1997 年度実績報告書

ゲノム機能解析

研究課題

研究課題/領域番号	08283105
研究機関	国立遺伝学研究所
研究代表者	小原雄治国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)
研究分担者	加藤誠志 (財)相模中央化学研究所, 主任研究員小笠原直毅奈良先端科学技術大学院大学, 教授 (10110553) 村上康文理化学研究所, 筑波研究センター, 先任研究員 (90200279) 伊藤隆司東京大学, 医科学研究所, 助手 (90201326) 大久保公策大阪大学, 細胞生体工学センター, 助教授 (40233069)
キーワード	ボディマップ / cDNA / 遺伝子破壊 / 遺伝子発現パターン / 線虫 / 出芽酵母 / 枯草菌 / ディファレンシャルディスプレイ
研究概要	成果報告ヒトcDNA解析については、まずヒト各組織54およびマウス主要臓器から3'無作為シーケンスを約110,000集積しボディマップの充実を進めた(大久保)。また完全長cDNAの解析を進め1260種類の新規蛋白質遺伝子を含む2130種類の完全長cDNAクローンを同定し、200種類の新規完全長の配列決定をした(加藤)。未知遺伝子の機能解析のために酵母、マウスを用いた遺伝子破壊システムの確立をおこなった(高橋)。モデル生物ゲノムの解析では、線虫ゲノムのcDNAのカタログ化を全遺伝子の半数以上の8000種にまで進め、約2000種について発現細胞(系譜)を決定し発現パターンマップの構築を進めた(小原)。細胞性粘菌のcDNA解析を進めるため、18,000クローンをグリッド化し、タグ塩基配列決定、アンチセンスRNA発現コンストラクト作成を進めた(前田)。出芽酵母ゲノムでは第6染色体の遺伝子破壊実験から見いだされた24個の増殖必遺伝子について表現型解析をおこなった(村上)。EUグループとの共同で野生株、破壊株、過剰産生株の間でFDD解析を行い、新規の標的遺伝子候補22種及び8種の遺伝子を負の標的遺伝子として同定した。酵母全ORFの間でtwo-hybrid解析による蛋白質相互作用解析の準備をした(伊藤)。枯草菌ゲノムではわが国で決定された14Mb領域の約1400の遺伝子のうち443の遺伝子の破壊変異株を作成した。表現型のシステマチックな検出のために、7種類のC源の利用能、カタボライト感受性、蛋白質の分泌能、細胞の運動性、DNA損傷の感受性、塩・エタノール・過酸化水素・温度などのストレスに対する感受性、SDSに対する感受性を調べるための21種類のプレートを選び、コロニーの大きさ(増殖能)とLacZ活性による呈色の変化(発現)を調べる作業を開始した。また全蛋白の2次元電気泳動での同定と破壊株との対応付けを進めた(小笠原)。以上の研究結果は東大医科研ヒトゲノムセンターなどにおいてインターネット上での公開をおこなっている。

研究成果

(47件)

すべてその他

すべて文献書誌 (47件)

[文献書誌] Kunst,F: "The complete genome sequence of the gram-positive bacterium Bacillus subtilis" Nature. 390. 249-256 (1997)
[文献書誌] Kasahara,Y: "Sequence analysis of the groESL-cotA region of the Bacillus Subtilis genome,containing the restriction/modification system gene" DNA Res.4. 335-339 (1997)
[文献書誌] Beloin,C.: "Characterization of alrp-like gene in Bacillus subtilis." Biol.Gen.Genet. 256. 63-71 (1997)
[文献書誌] Sadaie,Y.: "Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome." Microbiology. 143. 1861-1866 (1997)
[文献書誌] Kasahara,Y.: "Sequence analysis of the 36Kb region between gntZ and tmY genes of Bacillus subtilis genome." DNA Res.4. 155-159 (1997)
[文献書誌] Kito,K: "Fluorescent differential display analysis of gene expression in differentiating neuroblastoma cells" Gene. 184. 73-81 (1997)
[文献書誌] Hata,K.: "Anionic amphiphile-independent activation of the phagocyte NADPH oxidase in a cell-free system by p47phox and p67phox,both in C terminally truncated forms:lmplication for regulatory SH3 domain-mediated interactions" J.Biol.Chem.273. 4232-4236 (1998)
[文献書誌] Kato,K.: "Adaptor-tagged competitive PCR: a novel m ethod for measuring relative gene expression." Nucleic Acids Research. 25. 4694-4696 (1997)
[文献書誌] Yoshioka,S.: "Identification of open reading frames in Schizosaccharomyces pombe cDNAs." DNA Research. 4. 363-369 (1997)
[文献書誌] Shimizu,F.: "Isolation of a novel human cDNA (rho HP1) homologous to rho genes." Biochemica et Biophysica Acta. 1351. 13-16 (1997)
[文献書誌] Takahashi,N.: "Molecular propaties of the proteasome activator PA28 family proteins and gamma-interferon regulation." Genes to Cells. 2. 195-211 (1997)
[文献書誌] Watanabe,K.T.: "Cloning and characterization of a novel member of the human Mad gene family (MADH)." Genomics. 42. 446-451 (1997)
[文献書誌] Maeda K: "Analysis of an expression profile of genes in the human adipose tissue" Gene. 190. 227-235 (1997)
[文献書誌] Okubo K: "Complementary DNA sequence(EST)collections and the expression information of the human genome" FFBS letters. 403. 225-229 (1997)
[文献書誌] Matsumoto A: "An expression profile of Genes in Human Retina and Isolation of a cDNA for a Novel Rod Photoreceptor protein" IOVS. 38. 2576-2585 (1997)
[文献書誌] Kuriyama H: "Molecular cloning and Expression of a Novel Aquaporin from Adipose Tissue with Glycerol permeabllity" BBRC. 241. 53-58 (1997)
[文献書誌] Matsuo N: "Cloning of a cDNA encoding a novel sugar transporter expressed in the neonatal mouse hippocampus" BBRC. 238. 126-129 (1997)
[文献書誌] Blazejewski S: "Expression in human liver and cultured human liver myofibroblasts" Am.j.of Pathology. 151. 651-657 (1997)
[文献書誌] Daigo,Y.: "Isolation, mapping and mutation analysis of a haman cDNA homologous to the doc-1 gene of the chinese hamster, a candidate tumor supressor for oral cancer." Genes, Chromosomes and Cancer. 20. 204-207 (1997)
[文献書誌] Takei,Y.: "Molecular cloning of a novel gene similar to myeroid antigen CD33 and its specific expression in placenta." Cytogenet.Cell Genet.78. 295-300 (1997)
[文献書誌] Omori,Y.: "Cloning and mapping of a novel human zinc-finger gene TCF-17 homologous to rodent kid1." Cytogenet.Cell Genet.78. 285-288 (1997)
[文献書誌] Onda,M.: "Expression and chromosomal localization of the human alpha 4/1GBP1 gene, the structure of which is closely related to the yeast TAR42 protein of the rapamycin-sensitive signal transduction pathway." Genomics. 46. 373-378 (1997)
[文献書誌] Hill,A.: "Assignment of fragile site 8E(FRA8E) of human chromosome band 8q24.11 adjacent to the hereditary multiple exostoses 1 gene and two overlapping Langer-Giedeon syndrome deletion endpoints." Cytogenet. Cell Genet.78. 56-57 (1997)
[文献書誌] Eki,T.: "Cloning and characterization of novel gene, DCRR1, expressed from Down's syndrome critical region of human chromosome 21q22.2" DNA Sequence. 7. 153-164 (1997)
[文献書誌] Ohira,M.: "Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21" Genome Res.7. 47-58 (1997)
[文献書誌] Naitou,M.: "Expression profiles of transcripts from 126 ORFs in Saccharomyces cerevisiae chromosome VI by systematic Northern analyse" Yeast. (in press). (1997)
[文献書誌] Ishiai,M.: "Isolation of human and fission yeast gene homologues of the budding yeast origin recognition complex subunit Orc5:Human homologue (PRC5L) maps to chromosome band 7q22" Genomics. (in press).
[文献書誌] Eki,T.: "Mapping of the human genes encoding cyclin H (CCNH) and CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively," Genomics. (in press).
[文献書誌] Goffeau.A.: "Yeast Genome Directory" Nature. Supplement to Vol 387,29 May. (1997)
[文献書誌] Sadamoto,A.: "Both hypertrophic and dilated cardiomyopathies are caused by mutation of the same gene, delta-sarcoglycan, in hamster:An animalmodel of disrupted dystrophin-associated glycoprotein complex" Proc.Nati.Acad.Sci.USA. 94. 13873-13878 (1997)
[文献書誌] Nakazato,A: "Comparison of the structural characteristics of chromosome VI in Saccharomyces sensu stricto:The divergence, species-dependent features and uniqueness of Sake yeast" Yeast. (in press). (1998)
[文献書誌] 村上康文: "ヒト遺伝子全容の理解を目指して:その1 ゲノム解析研究の目的とその方法" 科学技術ジャーナル. 1月号. 66-69 (1998)
[文献書誌] 村上康文: "ヒト遺伝子全容の理解を目指して:その2 ゲノム解析研究の目的とその方法" 科学技術ジャーナル. 2月号. 66-69 (1998)
[文献書誌] 村上康文: "ヒト遺伝子全容の理解を目指して:その3 塩基配列の解読法" 科学技術ジャーナル. 3月号. 66-69 (1998)
[文献書誌] 村上康文: "酵母:全ての真核生物のモデルとして" サイアス. 1998年2月20日号. 53 (1998)
[文献書誌] 村上康文: "出芽酵母のゲノム解析" 蛋白質核酸酵素. 42巻(17). 2927-2932 (1997)
[文献書誌] Furihata,C.: "Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N'-nitro-N nitrosoguanidine exposure" Cancer Res.57. 1416-1418 (1997)
[文献書誌] Furihata,C.: "Rare occurrence of ras and p53 gene mutation in mouse stomach tumors induced by N-methyl-N-nitrosourea" Jpn.J.Cancer Res.88. 363-368 (1997)
[文献書誌] Hagiwara,Y.: "Screening for imprinted genes by allelic message display:Identification of a patemally expressed gene Impact on mouse chromosome 18" Proc.Natl.Acad.Sci.USA. 94. 9249-9254 (1997)
[文献書誌] Adati,N: "Isolation and expression study of a maternally expressed novel Xenopus gene Xem1 encoding a putative evolutionarily conserved membrane protein" Biochem.Biophys.Res.Commun. 238. 899-904 (1997)
[文献書誌] Mizuki,K.: "Functional modules and expression of mouse p40phox and p67phox, Src homology 3 (SH3) domain-containing proteins involved in the phagocyte NADPH oxidase complex" Eur.J.Biochem.251. 573-582 (1998)
[文献書誌] Nakamura,R.: "The PC motif:A novel and evolutionarily conserved sequence involved in interaction between p40phox and p67phox,SH3 domain-containing cytosolic factors of the phagocyte NADPH oxidase" Eur.J.Biochem.251. 583-589 (1998)
[文献書誌] 村上康文: "酵母の機能解析プロジェクト" 蛋白質核酸酵素. 42巻(17). 3067-3069 (1997)
[文献書誌] M.Maeda: "Activation of the mitogen-activated protein kinase ERK2 by the chemoattractant folic acid in Dictyostelium." J.Biol.Chem.vol.272(38). 23690-23695 (1997)
[文献書誌] Y.Kubohara: "Effient induction by DIF-1 and 8-bromo cyclic AMP of prespore-to-stalk conversion in Dictyostelium discoideum." Com.Biochem.Physiol.118A(3). 841-845 (1997)
[文献書誌] L.Aubry: "The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by ras and cAMP-dependent protein kinase (PKA) and mediates PKA function." J.Bio.Chem.vol.272(7). 3883-3886 (1997)
[文献書誌] 小原雄治: "線虫(1000細胞のシンフォニ-)" 共立出版株式会社, 1-153 (1997)

1997 年度 実績報告書

ゲノム機能解析

研究代表者

小原 雄治 国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)

研究成果

[文献書誌] Kunst,F: "The complete genome sequence of the gram-positive bacterium Bacillus subtilis" Nature. 390. 249-256 (1997)

[文献書誌] Kasahara,Y: "Sequence analysis of the groESL-cotA region of the Bacillus Subtilis genome,containing the restriction/modification system gene" DNA Res.4. 335-339 (1997)

[文献書誌] Beloin,C.: "Characterization of alrp-like gene in Bacillus subtilis." Biol.Gen.Genet. 256. 63-71 (1997)

[文献書誌] Sadaie,Y.: "Nucleotide sequence and analysis of the phoB-rrnE-groESL region of the Bacillus subtilis chromosome." Microbiology. 143. 1861-1866 (1997)

[文献書誌] Kasahara,Y.: "Sequence analysis of the 36Kb region between gntZ and tmY genes of Bacillus subtilis genome." DNA Res.4. 155-159 (1997)

[文献書誌] Kito,K: "Fluorescent differential display analysis of gene expression in differentiating neuroblastoma cells" Gene. 184. 73-81 (1997)

[文献書誌] Hata,K.: "Anionic amphiphile-independent activation of the phagocyte NADPH oxidase in a cell-free system by p47phox and p67phox,both in C terminally truncated forms:lmplication for regulatory SH3 domain-mediated interactions" J.Biol.Chem.273. 4232-4236 (1998)

[文献書誌] Kato,K.: "Adaptor-tagged competitive PCR: a novel m ethod for measuring relative gene expression." Nucleic Acids Research. 25. 4694-4696 (1997)

[文献書誌] Yoshioka,S.: "Identification of open reading frames in Schizosaccharomyces pombe cDNAs." DNA Research. 4. 363-369 (1997)

[文献書誌] Shimizu,F.: "Isolation of a novel human cDNA (rho HP1) homologous to rho genes." Biochemica et Biophysica Acta. 1351. 13-16 (1997)

[文献書誌] Takahashi,N.: "Molecular propaties of the proteasome activator PA28 family proteins and gamma-interferon regulation." Genes to Cells. 2. 195-211 (1997)

[文献書誌] Watanabe,K.T.: "Cloning and characterization of a novel member of the human Mad gene family (MADH)." Genomics. 42. 446-451 (1997)

[文献書誌] Maeda K: "Analysis of an expression profile of genes in the human adipose tissue" Gene. 190. 227-235 (1997)

[文献書誌] Okubo K: "Complementary DNA sequence(EST)collections and the expression information of the human genome" FFBS letters. 403. 225-229 (1997)

[文献書誌] Matsumoto A: "An expression profile of Genes in Human Retina and Isolation of a cDNA for a Novel Rod Photoreceptor protein" IOVS. 38. 2576-2585 (1997)

[文献書誌] Kuriyama H: "Molecular cloning and Expression of a Novel Aquaporin from Adipose Tissue with Glycerol permeabllity" BBRC. 241. 53-58 (1997)

[文献書誌] Matsuo N: "Cloning of a cDNA encoding a novel sugar transporter expressed in the neonatal mouse hippocampus" BBRC. 238. 126-129 (1997)

[文献書誌] Blazejewski S: "Expression in human liver and cultured human liver myofibroblasts" Am.j.of Pathology. 151. 651-657 (1997)

[文献書誌] Daigo,Y.: "Isolation, mapping and mutation analysis of a haman cDNA homologous to the doc-1 gene of the chinese hamster, a candidate tumor supressor for oral cancer." Genes, Chromosomes and Cancer. 20. 204-207 (1997)

[文献書誌] Takei,Y.: "Molecular cloning of a novel gene similar to myeroid antigen CD33 and its specific expression in placenta." Cytogenet.Cell Genet.78. 295-300 (1997)

[文献書誌] Omori,Y.: "Cloning and mapping of a novel human zinc-finger gene TCF-17 homologous to rodent kid1." Cytogenet.Cell Genet.78. 285-288 (1997)

[文献書誌] Onda,M.: "Expression and chromosomal localization of the human alpha 4/1GBP1 gene, the structure of which is closely related to the yeast TAR42 protein of the rapamycin-sensitive signal transduction pathway." Genomics. 46. 373-378 (1997)

[文献書誌] Hill,A.: "Assignment of fragile site 8E(FRA8E) of human chromosome band 8q24.11 adjacent to the hereditary multiple exostoses 1 gene and two overlapping Langer-Giedeon syndrome deletion endpoints." Cytogenet. Cell Genet.78. 56-57 (1997)

[文献書誌] Eki,T.: "Cloning and characterization of novel gene, DCRR1, expressed from Down's syndrome critical region of human chromosome 21q22.2" DNA Sequence. 7. 153-164 (1997)

[文献書誌] Ohira,M.: "Gene identification in 1.6-Mb region of the Down syndrome region on chromosome 21" Genome Res.7. 47-58 (1997)

[文献書誌] Naitou,M.: "Expression profiles of transcripts from 126 ORFs in Saccharomyces cerevisiae chromosome VI by systematic Northern analyse" Yeast. (in press). (1997)

[文献書誌] Ishiai,M.: "Isolation of human and fission yeast gene homologues of the budding yeast origin recognition complex subunit Orc5:Human homologue (PRC5L) maps to chromosome band 7q22" Genomics. (in press).

[文献書誌] Eki,T.: "Mapping of the human genes encoding cyclin H (CCNH) and CDK-activating kinase (CAK) assembly factor MAT1 (MNAT1) to chromosome bands 5q13.3-q14 and 14q23, respectively," Genomics. (in press).

[文献書誌] Goffeau.A.: "Yeast Genome Directory" Nature. Supplement to Vol 387,29 May. (1997)

[文献書誌] Sadamoto,A.: "Both hypertrophic and dilated cardiomyopathies are caused by mutation of the same gene, delta-sarcoglycan, in hamster:An animalmodel of disrupted dystrophin-associated glycoprotein complex" Proc.Nati.Acad.Sci.USA. 94. 13873-13878 (1997)

[文献書誌] Nakazato,A: "Comparison of the structural characteristics of chromosome VI in Saccharomyces sensu stricto:The divergence, species-dependent features and uniqueness of Sake yeast" Yeast. (in press). (1998)

[文献書誌] 村上康文: "ヒト遺伝子全容の理解を目指して:その1 ゲノム解析研究の目的とその方法" 科学技術ジャーナル. 1月号. 66-69 (1998)

[文献書誌] 村上康文: "ヒト遺伝子全容の理解を目指して:その2 ゲノム解析研究の目的とその方法" 科学技術ジャーナル. 2月号. 66-69 (1998)

[文献書誌] 村上康文: "ヒト遺伝子全容の理解を目指して:その3 塩基配列の解読法" 科学技術ジャーナル. 3月号. 66-69 (1998)

[文献書誌] 村上康文: "酵母:全ての真核生物のモデルとして" サイアス. 1998年2月20日号. 53 (1998)

[文献書誌] 村上康文: "出芽酵母のゲノム解析" 蛋白質核酸酵素. 42巻(17). 2927-2932 (1997)

[文献書誌] Furihata,C.: "Differentially expressed MHC class II-associated invariant chain in rat stomach pyloric mucosa with N-methyl-N'-nitro-N nitrosoguanidine exposure" Cancer Res.57. 1416-1418 (1997)

[文献書誌] Furihata,C.: "Rare occurrence of ras and p53 gene mutation in mouse stomach tumors induced by N-methyl-N-nitrosourea" Jpn.J.Cancer Res.88. 363-368 (1997)

[文献書誌] Hagiwara,Y.: "Screening for imprinted genes by allelic message display:Identification of a patemally expressed gene Impact on mouse chromosome 18" Proc.Natl.Acad.Sci.USA. 94. 9249-9254 (1997)

[文献書誌] Adati,N: "Isolation and expression study of a maternally expressed novel Xenopus gene Xem1 encoding a putative evolutionarily conserved membrane protein" Biochem.Biophys.Res.Commun. 238. 899-904 (1997)

[文献書誌] Mizuki,K.: "Functional modules and expression of mouse p40phox and p67phox, Src homology 3 (SH3) domain-containing proteins involved in the phagocyte NADPH oxidase complex" Eur.J.Biochem.251. 573-582 (1998)

[文献書誌] Nakamura,R.: "The PC motif:A novel and evolutionarily conserved sequence involved in interaction between p40phox and p67phox,SH3 domain-containing cytosolic factors of the phagocyte NADPH oxidase" Eur.J.Biochem.251. 583-589 (1998)

[文献書誌] 村上康文: "酵母の機能解析プロジェクト" 蛋白質核酸酵素. 42巻(17). 3067-3069 (1997)

[文献書誌] M.Maeda: "Activation of the mitogen-activated protein kinase ERK2 by the chemoattractant folic acid in Dictyostelium." J.Biol.Chem.vol.272(38). 23690-23695 (1997)

[文献書誌] Y.Kubohara: "Effient induction by DIF-1 and 8-bromo cyclic AMP of prespore-to-stalk conversion in Dictyostelium discoideum." Com.Biochem.Physiol.118A(3). 841-845 (1997)

[文献書誌] L.Aubry: "The Dictyostelium mitogen-activated protein kinase ERK2 is regulated by ras and cAMP-dependent protein kinase (PKA) and mediates PKA function." J.Bio.Chem.vol.272(7). 3883-3886 (1997)

[文献書誌] 小原雄治: "線虫(1000細胞のシンフォニ-)" 共立出版株式会社, 1-153 (1997)

1997 年度実績報告書

小原雄治国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)