1998 年度実績報告書

細胞増殖因子、形態形成因子の細胞分化における役割

研究課題

研究課題/領域番号	08045066
研究機関	徳島大学
研究代表者	荒瀬誠治徳島大学, 医学部, 教授 (90108887)
研究分担者	クーパー G.J.S. オークランド大学, 医学部, 教授メリリース M.J. オークランド大学, 医学部, 準教授河野嘉文徳島大学, 医学部, 講師 (20260680) 小阪昌明徳島大学, 医学部, 助教授 (10093858) 板倉光夫徳島大学, ゲノム機能研究センター, センター長(研究職) (60134227)
キーワード	遺伝子 / 毛乳頭 / ランゲルハンス細胞 / 同種血液幹細胞移植 / ギャップジャンクション / チロシンキナーゼ / 動脈硬化 / TGF-β
研究概要	交流実績平成11年2月4日-10日にG・クーパー教授が徳島大学を訪問、板倉光夫教授を中心にアミリンと糖尿病、精神分裂病等との関係について検討した。また“アミリン、アミロイドと糖尿病"のタイトルで学生・大学院生の特別講義を行った。平成11年3月16日-20日には、荒瀬誠治、板倉光夫、小阪昌明の3人がオークランド大学を訪問し、研究の総括の最終的会議を開催する。なお来年度より大学院生だけでなく医学部学生の短期交換が決定された。研究実績荒瀬は、ヒト毛包の毛乳頭細胞で発現する特異的遺伝子を採取し、遺伝子解析、機能解析を行った。板倉は膵ランゲルハンス細胞の発育・機能に関与する遺伝子について、トランスゼニック、ノックアウト動物を作り遺伝子機能解析を行った。河野はHLA不一致のドナーにG-CSFを投与し末梢血幹細胞を採取、さらに抗CD34抗体を用いて細胞を順化培養し、同種末梢血幹細胞移植法を確立した。小阪は成人の血液悪性腫瘍例に同様方法を用いて治療し成果を得た。グリーンは創傷治癒へのギャップジャンクション(GP)の関与を検討し、脳では損傷後にゆっくりと起こってくる創傷拡大現象がGP発現を抑えることで抑止され、逆に皮膚の創傷治癒は著明に促進されることを見出した。クローサーらは成長に関与するテロシンキナーゼ(Dtk)のcDNAsの全配列を決め機能解析を行っている。メリリースは、動脈硬化とバーシカンやビグリカンの関係を、ヒト冠血管と培養細胞を用いて調べ、TGF-β1はこれらのプロテオグリカン産生を促進することを見出した。フランスは、胎盤とアクチビン、インヒビン、コルチコトロビン放出ホルモンの関係を研究、小坂昌明との共同で妊婦の血中アクチビン放出ホルモン量を調べ、早産の危険性との関係について報告している。

研究成果

(31件)

すべてその他

すべて文献書誌 (31件)

[文献書誌] Absan-MK.Urano-Y.et al: "Immunohistochemical localization of thyroid hormone nuclear receptors in human hair follicles and in vitro effect of L-triiodothyronine on cultured cells of hair follicles and skin" J-Med-Invest.44. 179-184 (1998)
[文献書誌] Matsumoto-K;Urano-Y et al: "Mutation of the fibroblast growth factor receptor 2 gene in Japanese patients with Apert syndrome." Plast-Reconstr-Surg. 101(2). 307-311 (1998)
[文献書誌] Kubo Y,Urano et al: "Lack of mutation in the INK4a locus in basal cell carcinoma." Br J Dermatol. 139. 340-341 (1998)
[文献書誌] Hamaoka-H;Minakuchi et al.: "Effect of K+ channel openers on K+ channcl in cultured human dermal papilla cells" J-Med-Invest. 44. 73-77 (1997)
[文献書誌] Moritani-M;Yoshimoto-K et al: "Abrogation of autoimmune diabetes in nonobese diabetic mice and protection against effector lymphocytes by transgenic paracrine TGF-betal" J-Clin-Invest.102. 499-506 (1998)
[文献書誌] Tanaka-C;Kimura-T et al: "Analysis of loss of heterozygosity on chromosome II and infrequent inactivation of the MEN1 gene in sporadic pituitary adcnomas[see comments]" J-Clin-Endocrinol-Metab. 83. 2631-2634 (1998)
[文献書誌] Kawano-Y;Takaue-Y.et al: "Partially mismatched pediatric transplants with allogeneic CD34(+) blood cells from a related donor" Blood. 92. 3123-3130 (1998)
[文献書誌] Abe-T;Makimoto-A et al: "Intra-apheresis recruitment of blood progenitor cells in children" Transfusion. 38. 944-950 (1998)
[文献書誌] Kawano-Y;Takaue-Y et al.: "Peripheral blood stem cell mobilization with granulocyte colony-stimulating factor and a harvesting procedure in pediatric donors" Bone-Marrow-Transplant. 21.S3. S32-34 (1998)
[文献書誌] Yamanaka-H;Takeda-E et al: "Total parenteral nutrition on energy metabolism in children undergoing autologous peripheral blood stem cell transplantation." J-Med-Invest.44. 199-203 (1998)
[文献書誌] Kajiume-T;Kawano-Y et al: "New consecutive high-dose chemotherapy modality with fractionated blood stem cell support in the treatment of high-risk pediatric solid tumors:a feasibility study" Bone-Marrow-Transplant. 21. 147-151 (1998)
[文献書誌] Merrilees,MJ,Beaumont B et al: "Inhibition of proteoglycan synthesis in balloon-injured rat and rabbit carotid arteries exposed to TGF-b1 antisense S-oligonucleotide." J.Vas.Res(In press). (in press). (1999)
[文献書誌] Hamaoka-T;Idehara-C et al: ":Hypoplasia of pancreatic islets in transgenic mice expressing activin receptor mutants" J-Clin-Invest. 102. 294-301 (1998)
[文献書誌] Tanaka-C;Yoshimoto-K,et al: "Absence of germ-line mutations of the multiple endocrine neoplasia type I(MEM1)gene in familial pituitary adenoma in contrast to MEN1 in Japanese" J-Clin-Endocrinol-Metab. 83. 960-965 (1998)
[文献書誌] Matsushita-T;Yamaoka-T,et al: "Molecular cloning of mouse paired-box-containing gene(Pax)-4 from an islet beta cell lineand deduced sequence of human Pax-4." Biochem-Biophys-Res-Commun. 242. 176-180 (1998)
[文献書誌] Shiozaki-M;Kosaka-M;Eto-Y: "Activin A:a commitment factor in erythroid differentiation" Biochem-Biophys-Res-Commun. 242. 631-635 (1998)
[文献書誌] Ozaki-S;Ogasahara-K et al: "Hepatosplenic gamma delta T-cell lymphoma associated with hepatitis B virus infection" J-Med-Invest.44. 215-217 (1998)
[文献書誌] Ozaki-S;Kosaka-M,et al: "Radioimmunodetection of human myeloma xenografts with a monoclonal antibody directed against a plasma cell specific antigen,HM1.24" Cancer.82. 2184-2190 (1998)
[文献書誌] Mengersen KL,Merrilces MJ: "Environmental tobacco smoke and ischemic heart disease:A case study in applying causal criteria." Int Arch of Occupational Environmental Health. (in press). (1999)
[文献書誌] Scott L,Mcrrilees M et al: "Subendothelial proteoglycan synthesis and transforming growth factor beta distribution correlate with susceptibility to atherosclerosis." J.Vasc.Res. 34. 365-377 (1997)
[文献書誌] Cornish-J;Callon-KE et al: "Systemic administration of amylin increases bone mass,lincar growth,and adiposity in adult malc mice." Am-J-Physiol.274. E694-699 (1998)
[文献書誌] Cornish-J;Callon-KE et al: "Dissociation of the effects of amylin on osteblast proliferation and bone resorption" Am-J-Physiol. 274. E827-833 (1998)
[文献書誌] Charles-CJ;Rademaker-MT et al: "Hemodynamic,hormonal,and renal effects of intracerebroventricular adrenomcdullin in conscious sheep" Endocrinology.137. 1746-1751 (1998)
[文献書誌] Philpott-M;Joseph-WR et al: "Production of tumour necrosis factor-alpha by cultured human peripheral blood leucocytesin response to the anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid(NSC 640488)" Br-J-Cancer. 76. 1586-1591 (1997)
[文献書誌] Crosier-KE;Crosier・PS: "New insights into the control of cell growth:the role of the Axl family." Pathology. 29. 131-135 (1970)
[文献書誌] Davidson-A & Freeman-SA et al: "Expression of murine interleukin II and its receptor alpha-chein in adult and embryonic tissues" Stem-Cells.15. 119-124 (1997)
[文献書誌] Becker,D.L.,McGonnell et al: "Roles for al connexin in morphogenesis of chick embryos recealed using a novel antisense approach" Devel.Genetics.in press (1999)
[文献書誌] Blackburn,J.P.,Connat.J-L et al: "Connexin43 gap junction levels during development of the thoracie zorte are temporzlly correlated with elastic laminae deposition and increased blood pressure" Cell Biol Int. 21. 87-97 (1997)
[文献書誌] Donaldson.P.,Eckert,R et al: "Gap Junctional channels:new roles in disease" Histol Histopathol. 12. 219-231 (1997)
[文献書誌] Green.C.R.: "In:Prthciples of Medical Biology,Volume II,Developmental Biology(Eds.E.E.Bitter and N Bitter)JAl Press Inc.,Green with Connecitcut." Gap Junctions.1993, 103-121 (1998)
[文献書誌] Eckert,R.,Donaldson.P et al: "Current Topics Membranes.Academic Press.ln press" Geting of gap junction channels and hermichannels in melers: a role in catarase?(in press), (1999)

1998 年度 実績報告書

細胞増殖因子、形態形成因子の細胞分化における役割

研究代表者

荒瀬 誠治 徳島大学, 医学部, 教授 (90108887)

研究成果

[文献書誌] Absan-MK.Urano-Y.et al: "Immunohistochemical localization of thyroid hormone nuclear receptors in human hair follicles and in vitro effect of L-triiodothyronine on cultured cells of hair follicles and skin" J-Med-Invest.44. 179-184 (1998)

[文献書誌] Matsumoto-K;Urano-Y et al: "Mutation of the fibroblast growth factor receptor 2 gene in Japanese patients with Apert syndrome." Plast-Reconstr-Surg. 101(2). 307-311 (1998)

[文献書誌] Kubo Y,Urano et al: "Lack of mutation in the INK4a locus in basal cell carcinoma." Br J Dermatol. 139. 340-341 (1998)

[文献書誌] Hamaoka-H;Minakuchi et al.: "Effect of K+ channel openers on K+ channcl in cultured human dermal papilla cells" J-Med-Invest. 44. 73-77 (1997)

[文献書誌] Moritani-M;Yoshimoto-K et al: "Abrogation of autoimmune diabetes in nonobese diabetic mice and protection against effector lymphocytes by transgenic paracrine TGF-betal" J-Clin-Invest.102. 499-506 (1998)

[文献書誌] Tanaka-C;Kimura-T et al: "Analysis of loss of heterozygosity on chromosome II and infrequent inactivation of the MEN1 gene in sporadic pituitary adcnomas[see comments]" J-Clin-Endocrinol-Metab. 83. 2631-2634 (1998)

[文献書誌] Kawano-Y;Takaue-Y.et al: "Partially mismatched pediatric transplants with allogeneic CD34(+) blood cells from a related donor" Blood. 92. 3123-3130 (1998)

[文献書誌] Abe-T;Makimoto-A et al: "Intra-apheresis recruitment of blood progenitor cells in children" Transfusion. 38. 944-950 (1998)

[文献書誌] Kawano-Y;Takaue-Y et al.: "Peripheral blood stem cell mobilization with granulocyte colony-stimulating factor and a harvesting procedure in pediatric donors" Bone-Marrow-Transplant. 21.S3. S32-34 (1998)

[文献書誌] Yamanaka-H;Takeda-E et al: "Total parenteral nutrition on energy metabolism in children undergoing autologous peripheral blood stem cell transplantation." J-Med-Invest.44. 199-203 (1998)

[文献書誌] Kajiume-T;Kawano-Y et al: "New consecutive high-dose chemotherapy modality with fractionated blood stem cell support in the treatment of high-risk pediatric solid tumors:a feasibility study" Bone-Marrow-Transplant. 21. 147-151 (1998)

[文献書誌] Merrilees,MJ,Beaumont B et al: "Inhibition of proteoglycan synthesis in balloon-injured rat and rabbit carotid arteries exposed to TGF-b1 antisense S-oligonucleotide." J.Vas.Res(In press). (in press). (1999)

[文献書誌] Hamaoka-T;Idehara-C et al: ":Hypoplasia of pancreatic islets in transgenic mice expressing activin receptor mutants" J-Clin-Invest. 102. 294-301 (1998)

[文献書誌] Tanaka-C;Yoshimoto-K,et al: "Absence of germ-line mutations of the multiple endocrine neoplasia type I(MEM1)gene in familial pituitary adenoma in contrast to MEN1 in Japanese" J-Clin-Endocrinol-Metab. 83. 960-965 (1998)

[文献書誌] Matsushita-T;Yamaoka-T,et al: "Molecular cloning of mouse paired-box-containing gene(Pax)-4 from an islet beta cell lineand deduced sequence of human Pax-4." Biochem-Biophys-Res-Commun. 242. 176-180 (1998)

[文献書誌] Shiozaki-M;Kosaka-M;Eto-Y: "Activin A:a commitment factor in erythroid differentiation" Biochem-Biophys-Res-Commun. 242. 631-635 (1998)

[文献書誌] Ozaki-S;Ogasahara-K et al: "Hepatosplenic gamma delta T-cell lymphoma associated with hepatitis B virus infection" J-Med-Invest.44. 215-217 (1998)

[文献書誌] Ozaki-S;Kosaka-M,et al: "Radioimmunodetection of human myeloma xenografts with a monoclonal antibody directed against a plasma cell specific antigen,HM1.24" Cancer.82. 2184-2190 (1998)

[文献書誌] Mengersen KL,Merrilces MJ: "Environmental tobacco smoke and ischemic heart disease:A case study in applying causal criteria." Int Arch of Occupational Environmental Health. (in press). (1999)

[文献書誌] Scott L,Mcrrilees M et al: "Subendothelial proteoglycan synthesis and transforming growth factor beta distribution correlate with susceptibility to atherosclerosis." J.Vasc.Res. 34. 365-377 (1997)

[文献書誌] Cornish-J;Callon-KE et al: "Systemic administration of amylin increases bone mass,lincar growth,and adiposity in adult malc mice." Am-J-Physiol.274. E694-699 (1998)

[文献書誌] Cornish-J;Callon-KE et al: "Dissociation of the effects of amylin on osteblast proliferation and bone resorption" Am-J-Physiol. 274. E827-833 (1998)

[文献書誌] Charles-CJ;Rademaker-MT et al: "Hemodynamic,hormonal,and renal effects of intracerebroventricular adrenomcdullin in conscious sheep" Endocrinology.137. 1746-1751 (1998)

[文献書誌] Philpott-M;Joseph-WR et al: "Production of tumour necrosis factor-alpha by cultured human peripheral blood leucocytesin response to the anti-tumour agent 5,6-dimethylxanthenone-4-acetic acid(NSC 640488)" Br-J-Cancer. 76. 1586-1591 (1997)

[文献書誌] Crosier-KE;Crosier・PS: "New insights into the control of cell growth:the role of the Axl family." Pathology. 29. 131-135 (1970)

[文献書誌] Davidson-A & Freeman-SA et al: "Expression of murine interleukin II and its receptor alpha-chein in adult and embryonic tissues" Stem-Cells.15. 119-124 (1997)

[文献書誌] Becker,D.L.,McGonnell et al: "Roles for al connexin in morphogenesis of chick embryos recealed using a novel antisense approach" Devel.Genetics.in press (1999)

[文献書誌] Blackburn,J.P.,Connat.J-L et al: "Connexin43 gap junction levels during development of the thoracie zorte are temporzlly correlated with elastic laminae deposition and increased blood pressure" Cell Biol Int. 21. 87-97 (1997)

[文献書誌] Donaldson.P.,Eckert,R et al: "Gap Junctional channels:new roles in disease" Histol Histopathol. 12. 219-231 (1997)

[文献書誌] Green.C.R.: "In:Prthciples of Medical Biology,Volume II,Developmental Biology(Eds.E.E.Bitter and N Bitter)JAl Press Inc.,Green with Connecitcut." Gap Junctions.1993, 103-121 (1998)

[文献書誌] Eckert,R.,Donaldson.P et al: "Current Topics Membranes.Academic Press.ln press" Geting of gap junction channels and hermichannels in melers: a role in catarase?(in press), (1999)

1998 年度実績報告書

荒瀬誠治徳島大学, 医学部, 教授 (90108887)