1997 年度実績報告書

細胞増殖因子、形態形成因子の細胞分化における役割

研究課題

研究課題/領域番号	08045066
研究機関	徳島大学
研究代表者	荒瀬誠治徳島大学, 医学部, 教授 (90108887)
研究分担者	FRANCE J.T. オークランド大学, 医学部, 準教授 CROSIER K.E. オークランド大学, 医学部, 準教授 MERRILEES M. オークランド大学, 医学部, 副学長小阪昌明徳島大学, 医学部, 講師 (10093858) 板倉光夫徳島大学, 医学部, 教授 (60134227)
キーワード	糖尿病 / TGF-β / トランスゼニックマウス / amylin / activin / 同種抹消血移植 / 毛乳頭特異的遺伝子 / 動脈粥状変成 / gap junction
研究概要	板倉は、インスリン依存性糖尿病(IDDM)マウスの膵ラ島A細胞に活性型TGF-β1を発現するトランスジェニックマウス(Tg)を作成し、NODマウスの糖尿病の発症が抑制されることを見出した。CooperはAmylinと糖尿病の関係をTgやノックアウトマウスで検討した。小坂は骨髄造血環境での単球、骨髄間質線維芽細胞のActivin-A(Act-A)、follistatin(FS)の産生が、炎症性サイトカインや活性化T細胞の接着を介する細胞間相互作用により調節されることを見出し、Franceは胎盤由来Act-A、CRHの量比が分娩の引き金となることを証明、血清中の両者の量のモニターで、早期分娩の危険性を察知できるとした。河野は小児の自家末梢血幹細胞移植術法(PBSCT)では、PBSCT後のG-CSF投与は必要ないことを見出した。また小児健常人ドナーから末梢血幹細胞(PBSC)を採取する同種移植術も確立した。Crosierらは新型受容体チロシンキナーゼ(Dtk)遺伝子のcDNA、遺伝子座、ligand、機能を検討し、Dtkは胎児期の造血臓器、神経の発生・発育にも必須であることを見出した荒瀬は28個のヒト毛乳頭特異的新型遺伝子を見出し、そのうち10個の新規遺伝子のDNA配列を決定、遺伝子産物機能や皮膚での局在を解析中でノックアウトマウスも作成中である。Greenは、ヒドラ、蛙などの胎児組織にgapjunctionのantisense deoxyoligonucleotide作用させると臓器の形成が妨げられることより、臓器の形成・発育にはGap Junctionを通じての細胞間情報交換が必須であることを見出した。Merrileesは、動脈壁でのTGF-β1とmatrix proteoglycan(Pgとくにversican、biglycan、decorin)の量と分布が粥状変成の危険度が類推できることを見出した。

研究成果

(42件)

すべてその他

すべて文献書誌 (42件)

[文献書誌] Takiwaki H, Arase S et al: "Computor simulation of skin color based on a multilayered skin model" Skin Res Technol. 3. 36-41 (1997)
[文献書誌] Takiwaki H, Arase S et al: "A simple method to estimate CIE-L l.a.b.values of the skin from its videomicroscopic image" Skin Res Technol. 3. 42-44 (1997)
[文献書誌] Ninomiya Y, Arase S et al: "P53 gene mutation analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J Dermatol. 14. 172-178 (1997)
[文献書誌] Kubo Y, Arase S et al: "Mutations of the INK4a locus in squamous cell carcinomas of human skin" BBRC. 232. 38-41 (1997)
[文献書誌] Shikiji T, Arase S et al: "A case of episodic angioedema associated with eosinophilia" J Med Invest. 44. 103-108 (1997)
[文献書誌] Miyamoto H, Arase S et al: "Color analysis of nevus of Ota for evaluation of treatment with a Q-switched alexandrite laser." Skin Res Technol. 3. 45-48 (1997)
[文献書誌] Miyaoka Y, Arase S et al: "A case of dermatomyositis complicated by thrombotic thrombocytepenic purpura" Dermatology. 194. 68-71 (1997)
[文献書誌] Hamaoka H, Arase S et al: "Effect of K^+ channel openers on K^+ channel in cultured human dermal papilla cells." J Med Invest. 44. 73-77 (1997)
[文献書誌] kunika K, Yamaoka Y, Itakura M: "Damage of Charge Dependent Renal Tubular Reabsorption Causes Diabetic Microproteinuria" Diabet.Res.Clin.Pract. 36. 1-9 (1997)
[文献書誌] Sarui H, Itakura M et al: "Cystic Glucagonoma with Lossof Heterozygosity on Chromosome 11 in Multiple Endocrine Neoplasia Type 1." Clin.Endocrinol. 46. 511-516 (1997)
[文献書誌] Tomonari A, Itakura M et al: "Proximal Nucleotide Sequence of the Humang-GlutamylcysteineSynthetase Heavy Subunit Gene is Involved in Cisplatin-Induced Transcriptional Up-Regulation in a Lung Cancer Cell Line SBC3." Biochem.Biophys.Res.Commun. 236. 616-621 (1997)
[文献書誌] Ninomiya Y, Itakura M et al: "p53 Gene Mutation Analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J.Dermatol.Science. 14. 173-178 (1997)
[文献書誌] MinH et al: "p53 Gene Mutaion in N-Butyl-N-(4-hydroxybutyl)nitrosamine-Induced Urinary Bladder Tumors and N-Methyl-N-nitrosourea-induced Colon Tumors of LEC and F344 Rats" 117. 81-86 (1997)
[文献書誌] Yamaoka T, Itakura M et al: "Amidophosphoribosyltransferase Limits the Rate of Cell Growth-linked De Novo Purine Biosynthesis in the Presence of Constant Capacity of SalvagePurine Biosynthesis." J.Biol.Chem. 272. 17719-17725 (1997)
[文献書誌] Tanaka C, Itakura M et al: "Infrequent Mutations of p27Kip1 Gene and Trisomy 12 in a Subset of Human Pituitary Adenomas" J.Clin.Endocrinol.Matab. 82. 3141-3147 (1997)
[文献書誌] Ozaki S, Kosaka M, et al: "Thrombopoietin-responsive essential thrombocythemia with myelofibrosis" Brit I Haematol. 97. 449-452 (1997)
[文献書誌] Ozaki S, Kosaka M et al: "Immunotherapy of multiple myeloma with a monoclonal antibody directed against a plasma cell-specific antigenn,HM1.24" Blood. 90. 3179-3186 (1997)
[文献書誌] Shiozaki M, Kosaka M, Eto Y: "Activin A ; a commiment factor in erythroid differentiation" BBRC. 242. 631-635 (1998)
[文献書誌] 小坂昌明、尾崎修治: "ヒト形質細胞腫モデルと抗ヒト骨髄主細胞抗体の抗腫瘍効果" 臨床血液. 38. 275-280 (1997)
[文献書誌] 阿部正博、小坂昌明他: "多発性骨髄腫" 日本医事新報. 3833. 33-36 (1997)
[文献書誌] Sakuma H, Kawano Y et al.: "Risk of cytomegalovirus infection after peripheral blood stem cell transplantation" Bone-Marrow-Transplant. 19. 49-53 (1997)
[文献書誌] Abe T, Kawano Y et al: "Transduction of retrovirus-mediated NeoR gene into CD34_+ cells purified from granulocyte colony-stimulating factor (G-CSF)-mobilized infant and cord blood" Exp-Hematol. 25. 966-971 (1997)
[文献書誌] Okamoto Y, Kawano Y et al: "Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34_+ cells." Cancer-Res. 57. 5037-5040 (1997)
[文献書誌] Abe T, Kawano Y et al: "Effect of recombinant erythropoietin in interaction with stromal factors on cord blood hematopoiesis" Blood.87. 3212-3217 (1996)
[文献書誌] Scott L, Merrilees MJ et al: "Subendothelial proteoglycan synthesis and transforming growth factor beta distribution correlate with susceptibility to atherosclerosis" J-Vasc-Res. 34. 365-377 (1997)
[文献書誌] Poole CA, Merrilees MJ et al: "Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1" Connect-Tissue-Res. 33. 333-341 (1996)
[文献書誌] Merrilees MJ, Beaumont BW, Scott LJ: "Fluoroprobe quantification of viabie and non-viable cells in human coronary and intemal thoracic arteries sampled at autopsy" J-Vasc-Res. 32. 371-377 (1995)
[文献書誌] Davidson AJ, Crosier KE et al: "Expression of murine interleukin 11 and its receptor alpha-chain in adult and embryonic tissues." Stem-Cells. 15. 119-124 (1997)
[文献書誌] Crosier KEet al: "Expression and functional analysis of two isoforms of the human GM-CSF receptor alpha chain in myeloid development and leukaemia" Br-J-Haematol.98. 540-548 (1997)
[文献書誌] Crosier KE, Crosier PS: "New insights into the control of cell growth ; the role of the Axl family." Pathology. 29. 131-135 (1997)
[文献書誌] Lewis PM, Crosier KE et al: "Analysis of the murine Dtk gene identifies conservation of genomic structure within a new receptor tyrosine kinase subfamily" Genomics. 31. 13-19 (1996)
[文献書誌] Crosier PS, Crosier KE et al: "The Dtk receptor tyrosine kinase,which binds protein S,is expressed during hematopoiesis" Exp-Hematol. 24. 318-323 (1996)
[文献書誌] Holdaway IM, France JT et al: "Characteristics of the menstrual cycle at the time of surgery for breast cancer" Br-J-Cancer. 75. 413-416 (1997)
[文献書誌] Song Y, Keelan J, France JT: "Activin-A stimulates,while transforming growth factor beta 1 inhibits,chorionic gonadotrophin production and aromatase activity in cultured human placental trophoblasts." Placenta.17. 603-610 (1996)
[文献書誌] France JT et al: "Serum concentrations of human chorionic gonadotrophin and immunoreactive inhibin in early pregnancy and recurrent miscarriage : a longitudinal study." Aust-N-Z-J-Obstet-Gynaecol. 36. 325-330 (1996)
[文献書誌] Donaldxon P, Green CR et al: "Gap junctional channels : new roles in disease" Histology and Histopathology. 12. 219-231 (1997)
[文献書誌] Blackbum JP, Green CR et al: "Connexin 43 gap Junction levels during development of the thoracic aorta are temporally correlated with elastic laminae deposition andincreases blod pressure" Cell Biol Int. 21. 87-97 (1997)
[文献書誌] Walker MM, Green CR et al: "Structure and function of the vertebrate magnetic sense" Nature. 390. 371-376 (1997)
[文献書誌] Bond J, Green CR et al: "Liquefaction of cortical tissue in diabetic and galactosemic rat lenses defined by confocal laser scanning microscopy" Invest Opthal Visual Sci. 37. 1557-1565 (1996)
[文献書誌] Charles CJ, Cooper GJ et al: "Hemodynamic,hormonal,and renal effects of adrenomedullin in conscious sheep." Am-J-Physiol. 272. 2040-2047 (1997)
[文献書誌] Goldsbury CS, Cooper GJ et al: "Polymorphic fibrillar assembly of human amylin" J Struct Biol.119. 17-27 (1997)
[文献書誌] Cooper GJ, Tse-CA: "Amylin,amyloid and age-related disease" Drugs-Aging.9. 202-212 (1996)

1997 年度 実績報告書

細胞増殖因子、形態形成因子の細胞分化における役割

研究代表者

荒瀬 誠治 徳島大学, 医学部, 教授 (90108887)

研究成果

[文献書誌] Takiwaki H, Arase S et al: "Computor simulation of skin color based on a multilayered skin model" Skin Res Technol. 3. 36-41 (1997)

[文献書誌] Takiwaki H, Arase S et al: "A simple method to estimate CIE-L l.a.b.values of the skin from its videomicroscopic image" Skin Res Technol. 3. 42-44 (1997)

[文献書誌] Ninomiya Y, Arase S et al: "P53 gene mutation analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J Dermatol. 14. 172-178 (1997)

[文献書誌] Kubo Y, Arase S et al: "Mutations of the INK4a locus in squamous cell carcinomas of human skin" BBRC. 232. 38-41 (1997)

[文献書誌] Shikiji T, Arase S et al: "A case of episodic angioedema associated with eosinophilia" J Med Invest. 44. 103-108 (1997)

[文献書誌] Miyamoto H, Arase S et al: "Color analysis of nevus of Ota for evaluation of treatment with a Q-switched alexandrite laser." Skin Res Technol. 3. 45-48 (1997)

[文献書誌] Miyaoka Y, Arase S et al: "A case of dermatomyositis complicated by thrombotic thrombocytepenic purpura" Dermatology. 194. 68-71 (1997)

[文献書誌] Hamaoka H, Arase S et al: "Effect of K^+ channel openers on K^+ channel in cultured human dermal papilla cells." J Med Invest. 44. 73-77 (1997)

[文献書誌] kunika K, Yamaoka Y, Itakura M: "Damage of Charge Dependent Renal Tubular Reabsorption Causes Diabetic Microproteinuria" Diabet.Res.Clin.Pract. 36. 1-9 (1997)

[文献書誌] Sarui H, Itakura M et al: "Cystic Glucagonoma with Lossof Heterozygosity on Chromosome 11 in Multiple Endocrine Neoplasia Type 1." Clin.Endocrinol. 46. 511-516 (1997)

[文献書誌] Tomonari A, Itakura M et al: "Proximal Nucleotide Sequence of the Humang-GlutamylcysteineSynthetase Heavy Subunit Gene is Involved in Cisplatin-Induced Transcriptional Up-Regulation in a Lung Cancer Cell Line SBC3." Biochem.Biophys.Res.Commun. 236. 616-621 (1997)

[文献書誌] Ninomiya Y, Itakura M et al: "p53 Gene Mutation Analysis in porokeratosis and porokeratosis-associated squamous cell carcinoma" J.Dermatol.Science. 14. 173-178 (1997)

[文献書誌] MinH et al: "p53 Gene Mutaion in N-Butyl-N-(4-hydroxybutyl)nitrosamine-Induced Urinary Bladder Tumors and N-Methyl-N-nitrosourea-induced Colon Tumors of LEC and F344 Rats" 117. 81-86 (1997)

[文献書誌] Yamaoka T, Itakura M et al: "Amidophosphoribosyltransferase Limits the Rate of Cell Growth-linked De Novo Purine Biosynthesis in the Presence of Constant Capacity of SalvagePurine Biosynthesis." J.Biol.Chem. 272. 17719-17725 (1997)

[文献書誌] Tanaka C, Itakura M et al: "Infrequent Mutations of p27Kip1 Gene and Trisomy 12 in a Subset of Human Pituitary Adenomas" J.Clin.Endocrinol.Matab. 82. 3141-3147 (1997)

[文献書誌] Ozaki S, Kosaka M, et al: "Thrombopoietin-responsive essential thrombocythemia with myelofibrosis" Brit I Haematol. 97. 449-452 (1997)

[文献書誌] Ozaki S, Kosaka M et al: "Immunotherapy of multiple myeloma with a monoclonal antibody directed against a plasma cell-specific antigenn,HM1.24" Blood. 90. 3179-3186 (1997)

[文献書誌] Shiozaki M, Kosaka M, Eto Y: "Activin A ; a commiment factor in erythroid differentiation" BBRC. 242. 631-635 (1998)

[文献書誌] 小坂 昌明、尾崎 修治: "ヒト形質細胞腫モデルと抗ヒト骨髄主細胞抗体の抗腫瘍効果" 臨床血液. 38. 275-280 (1997)

[文献書誌] 阿部 正博、小坂 昌明 他: "多発性骨髄腫" 日本医事新報. 3833. 33-36 (1997)

[文献書誌] Sakuma H, Kawano Y et al.: "Risk of cytomegalovirus infection after peripheral blood stem cell transplantation" Bone-Marrow-Transplant. 19. 49-53 (1997)

[文献書誌] Abe T, Kawano Y et al: "Transduction of retrovirus-mediated NeoR gene into CD34_+ cells purified from granulocyte colony-stimulating factor (G-CSF)-mobilized infant and cord blood" Exp-Hematol. 25. 966-971 (1997)

[文献書誌] Okamoto Y, Kawano Y et al: "Serum thrombopoietin levels in patients receiving high-dose chemotherapy with support of purified peripheral blood CD34_+ cells." Cancer-Res. 57. 5037-5040 (1997)

[文献書誌] Abe T, Kawano Y et al: "Effect of recombinant erythropoietin in interaction with stromal factors on cord blood hematopoiesis" Blood.87. 3212-3217 (1996)

[文献書誌] Scott L, Merrilees MJ et al: "Subendothelial proteoglycan synthesis and transforming growth factor beta distribution correlate with susceptibility to atherosclerosis" J-Vasc-Res. 34. 365-377 (1997)

[文献書誌] Poole CA, Merrilees MJ et al: "Detection of viable and non-viable cells in connective tissue explants using the fixable fluoroprobes 5-chloromethylfluorescein diacetate and ethidium homodimer-1" Connect-Tissue-Res. 33. 333-341 (1996)

[文献書誌] Merrilees MJ, Beaumont BW, Scott LJ: "Fluoroprobe quantification of viabie and non-viable cells in human coronary and intemal thoracic arteries sampled at autopsy" J-Vasc-Res. 32. 371-377 (1995)

[文献書誌] Davidson AJ, Crosier KE et al: "Expression of murine interleukin 11 and its receptor alpha-chain in adult and embryonic tissues." Stem-Cells. 15. 119-124 (1997)

[文献書誌] Crosier KEet al: "Expression and functional analysis of two isoforms of the human GM-CSF receptor alpha chain in myeloid development and leukaemia" Br-J-Haematol.98. 540-548 (1997)

[文献書誌] Crosier KE, Crosier PS: "New insights into the control of cell growth ; the role of the Axl family." Pathology. 29. 131-135 (1997)

[文献書誌] Lewis PM, Crosier KE et al: "Analysis of the murine Dtk gene identifies conservation of genomic structure within a new receptor tyrosine kinase subfamily" Genomics. 31. 13-19 (1996)

[文献書誌] Crosier PS, Crosier KE et al: "The Dtk receptor tyrosine kinase,which binds protein S,is expressed during hematopoiesis" Exp-Hematol. 24. 318-323 (1996)

[文献書誌] Holdaway IM, France JT et al: "Characteristics of the menstrual cycle at the time of surgery for breast cancer" Br-J-Cancer. 75. 413-416 (1997)

[文献書誌] Song Y, Keelan J, France JT: "Activin-A stimulates,while transforming growth factor beta 1 inhibits,chorionic gonadotrophin production and aromatase activity in cultured human placental trophoblasts." Placenta.17. 603-610 (1996)

[文献書誌] France JT et al: "Serum concentrations of human chorionic gonadotrophin and immunoreactive inhibin in early pregnancy and recurrent miscarriage : a longitudinal study." Aust-N-Z-J-Obstet-Gynaecol. 36. 325-330 (1996)

[文献書誌] Donaldxon P, Green CR et al: "Gap junctional channels : new roles in disease" Histology and Histopathology. 12. 219-231 (1997)

[文献書誌] Blackbum JP, Green CR et al: "Connexin 43 gap Junction levels during development of the thoracic aorta are temporally correlated with elastic laminae deposition andincreases blod pressure" Cell Biol Int. 21. 87-97 (1997)

[文献書誌] Walker MM, Green CR et al: "Structure and function of the vertebrate magnetic sense" Nature. 390. 371-376 (1997)

[文献書誌] Bond J, Green CR et al: "Liquefaction of cortical tissue in diabetic and galactosemic rat lenses defined by confocal laser scanning microscopy" Invest Opthal Visual Sci. 37. 1557-1565 (1996)

[文献書誌] Charles CJ, Cooper GJ et al: "Hemodynamic,hormonal,and renal effects of adrenomedullin in conscious sheep." Am-J-Physiol. 272. 2040-2047 (1997)

[文献書誌] Goldsbury CS, Cooper GJ et al: "Polymorphic fibrillar assembly of human amylin" J Struct Biol.119. 17-27 (1997)

[文献書誌] Cooper GJ, Tse-CA: "Amylin,amyloid and age-related disease" Drugs-Aging.9. 202-212 (1996)

1997 年度実績報告書

荒瀬誠治徳島大学, 医学部, 教授 (90108887)

[文献書誌] 小坂昌明、尾崎修治: "ヒト形質細胞腫モデルと抗ヒト骨髄主細胞抗体の抗腫瘍効果" 臨床血液. 38. 275-280 (1997)

[文献書誌] 阿部正博、小坂昌明他: "多発性骨髄腫" 日本医事新報. 3833. 33-36 (1997)