1995 年度実績報告書

新たに見いだされた神経特異的カルシウム結合蛋白質の構造と機能

研究課題

研究課題/領域番号	07309013
研究種目	総合研究(A)
研究機関	大阪大学
研究代表者	河村悟大阪大学, 理学部, 教授 (80138122)
研究分担者	日高弘義名古屋大学, 医学部, 教授 (80100171) 三木直正大阪大学, 医学部, 教授 (40094445) 徳永史生大阪大学, 理学部, 教授 (80025452) 高松研東邦大学, 医学部, 教授 (90154898) 久野高義神戸大学, 医学部, 教授 (50144564)
キーワード	カルシウム結合蛋白質 / 神経 / ヒポカルシン / S-モジュリン / ニューロカルシン / カルモジュリン / リカバリン / NVP
研究概要	平成7年度には以下の成果が得られた。 1.NCaP遺伝子のクローニング分裂酵母モデル系において、 NVP 様蛋白質を新たに精製し、遺伝子のクローニングを行いつつある(久野)。また、既にクローニングの終わっているヒポカルシンについて、ゲノム DNA の解析を行った(高松)。その結果、ゲノム構造はリカバリンのものと同様であることがわかった。さらに、NCaP の分子系統樹を作成し、NCaP の分子進化を明らかにした(徳永)。2.NCaP の大量調製 S-モジュリンとs26の大量発現系を確立し、以後の実験に使用することができるようになった(徳永)。3.NCaP 結合蛋白質の同定 S-モジュリン(河村)、ニューロカルシン(日高)について、蛋白化学的方法によって結合蛋白質の同定を行った。その結果、S-モジュリンについてはロドプシンキナーゼであることが分かった。4.NCaP の局在 S-モジュリン、s26について、網膜内での局在(河村)、ニューロカルシンについて中枢神経系での局在(日高)を明らかにした。5.NCaP の構造と機能の関係リカバリンの脂質修飾によってロドプシンキナーゼとの相互作用が高まることが明らかになった。(深田)。NVP のmRNA 量が減少していることから、分裂病における NVP の関与が示唆された(久野)。6.他の系との比較キメラカルモジュリンを使って、カルモジュリンの構造と機能の関係について検討した(矢沢)。また、GRK2 系において、カルモジュリンが受容体のリン酸化を阻害することが分かった。また、これにはトランスデューシンの関与はなかった(芳賀)。MEKA 蛋白質のトランスデューシンとの結合部位を明らかにした(三木)。嗅覚系における NCaP の関与を検討する目的で、嗅上皮におけるS-モジュリン様蛋白質を検索し、p26olf を単離・精製し、クローニングを行った(河村)。

研究成果

(29件)

すべてその他

すべて文献書誌 (29件)

[文献書誌] De Castro,E.: "Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors." Biochem.Biophys.Res.Commun.216. 133-140 (1995)
[文献書誌] 河村悟: "視覚中枢の分子機構-網膜-" 分子神経科学の最先端. 135-145 (1995)
[文献書誌] Kawamura,S.: "Unsolved issues in S-modulin /vecoverin study." Behav.Brain Sci.18. 479-480 (1995)
[文献書誌] Kajimoto,Y.: "Delayed changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidineadministration." J.Neural Transm.100. 257-262 (1995)
[文献書誌] Cmay,S.B.: "1α,25-dihydroxyvitamin D3-induced upregulation of calcineurin during leukemic HL-60 cell differentiation." Blood. 87(in press). (1996)
[文献書誌] Kuno,T.: "Purification and activation of cyclic GMP-dependent protein Kinase." Methods in molecular biology. 41. 123-127 (1995)
[文献書誌] Bastianelli,E.: "Hippocalcin in rat retina.Comparison with calbindin-D28K,calvetinin and neurocalcin." Experimental Eye Research. 60. 257-266 (1995)
[文献書誌] Takamatsu,K.: "A novel protein family of nenronal modulators." Behavioral and Brain Sciences. 18. 489-490 (1995)
[文献書誌] Saitoh,S.: "The development of neural visinin-like Ca^<2+>-binding protein Zimmunoreactivity in the rat neocortex and hippocampus." Neuroscience Research. 23. 383-388 (1995)
[文献書誌] Seiya Kayada: "Cloning and Expression of Frog Rhodopsin c DNA" Comparative Biochemistry and Physiology. 110B. 599-604 (1995)
[文献書誌] Osamu Hisatomi: "Molecular Cloning and Characterization of the Putative Ultraviolet-Sensitive Visual" Vision Research. 20(in press). (1995)
[文献書誌] 徳永史生: "動物の色覚" 科学. 65. 485-492 (1995)
[文献書誌] Kuo,c-H.: "Determination of a necdin cis-acting element required for neuron specific expression by using zebra fish." Biochem.Biophys.Res.Comm.211. 438-446 (1995)
[文献書誌] Taira,E.: "Expression and functional analysis of a novel isoform of gicerin,an immunoglobulin superfamily cell adhesion molecule." J.Biol.Chem.270. 28681-28687 (1995)
[文献書誌] Ikemoto,M.: "Decrease in CRE-binding activity by chronic morphine administration in mouse brain." Neuro Report. 6. 262-264 (1995)
[文献書誌] K.Okazaki.: "Siooβ is a target protein of neurocalcin δ,an isoform abundant in glial cells." Biochemical Journal. 306. 551-555 (1995)
[文献書誌] T.Junttila.: "Localization of neurocalcin-like immunoreactivity in rat cranial motoneurons and spinal cord interneurons." Neuroscience Letters. 183. 100-103 (1995)
[文献書誌] B.C.M.Potts: "The structure of calcyclin reveals a novel homodimeric fold for S100 Ca^<2+>-binding proteins." Natute Structural Biology 2. 790-796 (1995)
[文献書誌] Nakamura,F.: "Characterization of G9 family G proteins,GL1alpha(G14alpha),GL2alpha(G11alpha),and G9alpha expressed in the baculovirus-insect cell system." J.Biol.Chem.270. 6246-6253 (1995)
[文献書誌] Shui,Z.: "Receptor kinase-dependent desensitization of the muscarinic K^+ current in rat atrial cells." J.Physiol.(Lond.). 487. 359-366 (1995)
[文献書誌] Haga,K.: "Phosphorylation of human m1 muscarinic acetylcholine receptors by G proteincoupled receptor kinase 2 and protein kinase C." J.Biol.Chem.271. 2776-2782 (1996)
[文献書誌] Kamon Sanada: "Unique lipids and unique properties of retinal proteins." Behavioral and Brain Sciences. 18. 486-487 (1995)
[文献書誌] Kamon Sanada: "Role of heterogeneous N-terminal acylation of recoverin in rhodopsin phosphorylation." J.Biol.Chem.270. 15459-15462 (1995)
[文献書誌] Osamu Shono: "Preparation and characterization of monoclonal antibodies specific for lauroylated isoform of bovine transducin α-subunit." J.Neurochem. (in press). (1996)
[文献書誌] T.Katoh: "Evidence for Involvement of a 12-Residue Peptide Segment of the Heavy Chain in the Neck Region of Smooth Muscle Myosin in Formation of the 10S Conformation" J.Biochem.118. 428-434 (1995)
[文献書誌] M.Moriya: "Immunohistochemical localization of the calcium/calmodulin-dependentprotein phosphatase,calcineurin,in the mouse testis:its unique accumulationin spermatid nuclei." Cell Tissue Res.281. 273-281 (1995)
[文献書誌] H.Yoshino: "Solution X-ray Scattering Data Show Structural Differences between Yeast and Vertebrate Calmodulin:Implications for Structure/Function." Biochemistry. 35(in press).
[文献書誌] Kawamura,S.: "Neurobiology of the outen retina,(分担執筆)" Chapman & Hall,London., 501(105-131) (1995)
[文献書誌] Mukai,H.: "Guidebook to Molecular Chaperones and Protein Folding Catalysts" Oxford Univ.Press(in press), (1996)

1995 年度 実績報告書

新たに見いだされた神経特異的カルシウム結合蛋白質の構造と機能

研究代表者

河村 悟 大阪大学, 理学部, 教授 (80138122)

研究成果

[文献書誌] De Castro,E.: "Regulation of rhodopsin phosphorylation by a family of neuronal calcium sensors." Biochem.Biophys.Res.Commun.216. 133-140 (1995)

[文献書誌] 河村 悟: "視覚中枢の分子機構-網膜-" 分子神経科学の最先端. 135-145 (1995)

[文献書誌] Kawamura,S.: "Unsolved issues in S-modulin /vecoverin study." Behav.Brain Sci.18. 479-480 (1995)

[文献書誌] Kajimoto,Y.: "Delayed changes in neural visinin-like calcium-binding protein gene expression caused by acute phencyclidineadministration." J.Neural Transm.100. 257-262 (1995)

[文献書誌] Cmay,S.B.: "1α,25-dihydroxyvitamin D3-induced upregulation of calcineurin during leukemic HL-60 cell differentiation." Blood. 87(in press). (1996)

[文献書誌] Kuno,T.: "Purification and activation of cyclic GMP-dependent protein Kinase." Methods in molecular biology. 41. 123-127 (1995)

[文献書誌] Bastianelli,E.: "Hippocalcin in rat retina.Comparison with calbindin-D28K,calvetinin and neurocalcin." Experimental Eye Research. 60. 257-266 (1995)

[文献書誌] Takamatsu,K.: "A novel protein family of nenronal modulators." Behavioral and Brain Sciences. 18. 489-490 (1995)

[文献書誌] Saitoh,S.: "The development of neural visinin-like Ca^<2+>-binding protein Zimmunoreactivity in the rat neocortex and hippocampus." Neuroscience Research. 23. 383-388 (1995)

[文献書誌] Seiya Kayada: "Cloning and Expression of Frog Rhodopsin c DNA" Comparative Biochemistry and Physiology. 110B. 599-604 (1995)

[文献書誌] Osamu Hisatomi: "Molecular Cloning and Characterization of the Putative Ultraviolet-Sensitive Visual" Vision Research. 20(in press). (1995)

[文献書誌] 徳永史生: "動物の色覚" 科学. 65. 485-492 (1995)

[文献書誌] Kuo,c-H.: "Determination of a necdin cis-acting element required for neuron specific expression by using zebra fish." Biochem.Biophys.Res.Comm.211. 438-446 (1995)

[文献書誌] Taira,E.: "Expression and functional analysis of a novel isoform of gicerin,an immunoglobulin superfamily cell adhesion molecule." J.Biol.Chem.270. 28681-28687 (1995)

[文献書誌] Ikemoto,M.: "Decrease in CRE-binding activity by chronic morphine administration in mouse brain." Neuro Report. 6. 262-264 (1995)

[文献書誌] K.Okazaki.: "Siooβ is a target protein of neurocalcin δ,an isoform abundant in glial cells." Biochemical Journal. 306. 551-555 (1995)

[文献書誌] T.Junttila.: "Localization of neurocalcin-like immunoreactivity in rat cranial motoneurons and spinal cord interneurons." Neuroscience Letters. 183. 100-103 (1995)

[文献書誌] B.C.M.Potts: "The structure of calcyclin reveals a novel homodimeric fold for S100 Ca^<2+>-binding proteins." Natute Structural Biology 2. 790-796 (1995)

[文献書誌] Nakamura,F.: "Characterization of G9 family G proteins,GL1alpha(G14alpha),GL2alpha(G11alpha),and G9alpha expressed in the baculovirus-insect cell system." J.Biol.Chem.270. 6246-6253 (1995)

[文献書誌] Shui,Z.: "Receptor kinase-dependent desensitization of the muscarinic K^+ current in rat atrial cells." J.Physiol.(Lond.). 487. 359-366 (1995)

[文献書誌] Haga,K.: "Phosphorylation of human m1 muscarinic acetylcholine receptors by G proteincoupled receptor kinase 2 and protein kinase C." J.Biol.Chem.271. 2776-2782 (1996)

[文献書誌] Kamon Sanada: "Unique lipids and unique properties of retinal proteins." Behavioral and Brain Sciences. 18. 486-487 (1995)

[文献書誌] Kamon Sanada: "Role of heterogeneous N-terminal acylation of recoverin in rhodopsin phosphorylation." J.Biol.Chem.270. 15459-15462 (1995)

[文献書誌] Osamu Shono: "Preparation and characterization of monoclonal antibodies specific for lauroylated isoform of bovine transducin α-subunit." J.Neurochem. (in press). (1996)

[文献書誌] T.Katoh: "Evidence for Involvement of a 12-Residue Peptide Segment of the Heavy Chain in the Neck Region of Smooth Muscle Myosin in Formation of the 10S Conformation" J.Biochem.118. 428-434 (1995)

[文献書誌] M.Moriya: "Immunohistochemical localization of the calcium/calmodulin-dependentprotein phosphatase,calcineurin,in the mouse testis:its unique accumulationin spermatid nuclei." Cell Tissue Res.281. 273-281 (1995)

[文献書誌] H.Yoshino: "Solution X-ray Scattering Data Show Structural Differences between Yeast and Vertebrate Calmodulin:Implications for Structure/Function." Biochemistry. 35(in press).

[文献書誌] Kawamura,S.: "Neurobiology of the outen retina,(分担執筆)" Chapman & Hall,London., 501(105-131) (1995)

[文献書誌] Mukai,H.: "Guidebook to Molecular Chaperones and Protein Folding Catalysts" Oxford Univ.Press(in press), (1996)

1995 年度実績報告書

河村悟大阪大学, 理学部, 教授 (80138122)

[文献書誌] 河村悟: "視覚中枢の分子機構-網膜-" 分子神経科学の最先端. 135-145 (1995)