1994 年度研究成果報告書概要

発生・形態形成の個体レベルにおける分子生物学的研究

研究課題

研究課題/領域番号	06NP0901
研究種目	創成的基礎研究費
配分区分	補助金
研究機関	京都大学
研究代表者	竹市雅俊京都大学, 理学部, 教授 (00025454)
研究分担者	松居靖久東北大学, 加齢医学研究所, 助教授 (40241575) 山村研一熊本大学, 医学部, 教授 (90115197) 相沢慎一熊本大学, 医学部, 教授 (60073011) 黒岩厚名古屋大学, 理学部, 教授 (20134611) 安田國雄国立奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (30025473)
研究期間 (年度)	1993 – 1997
キーワード	発生 / 遺伝子タ-デッティング / 形態形成 / 細胞分化 / 細胞認識 / 神経発生 / 遺伝子トラッピング / ES細胞
研究概要	1.脳におけるカドヘリンの発現パターンの詳細なマッピングを継続し、次の結果を得た。まず、初期胚の脳において複数のカドヘリンがニューロメアという構造単位を規定するように発現し、脳の分節化に重要な役割を果たしていることの証拠を得た。次に、カドヘリンがシナプスに存在する間接証拠を得た。これにより、カドヘリンが神経ネットワーク形成の鍵となる可能性が一層高まった。一方、ショウジョウバエの神経系において発現するカドヘリンを新たに同定し、その機能を分子遺伝学的に解明する手掛りを得た。 2.ニワトリαAクリスタリン遺伝子のエンハンサーに結合するL-mafの発現分布を調べた結果、将来水晶体に分化する外胚葉領域で発現が始まり、しかも体全体の中でこの領域だけが陽性であった。この発見は、L-mafが水晶体分化の中心的制御因子である可能性を示唆している。 3.始原生殖細胞由来EG細胞を生殖細胞欠失マウスに移植すると、生殖細胞特異的なマーカーにより認識される細胞が確認され、EG細胞から生殖細胞が誘導される可能性が示唆された。このシステムが確立すれば、生殖系の遺伝子操作が容易となりその意義は大きい。 4.RNA結合蛋白質musashi遺伝子がマウス中枢神経系の幹細胞特異的に発現していることを明らかにした。 5.ショウジョウバエの筋肉表面因子コネクチンをすべての筋肉で異所的に発現させることにより、神経-筋肉間の誘因的な認識分子として機能し得ることを明らかにした。また、F-スポンジンと相同性がある分子が一部の筋肉だけで発現されることを明らかにした。 6.消化管におけるHoxBクラスターの発現を調べ、特に肺において構造的区画及びBMP4,Wnt5aなどのシグナル因子の発現と相関する興味深い発現パターンを発見した。 7.Fak欠損マウスから細胞株を樹立し、細胞の移動能の低下、focal contactの欠失、microspikeの形成異常などを見い出した。また、Otx2欠損マウスを解析し、頭部の吻側、尾側の構造異常を認め、その頭部形成上の役割を証明した。 8.神経前駆細胞で発現するHES-1のノックアウトマウスを作製し解析したところ、無脳症になること、また、神経分化が早熟的に起きることがわかった。 9.初期発生の異常を引き起こすjmj遺伝子の構造を明らかにすると共に、新たに、胸腺、背部結合組織の異常を発見した。 10.マウス原腸形成に関与する遺伝子のクローニングのためにPCRディファレンシャルディスプレイ法を適用し、部域特異的に発現される遺伝子がクローニングできることが確認された。

研究成果

(74件)

すべてその他

すべて文献書誌 (74件)

[文献書誌] Oda,H.: "A Drosophila homolog of cadherin associated with Armadillo and essential for embryonic cell-cell adhesion." Develop.Biol.165. 716-726 (1994)
[文献書誌] Hatta,M.: "Complex cell type-specific transcriptional regulation by the promoter and an intron of the mouse P-cadherin gene." Develop.Growth & Differ.36. 509-519 (1994)
[文献書誌] Oyama,T.: "A truncated B-catenin disrupts the interaction between E-cadherin and α-catenin:A cause of loss of intercellular adhesiveness in human cancer cell lines." Cancer Res.54. 6282-6287 (1995)
[文献書誌] Shibamoto,S.: "Association of p120,a tyrosine kinase substrate,with E-cadherin/catenin complexes." J.Cell Biol.(in press). (1995)
[文献書誌] Overduin,M.: "Three-dimemsional solution structure and calcium interaction of the epithelial cadherin domain responsible for selective adhesion." Science. 267. 386-389 (1995)
[文献書誌] Nakagawa,S.: "Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific exoression of novel cadherins." Development. (in press). (1995)
[文献書誌] Shibamoto,S.: "Tyrosine phosphorylation of β-catenin and plakoglobin enhanced by hepatocyte growth factor and epidermal growth factor in human carcinoma cells." Cell Adhesion & Commun.1. 295-305 (1994)
[文献書誌] Shiomi,K.: "Alternative cell fate choice induced by low-level expression of a regulator of protein phosphatase 2A in the Drosophila peripheral nervous system." Development. 120. 1591-1599 (1994)
[文献書誌] Uchiyama,N.: "Immunoelectron microscopic localization of E-cadherin in dorsal root ganglia,dorsal root and dorsal horn of postnatal mice." J.Neurocytol.23. 460-468 (1994)
[文献書誌] Shimamura,K.: "Wnt-1-dependent regulation of local E-cadherin and αN-catenin expression in the embryonic mouse brain." Development. 120. 2225-2234 (1994)
[文献書誌] Watabe,M.: "Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersedcarcinoma line." J.Cell Biol.127. 247-256 (1994)
[文献書誌] Nose,A.: "Ectopic expression of connection reveals a repulsive function during growth cone guidance and synapticformation." Neuron. 13. 525-539 (1994)
[文献書誌] Ishizaki,K.,: "IncreasedUV-induced SCEs in p53-.deficient mouse cells." Int.J.Cancer.58. 254-257 (1994)
[文献書誌] Tamemoto,H.,: "Targeted disruption of the insulin receptor substrate-1 gene results in insulin resistance and growth retardation." Nature. 372. 182-186 (1994)
[文献書誌] Nada,S.,: "Identification of majortyrosine-phosphorylatedproteins in Csk-deficient cells." Oncogene. 9. 3571-3578 (1994)
[文献書誌] Tojo,H.,: "Targeted Disruption of the neurotrophin-3 gene with lac Z induces loss of trk C-positive neurons in sensory ganglia but not in spinal cords" Brain Research.669. 163-175 (1994)
[文献書誌] Sakimura,K.,: "Reduced hippocampalLTP and spatiallearning inmice lacking NMDA receptor el subunit." Nature. (in press). (1994)
[文献書誌] Yoshida,M.,: "A new strategy of gene-trapping in ES cells using 3'RACE." Transgenic Research. (in press). (1994)
[文献書誌] Umemori,H.,: "Initial events of myelination involve Fyn tyrosine kinase signalling." Nature. 367. 572-576 (1994)
[文献書誌] Kubo,S.,: "A novel form of self tolerance dictated in the thymus of transgenic mice with autoreactive TCR a and b chain genes." Internatl.Immunol.6. 593-602 (1994)
[文献書誌] Hashimoto,N.,: "Parthenogenetic activation of oocytes,but normal spermatogenesis and somatic cell growth,by c-mos-deficiency in mice." Nature. 370. 68-71 (1994)
[文献書誌] Yagi,T.,: "Fyn expression during neurogenesis in mouse embryos." Oncogene. 9. 2433-2440 (1994)
[文献書誌] Tanaka,N.,: "Cellular commitment to oncogene-induced transformation or apoptosisis dependent on the transcription factor IRF-1." Cell. 77. 829-839 (1994)
[文献書誌] Kohmura,N.,: "A novel non-receptor tyrosine kinase,srm;cloning and targeted disruption." Mol.Cell.Biol.14. 6915-6925 (1994)
[文献書誌] Ilic,D.,: "Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells." Biochem.Biophys.Res.Commun. (in press). (1994)
[文献書誌] Hu,L.,: "P53 controls proliferation of early B lineage cells by a p21(WAF1/CIP1)-independent pathway." Biochem.Biophys.Res.Commun. 372. 182-186 (1994)
[文献書誌] Aizawa,S.,et al.: "Non-receptor tyrosine kinases in mammalian neurogenesis" Proceedings of the 24th International Symposium of the Princess Takamatsu Cancer Research Fund. 323-337 (1994)
[文献書誌] R.Sekido,: "δ-Crystallin enhancer binding protein δEF1 is a repressor of E2-box-mediated gene activation." Molecular and Cellular Biology. 14. 5692-5700 (1994)
[文献書誌] Y.Takahashi,: "Lens-specific activity of the chicken δ1-crystallin enhancer in the mouse." International Journal of Developmental Biology. 38. 365-368 (1994)
[文献書誌] S.Kamada,: "bcl-2 deficiency in mice leads to pleiotropic abnormalities:polycystic kidney etc." Cancer Research. 55. 354-359 (1995)
[文献書誌] Marshall,H.: "A conserved retinoic acid response element required for early expression of the homeobox gene Hoxb-1." Nature.370. 567-571 (1994)
[文献書誌] Studer,M.: "Role of a conserved retinoic acid response element in rhombomere-restriction of Hoxb-1." Science. 265. 1728-1731 (1994)
[文献書誌] Yokouchi,Y.: "Coordinated expression of Abd-B subfamily genes of the HoxA cluster in the developing digestive tract of chick embryo." Developmental Biology. 168(in press). (1995)
[文献書誌] Coffey,R.J.,: "Acceleration of mammary neoplasia in transforming growth factor α transgenic mice by 7,1-dimethylbenzanthracene." Cancer Research. 54. 1678-1683 (1994)
[文献書誌] Terajima,M.,: "Structural organization of the mouse glycophorin A gene." J.Biochem.116. 1105-1110 (1994)
[文献書誌] Kato,S.,: "Molecular cloning and expression of mouse Mg^<2+>・dependent protein phosphatase β-4(type2Cβ-4)." Arch.Biochem.Biophys.(in press). (1995)
[文献書誌] Matsubara,N.,: "Murine polo like kinase 1 gene is expressed in mciotic testicular germ cells and oocytes." Mol.Reprod.Dev.(in press). (1995)
[文献書誌] Nishiyori,A.,: "Determination of tissue specificity of the enhancer by combinatorial operation of tissue-enriched transcription factors." J.Biol.Chem.269. 1323-1331 (1994)
[文献書誌] Toyonaga,T.,: "Chronic active hepatltis in transgenic mice expressing interferon-γ in the liver." Proc.Natl.Acad.Sci.USA.91. 614-618 (1994)
[文献書誌] Kaname,T.,: "The upstream sequence of a new growth/differentiation factor,midkine(MK),mediates developmentally regulated lacZ gene expression in transgenic mice." Devlop.Growth Diifer.36. 231-238 (1994)
[文献書誌] Yamashita,S.,: "Group II phospholipase A2 in invasive gastric cancer cell line is induced by interleukin 6." Biochem.Biophys.Res.Com.198. 878-884 (1994)
[文献書誌] Nishiyama,Y.,: "Expression of the gene encoding the tyrosine kinase-deflcient human insulin receptor in transgenic mcie." 141. 187-192 (1994)
[文献書誌] Imamura,F.,: "The murine interleukin-5 receptor a-subunit gene:characterization of the gene Structure and chrimosome mapping." DNA Cell Biol.13. 283-292 (1994)
[文献書誌] Araki,S.,Yi: "Systemic amyloidosis in transgenic mice carrying the human mutant transthyretin(Me130)gene." Mol.Neurobiol.8. 15-23 (1994)
[文献書誌] Tashiro,F.,: "Resistance to cyclophosphamide-induced diabetes in transgenic NOD mice expressing l-A^k." Autolmmunity. 17. 181-188 (1994)
[文献書誌] Mantani,A.,: "A novel isoform of the neurofibromatosis type-1 mRNA and a switch of isoforms during murine cell differentiation and proliferation." Gene. 148. 245-251 (1994)
[文献書誌] Kimura,S.,: "Improvement of germ line transmission by targeting β-galactosidase to nuclei in transgenic mice." Devel.Growh Different.36. 521-527 (1994)
[文献書誌] 能瀬聡直: "Ectopic expression of Connectin reveals a repulsive function during growth cone guidance and synapse formation." Neuron. 13. 535-539 (1994)
[文献書誌] Nakamura,M.,: "MUSASHI,a neuronal protein required for Drosophlla adult external sensory organ development." Neuron. 13. 67-81 (1994)
[文献書誌] Kagawa,T.,: "Glial cell degeneration and hypomyelination caused by overexpression of the myelin proteollpid protein gene:implication for roles of the gene in differentiation and survival." Neuron. 13. 427-442 (1994)
[文献書誌] Xiong.W.C.,: "repo encodes a glial-specific homeodomain protein required in the Drosophila nervous system." Genes and Development. 8. 981-994 (1994)
[文献書誌] Okano,H.: "Cloning of a Drosophila melanogaster homologue of the mouse type-1BMP-2/-4 receptor:a potentlal decapentaplegic receptor." Gene. 148. 203-209 (1994)
[文献書誌] Sawamoto,K.,: "The function of argos in regulating cell fate decisions during the Drosophila eye and wing vein development." Developmental Blology. 164. 267-276 (1994)
[文献書誌] 安田國雄: "Functional conservation of the βB1-crystallin promoter between mouse and chicken." Develop.Biol.(in press). (1995)
[文献書誌] Makoto Ishihashi: "Persistent expression of helix-loop-helix factor HES-1 prevents mammalian neural differentiation in the central nervous system." EMBO Journal. 13. 1799-1805 (1994)
[文献書誌] Koichi Takebayashi,: "Structure,chromosomal locus,and promoter analysis of the gene encoding the mouse helix-loop-helix factorHES-1:negative autoregulation through the multiple N box elements." The Journal of Biological Chemistry. 269. 5150-5156 (1994)
[文献書誌] Teiji Sakagami,: "Structure and chromosomal locus of the mouse gene encoding a cerebellar Purkinje cell-specific helix-loop-helix factor HES-3." Biochemical and Biophysical Research Communications. 203. 594-601 (1994)
[文献書誌] Koichi Takebayashi,: "Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5:identification of the neural precursor cell-specific promoter element." The Journal of Biological Chemistry. 270. 1342-1349 (1995)
[文献書誌] Chihiro Akazawa,: "A mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal is a positive transcriptional regulator expressed in the developing nervous system." The Journal of Biological Chemistry. (in press). (1995)
[文献書誌] Chikara Shimizu,: "MATH-2,a mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal,is specifically expressed in the nervous system." European Journal of Biochemistry. (in press). (1995)
[文献書誌] Takahashi,M.,: "Molecular heterogeneity among primary motoneurons and within myotomes revealed by the differential mRNA expression of novel Islet-1 homologs in embryonic zebrafish." (submitted to Dev.Biol.).
[文献書誌] Inoue,A.,: "Developmental regulation of Isl-1 mRNA expression during neuronal differentation in embryonic zebrafish." Devel.Dynamics,. 197. 1-11 (1994)
[文献書誌] 岡本仁: "脳の形成はいかに遺伝子によって決定され、制御されているか(特集神経科学の謎)" 生体の科学. 46. 2-7 (1995)
[文献書誌] 岡本仁: "ゼブラフィッシュ神経細胞の運命決定," プレインメヂカル,. 6. 135-142 (1994)
[文献書誌] 岡本仁: "ゼブラフィッシュの脊髄と運動神経細胞の分化〜転写因子と組織間相互作用の役割〜" 生体の科学. 45. 182-188 (1994)
[文献書誌] 竹内隆: "IRESを用いたマウス遺伝子トラップ法による神経管形成機構の解析" 組織培養. 21. 55-59 (1995)
[文献書誌] J.Cooke: "Experimental control of axial pattern in the chick blastoderm by localexpression of Wnt and activin:The role of HNK-1 positive cells." Developmental Biology. 164. 513-527 (1994)
[文献書誌] Shinji Takada: "Wnt-3a regulates somite andtailbud formation in the mouse embryo." Genes & Development. 8. 174-189 (1994)
[文献書誌] 相沢慎一: "バイオマニュアルシリーズ8 羊土社" ジーンターゲテイング:ES細胞を用いた変異マウスの作製, 174 (1994)
[文献書誌] 松居靖久: "最新・細胞内シグナル伝達ライブラリー(山本雅編)" 羊土社(印刷中), (1995)
[文献書誌] 松居靖久: "生殖細胞-基礎から最前線まで(岡田益吉・長濱嘉孝編)" 共立出版(印刷中), (1995)
[文献書誌] Okano,H.,: "Drosophila visual system as a model to study neurobiology in gencral:identification of genes regulating cell fate decision in ncural development.Basic Neuroscience in Invertebrate." Business Center for Academic Societies Japan,Ed II.Koike.(印刷中), (1995)
[文献書誌] Mikoshiba,K.,: "Molecular genetic analyses of the myelin deficiency and cerebellar ataxia in“Progress in Brain Research"" Elsevier(印刷中), (1995)
[文献書誌] 岡野栄之: "“モデル生物系を用いた中枢神経系の発生と分化の解析"脳機能の解明に向けて" 赤池紀扶ら編、創風社(印刷中), (1995)

1994 年度 研究成果報告書概要

発生・形態形成の個体レベルにおける分子生物学的研究

研究代表者

竹市 雅俊 京都大学, 理学部, 教授 (00025454)

研究成果

[文献書誌] Oda,H.: "A Drosophila homolog of cadherin associated with Armadillo and essential for embryonic cell-cell adhesion." Develop.Biol.165. 716-726 (1994)

[文献書誌] Hatta,M.: "Complex cell type-specific transcriptional regulation by the promoter and an intron of the mouse P-cadherin gene." Develop.Growth & Differ.36. 509-519 (1994)

[文献書誌] Oyama,T.: "A truncated B-catenin disrupts the interaction between E-cadherin and α-catenin:A cause of loss of intercellular adhesiveness in human cancer cell lines." Cancer Res.54. 6282-6287 (1995)

[文献書誌] Shibamoto,S.: "Association of p120,a tyrosine kinase substrate,with E-cadherin/catenin complexes." J.Cell Biol.(in press). (1995)

[文献書誌] Overduin,M.: "Three-dimemsional solution structure and calcium interaction of the epithelial cadherin domain responsible for selective adhesion." Science. 267. 386-389 (1995)

[文献書誌] Nakagawa,S.: "Neural crest cell-cell adhesion controlled by sequential and subpopulation-specific exoression of novel cadherins." Development. (in press). (1995)

[文献書誌] Shibamoto,S.: "Tyrosine phosphorylation of β-catenin and plakoglobin enhanced by hepatocyte growth factor and epidermal growth factor in human carcinoma cells." Cell Adhesion & Commun.1. 295-305 (1994)

[文献書誌] Shiomi,K.: "Alternative cell fate choice induced by low-level expression of a regulator of protein phosphatase 2A in the Drosophila peripheral nervous system." Development. 120. 1591-1599 (1994)

[文献書誌] Uchiyama,N.: "Immunoelectron microscopic localization of E-cadherin in dorsal root ganglia,dorsal root and dorsal horn of postnatal mice." J.Neurocytol.23. 460-468 (1994)

[文献書誌] Shimamura,K.: "Wnt-1-dependent regulation of local E-cadherin and αN-catenin expression in the embryonic mouse brain." Development. 120. 2225-2234 (1994)

[文献書誌] Watabe,M.: "Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersedcarcinoma line." J.Cell Biol.127. 247-256 (1994)

[文献書誌] Nose,A.: "Ectopic expression of connection reveals a repulsive function during growth cone guidance and synapticformation." Neuron. 13. 525-539 (1994)

[文献書誌] Ishizaki,K.,: "IncreasedUV-induced SCEs in p53-.deficient mouse cells." Int.J.Cancer.58. 254-257 (1994)

[文献書誌] Tamemoto,H.,: "Targeted disruption of the insulin receptor substrate-1 gene results in insulin resistance and growth retardation." Nature. 372. 182-186 (1994)

[文献書誌] Nada,S.,: "Identification of majortyrosine-phosphorylatedproteins in Csk-deficient cells." Oncogene. 9. 3571-3578 (1994)

[文献書誌] Tojo,H.,: "Targeted Disruption of the neurotrophin-3 gene with lac Z induces loss of trk C-positive neurons in sensory ganglia but not in spinal cords" Brain Research.669. 163-175 (1994)

[文献書誌] Sakimura,K.,: "Reduced hippocampalLTP and spatiallearning inmice lacking NMDA receptor el subunit." Nature. (in press). (1994)

[文献書誌] Yoshida,M.,: "A new strategy of gene-trapping in ES cells using 3'RACE." Transgenic Research. (in press). (1994)

[文献書誌] Umemori,H.,: "Initial events of myelination involve Fyn tyrosine kinase signalling." Nature. 367. 572-576 (1994)

[文献書誌] Kubo,S.,: "A novel form of self tolerance dictated in the thymus of transgenic mice with autoreactive TCR a and b chain genes." Internatl.Immunol.6. 593-602 (1994)

[文献書誌] Hashimoto,N.,: "Parthenogenetic activation of oocytes,but normal spermatogenesis and somatic cell growth,by c-mos-deficiency in mice." Nature. 370. 68-71 (1994)

[文献書誌] Yagi,T.,: "Fyn expression during neurogenesis in mouse embryos." Oncogene. 9. 2433-2440 (1994)

[文献書誌] Tanaka,N.,: "Cellular commitment to oncogene-induced transformation or apoptosisis dependent on the transcription factor IRF-1." Cell. 77. 829-839 (1994)

[文献書誌] Kohmura,N.,: "A novel non-receptor tyrosine kinase,srm;cloning and targeted disruption." Mol.Cell.Biol.14. 6915-6925 (1994)

[文献書誌] Ilic,D.,: "Focal adhesion kinase is not essential for in vitro and in vivo differentiation of ES cells." Biochem.Biophys.Res.Commun. (in press). (1994)

[文献書誌] Hu,L.,: "P53 controls proliferation of early B lineage cells by a p21(WAF1/CIP1)-independent pathway." Biochem.Biophys.Res.Commun. 372. 182-186 (1994)

[文献書誌] Aizawa,S.,et al.: "Non-receptor tyrosine kinases in mammalian neurogenesis" Proceedings of the 24th International Symposium of the Princess Takamatsu Cancer Research Fund. 323-337 (1994)

[文献書誌] R.Sekido,: "δ-Crystallin enhancer binding protein δEF1 is a repressor of E2-box-mediated gene activation." Molecular and Cellular Biology. 14. 5692-5700 (1994)

[文献書誌] Y.Takahashi,: "Lens-specific activity of the chicken δ1-crystallin enhancer in the mouse." International Journal of Developmental Biology. 38. 365-368 (1994)

[文献書誌] S.Kamada,: "bcl-2 deficiency in mice leads to pleiotropic abnormalities:polycystic kidney etc." Cancer Research. 55. 354-359 (1995)

[文献書誌] Marshall,H.: "A conserved retinoic acid response element required for early expression of the homeobox gene Hoxb-1." Nature.370. 567-571 (1994)

[文献書誌] Studer,M.: "Role of a conserved retinoic acid response element in rhombomere-restriction of Hoxb-1." Science. 265. 1728-1731 (1994)

[文献書誌] Yokouchi,Y.: "Coordinated expression of Abd-B subfamily genes of the HoxA cluster in the developing digestive tract of chick embryo." Developmental Biology. 168(in press). (1995)

[文献書誌] Coffey,R.J.,: "Acceleration of mammary neoplasia in transforming growth factor α transgenic mice by 7,1-dimethylbenzanthracene." Cancer Research. 54. 1678-1683 (1994)

[文献書誌] Terajima,M.,: "Structural organization of the mouse glycophorin A gene." J.Biochem.116. 1105-1110 (1994)

[文献書誌] Kato,S.,: "Molecular cloning and expression of mouse Mg^<2+>・dependent protein phosphatase β-4(type2Cβ-4)." Arch.Biochem.Biophys.(in press). (1995)

[文献書誌] Matsubara,N.,: "Murine polo like kinase 1 gene is expressed in mciotic testicular germ cells and oocytes." Mol.Reprod.Dev.(in press). (1995)

[文献書誌] Nishiyori,A.,: "Determination of tissue specificity of the enhancer by combinatorial operation of tissue-enriched transcription factors." J.Biol.Chem.269. 1323-1331 (1994)

[文献書誌] Toyonaga,T.,: "Chronic active hepatltis in transgenic mice expressing interferon-γ in the liver." Proc.Natl.Acad.Sci.USA.91. 614-618 (1994)

[文献書誌] Kaname,T.,: "The upstream sequence of a new growth/differentiation factor,midkine(MK),mediates developmentally regulated lacZ gene expression in transgenic mice." Devlop.Growth Diifer.36. 231-238 (1994)

[文献書誌] Yamashita,S.,: "Group II phospholipase A2 in invasive gastric cancer cell line is induced by interleukin 6." Biochem.Biophys.Res.Com.198. 878-884 (1994)

[文献書誌] Nishiyama,Y.,: "Expression of the gene encoding the tyrosine kinase-deflcient human insulin receptor in transgenic mcie." 141. 187-192 (1994)

[文献書誌] Imamura,F.,: "The murine interleukin-5 receptor a-subunit gene:characterization of the gene Structure and chrimosome mapping." DNA Cell Biol.13. 283-292 (1994)

[文献書誌] Araki,S.,Yi: "Systemic amyloidosis in transgenic mice carrying the human mutant transthyretin(Me130)gene." Mol.Neurobiol.8. 15-23 (1994)

[文献書誌] Tashiro,F.,: "Resistance to cyclophosphamide-induced diabetes in transgenic NOD mice expressing l-A^k." Autolmmunity. 17. 181-188 (1994)

[文献書誌] Mantani,A.,: "A novel isoform of the neurofibromatosis type-1 mRNA and a switch of isoforms during murine cell differentiation and proliferation." Gene. 148. 245-251 (1994)

[文献書誌] Kimura,S.,: "Improvement of germ line transmission by targeting β-galactosidase to nuclei in transgenic mice." Devel.Growh Different.36. 521-527 (1994)

[文献書誌] 能瀬聡直: "Ectopic expression of Connectin reveals a repulsive function during growth cone guidance and synapse formation." Neuron. 13. 535-539 (1994)

[文献書誌] Nakamura,M.,: "MUSASHI,a neuronal protein required for Drosophlla adult external sensory organ development." Neuron. 13. 67-81 (1994)

[文献書誌] Kagawa,T.,: "Glial cell degeneration and hypomyelination caused by overexpression of the myelin proteollpid protein gene:implication for roles of the gene in differentiation and survival." Neuron. 13. 427-442 (1994)

[文献書誌] Xiong.W.C.,: "repo encodes a glial-specific homeodomain protein required in the Drosophila nervous system." Genes and Development. 8. 981-994 (1994)

[文献書誌] Okano,H.: "Cloning of a Drosophila melanogaster homologue of the mouse type-1BMP-2/-4 receptor:a potentlal decapentaplegic receptor." Gene. 148. 203-209 (1994)

[文献書誌] Sawamoto,K.,: "The function of argos in regulating cell fate decisions during the Drosophila eye and wing vein development." Developmental Blology. 164. 267-276 (1994)

[文献書誌] 安田國雄: "Functional conservation of the βB1-crystallin promoter between mouse and chicken." Develop.Biol.(in press). (1995)

[文献書誌] Makoto Ishihashi: "Persistent expression of helix-loop-helix factor HES-1 prevents mammalian neural differentiation in the central nervous system." EMBO Journal. 13. 1799-1805 (1994)

[文献書誌] Koichi Takebayashi,: "Structure,chromosomal locus,and promoter analysis of the gene encoding the mouse helix-loop-helix factorHES-1:negative autoregulation through the multiple N box elements." The Journal of Biological Chemistry. 269. 5150-5156 (1994)

[文献書誌] Teiji Sakagami,: "Structure and chromosomal locus of the mouse gene encoding a cerebellar Purkinje cell-specific helix-loop-helix factor HES-3." Biochemical and Biophysical Research Communications. 203. 594-601 (1994)

[文献書誌] Koichi Takebayashi,: "Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5:identification of the neural precursor cell-specific promoter element." The Journal of Biological Chemistry. 270. 1342-1349 (1995)

[文献書誌] Chihiro Akazawa,: "A mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal is a positive transcriptional regulator expressed in the developing nervous system." The Journal of Biological Chemistry. (in press). (1995)

[文献書誌] Chikara Shimizu,: "MATH-2,a mammalian helix-loop-helix factor structurally related to the product of Drosophila proneural gene atonal,is specifically expressed in the nervous system." European Journal of Biochemistry. (in press). (1995)

[文献書誌] Takahashi,M.,: "Molecular heterogeneity among primary motoneurons and within myotomes revealed by the differential mRNA expression of novel Islet-1 homologs in embryonic zebrafish." (submitted to Dev.Biol.).

[文献書誌] Inoue,A.,: "Developmental regulation of Isl-1 mRNA expression during neuronal differentation in embryonic zebrafish." Devel.Dynamics,. 197. 1-11 (1994)

[文献書誌] 岡本仁: "脳の形成はいかに遺伝子によって決定され、制御されているか(特集 神経科学の謎)" 生体の科学. 46. 2-7 (1995)

[文献書誌] 岡本仁: "ゼブラフィッシュ神経細胞の運命決定," プレインメヂカル,. 6. 135-142 (1994)

[文献書誌] 岡本仁: "ゼブラフィッシュの脊髄と運動神経細胞の分化〜転写因子と組織間相互作用の役割〜" 生体の科学. 45. 182-188 (1994)

[文献書誌] 竹内隆: "IRESを用いたマウス遺伝子トラップ法による神経管形成機構の解析" 組織培養. 21. 55-59 (1995)

[文献書誌] J.Cooke: "Experimental control of axial pattern in the chick blastoderm by localexpression of Wnt and activin:The role of HNK-1 positive cells." Developmental Biology. 164. 513-527 (1994)

[文献書誌] Shinji Takada: "Wnt-3a regulates somite andtailbud formation in the mouse embryo." Genes & Development. 8. 174-189 (1994)

[文献書誌] 相沢慎一: "バイオマニュアルシリーズ8 羊土社" ジーンターゲテイング:ES細胞を用いた変異マウスの作製, 174 (1994)

[文献書誌] 松居靖久: "最新・細胞内シグナル伝達ライブラリー(山本雅編)" 羊土社(印刷中), (1995)

[文献書誌] 松居靖久: "生殖細胞-基礎から最前線まで(岡田益吉・長濱嘉孝編)" 共立出版(印刷中), (1995)

[文献書誌] Okano,H.,: "Drosophila visual system as a model to study neurobiology in gencral:identification of genes regulating cell fate decision in ncural development.Basic Neuroscience in Invertebrate." Business Center for Academic Societies Japan,Ed II.Koike.(印刷中), (1995)

[文献書誌] Mikoshiba,K.,: "Molecular genetic analyses of the myelin deficiency and cerebellar ataxia in“Progress in Brain Research"" Elsevier(印刷中), (1995)

[文献書誌] 岡野栄之: "“モデル生物系を用いた中枢神経系の発生と分化の解析"脳機能の解明に向けて" 赤池紀扶ら編、創風社(印刷中), (1995)

1994 年度研究成果報告書概要

竹市雅俊京都大学, 理学部, 教授 (00025454)

[文献書誌] 岡本仁: "脳の形成はいかに遺伝子によって決定され、制御されているか(特集神経科学の謎)" 生体の科学. 46. 2-7 (1995)