Crk SH3 Domain-binding guanine nucleotide-releasing factor C3G." Mol.Cell.Biol.15. 6746-6753 (1995)
[文献書誌] Nonaka,H.: "A downstream target of RHO1 small GTP-binding protein is PKC1,a homolog of protein kinase C,which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae." EMBO.J.14. 5931-5938 (1995)
[文献書誌] Takaishi,K.: "Translocation of activated Rho from the cytoplasm to membrane ruffling area,cell-cell adhesion sites and cleavage furrows." Oncogene. 11. 39-48 (1995)
[文献書誌] Nishimura,N.: "Cloning of a brain-type isoform of human rab GDI and its expression in human neuroblastoma cell lines and tumor specimens." Cancer Res.55. 5445-5450 (1995)
[文献書誌] Orita,S.: "Doc2: a novel brain protein having two repeated C_2-like domains." Biochem.Biophys.Res.Commun.206. 439-448 (1995)
[文献書誌] Araki,K.: "Purification and characterization of Rab GDIβ from rat brain." Biochem.Biophys.Res.Commun.211. 296-305 (1995)
[文献書誌] Takahashi,K.: "Heterotetramer formation of prenylated Rab3A with two Rabphilin-3A molecules." Biochem.Biophys.Res.Commun.217. 979-986 (1995)
[文献書誌] Sakaguchi,G.: "Molecular cloning of an isoform of Doc2 having two C_2-like domains." Biochem.Biophys.Res.Commun.217. 1053-1061 (1995)
[文献書誌] Miyazaki,M.: "Rabphilin-3A binds to a Mr 115,000 polypeptide in a phosphatidylserine-and Ca^<2+>-dependent manner." Mol.Brain.Res.28. 29-36 (1995)
[文献書誌] Regazzi,R.: "Prenylcysteine analogs mimicking the C-terminus of GTP-binding proteins stimulate exocytosis from permeabilized HIT-T15 cells-comparison with the effect of Rab3AL peptide." Biochim.Biophys.Acta. 1268. 269-278 (1995)
[文献書誌] Takai,Y.: "Rho as a regulator of the cytoskeleton." Trends in Biochemical Sciences. 20. 227-231 (1995)
[文献書誌] Ohtsuka,T.: "Activation of brain B-Raf protein kinase by RaplB small GTP-binding protein." J.Biol.Chem.271. 1258-1261 (1996)
[文献書誌] Orita,S.: "Doc2 enhances Ca^<2+>-dependent exocytosis from PC12 cells." J.Biol.Chem.271. 7257-7260 (1996)
[文献書誌] Fujita,Y.: "Phosphorylation of Munc-18/n-Secl/rbSecl by protein kinase C-Its implication in regulating the interaction of Munc-18/n-Secl/rbSecl with Syntaxin." J.Biol.Chem.271. 7265-7268 (1996)
[文献書誌] Kuroda,S.: "Different effects of various phospholipids on Ki-Ras-,Ha-Ras-,and Rap1B-induced B-Raf activation." J.Biol.Chem.271. 14680-14683 (1996)
[文献書誌] Shimizu,K.: "SMAP,an Smg GDS-associating protein having Arm repeats and phosphorylated by Src tyrosine kinese." J.Biol.Chem.271. 27013-27017 (1996)
[文献書誌] Kato,M.: "Physical and functional interaction of Rabphilin-3A with α-Actinin." J.Biol.Chem.271. 31775-31778 (1996)
[文献書誌] Hirano,H.: "ROM7/BEM4 encodes a novel protein that interacts with the Rholp small GTP-binding protein in Saccharomyces ceravisiae." Mol.Cell.Biol.16. 4396-4403 (1996)
[文献書誌] Hirao,M.: "Regulation mechanism of ERM(Ezrin/Radixin/Moesin)protein/plasma membrane association-Possible involvement of phosphatldyiinositol turnover and Rho-dependent signaling pathway." J.Cell.Biol.135. 37-51 (1996)
[文献書誌] Masumoto,N.: "Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs." J.Cell.Biol.135. 1741-1747 (1996)
[文献書誌] Drgonova,J.: "Rholp,a yeast protein at the interface between cell polarization and morphogenesis." Science. 272. 277-279 (1996)
[文献書誌] Ozaki,K.: "Rom1p and Rom2p are GDP/GTP exchange proteins(GEPs)for the Rholp small GTP binding protein in Saccharomyces cerevisiae." EMBO J.15. 2196-2207 (1996)
[文献書誌] Kohno,H.: "Bnilp implicated in cytoskeletal control is a putative target of Rholp small GTP binding protein in Saccharomyces cerevisiae." EMBO J.15. 6060-6068 (1996)
[文献書誌] Matsuda,S.: "A membrane-associated GDP/GTP exchange protein specific for Rho small GTP-binding protein-Partial purification and characterization from rat brain." Oncogene. 12. 915-920 (1996)
[文献書誌] Taniguchi,T.: "Cholecystokinin-B/gastrin receptors mediate repid formation of actin stress fibers." Oncogene. 12. 1357-1360 (1996)
[文献書誌] Komura,R.: "Involvement of rabphilin-3A in Ca^<2+>-dependent exocytosis from PC12 cells." Biochem.Biophys.Res.Commun.219. 435-440 (1996)
[文献書誌] Hotta,K.: "Interaction of the Rho family small G proteins with kinectin,an anchoring protein of kinesin motor." Biochem.Biophys.Res.Commun.225. 69-74 (1996)
[文献書誌] Senbonmatsu,T.: "Interaction of Rabphilin3 with aynaptic vesicles through multiple regions." Biochem.Biophys.Res.Commun.228. 567-572 (1996)
[文献書誌] Oishi,H.: "Interaction of both the C2A and C2B domains of rabphilin3 with Ca^<2+> and pospholipid." Biochem.Biophys.Res.Commun.229. 498-503 (1996)
[文献書誌] Hori,Y.: "Possible involvement of Rabll p24,a ras-like small GTP-binding protein,in intracellular vesicular transport of isolated pancreatic acini." Digestive Diseases and Sciences. 41. 133-138 (1996)
[文献書誌] Regazzi,R.: "Expression,localization and functional role of small GTPases of the Rab3 family in insulinsecreting cells." J.Cell.Sci.109. 2265-2273 (1996)
[文献書誌] Komuro,R.: "Involvement of Rho and Rac small G proetins and Rho GDI in Ca^<2+>-dependent exocytosis from PC12 cells." Genes to Cells. 1. 943-951 (1996)
[文献書誌] Takai,Y.: "Rab3A amall GTP-binding protein in Ca^<2+> -dependent exocytosis." Genes to Cells. 1. 615-632 (1996)
[文献書誌] Wada,M.: "Isolation and characterization of a GDP/GTP exchange protein specific for the Rab3 subfamily small G proteins." J.Biol.Chem.272. 3875-3878 (1997)
[文献書誌] Fukui,K.: "Isolation and characterization of a GDPase activating protein specific for the Rab3 subfamily of small G proteins." J.Biol.Chem.272. 4655-4658 (1997)
[文献書誌] Takeuchi,M.: "SAPAPs-A family of PSD-95/SAP90-associated proteins localized at postsynaptic density." J.Biol.Chem.(in press). (1997)
[文献書誌] Imamura,H.: "Bnilp and Bnrlp-Downstream targets of the Rho family small G proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae." EMBO J.(in press). (1997)
[文献書誌] Kotani,H.: "Rho regulates association of both the ERM family and vinculin with the plasma membrane in MDCK cells." Oncogene. (in press). (1997)
[文献書誌] Naito,A.: "Molecular cloning of mouse Doc2α and distribution of its mRNA in adult mouse brain." Mol.Brain.Res.(in press). (1997)
[文献書誌] Jin-no,Y.: "A novel function of the C-terminal liped moieties of Rab3A small G protein implicated in Ca^<2+>-dependent exocytosis-Inhibition of interaction with GTP and reduction of this inhibition by phospholipid." Genes to Cells. (in press). (1997)
[文献書誌] Koch,G.A.: "Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor(Rho GDI)in Saccharomyces cerevisiae." Oncogene. (in press). (1997)

1996 年度 実績報告書

低分子量G蛋白質による細胞機能の制御機構

研究代表者

高井 義美 大阪大学, 医学部, 教授 (60093514)

研究成果

[文献書誌] Miki,H.: "Association of Ash/Grb-2 with dynamin through the Src homology 3 domain." J.Biol.Chem.269. 5489-5492 (1994)

[文献書誌] Nishimura,N.: "Molecular cloning and characterization of two rab GDI species from rat brain-Brain-specific and ubiquitous types." J.Biol.Chem.269. 14191-14198 (1994)

[文献書誌] Nakanishi,H.: "Different functions of smg GDP dissociation stimulator and mammalian counterpart of yeast Cdc25." J.Biol.Chem.269. 15085-15091 (1994)

[文献書誌] Masuda,T.: "Molecular cloning and characterization of yeast rho GDP dissociation inhibitor." J.Biol.Chem.269. 19173-19718 (1994)

[文献書誌] Shimizu,K.: "Synergistic activation by Ras and 14-3-3 protein of a mitogen-activated protein kinase kinase kinase named Ras-dependent extracellular signal-regulated kinase kinase stimulator." J.Biol.Chem.269. 22917-22920 (1994)

[文献書誌] Shirataki,H.: "Rabphilin-3A is associated with synaptic vesicle through a vesicle protein in a manner independent of Rab3A." J.Biol.Chem.269. 32717-32720 (1994)

[文献書誌] Nishiyama,T.: "Rac p21 is involved in insulin-induced membrane ruffling and rho p21 is involved in hepatocyte growth factor and 12-O-tetradecanoylphorbol-13-acetate(TPA)-induced membrane ruffling in KB cells." Mol.Cell.Biol.14. 2447-2456 (1994)

[文献書誌] Yamochi,W.: "Growth site localization of Rhol small GTP-binding protein and its involvement in bud formation in Saccharomyces cerevisiae." J.Cell.Biol.125. 1077-1093 (1994)

[文献書誌] Huber,L.A.: "Mapping of ras-ralated GTP-binding proteins by GTP overlay following two-dimensional gel electrophoresis." Proc.Natl.Acad.Sci.USA. 91. 7874-7878 (1994)

[文献書誌] Takaishi,K.: "Involvement of Rho p21 small GTP-binding protein and its regulator in the HGF-induced cell motility." Oncogene. 9. 273-279 (1994)

[文献書誌] Wada,K.: "Localization of Rabphilin-3A,a putative target for Rab3A,at the aites of Ca^<2+>-dependent cxocytosis in Pc12 cells." Biochem.Biophys.Res.Commun.198. 158-165 (1994)

[文献書誌] Yaku,H.: "The Db1 oncogene product as a GDP/GTP exchange protein for the family-Its properties in comparison with those of Smg GDS." Biochem.Biophys.Res.Commun.198. 811-818 (1994)

[文献書誌] Nakanishi,H.: "Kinetic proerties of Ash/Grb2-interacting GDP/GTP exchange protein." Biochem.Biophys.Res.Commun.198. 1255-1261 (1994)

[文献書誌] Kim.Y.S.: "Phosphoprylation and activation of mitogen-activated protein kinase by kainic acid-induced seizure in rat hippocampus." Biochem.Biophys.Res.Commun.202. 1163-1168 (1994)

[文献書誌] Mizoguchi,A.: "Localization of Rabphilin-3A on the synaptic vesicle." Biochem.Biophys.Res.Commun.202. 1235-1243 (1994)

[文献書誌] Numata,S.: "Phosphorylation of Rabphilin-3A,a putative target protein for Rab3A,by cyclic AMP-dependent protein kinase." Biochem.Biophys.Res.Commun.203. 1927-1934 (1994)

[文献書誌] Miyazaki,M.: "Identification as β-adducin of a protein interacting with Rabphilin-3A in the presence of Ca^<2+> and phosphatidylserine." Biochem.Biophys.Res.Commun.205. 460-466 (1994)

[文献書誌] Imazumi,K.: "Identification of a Rabphilin-3A-interacting protein as GTP cyclohydorase I in PC12 cells." Biochem.Biophys.Res.Commun.205. 1409-1416 (1994)

[文献書誌] Kato.M.: "Phosphorylation of Rabphilin-3A by calmodulin-dependent protein kinese II." Biochem.Biophys.Res.Commun.205. 1776-1784 (1994)

[文献書誌] Miyazaki,A.: "Comparison of kinetic properties between MSS4 and Pab3A GRF GDP/GTP exchange proteins." FEBS Lett.350. 333-336 (1994)

[文献書誌] Fujita,Y.: "GDP/GTP exchange reaction-atimulating activity of Rabphilin-3A for Rab3A small GTP-binding protein." FEBS Lett.353. 67-70 (1994)

[文献書誌] Kuroda,S.: "Purification and characterization of REKS from Xenopus eggs-Identification of REKS as a Rasdependent mitogen-activated protein kinase kinese kinese" J.Biol.Chem.270. 2460-2465 (1995)

[文献書誌] Bourgoin,S.: "Low molecular weight GTP-binding proteins in HL-60 granulocytes." J.Biol.Chem.270. 3172-3178 (1995)

[文献書誌] Yamamori,B.: "Purification of a Ras-dependent mitogen-activated protein kinase kinase kinase from bovine brain cytosol and its identification as a complex of B-raf and 14-3-3 proteins." J.Biol.Chem.270. 11723-11726 (1995)

[文献書誌] Chung,S-H.: "Evidence that the Rab3A-binding protein,Rabphilin-3A,enhances regulated secretion-Studies in adrenal chromaffin cells." J.Biol.Chem.270. 16714-16718 (1995)

[文献書誌] Kuribara,H.: "Synergistic activation of rat brain phosholipase D by ADP-ribosylation factor and rhoA p21,and its inhibition by clostridium botulinum C3 exoenzyme." J.Biol.Chem.270. 25667-25671 (1995)

[文献書誌] McGeady,P.: "The farnesyl group of H-ras facilitates the activation of a soluble upstream activator of mitogen-activated protein kinase." J.Biol.Chem.270. 26347-26351 (1995)

[文献書誌] Gotoh,T.: "Identification of Rapl as a target for the Crk SH3 Domain-binding guanine nucleotide-releasing factor C3G." Mol.Cell.Biol.15. 6746-6753 (1995)

[文献書誌] Nonaka,H.: "A downstream target of RHO1 small GTP-binding protein is PKC1,a homolog of protein kinase C,which leads to activation of the MAP kinase cascade in Saccharomyces cerevisiae." EMBO.J.14. 5931-5938 (1995)

[文献書誌] Takaishi,K.: "Translocation of activated Rho from the cytoplasm to membrane ruffling area,cell-cell adhesion sites and cleavage furrows." Oncogene. 11. 39-48 (1995)

[文献書誌] Nishimura,N.: "Cloning of a brain-type isoform of human rab GDI and its expression in human neuroblastoma cell lines and tumor specimens." Cancer Res.55. 5445-5450 (1995)

[文献書誌] Orita,S.: "Doc2: a novel brain protein having two repeated C_2-like domains." Biochem.Biophys.Res.Commun.206. 439-448 (1995)

[文献書誌] Araki,K.: "Purification and characterization of Rab GDIβ from rat brain." Biochem.Biophys.Res.Commun.211. 296-305 (1995)

[文献書誌] Takahashi,K.: "Heterotetramer formation of prenylated Rab3A with two Rabphilin-3A molecules." Biochem.Biophys.Res.Commun.217. 979-986 (1995)

[文献書誌] Sakaguchi,G.: "Molecular cloning of an isoform of Doc2 having two C_2-like domains." Biochem.Biophys.Res.Commun.217. 1053-1061 (1995)

[文献書誌] Miyazaki,M.: "Rabphilin-3A binds to a Mr 115,000 polypeptide in a phosphatidylserine-and Ca^<2+>-dependent manner." Mol.Brain.Res.28. 29-36 (1995)

[文献書誌] Regazzi,R.: "Prenylcysteine analogs mimicking the C-terminus of GTP-binding proteins stimulate exocytosis from permeabilized HIT-T15 cells-comparison with the effect of Rab3AL peptide." Biochim.Biophys.Acta. 1268. 269-278 (1995)

[文献書誌] Takai,Y.: "Rho as a regulator of the cytoskeleton." Trends in Biochemical Sciences. 20. 227-231 (1995)

[文献書誌] Ohtsuka,T.: "Activation of brain B-Raf protein kinase by RaplB small GTP-binding protein." J.Biol.Chem.271. 1258-1261 (1996)

[文献書誌] Orita,S.: "Doc2 enhances Ca^<2+>-dependent exocytosis from PC12 cells." J.Biol.Chem.271. 7257-7260 (1996)

[文献書誌] Fujita,Y.: "Phosphorylation of Munc-18/n-Secl/rbSecl by protein kinase C-Its implication in regulating the interaction of Munc-18/n-Secl/rbSecl with Syntaxin." J.Biol.Chem.271. 7265-7268 (1996)

[文献書誌] Kuroda,S.: "Different effects of various phospholipids on Ki-Ras-,Ha-Ras-,and Rap1B-induced B-Raf activation." J.Biol.Chem.271. 14680-14683 (1996)

[文献書誌] Shimizu,K.: "SMAP,an Smg GDS-associating protein having Arm repeats and phosphorylated by Src tyrosine kinese." J.Biol.Chem.271. 27013-27017 (1996)

[文献書誌] Kato,M.: "Physical and functional interaction of Rabphilin-3A with α-Actinin." J.Biol.Chem.271. 31775-31778 (1996)

[文献書誌] Hirano,H.: "ROM7/BEM4 encodes a novel protein that interacts with the Rholp small GTP-binding protein in Saccharomyces ceravisiae." Mol.Cell.Biol.16. 4396-4403 (1996)

[文献書誌] Hirao,M.: "Regulation mechanism of ERM(Ezrin/Radixin/Moesin)protein/plasma membrane association-Possible involvement of phosphatldyiinositol turnover and Rho-dependent signaling pathway." J.Cell.Biol.135. 37-51 (1996)

[文献書誌] Masumoto,N.: "Involvement of Rabphilin-3A in cortical granule exocytosis in mouse eggs." J.Cell.Biol.135. 1741-1747 (1996)

[文献書誌] Drgonova,J.: "Rholp,a yeast protein at the interface between cell polarization and morphogenesis." Science. 272. 277-279 (1996)

[文献書誌] Ozaki,K.: "Rom1p and Rom2p are GDP/GTP exchange proteins(GEPs)for the Rholp small GTP binding protein in Saccharomyces cerevisiae." EMBO J.15. 2196-2207 (1996)

[文献書誌] Kohno,H.: "Bnilp implicated in cytoskeletal control is a putative target of Rholp small GTP binding protein in Saccharomyces cerevisiae." EMBO J.15. 6060-6068 (1996)

[文献書誌] Matsuda,S.: "A membrane-associated GDP/GTP exchange protein specific for Rho small GTP-binding protein-Partial purification and characterization from rat brain." Oncogene. 12. 915-920 (1996)

[文献書誌] Taniguchi,T.: "Cholecystokinin-B/gastrin receptors mediate repid formation of actin stress fibers." Oncogene. 12. 1357-1360 (1996)

[文献書誌] Komura,R.: "Involvement of rabphilin-3A in Ca^<2+>-dependent exocytosis from PC12 cells." Biochem.Biophys.Res.Commun.219. 435-440 (1996)

[文献書誌] Hotta,K.: "Interaction of the Rho family small G proteins with kinectin,an anchoring protein of kinesin motor." Biochem.Biophys.Res.Commun.225. 69-74 (1996)

[文献書誌] Senbonmatsu,T.: "Interaction of Rabphilin3 with aynaptic vesicles through multiple regions." Biochem.Biophys.Res.Commun.228. 567-572 (1996)

[文献書誌] Oishi,H.: "Interaction of both the C2A and C2B domains of rabphilin3 with Ca^<2+> and pospholipid." Biochem.Biophys.Res.Commun.229. 498-503 (1996)

[文献書誌] Hori,Y.: "Possible involvement of Rabll p24,a ras-like small GTP-binding protein,in intracellular vesicular transport of isolated pancreatic acini." Digestive Diseases and Sciences. 41. 133-138 (1996)

[文献書誌] Regazzi,R.: "Expression,localization and functional role of small GTPases of the Rab3 family in insulinsecreting cells." J.Cell.Sci.109. 2265-2273 (1996)

[文献書誌] Komuro,R.: "Involvement of Rho and Rac small G proetins and Rho GDI in Ca^<2+>-dependent exocytosis from PC12 cells." Genes to Cells. 1. 943-951 (1996)

[文献書誌] Takai,Y.: "Rab3A amall GTP-binding protein in Ca^<2+> -dependent exocytosis." Genes to Cells. 1. 615-632 (1996)

[文献書誌] Wada,M.: "Isolation and characterization of a GDP/GTP exchange protein specific for the Rab3 subfamily small G proteins." J.Biol.Chem.272. 3875-3878 (1997)

[文献書誌] Fukui,K.: "Isolation and characterization of a GDPase activating protein specific for the Rab3 subfamily of small G proteins." J.Biol.Chem.272. 4655-4658 (1997)

[文献書誌] Takeuchi,M.: "SAPAPs-A family of PSD-95/SAP90-associated proteins localized at postsynaptic density." J.Biol.Chem.(in press). (1997)

[文献書誌] Imamura,H.: "Bnilp and Bnrlp-Downstream targets of the Rho family small G proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae." EMBO J.(in press). (1997)

[文献書誌] Kotani,H.: "Rho regulates association of both the ERM family and vinculin with the plasma membrane in MDCK cells." Oncogene. (in press). (1997)

[文献書誌] Naito,A.: "Molecular cloning of mouse Doc2α and distribution of its mRNA in adult mouse brain." Mol.Brain.Res.(in press). (1997)

[文献書誌] Jin-no,Y.: "A novel function of the C-terminal liped moieties of Rab3A small G protein implicated in Ca^<2+>-dependent exocytosis-Inhibition of interaction with GTP and reduction of this inhibition by phospholipid." Genes to Cells. (in press). (1997)

[文献書誌] Koch,G.A.: "Association of the Rho family small GTP-binding proteins with Rho GDP dissociation inhibitor(Rho GDI)in Saccharomyces cerevisiae." Oncogene. (in press). (1997)

1996 年度実績報告書

高井義美大阪大学, 医学部, 教授 (60093514)