1998 年度実績報告書

雌雄器官の分化の解析

研究課題

研究課題/領域番号	07281101
研究種目	特定領域研究(A)
研究機関	北海道大学
研究代表者	米田好文北海道大学, 大学院理学研究科, 教授 (10124215)
研究分担者	三上哲夫北海道大学, 農学部, 教授 (50133715) 鳥山欽哉東北大学, 大学院農学研究科, 助教授 (20183882) 佐藤忍筑波大学, 生物科学系, 助教授 (70196236) 長戸康郎東京大学, 大学院農学生命科学研究科, 教授 (10143413) 石黒澄衛京都大学, 大学院理学研究科, 助手 (50260039)
キーワード	花序形態形成 / 花粉 / Y染色体 / 花分裂組織 / 雄性不稔性 / 脱分化 / ゼニゴケ / ユリ花粉母細胞
研究概要	本研究では、植物の生殖システム解明のために、雌雄器官の分化の解析を行った。まず、性染色体を持つアサ・ゼニゴケに注目し、DNA構造・機能レベルでの解明した。次に、単子葉・双子葉植物の花器官形成の遺伝子作用ネットワーク、調節機構、相互作用を遺伝子レベルで解明した。花器官の特に花粉の研究に重点を置き、雄性不稔性・花粉成熟遺伝子発現・未成熟花粉の脱分化の遺伝子支配機構の解明を行った。研究計画達成のために、各自発展させた成果を持ちより、総合的に研究討論・総括して本研究課題を遂行した。 1) 花序形態形成に関わるPDF1遺伝子を詳細に検討した。 2) dad1,mam遺伝子を解析し、花粉の乾燥耐性・花粉管ガイダンスを解析した。 3) イネの、穂の形・花分裂組織・器官数の制御に関わる突然変異体を解析した。 4) アサY染色体は、レトロトランスポゾンに富んだ特殊な配列であることを示した。 5) 葯特異的カルシウム結合タンパク質、花粉表面タンパク質、遺伝子を解析した。 6) テンサイの雄性不稔性の原因と考えられる遺伝子を解析した。 7) タバコ葯の脱分化時の発現遺伝子の詳細な解析を行った。 8) ゼニゴケY染色体の配列DNAを明らかにして、反復領域であることを示した。 9) テッポウユリの動原体タンパク質、ヒストン様タンパク質の遺伝子を解析した。 10) ユリ花粉母細胞の減数分裂特異的LIM遺伝子群の機能を推定した。以上のように、研究者間の研究成果を持ち寄り、雌雄器官分化の全体像に肉薄した。

研究成果
(33件)

すべてその他

すべて文献書誌 (33件)

[文献書誌] Yokoyama,R.: "The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia." The Plant Journal. 15・3. 301-310 (1998)
[文献書誌] Komeda,Y.: "Development of Inflorescences in Arabidopsis thaliana." Journal of Plant Research (JPR Symposium). 111・1102. 283-288 (1998)
[文献書誌] Abe,M.: "Cloning and Characterization of an L1 Layer-specific Gene in Arabidopsis thaliana." Plant and Cell Physiology. (in Press). (1999)
[文献書誌] Okada,K.: "Regulatory system of root patterning." J.Plant Res.111. 315-322 (1998)
[文献書誌] Takahashi,M.: "Panicle phytomer 1 mutations affect the panicle architecture of rice." Theor.Appl.Genet.96. 1050-1056 (1998)
[文献書誌] Itoh,J.-I.: "A recessive heterochronic mutation, plastochron 1, shortens the plastochron and elongates the vegetative phase in rice." Plant Cell. 10. 1511-1521 (1998)
[文献書誌] Nagato,Y.: "Morphogenetic Mutants in Rice." “Moleclar Biology of Rice", Springer-Verlag, in Tokyo. 79-99 (1998)
[文献書誌] Yamaguchi,T.: "Construction of the BAC contig encompassing the dI locus." Rice Genet.Newslett.15. in press (1998)
[文献書誌] 坂本浩一: "高等植物の性決定と性発現." 蛋白質核酸酵素. 43(4). 609-615 (1998)
[文献書誌] Sakamoto,K.: "Characterization ; genome sizes and morphology of sex chromosomes in hemp(Cannabis sativa L)." Cytologia. 63. 459-464 (1998)
[文献書誌] Toriyama,K.: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa." FEBS Letters. 424. 234-238 (1998)
[文献書誌] Sasaki,Y.: "Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus." Sex Plant Reprod. 11. 245-250 (1998)
[文献書誌] Okada,T.: "Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1,for immunotherapy." FEBS letters. 434. 255-260 (1998)
[文献書誌] Kitashiba,H.: "Partial male sterility in transgenic tobacco carrying an antisense gene for alternative oxidase under the control of a tapetum-specific promoter." Molecular Breeding. (in press). (1999)
[文献書誌] Estiati,A.: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantarum. 102. 325-327 (1998)
[文献書誌] Senda,M.: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. 128. 127-132 (1998)
[文献書誌] Senda,M.: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. 96. 964-968 (1998)
[文献書誌] Koike,T.: "Mitochondrial DNA variation follows a geographic pattern in Japanese beech species." Botanica Acta. 111. 87-91 (1998)
[文献書誌] Kanazawa,A.: "Small interspersed sequences that serve as recombination sites at the cox2 and atp6 loci in the mitochondrial genome of soybean are widely distributed in higher plants." Current Genetics. 33. 188-198 (1998)
[文献書誌] Tozuka,A.: "Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA." Thoeretical and Applied Genetics. 96. 170-176 (1998)
[文献書誌] Yamashita,S.: "Tam3 in Antirrhinum majus is exceptional transposon in resistant to alteration by abortive gap repair : identification of nested transposons." Molecular and General Genetics. 259. 468-474 (1998)
[文献書誌] Kato,S.: "Evolutionary changes in the structures of the cox2 and atp6 loci in the mitochondrial genome of soybean involving recombination across small interspersed sequences." Current Genetics. 34. 303-312 (1998)
[文献書誌] Kishima,Y.: "Structural conservation of the transposon Tam3 family in Antirrhinum majus and estimation of the number of copies able to transpose." Plant Molecular Biology. (in press). (1999)
[文献書誌] Onodera,Y.: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet." Journal of Plant Physiology. (in press). (1999)
[文献書誌] Nishiyama,R.: "Two mRNA species encoding calcium-dependent protein kinases are differentially expressed in sexual organs of Marchantica polymorpha through alternative splicing." Plant and Cell Physiology. 40(2). 205-212 (1998)
[文献書誌] Nagai,J.: "Expressed sequence tags from immature female sexual organ of a liverwort, Merchantia polymorpha." DNA Research. in press. (1999)
[文献書誌] Tanaka,I.: "The developmental fate of angiosperm pollen is associated with a preferential decrease in the level of histone H1 in the vegetative nucleus." Planta. 206. 561-569 (1998)
[文献書誌] Suzuki,T.: "Attachment of kinetochores to spindle microtubules during meiosis I of Lilium microsporocytes." Chromosome Res.(in press). (1999)
[文献書誌] Tanaka,I.: "A novel histone variant localized in nucleoli of higher plant cells." Chromosoma. (in press). (1990)
[文献書誌] Mousavi,A.: "A novel glycine-rich protein is associated with starch grain accumlation during anther development." Plant Cell Physiol.40 (in press). (1999)
[文献書誌] Ogata,S.: "Nuclear localization signal of protein which accumulate specifically during meiosis lily microsporecytes." Plant Cell Rep.(in press). (1999)
[文献書誌] 田中一朗: "クロマチンの構造と機能(福井・芦川編)Universal Academy Press" クロモソーム-植物染色体情報の解析と利用-, 12 (1998)
[文献書誌] 田中一朗: "新生物学ライブラリ・サイエンス社" 遺伝学, in press (1999)

1998 年度 実績報告書

雌雄器官の分化の解析

研究代表者

米田 好文 北海道大学, 大学院理学研究科, 教授 (10124215)

研究成果

[文献書誌] Yokoyama,R.: "The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia." The Plant Journal. 15・3. 301-310 (1998)

[文献書誌] Komeda,Y.: "Development of Inflorescences in Arabidopsis thaliana." Journal of Plant Research (JPR Symposium). 111・1102. 283-288 (1998)

[文献書誌] Abe,M.: "Cloning and Characterization of an L1 Layer-specific Gene in Arabidopsis thaliana." Plant and Cell Physiology. (in Press). (1999)

[文献書誌] Okada,K.: "Regulatory system of root patterning." J.Plant Res.111. 315-322 (1998)

[文献書誌] Takahashi,M.: "Panicle phytomer 1 mutations affect the panicle architecture of rice." Theor.Appl.Genet.96. 1050-1056 (1998)

[文献書誌] Itoh,J.-I.: "A recessive heterochronic mutation, plastochron 1, shortens the plastochron and elongates the vegetative phase in rice." Plant Cell. 10. 1511-1521 (1998)

[文献書誌] Nagato,Y.: "Morphogenetic Mutants in Rice." “Moleclar Biology of Rice", Springer-Verlag, in Tokyo. 79-99 (1998)

[文献書誌] Yamaguchi,T.: "Construction of the BAC contig encompassing the dI locus." Rice Genet.Newslett.15. in press (1998)

[文献書誌] 坂本 浩一: "高等植物の性決定と性発現." 蛋白質核酸酵素. 43(4). 609-615 (1998)

[文献書誌] Sakamoto,K.: "Characterization ; genome sizes and morphology of sex chromosomes in hemp(Cannabis sativa L)." Cytologia. 63. 459-464 (1998)

[文献書誌] Toriyama,K.: "Molecular cloning of a cDNA encoding a pollen extracellular protein as a potential source of a pollen allergen in Brassica rapa." FEBS Letters. 424. 234-238 (1998)

[文献書誌] Sasaki,Y.: "Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus." Sex Plant Reprod. 11. 245-250 (1998)

[文献書誌] Okada,T.: "Engineering of hypoallergenic mutants of the Brassica pollen allergen, Bra r 1,for immunotherapy." FEBS letters. 434. 255-260 (1998)

[文献書誌] Kitashiba,H.: "Partial male sterility in transgenic tobacco carrying an antisense gene for alternative oxidase under the control of a tapetum-specific promoter." Molecular Breeding. (in press). (1999)

[文献書誌] Estiati,A.: "The ribosomal protein S7 gene is transcribed and edited in sugar beet mitochondria." Physiologia Plantarum. 102. 325-327 (1998)

[文献書誌] Senda,M.: "Cytoplasmic diversity in leaf beet cultivars as revealed by mitochondrial DNA analysis." Hereditas. 128. 127-132 (1998)

[文献書誌] Senda,M.: "Recombination events across the atpA-associated repeated sequences in the mitochondrial genomes of beets." Theoretical and Applied Genetics. 96. 964-968 (1998)

[文献書誌] Koike,T.: "Mitochondrial DNA variation follows a geographic pattern in Japanese beech species." Botanica Acta. 111. 87-91 (1998)

[文献書誌] Kanazawa,A.: "Small interspersed sequences that serve as recombination sites at the cox2 and atp6 loci in the mitochondrial genome of soybean are widely distributed in higher plants." Current Genetics. 33. 188-198 (1998)

[文献書誌] Tozuka,A.: "Composite and clinal distribution of Glycine soja in Japan revealed by RFLP analysis of mitochondrial DNA." Thoeretical and Applied Genetics. 96. 170-176 (1998)

[文献書誌] Yamashita,S.: "Tam3 in Antirrhinum majus is exceptional transposon in resistant to alteration by abortive gap repair : identification of nested transposons." Molecular and General Genetics. 259. 468-474 (1998)

[文献書誌] Kato,S.: "Evolutionary changes in the structures of the cox2 and atp6 loci in the mitochondrial genome of soybean involving recombination across small interspersed sequences." Current Genetics. 34. 303-312 (1998)

[文献書誌] Kishima,Y.: "Structural conservation of the transposon Tam3 family in Antirrhinum majus and estimation of the number of copies able to transpose." Plant Molecular Biology. (in press). (1999)

[文献書誌] Onodera,Y.: "Heterogeneity of the atp6 presequences in normal and different sources of male-sterile cytoplasms of sugar beet." Journal of Plant Physiology. (in press). (1999)

[文献書誌] Nishiyama,R.: "Two mRNA species encoding calcium-dependent protein kinases are differentially expressed in sexual organs of Marchantica polymorpha through alternative splicing." Plant and Cell Physiology. 40(2). 205-212 (1998)

[文献書誌] Nagai,J.: "Expressed sequence tags from immature female sexual organ of a liverwort, Merchantia polymorpha." DNA Research. in press. (1999)

[文献書誌] Tanaka,I.: "The developmental fate of angiosperm pollen is associated with a preferential decrease in the level of histone H1 in the vegetative nucleus." Planta. 206. 561-569 (1998)

[文献書誌] Suzuki,T.: "Attachment of kinetochores to spindle microtubules during meiosis I of Lilium microsporocytes." Chromosome Res.(in press). (1999)

[文献書誌] Tanaka,I.: "A novel histone variant localized in nucleoli of higher plant cells." Chromosoma. (in press). (1990)

[文献書誌] Mousavi,A.: "A novel glycine-rich protein is associated with starch grain accumlation during anther development." Plant Cell Physiol.40 (in press). (1999)

[文献書誌] Ogata,S.: "Nuclear localization signal of protein which accumulate specifically during meiosis lily microsporecytes." Plant Cell Rep.(in press). (1999)

[文献書誌] 田中 一朗: "クロマチンの構造と機能(福井・芦川編)Universal Academy Press" クロモソーム-植物染色体情報の解析と利用-, 12 (1998)

[文献書誌] 田中 一朗: "新生物学ライブラリ・サイエンス社" 遺伝学, in press (1999)

1998 年度実績報告書

米田好文北海道大学, 大学院理学研究科, 教授 (10124215)

[文献書誌] 坂本浩一: "高等植物の性決定と性発現." 蛋白質核酸酵素. 43(4). 609-615 (1998)

[文献書誌] 田中一朗: "クロマチンの構造と機能(福井・芦川編)Universal Academy Press" クロモソーム-植物染色体情報の解析と利用-, 12 (1998)

[文献書誌] 田中一朗: "新生物学ライブラリ・サイエンス社" 遺伝学, in press (1999)