ヒト・ゲノム解析研究

1995 年度 実績報告書

• 研究課題/領域番号: 07NP0201
• 研究機関: 大阪大学
• 研究代表者: 松原 謙一 大阪大学, 細胞生体工学センター, 教授 (20037394)
• 研究分担者: 大木 操 国立がんセンター研究所, 部長 (00158792) ; 中村 祐輔 東京大学, 医科学研究所, 教授 (70217909) ; 吉田 光昭 東京大学, 医科学研究所, 教授 (80012607) ; 吉川 寛 奈良先端科学技術大学院大学, 教授 (70019876) ; 榊 佳之 東京大学, 医科学研究所, 教授 (10112327)
• キーワード: ヒト・ゲノム / ゲノム構造解析 / ゲノム機能解析 / cDNA / DNA解析技術 / モデル生物 / 生物情報科学 / 塩基配列決定

研究概要

ヒトのからだの形成とはたらきのしくみの理解を深め人類の健康と幸福に役立つ知識と技術の生産に貢献するための研究を進める。また、生命の研究と情報科学の融合による研究の情報化と生命現象の解析の高速・大量化に対処するために本研究を発足させた。研究は生命の設計図であるゲノムの構造と機能を解析研究し、そこに包含される遺伝情報の理解を目指して進めた。このために総括班ならびに3つの研究班を組織して研究の推進を図り次の成果を得た。
1.総括班。全体としての活動を円滑に進めるため各種会合の企画立案調整、情報交流、調査などを積極的に進めた。また「ヒトゲノム解析と共役した遺伝病家系研究」、「日本における生物学的研究材料保存・供給体制のあり方について」「社会との接点の問題研究」について三つの推進小委員会を設けて調査研究、提言作成の作業を進めた。また、公開シンポジウム、国際シンポジウム講習会、ジャーナリストのためのチュートリアルとニュースレター発行等の広報活動も行った。
2.ヒト・ゲノム構造解析班。技術開発を進めつつ特定の染色体あるいは染色体の機能領域の構造解析研究を行った。とくにヒト染色体21番、11番につき物理地図の作製を進め、また、染色体3、6、8、11、X、Yにつき高密度のコスミドマップ作製に成果をあげた。これらの成果を活用して、数種の疾病遺伝子の発見と入手に成功した。特定領域として免疫グロブリン遺伝子H鎖V領域とHLA遺伝子群を選び詳細なマップ作りと遺伝子の同定を実行し、シーケンシング時代のモデルとなる先導的研究を進めた。
3.cDNA解析班。ヒトゲノムの機能解析のため、遺伝子として発現する部分に研究対象を絞り、ヒトの各種組織におけるcDNAの存在頻度情報の収集を進め、「3'特異的cDNAライブラリー」の構築を40余の組織について完成させた。また、200を越えるcDNAの全シーケンスの収集を達成した。これと共に、線虫をモデル系としてゲノムの構造解析と発生のステージで発現する遺伝子の情報を共役させる研究に進展をみた。
4.DNA解析技術開発班。巨大ゲノムを解析するための技術開発とモデル生物(分裂酵母と枯草菌)を用いる大規模シーケンシングの実行とその効率化に関する研究を進めた。また、マーカーDNAの染色体上へのマッピングに係わる技術開発に進歩を示すことができた。このほかに、ゲノムスキャンニング法の応用、T3ファージによるDNA欠失セットの作成技術の完成をはじめ、国際協力によって大量シーケンシングを実行する研究を進めて、ゲノムデータベース作成を開始した。

研究成果

• [文献書誌] Isobe, M.: "Assignment of the ets-related transcription factor E1A-F gene(ETV4) to human chromosome region 17q21" Genomics. 28. 357-359 (1995)
• [文献書誌] Narducci, M. G. (Isobe, M. ): "TCL1 oncogene activation in preleukemic T cells from a case of ataxia-telangiectasia" Blood. 86. 2358-2363 (1995)
• [文献書誌] Nagata, T. (Inoko, H. ): "Physical mapping of the retinoid X receptor b gene in mouse and man" Immunogenetics. 41. 74-82 (1995)
• [文献書誌] Fukagawa, T. (Inoko, H. ): "A boundary of long-range G+C% mosaic domains in the human MHC locus: Pseudoautosomal boundary-like sequence exists near the boundary" Genomics. 25. 184-191 (1995)
• [文献書誌] Naruse, T. (Inoko, H. ): "Cloned PLT cells recognize the fourth, fifth and sixth hypervariable regions at amio acid positions 65-87 of DPB1 molecule" Hum. Immunol.42. 123-130 (1995)
• [文献書誌] Altmann, DM. (Inoko, H. ): "The T cell response of HLA-DR transgenic mice to myelin basic protein and other antigens in the presence and absence of human CD4" J. Exp. Med.181. 867-875 (1995)
• [文献書誌] Horiki, T. (Inoko, H. ): "Combinations of HLA-CPB1 and HLA-DQB1 alleles determine susceptibility to early-onset myasthenia gravis in Japan" Autoimmunity. 19. 45-54 (1995)
• [文献書誌] Kuwana, M. (Inoko, H. ): "Major histocompatibility complex class II gene associations with anti-U1 small nuclear ribonucleoprotein antiboby. Relationship to immunoreactivity with individual constituent proteins" Arthritis & Rheumatism. 38. 396-405 (1995)
• [文献書誌] Ishihara, M. (Inoko, H. ): "Clinical features of sarcoidosis in relation to HLA distribution and HLA-DRB3 genotyping by PCR-RFLP" British Journal of Opthalmology. 79. 322-325 (1995)
• [文献書誌] Mizuki, N. (Inoko, H. ): "Microsattelite polymorphism between the TNF and HLA-B genes in Behcet's disease" Hum. Immunol.43. 129-135 (1995)
• [文献書誌] Ishihara, M. (Inoko, H. ): "Genetic polymorphisms of the TNFB and HSP70 genes located in the human major histocompatibility comple in sarcoidosis" Tissue Antigens. 46. 59-62 (1995)
• [文献書誌] Shiina, T. (Inoko, H. ): "Isolation and characterization of cDNA clones for Japanese quail (Coturnix japonica) major histocompatibility complex(MhcCoja) class I molecules" Immunogenetics. 42. 213-216 (1995)
• [文献書誌] Geng, L. (Inoko, H.): "Determination of HLA class II alleles by genotyping in a Manchu population in the northen part of China and its relationship with Han and Japanese population" Tissue Antigens. 46. 111-116 (1995)
• [文献書誌] Nakanishi, K. (Inoko, H. ): "Residual b-cell function and HLA-A24 in insulin-dependent diabetes mellitus: markers of glycemic control and subsequent development of diabetic retinopathy" Diabetes. 44. 1334-1339 (1995)
• [文献書誌] 成瀬 妙子(猪子 英俊): "HLAのDNAタイピング:表記法と臨床応用" 臨床免疫. 27. 242-248 (1995)
• [文献書誌] 高田 肇(猪子 英俊): "HLA-DM" 臨床免疫. 27. 627-634 (1995)
• [文献書誌] 成瀬 妙子(猪子 英俊): "DNAによるHLAタイピングの基礎(3).クラスII抗原のDNAタイピング-2-" Organ Biology. 2(1). 53-59 (1995)
• [文献書誌] 成瀬 妙子(猪子 英俊): "免疫学的検査 I. HLA HLA DNAタイピング" 日本臨床. 53. 676-683 (1995)
• [文献書誌] 安藤 麻子(猪子 英俊): "HLA領域のゲノム解析" 細胞工学. 14. 635-644 (1995)
• [文献書誌] 青山 伸郎(猪子 英俊): "潰瘍性大腸炎におけるHLAタイピング" 消化器科. 20. 311-318 (1995)
• [文献書誌] 成瀬 妙子(猪子 英俊): "DNAによるHLAタイピングの基礎(4).DNAタイピングの臨床応用-データの読み方と考え方-" OrganBiology. 2(2). 37-46 (1995)
• [文献書誌] 猪子 英俊: "ナルコレプシ-" Molecular Medicine. 98. 1344-1346 (1995)
• [文献書誌] 水木 信久(猪子 英俊): "免疫応答におけるHLAの機能" 今日の移植. 8. 529-544 (1995)
• [文献書誌] 兼重 俊彦(猪子 英俊): "最新PCR-RFLP情報:1995年版 Nomenclature for factors of the HLA systemを対象としたPCR-RFLP法によるDRB1, DPB1タイピング" MHC. 2. 76-84 (1995)
• [文献書誌] Iida, A.(Imai, T. ): "Allelic losses on chromosome 11q13 in aldosterone-producing adrenal tumors" Genes Chromo. Cancer. 12. 73-75 (1995)
• [文献書誌] Imai, T.: "Construction of YAC contigs at human chromosome 11q22.3-q23.1 region covering the ataxia telangiectasia locus" DNA Research. 2. 113-121 (1995)
• [文献書誌] Miyoshi, H. (Imai, T. ): "Alternative splicing and genomic structure of the AML1 gene involved in acute myeloid leukimia" Nicleic Acids Res.23. 2762-2769 (1995)
• [文献書誌] Gardiner, K. (Ohki, M. ): "Localization of cDNAs to a region poorly represented in the CEPH chromosome 21 YAC contig: candidate genes for genetic diseases mapped to 21sq22.3" Genomics. 30. 376-379 (1995)
• [文献書誌] Goto, M. (Ohki, M. ): "Assignment of the E4TF1-60 gene to human chromosome 21q21.2-21.3" Gene. 166. 337-338 (1995)
• [文献書誌] Ohira, M. (Ohki, M. ): "A 1.6 Mb P1-based physical map of the Down syndrome region on chromosome 21" Genomics. (in press). (1996)
• [文献書誌] Ohira, M. (Ohki, M. ): "Identification of a novel gene containing the tetratricopeptide repeat domain from the Down syndrome region of chromosome 21" DNA Res.,. (in press). (1996)
• [文献書誌] Mitelman, F. (Kaneko, Y. ). HGM, The Johns Hopkins University Press (Report of the committe in chormosome changes in neoplasia)
• [文献書誌] Kaneko, Y.: "Fusion of an ETS-family gene, ElAF, to EWS by t(17; 22)(q12; q12) chromosome translocation in an undifferential sarcoma of infancy" Genes Chromo. Cancer,. (in press).
• [文献書誌] Kato, M. (Kimura, A. ): "Altered actin binding with myosin mutation in hypertrophic cardiomyopathy and sudden death" Lancet. 345. 1247 (1995)
• [文献書誌] Nishi, H. (Kimura, A. ): "A myosin missense mutation, not a null allele, causes familial hypertrophic cardiomyopathy" Circulation. 91. 2911-2915 (1995)
• [文献書誌] Komure, O. (Kondo, I. ): "DNA analysis in hereditary dentatorubral-pallidoluysianatrophy: variation and the molecular basis of anticipation" Neurogy. 45. 143-149 (1995)
• [文献書誌] Masuda, N. (Kondo, I. ): "Analysis of triplet repeats in the huntingtin gene in Japanese families affected with Huntington's disease" J. Med. Genet.32. 701-705 (1995)
• [文献書誌] Uyama, E. (Kondo, I. ): "Dentatorubral-pallidoluysian atrophy (DRPLA): clinical, genetic, and neuroradiologic studies in a family" J. Neurological Sci.130. 146-153 (1995)
• [文献書誌] Kondo, I.: "Commentary to the paper entitled “Non-radioactive DNA diagnosis for the fragile X syndrome in Japanese mentally retarded males"" Brain & Development. 17. 323 (1995)
• [文献書誌] Morimoto, Y. (Kondo, I. ): "Association analysis of a polymorphism of the monoamine oxidase B gene with Parkinson's disease in a Japanese population" Amer. J. Med. Genet.60. 570-572 (1995)
• [文献書誌] Maguchi, M. (Kondo, I. ): "Molecular cloning and gene mapping of human basic and acidic calponins" Biochem. Biophy. Res. Comm.217(1). 238-244 (1995)
• [文献書誌] Kuwano, A. (Kondo, I. ): "Assignment of the squamous cell carcinoma antigen locus (SCC) to 18q21 by in situ hybridization" Genomics. 30. 626 (1995)
• [文献書誌] Zhao, N. (Sakaki, Y. ): "High-destiny cDNA filter analysis: a novel approach for large-scale' quantitative analysis of gene expression" Gene. 156. 207-213 (1995)
• [文献書誌] Adachi, N. (Sakaki, Y. ): "Differential display analysis of gene expression in developing embryos of Xenopus laevis" Biochimica et Biophysica Acta. 1262. 43-51 (1995)
• [文献書誌] Araki, E(Sakaki, Y. ): "Transgenic mice expressing the amyloid protein-containing carboxyl-terminal fragment of the Alzheimer amyloid precursor protein" Amyloid. 2. 100-106 (1995)
• [文献書誌] Alemeida, M. R. (Sakaki, Y. ): "Haplotype analysis of common transthyretin mutations" Hum. Genet.96. 350-354 (1995)
• [文献書誌] Kurose, K. (Sakaki, Y. ): "RNA polymerase III dependence of the human L1 promoter and possible participation of the RNA polymerase II factor YY1 in the RNA polymerase III transcription system" Nucleic Acids Res.23. 3704-3709 (1995)
• [文献書誌] Kato, M. (Sakaki, Y. ): "Altered actin binding with myosin mutation in hypertrophic cardiomyopathy and sudden death" Lancet. 345(8959). 1247 (1995)
• [文献書誌] Takahashi, N. (Sakaki, Y. ): "High-density cDNA filter analysis of the expresion profiles of the genes preferentially expressed in human brain" Gene. 164. 219-227 (1995)
• [文献書誌] Toyoda, A. (Sakaki, Y. ): "Mode of activation of the GC box/Spl-dependent promoter of the human NADH-cytochrome b5 reductase-encoding gene" Gene. 164. 351-355 (1995)
• [文献書誌] Cher, M. L. (Sakaki, Y. ): "Mapping of Regions of Physical Deletion on Chromosome 16q in Prostate Cancer Cells by Fluorescence in situ Hybridization (FISH)" J. Urol. 153. 249-254 (1995)
• [文献書誌] Ito, T. (Sakaki, Y. ): "Fluorescent differential display method for high-speed scanning of cell-specific transcripts methods" Mol. Genet.8. 229-245 (1995)
• [文献書誌] Ito, T. (Sakaki, Y. ): "Fluorescent Differential Display (FDD): A Fast and Safe Way for Reliable DD Analysis in Springer Lab Manual: Fingerprinting Methods Using PCR" Springer-Verlag, in press(Eds. Micheli, M. R. and Bova, R. ). (1996)
• [文献書誌] Shibata, A. (Sakaki, Y. ): "Identification of cis-acting elements involved in an alternative splicing of the amylod precursor protein (APP)gene" Gene,. (in press). (1996)
• [文献書誌] Hattori, M. (Sakaki, Y. ): "Mutation detection by orphan peak analysis in CRC Press book: The Detection of DNA sequenece polymorphisms and mutations" Methods and Applications,. (in press). (1996)
• [文献書誌] Suzuki, T. (Sakaki, Y. ): "Regional and cellular presenilin 1 gene expression in human and rat tissues" Biochem. Biophys. Res. Commun. , (in press). (1996)
• [文献書誌] Naruse, K. (Sakaki, Y. ): "A YAC contig of the human CC chemokine genes clustered on chromosome 17q11.2" Genomics,. (in press). (1996)
• [文献書誌] Hattori, M. (Sakaki, Y. ): "Novel method for making nested deletions and its application for sequencing of a 300 kb APP gene locus" (submitted). (1996)
• [文献書誌] Tsukahara, F. (Sakaki, Y. ): "Cloning of a novel cDNA encoding tetratricopeptide repeat (TPR) motif from Down syndrome critical region 21q22.2" (submitted). (1996)
• [文献書誌] Ito, T. (Sakaki, Y. ): "Fluorescent Differential Display Reveals a Male-Specific Band in Mouse Liver That Encodes a Member of Cytochrome P450 4A Family" (submitted). (1996)
• [文献書誌] Kito, K. (Sakaki, Y. ): "Fluorescent Differential Display Analysis of Gene Expression of Differentiating Neuroblastoma" (submitted). (1996)
• [文献書誌] Sato, S. (Sakaki, Y. ): "Splicing mutation of Presenilin 1 gene for early-onset familial Alzheimer's disease" (submitted). (1996)
• [文献書誌] Minoshima, S. (Shimizu, N. ): "Mapping of the gene for Human Xanthine Dehydrogenase (Oxidase)(XDH) to the band p23 of chromosome 2" Cytogenet. Cell Ggenet.68(1-2). 52-53 (1995)
• [文献書誌] Amagai, M. (Shimizu, N. ): "Assignment of the human genes for desmocollin 3 and desmocollin 4 to chromosome 18q12" Genomics. 25(2). 330-332 (1995)
• [文献書誌] Mashima, Y. (Shimizu, N. ): "Risk of false-positive molecular genetic diagnosis of leber's hereditary optic neuropathy" Am. J. Ophthalmol.119(2). 245-246 (1995)
• [文献書誌] Kubota, R. (Shimizu, N. ): "Mapping of the human gene for Pmel 17 (D12S53E) to chromosome 12q13-q14" Genomics. 26(2). 430-431 (1995)
• [文献書誌] Sakai, K. (Shimizu, N. ): "Human ribosomal RNA gene cluster: Identification of the proximal end containing a novel tandem repeat sequence" Genomics. 26(3). 521-526 (1995)
• [文献書誌] Chen, H. (Shimizu, N. ): "Single-minded and Down Syndrome?" Nauture Genet.10(1). 9-10 (1995)
• [文献書誌] Shimizu, N.: "Report of the Fifth International Workshop on Human Chromosome 21 Mapping 1994" Cytogenet. Cell Genet.70(3-4). 147-182 (1995)
• [文献書誌] Kudoh, J. (Shimizu, N. ): "Localization of the human AHNAK/ Desmoyokin gene (AHNAK) to chromosome band 11q12 by somatic cell hybrid analysis and fluorescence in situ hybridization" Cytogenet. Cell Genet.70(3-4). 218-220 (1995)
• [文献書誌] Wang, Y. (Shimizu, N. ): "Cot-1 banding of human chromosomes using fluorescence in situ hybridization with Cy3 labeling" Jpn. J. Human Genet.40(3)). 243-252 (1995)
• [文献書誌] Kawasaki, K. (Shimizu, N. ): "The organization of the human immunoglobulin 1 gene locus" Genome Res.5(2). 125-135 (1995)
• [文献書誌] Mashima, Y. (Shimizu, N. ): "Quantitative determination of heteroplasmy in leber's hereditary optic" Visual Sci.36(8). 1714-1720 (1995)
• [文献書誌] Tobe, T. (Shimizu, N. ): "Mapping of human inter-alpha-trypsin inhibitor family heavy chain-related protein gene (ITIHL1) to human chromosome 3p21-p14" Cytogenet. Cell Genet.71(3). 296-298 (1995)
• [文献書誌] Minoshima, S. (Shimizu, N. ): "Gene View PLUS locus-in and physical mapper: The GUI-softwares for gGenome mapping data management" Proceedings Genome Informatics Workshop 1995. 146-147 (1995)
• [文献書誌] Hattori, N. (Shimizu, N. ): "Structural organization and chromosomal localization of the human nuclear gene (NDUFV2) for 24-kDa iron-sulfur subunit of complex I in mitochondrial respiratory chain" Biochem. Biophys. Res. Commun.216(3). 771-777 (1995)
• [文献書誌] Yamaki, A. (Shimizu, N. ): "The mammalian single-minded (SIM) gene; Mouse cDNA structure and diencephalic expression imply a candidate gene for Down Syndrome" Genomics. (in press).
• [文献書誌] Osoegawa, K. (Shimizu, N. ): "An integrated map with cosmid/PAC contigs of a 4-Mb Down syndrome critical region" Genomics. (in press).
• [文献書誌] Ikeuchi, T. (Tsuji, S. ): "Dentatorubral-Pallidoluysian Atrophy (DRPLA): Clinical features are closely related to unstable expansions of trinucleotide (CAG) repeat" Ann. Neurol.37. 769-775 (1995)
• [文献書誌] Onodera, O. (Tsuji, S. ): "Molecular cloning of cDNA for dentatorubral-pallidoluysian atrophy (DRPLA) and regional expressions of the expanded alleles in the central nervous system" Am. J. Hum. Genet.57. 1050-1060 (1995)
• [文献書誌] Ichinose, H. (Tsuji, S. ): "Hereditary progressive dystonia with marked diurnal fluctuation caused by mutations in the GTP cyclohydrolase I gene" Nature Genet.8. 236-242 (1995)
• [文献書誌] Burke, J. R. (Tsuji, S. ): "Dentatorubral-Pallidoluysian atrophy and Haw River syndrome" Lancet. 344. 1711-1712 (1995)
• [文献書誌] Tanaka, H. (Tsuji, S. ): "The gene for hereditary progressive dystonia with marked diurnal fluctuation maps to chromosome 14q" Ann. Neurol.37(3). 769-775 (1995)
• [文献書誌] Ikeuchi, T. (Tsuji, S. ): "Dentatorubral-pallidoluysian atrophy (DRPLA): close correlationof GAG repeat expansions with the wide spectrum of clinical presentations and prominent anticipaiton" Seminars in Cell Biology. 6(1). 37-44 (1995)
• [文献書誌] Ikeuchi, T. (Tsuji, S. ): "Dentatorbral-pallidoluysian atrophy (DRPLA). Molecular basis for wide clinical features of DRPLA" Clin. Neurosci.3(1). 23-27 (1995)
• [文献書誌] Sanpei, K. (Tsuji, S. ): "Direct detection of expanded (CAG/CTG) repeats in the myotonin-protein kinase genes of myotonic dystrophy patients using a high-stringency hybridization method" Biochem. Biophys. Res. Commun.212(2). 341-346 (1995)
• [文献書誌] Okuizumi, K. (Tsuji, S. ): "Genetic association of the very low density lipoprotein (VLDL) receptor gene with sporadic Alzheimer's disease" Nature Genet.11. 207-209 (1995)
• [文献書誌] Onodera, O. (Tsuji, S. ): "Molecular cloning of a full-length cDNA for dentatorubral-pallidoluysian atrophy and regional expression of the expanded alleles in the CNS" Am. J. Hum. Genet.57(5). 1050-1060 (1995)
• [文献書誌] Takiyama, Y. (Tsuji, S. ): "Evidence for inter-generational instability in the CAG repeat in the MJDI gene and for conserved haplotypes at flanking markers amongst Japanese and Caucasian subjects with Machado-Joseph disease" Hum. Mol. Genet.4(7). 1137-1146 (1995)
• [文献書誌] Koike, R. (Tsuji, S. ): "Partial deletions of putative adrenoleukodystrophy (ALD) gene in Japanese ALD patietns" Hum. Mutation. 6. 263-267 (1995)
• [文献書誌] Yoneda, M. (Tsuji, S. ): "Detection and quantification of point mutations in mtDNA by PCR" Methods in Enzymology,. (in press).
• [文献書誌] Toda, T. (Nakahori, Y. ): "Genetic identity of Fukuyama type congenital muscular dystrophy and Walker-Warburg syndrome" Ann. Neurol.37. 99-101 (1995)
• [文献書誌] Shiojima, I. (Nakahori, Y. ): "Assignment of Cardiac-specific homeobox gene CSX to human chromosome 5q34" Genomics. 27. 204-206 (1995)
• [文献書誌] Muroya, K. (Nakahori, Y. ): "Refinement of the locus for X-linked recessive chondrodysplasia punctata by genotype-phenotype correlations" Hum. Genet.95. 577-580 (1995)
• [文献書誌] Ogata, T. (Nakahori, Y. ): "Chromosomal localization of a Y-specific growth gene(s)" J. Med. Genet. 32. 572-575 (1995)
• [文献書誌] Okada, T. (Nakahori, Y. ): "46, XY female karyotype containing SRY" Clin. Pediatr. Endocrinol.4. 103-107 (1995)
• [文献書誌] Iwamoto, T. (Nakahori, Y. ): "Pericentric inversion with a minute deletion of the Y chromosome" Clin. Genet.48. 272-274 (1995)
• [文献書誌] Nakahori, Y.: "The incidence of the fragile X syndrome in Japanese (Commentary)" Brain & Development. 17. 322 (1995)
• [文献書誌] Toda, T. (Nakahori, Y. ): "Dinucleotide repeat polymorphism on chromosome 9q32" Jpn. J. Hum. Genet. 40. 333-334 (1995)
• [文献書誌] Naruse, T. (Inoko, H. ): "Advances in HLA typing, Proceedings of the 11th Japan HLA Workshop (eds. Sonoda S, Yashiki S, Fujinobu T) The PCR-RFLP method for HLA class II genotyping" MHC & IRS, 65-72 (1995)
• [文献書誌] Oishi, H. (Inoko, H. ): "Advances in HLA typing, Proceedings of the 11th Japan HLA Workshop (eds. Sonoda S, Yashiki S, Fujinobu T) Smitest HLA DNA typing system: HLA class II typing with PCR-RFLPmethod" MHC & IRS, 73-95 (1995)
• [文献書誌] 猪子 英俊: "バイテクノロジー概論、モノクローナル抗体" 朝倉書店, 44-62 (1995)
• [文献書誌] 猪子 英俊: "タンパク質化学9 脳・神経タンパク質、接着タンパク質(野村靖幸編) MHC(Major Histocompatibility Complex)" 広川書店, 337-344 (1995)
• [文献書誌] 猪子 英俊: "Bio Science用語ライブラリー 免疫(斎藤隆、竹森利忠編)、MHC遺伝子" 羊土社, 62-64 (1995)
• [文献書誌] 今井 高志: "Vectoretteを利用した隣接未知配列の増幅 実験医学別冊 バイオマニュアルUPシリーズ(林健志編)PCR法の最新技術" 羊土社, 84-92 (1995)
• [文献書誌] 今井 高志: "癌関連遺伝子プローブ、遺伝子断片の調製法:コスミド、YAC 実験医学別冊 バイオマニュアルシリーズ(横田淳、山本雅編) 分子生物学的アプローチによる癌研究プロトコール" 羊土社, 26-34 (1995)
• [文献書誌] 金子 安比古: "転座関連遺伝子AMLl. Annual Review血液1995. (高久史麿、宮崎澄雄、斎藤英彦、溝口秀昭編)" 中外医学社, 110-117 (1995)
• [文献書誌] Kato, M. (Kimura, A. ): "Genetic approaches to identification of a novel responsible gene for hypertrophic cardiomyopathy. (ed. Yazaki, Y. ) Cardiac development and gene regulation" Excerpta Medica, 127-144 (1995)
• [文献書誌] Antonarakis, S. E. (Shimizu, N. ): "Report of the committee on the genetic constitution of chromosome 21 Human Gene Mapping 94(ed. Cuticchia, AJ)" The Johns Hopkins Univ. Press, Baltimore, 732-766 (1995)
• [文献書誌] Antonarakis, S. E. (Shimizu, N. ): "Report of the committee on the genetic constitution of chromosome 21 Human Gene Mapping 95(ed. Cuticchia, AJ)" The Johns Hopkins Univ. Press, Baltimore, in press,
• [文献書誌] 中堀 豊: "遺伝性疾患の遺伝子診断:眼科学体系10A、目の発生と遺伝" 中山書店, 97-106 (1995)
• [文献書誌] 中堀 豊: "脆弱X症候群:臨床DNA診断法" 金原出版, 628-629 (1995)
• [文献書誌] 林 健志(編): "PCR法の最新技術 実験医学別冊" 羊土社, (1995)
• [文献書誌] 藤山 秋佐夫: "染色体操作技術 組織培養" 5-6 (1996)
• [文献書誌] 藤山 秋佐夫: "ヒト染色体のソーティング 組織培養" 47-51 (1996)
• [文献書誌] Honjo, T. and Matsuda, F.: "Immunoglobulin heavy chain loci of mouse and man Immunoglobulin Genes(T. Honjo and FW. Alt, eds.)" Academic Press, London, U. K., 145-171 (1995)
• [文献書誌] Matsuda, F. and Honjo, T.: "Organization of the human immunoglobulin heavy-chain locus Advances in Immunology(F. Dixon, ed.)" Academic Press, San Diego, USA. in press,
• [文献書誌] Yoshikawa, A. (Miki, T. ): "Association study of apolipoprotein E and sporadic Alzheimer's disease in the Japanese population(eds. Iqbal, K. , Maritmer, JA. , Winbald, B. ) Research Advances in Alzheimer's Disease and Related Disorders" John Wiley & Sons Ltd., 113-120 (1995)
• [文献書誌] 石川 冬木: "ゲノム・クライシス-何が腫瘍をひき起こすか 化学と生物 33" 635-644 (1995)
• [文献書誌] 石川 冬木: "細胞の寿命はどのようにして決まるのか 現代化学10月号" 44-49 (1995)
• [文献書誌] 石川 冬木: "テロメレースの生化学 実験医学13" 羊土社, 1491-1496 (1995)
• [文献書誌] 石川 冬木: "染色体テロメアとPosition Effect 実験医学 13" 羊土社, 1378-1383 (1995)
• [文献書誌] 石川 冬木: "テロメアと真核生物染色体の維持機構 細胞工学 14" 秀潤社, 885-893 (1995)
• [文献書誌] 石川 冬木: "癌の病態と遺伝子診断 Quality Nursing 1" 101-106 (1995)
• [文献書誌] 鮎澤 大: "細胞老化と不死化 Molecular Medicine 33" 176-183 (1996)
• [文献書誌] 小原 雄治: "線虫C.エレガンスの細胞死の遺伝子カスケード 「アポトーシスの分子医学」(橋本嘉幸、山田武 編)" 羊土社, 26-36 (1995)
• [文献書誌] 小原 雄治: "線虫C. elegansゲノムの発現マップ、細胞工学 14" 秀潤社, 661-666 (1995)
• [文献書誌] 小原 雄治: "座談会、情報生物学の現状と将来、蛋白質核酸酵素 40" 共立出版, 1784-1802 (1995)
• [文献書誌] 小原 雄治: "線虫cDNAの大量解析 蛋白質核酸酵素" 共立出版、(印刷中), 1784-1802 (1995)
• [文献書誌] 西口 聖治 (島田 和典): "アミロイド-シイス図説分子病態学(一瀬白帝、鈴木宏治編)" 中外医学社, 353-358 (1995)
• [文献書誌] 島田 和典: "遺伝子診断と遺伝子治療、病気のサイエンス、大阪大学微生物病研究所編" 大阪大学出版会, 178-187 (1995)
• [文献書誌] 藤原 力 (高橋 永一): "脳cDNAの大量解析と機能解析へのアプローチ 特集ゲノムバイオロジー 細胞工学 14" 秀潤社, 645-654 (1995)
• [文献書誌] 深川 竜郎 (池村 淑道): "GC%の巨大区分構造が語るもの -ゲノム進化の視点から 実験医学 13" 羊土社, 1365-1370 (1995)
• [文献書誌] 奥村 克純: "FISHによるDNA複製タイミングの解析とゲノム・インプリンティング 組織培養 21(4)" 131-134 (1995)
• [文献書誌] 馬場 嘉信: "キャピラリー電気泳動 基礎と実験 (本田、寺部編)" 講談社, (1995)
• [文献書誌] 馬場 嘉信: "機器分析におけるコンピュータ利用 (日本分析化学会編)" 朝倉書店, (1995)
• [文献書誌] 馬場 嘉信: "基礎化学コース 分析化学III (井上、北森、小宮山、高木、平野編)" 丸善(印刷中), (1995)
• [文献書誌] 馬場 嘉信: "薬学領域における機器分析 (中村編)" 朝倉書店(印刷中), (1995)
• [文献書誌] 馬場 嘉信: "最新の分離・精製・検出法 (梅澤、澤田、中村編)" エヌ・ティー・エス出版(印刷中), (1995)
• [文献書誌] 馬場 嘉信: "医療におけるバイオテクノロジー (木村編)" 医薬ジャーナル(印刷中), (1995)
• [文献書誌] Baba, Y.: "Capillary Gel Electrophoresis and Related Techniques" Elsevier, Amsterdam, in press, (1995)
• [文献書誌] 林崎 良英: "ゲノム解析研究と医学分野における将来への展望" Pharma Medica, 51-56 (1995)
• [文献書誌] 林崎 良英: "DNA多型検出の戦略 DNA多型 3" 東洋書店, 10-15 (1995)
• [文献書誌] Hayashizaki, Y.: "Restriction landmark genomic scanning (RLGS), Molecular Biology and Biotechnology" VCH publishers, 813-817 (1995)
• [文献書誌] 松田 洋一: "染色体のin situハイブリダイゼーション 組織培養の技術 3" 朝倉書店(印刷中), (1995)