1995 年度実績報告書

ヒトT細胞白血病ウイルスI型のシェ-グレン症候群発症機序解明と治療法に関する研究

研究課題

研究課題/領域番号	07670534
研究種目	一般研究(C)
研究機関	長崎大学
研究代表者	江口勝美長崎大学, 医学部, 助教授 (30128160)
研究分担者	塚田敏昭長崎大学, 医学部, 医員河部庸次郎長崎大学, 医学部, 助手 (50244059)
キーワード	ヒトT細胞白血病ウイルスI型 / シェ-グレン症候群 / Tax遺伝子 / 抗HTLV-I抗体 / 自己抗体 / アポトーシス / HTLV-I関連脊髄症 / T細胞レセプター
研究概要	A-I. HTLV-I感染によるSjogren症候群(SjS)発症機序の解明 (1)唾液腺組織におけるHTLV-IプロウイルスDNAの局在を見る。淡白レベルでは検出できなかった。In situ PCR法で検討中である。 (2)唾液腺組織でのHTLV-IプロウイルスDNAの検出。 PCRによりLTR,gag,pol,env,pXの各領域のプロウイルスDNAを検出した。 a)抗HTLV-I抗体陽性SjS患者7例では全例に末梢血リンパ球も小唾液腺組織でもLTR,gag,pol,env,pXのすべての領域が検出できた。 b)抗HTLV-I抗体陰性SjS患者では9例中1例にのみ小唾液腺組織でnested PCRでHTLV-IpX領域のみが検出できた。 pX領域のDNAsequenceは報告されているHTLV-Iのsequenceと99%一致していた。ウイルス量は抗HTLV-I抗体陽性者の小唾液腺組織のウイルス量に比較して低い値を示した。 A-II. HTLV-I関連脊髄症(HAM)患者のSjogren症候群の合併 (1)HAM患者(10例)を無作為に小唾液腺生検を行うと単核球の浸潤が全症例に認められた。 (2)SjSのヨーロッパ診断基準に照らし合わせると6例がdefinite SjS、2例がprobable SjS、2例がnegative SjSであり、高率にSjSを合併していた。 (3)自己抗体出現は抗核抗体、抗SS-A抗体、リウマトイド因子で低値を示した。 (4)浸潤している細胞は主としてCD4^+細胞であった。 A-III. 抗HTLV-I抗体感染SjS患者と非感染SjS患者の比較 (1)臨床的には感染群でHTLV-I関連疾患の合併が多いという特徴があるが、臨床症状や自己抗体の出現率に両群で差を認めなかった。 (2)SjSの小唾液腺組織に浸潤しているリンパ球は両群とも主にCD4^+細胞であった。 A-IV. 抗HTLV-I抗体陽性SjS患者の小唾液腺浸潤リンパ球性質 (1)浸潤しているリンパ球はCD4^+細胞が主で、一部CD8^+細胞やB細胞が浸潤していた。 (2)Fas抗原の発現が腺管細胞に、Fasリガンドが浸潤リンパ球に発現し、nick end labelling法でアポトーシスが検出された。 (3)T細胞レセプターの解析について現在検討中である。

研究成果

(23件)

すべてその他

すべて文献書誌 (23件)

[文献書誌] Tomoki Origuchi,et al.: "Increased livels of serum IgM antibody to staphylococcal enterotoxin B in patients with rheumatoid arthritis" Ann Rheum Dis. 54. 744-747 (1995)
[文献書誌] Hiroyuki Uno,et al: "Removal of LDL from plasma by adsorption reduces adhesion molecules on mononuclear cells in patients with arteriosclerotic obliteranc" Atherosclerosis. 116. 93-102 (1995)
[文献書誌] Kiyoshi Migita,et al: "The role of protein kinase in human synovial fibroblast growth" Biochem Biophs Res Commun. 210(3). 1066-1075 (1995)
[文献書誌] Toshiaki Tsukada,et al: "Transform growth factor β1 induces apoptotic cell death in cultured human umbilical vein endothelial cells with down-regulated expression on bc1-2" Biochem Biophs Res Commun. 210(3). 1076-1082 (1995)
[文献書誌] Tomoki Origuchi,et al: "Synovial cells are potent antigen-presenting cells for superantigens,staphylococcal enterotoxin B" Clinical and Experimental Immunology. 99(3). 345-351 (1995)
[文献書誌] Kiyoshi Migita,et al: "Thyrosine phosphorylation participates in peripheral T-cell activation and programmed cell death in vivo" Immunology. 85(4). 550-555 (1995)
[文献書誌] Kiyoshi Migita,et al: "FK506 augments activation-induced programmed cell death of T lymphocytes in vivo" J Clin Invest. 96(2). 727-732 (1995)
[文献書誌] Kiyoshi Migita,et al: "Defects of T cell receptor-mediated signaling in anergic T cells" J Immunol. 155. 5083-5087 (1995)
[文献書誌] Souko Sakito,et al: "Serum cytokines in patients with rheumatoid arthritis : correlation of interferon γ and tumor necrosis factor α with the characteristics of peripheral blood mononuclear cells" Rheumatology International. 53(1). 31-37 (1995)
[文献書誌] Masahiko Tsuboi,et al: "Fas antigen expression on synovial cells was down-regulated by interleukin 1β" Biochem Biophs Res Comun. 218(1). 280-285 (1996)
[文献書誌] Naofumi Ishikawa,et al: "Defective organification of iodide causing congenital goitrous hypothyroidism" J Clin Endocrinol Metab. 81(1). 376-383 (1996)
[文献書誌] Katsumi Eguchi,et al: "High seroprevalence of anti-HTLV-I antibody in rheumatoid arthritis" Arthritis Rheum. (in press). (1996)
[文献書誌] Koji Yano,et al: "Behcet's disease complicated with myelodysplastic syndrome : a report of two cases and review of literature" Clinical Rheumatology. (in press). (1996)
[文献書誌] Yukitaka Ueki,et al: "Increased nitric oxide levels in patients with rheumatoid arthritis" J Rheumatol. (in press). (1996)
[文献書誌] Kiyoshi Migita,et al: "Defects of T cell receptor-mediated signaling in anergic T cells" J Immunol. (in press). (1996)
[文献書誌] Satsohi Yamasaki,et al: "Wegener's granulomatosis overlapped with Takayasu arteritis" Clin Rheumatol. (accepted). (1996)
[文献書誌] Masahiro Izumi,et al: "MR imaging of the parotid and lacrimal glands in Sjogrens syndrome : a proposal for new diagnostic criteria" Am J Roentgenol. (accepted). (1996)
[文献書誌] Yoshiko Arichi,et al: "Real-time texture analysis of ultrasonographic features of the parotid gland in Sjogren's syndrome" Am J Roentgenol. (accepted). (1996)
[文献書誌] Kiyoshi Migita,et al: "The effects of the immunosuppressant rapamycin on the growth of rheumatoid arthritis synovial cells" Clin Exp Immunol. (accepted). (1996)
[文献書誌] Naoki Matsuoka,et al: "Effects of clarithromycin on costimulatory molecule expression and cytokine production by synovial fibroblast like cells" Clin Exp Immunol. (accepted). (1996)
[文献書誌] Masako Kita,et al: "Staphylococcal enterofoxin B specific adhesion of murine splenic T cells to a human endothelial cell line" Immunology. (accepted). (1996)
[文献書誌] Hiroaki Ida,et al: "Proceeding of 5th International Conference on Human Differentiation Antigens" 2 (1995)
[文献書誌] Katsumi Eguchi,et al: "Bailliere's Clinical Endocrinology and Metabolism" 24 (1995)

1995 年度 実績報告書

ヒトT細胞白血病ウイルスI型のシェ-グレン症候群発症機序解明と治療法に関する研究

研究代表者

江口 勝美 長崎大学, 医学部, 助教授 (30128160)

研究成果

[文献書誌] Tomoki Origuchi,et al.: "Increased livels of serum IgM antibody to staphylococcal enterotoxin B in patients with rheumatoid arthritis" Ann Rheum Dis. 54. 744-747 (1995)

[文献書誌] Hiroyuki Uno,et al: "Removal of LDL from plasma by adsorption reduces adhesion molecules on mononuclear cells in patients with arteriosclerotic obliteranc" Atherosclerosis. 116. 93-102 (1995)

[文献書誌] Kiyoshi Migita,et al: "The role of protein kinase in human synovial fibroblast growth" Biochem Biophs Res Commun. 210(3). 1066-1075 (1995)

[文献書誌] Toshiaki Tsukada,et al: "Transform growth factor β1 induces apoptotic cell death in cultured human umbilical vein endothelial cells with down-regulated expression on bc1-2" Biochem Biophs Res Commun. 210(3). 1076-1082 (1995)

[文献書誌] Tomoki Origuchi,et al: "Synovial cells are potent antigen-presenting cells for superantigens,staphylococcal enterotoxin B" Clinical and Experimental Immunology. 99(3). 345-351 (1995)

[文献書誌] Kiyoshi Migita,et al: "Thyrosine phosphorylation participates in peripheral T-cell activation and programmed cell death in vivo" Immunology. 85(4). 550-555 (1995)

[文献書誌] Kiyoshi Migita,et al: "FK506 augments activation-induced programmed cell death of T lymphocytes in vivo" J Clin Invest. 96(2). 727-732 (1995)

[文献書誌] Kiyoshi Migita,et al: "Defects of T cell receptor-mediated signaling in anergic T cells" J Immunol. 155. 5083-5087 (1995)

[文献書誌] Souko Sakito,et al: "Serum cytokines in patients with rheumatoid arthritis : correlation of interferon γ and tumor necrosis factor α with the characteristics of peripheral blood mononuclear cells" Rheumatology International. 53(1). 31-37 (1995)

[文献書誌] Masahiko Tsuboi,et al: "Fas antigen expression on synovial cells was down-regulated by interleukin 1β" Biochem Biophs Res Comun. 218(1). 280-285 (1996)

[文献書誌] Naofumi Ishikawa,et al: "Defective organification of iodide causing congenital goitrous hypothyroidism" J Clin Endocrinol Metab. 81(1). 376-383 (1996)

[文献書誌] Katsumi Eguchi,et al: "High seroprevalence of anti-HTLV-I antibody in rheumatoid arthritis" Arthritis Rheum. (in press). (1996)

[文献書誌] Koji Yano,et al: "Behcet's disease complicated with myelodysplastic syndrome : a report of two cases and review of literature" Clinical Rheumatology. (in press). (1996)

[文献書誌] Yukitaka Ueki,et al: "Increased nitric oxide levels in patients with rheumatoid arthritis" J Rheumatol. (in press). (1996)

[文献書誌] Kiyoshi Migita,et al: "Defects of T cell receptor-mediated signaling in anergic T cells" J Immunol. (in press). (1996)

[文献書誌] Satsohi Yamasaki,et al: "Wegener's granulomatosis overlapped with Takayasu arteritis" Clin Rheumatol. (accepted). (1996)

[文献書誌] Masahiro Izumi,et al: "MR imaging of the parotid and lacrimal glands in Sjogrens syndrome : a proposal for new diagnostic criteria" Am J Roentgenol. (accepted). (1996)

[文献書誌] Yoshiko Arichi,et al: "Real-time texture analysis of ultrasonographic features of the parotid gland in Sjogren's syndrome" Am J Roentgenol. (accepted). (1996)

[文献書誌] Kiyoshi Migita,et al: "The effects of the immunosuppressant rapamycin on the growth of rheumatoid arthritis synovial cells" Clin Exp Immunol. (accepted). (1996)

[文献書誌] Naoki Matsuoka,et al: "Effects of clarithromycin on costimulatory molecule expression and cytokine production by synovial fibroblast like cells" Clin Exp Immunol. (accepted). (1996)

[文献書誌] Masako Kita,et al: "Staphylococcal enterofoxin B specific adhesion of murine splenic T cells to a human endothelial cell line" Immunology. (accepted). (1996)

[文献書誌] Hiroaki Ida,et al: "Proceeding of 5th International Conference on Human Differentiation Antigens" 2 (1995)

[文献書誌] Katsumi Eguchi,et al: "Bailliere's Clinical Endocrinology and Metabolism" 24 (1995)

1995 年度実績報告書

江口勝美長崎大学, 医学部, 助教授 (30128160)