1998 年度実績報告書

細胞複製装置:染色体の複製・分配機構

研究課題

研究課題/領域番号	08277103
研究機関	熊本大学
研究代表者	平賀莊太熊本大学, 医学部, 教授 (40027321)
研究分担者	吉川寛奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (70019876) 柳田充弘京都大学, 大学院・理学研究所, 教授 (80025428) 伊藤嘉明京都大学, ウイルス研究所, 教授 (80004612) 滝澤温彦大阪大学, 大学院・理学研究科, 助教授 (60154944) 杉野明雄大阪大学, 微生物病研究所, 教授 (90231737)
キーワード	DNA複製 / 染色体分配 / 複製蛋白 / 細胞分裂 / 複製起点 / ライセンス因子
研究概要	1. 各班会議にて各研究班の進展状況を把握し、評価、助言を行った。班会議への経費の援助を行った。 3. 東京ガーデンパレスで開催された第4回公開シンポジウム「分子細胞生物学の新しい流れ」を「細胞核の機能構造」代表/水野重樹、「生殖細胞の特質」代表/山本正幸の3重点領域研究で共催し、福岡海の中道で開催された「第14回DNA複製ワークショップ」を九州大学・薬学部・関水和久と共催した。 4. 総括班の平賀壮太がアメリカで開催された米国微生物学会の「染色体分配」で研究成果発表及び、情報収集への経費を援助した。 5. サーキュラーを発行し、総括班・計画研究班・公募研究の班員間へワークショップやシンポジウムの連絡また報告等を行い連絡をはかった。 6. 今年度の計画研究班員及び、公募研究班員の研究成果をまとめ、研究成果報告書を作成し、各班員に配付した。

研究成果
(16件)

すべてその他

すべて文献書誌 (16件)

[文献書誌] Onogi,T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli." Mol.Microbiol.(in press). (1999)
[文献書誌] Hiraga,S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-assciated DNA-protein complexes in E.coli." Mol.Cell.1. 381-387 (1998)
[文献書誌] Hirano,M.: "Autoregulation of the partition genes of mini-F plasmid and the intracellilar localization of their products in Escherichia coli." Mol.Gen.Genet.257. 392-403 (1998)
[文献書誌] Niki,H.: "Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning." Genes&Development. 12. 1036-1045 (1998)
[文献書誌] Sugino,A.: "DNA polymerase epsilon encoded by cdc20^+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe." Genes Cells. 3. 99-110 (1998)
[文献書誌] Noskov,V.N: "The RFC2 gene,encoding the third-largest subunit of the replication factor C complex,is required for an S-phase checkpoint in saccharomyces cervisiae." Mol Cell Biol. 18. 4914-4923 (1998)
[文献書誌] Maki,S.: "DNA polymerase II(epsilon)of Saccharomyces cerevisiae dissociates from the DNA template by sensing single-stranded DNA." J Biol Chem. 273. 21332-21341 (1999)
[文献書誌] Kamimura,Y.: "Sld2,which interacts with Dpb11 in Saccharomyces cerevisiae,is required for chromosomal DNA replication." Moi Cell Biol. 18. 6102-6109 (1998)
[文献書誌] Kamimura,YI: "DNA helicase III of Saccharomyces cereviase,encoded by YER176w(HEL1),highly unwinds covalently closed,circular DNA in the presence of a DNA topoismoerase and yRF-A." J Biochem. 125. 236-244 (1999)
[文献書誌] 0hno,K.: "cDNA cloning and expression during development of Drosophila melanogaster MCM3,MCM6 and MCM7." Gene. 217. 177-185 (1998)
[文献書誌] Takahashi,Y.: "Identification of three conserved regions in the DREF transcription factors from drosophila melanogaster and drosophila virilis." Nucleic Acids Res. 27. 510-516 (1999)
[文献書誌] Sawado,T.: "The DNA replication-related element(DRE)/DRE-binding factor system is a transcriptional regulator of the Drosophila E2F gene." J Biol Chem. 273. 26042-26051 (1998)
[文献書誌] Sawado,T.: "dE2F,a novel E2F-family transcription factor in Drosophila melanogaster." Biochem.Biophys.RES.Commun.251. 409-451 (1998)
[文献書誌] Mimura,S.: "Xenoppus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-shape cdk." EMBOJ. 17. 5699-5707 (1998)
[文献書誌] 滝沢温彦: "MCM/P1ファミリータンパク質とDNA複製のライセンス化" 実験医学. 16. 997-1002 (1998)
[文献書誌] Mizushima,Y.: "Studies on inhibitors of mammalian DNA polymerase α and β,Sulfolipids from a pteridophyte,Athyrium miponicum." Biochem.Pharmacol.55. 537-541 (1998)

1998 年度 実績報告書

細胞複製装置:染色体の複製・分配機構

研究代表者

平賀 莊太 熊本大学, 医学部, 教授 (40027321)

研究成果

[文献書誌] Onogi,T.: "The assembly and migration of SeqA-Gfp fusion in living cells of Escherichia coli." Mol.Microbiol.(in press). (1999)

[文献書誌] Hiraga,S.: "Cell cycle-dependent duplication and bidirectional migration of SeqA-assciated DNA-protein complexes in E.coli." Mol.Cell.1. 381-387 (1998)

[文献書誌] Hirano,M.: "Autoregulation of the partition genes of mini-F plasmid and the intracellilar localization of their products in Escherichia coli." Mol.Gen.Genet.257. 392-403 (1998)

[文献書誌] Niki,H.: "Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning." Genes&Development. 12. 1036-1045 (1998)

[文献書誌] Sugino,A.: "DNA polymerase epsilon encoded by cdc20^+ is required for chromosomal DNA replication in the fission yeast Schizosaccharomyces pombe." Genes Cells. 3. 99-110 (1998)

[文献書誌] Noskov,V.N: "The RFC2 gene,encoding the third-largest subunit of the replication factor C complex,is required for an S-phase checkpoint in saccharomyces cervisiae." Mol Cell Biol. 18. 4914-4923 (1998)

[文献書誌] Maki,S.: "DNA polymerase II(epsilon)of Saccharomyces cerevisiae dissociates from the DNA template by sensing single-stranded DNA." J Biol Chem. 273. 21332-21341 (1999)

[文献書誌] Kamimura,Y.: "Sld2,which interacts with Dpb11 in Saccharomyces cerevisiae,is required for chromosomal DNA replication." Moi Cell Biol. 18. 6102-6109 (1998)

[文献書誌] Kamimura,YI: "DNA helicase III of Saccharomyces cereviase,encoded by YER176w(HEL1),highly unwinds covalently closed,circular DNA in the presence of a DNA topoismoerase and yRF-A." J Biochem. 125. 236-244 (1999)

[文献書誌] 0hno,K.: "cDNA cloning and expression during development of Drosophila melanogaster MCM3,MCM6 and MCM7." Gene. 217. 177-185 (1998)

[文献書誌] Takahashi,Y.: "Identification of three conserved regions in the DREF transcription factors from drosophila melanogaster and drosophila virilis." Nucleic Acids Res. 27. 510-516 (1999)

[文献書誌] Sawado,T.: "The DNA replication-related element(DRE)/DRE-binding factor system is a transcriptional regulator of the Drosophila E2F gene." J Biol Chem. 273. 26042-26051 (1998)

[文献書誌] Sawado,T.: "dE2F,a novel E2F-family transcription factor in Drosophila melanogaster." Biochem.Biophys.RES.Commun.251. 409-451 (1998)

[文献書誌] Mimura,S.: "Xenoppus Cdc45-dependent loading of DNA polymerase alpha onto chromatin under the control of S-shape cdk." EMBOJ. 17. 5699-5707 (1998)

[文献書誌] 滝沢温彦: "MCM/P1ファミリータンパク質とDNA複製のライセンス化" 実験医学. 16. 997-1002 (1998)

[文献書誌] Mizushima,Y.: "Studies on inhibitors of mammalian DNA polymerase α and β,Sulfolipids from a pteridophyte,Athyrium miponicum." Biochem.Pharmacol.55. 537-541 (1998)

1998 年度実績報告書

平賀莊太熊本大学, 医学部, 教授 (40027321)