1999 年度実績報告書

ゲノム機能解析

研究課題

研究課題/領域番号	08283105
研究機関	国立遺伝学研究所
研究代表者	小原雄治国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)
研究分担者	伊藤隆司金沢大学, がん研究所, 教授 (90201326) 菅野純夫東京大学, 医科学研究所, 助教授 (60162848) 大久保公策大阪大学, 細胞生体工学センター, 助教授 (40233069) 加藤菊也奈良先端科学技術大学院大学, 助教授 (60194809) 小笠原直毅奈良先端科学技術大学院大学, 教授 (10110553)
キーワード	遺伝子発現プロファイル / EST / RNAi / 完全長cDNA / 発現パターン / 表現型 / 遺伝子破壊 / タンパク間相互作用
研究概要	大久保公策 : 遺伝子発現プロファイル法(ATAC,iAFLP)をさらに改良した(3色蛍光使用でゲルあたり3456データポイントの確立やホルマリン固定病理検体からのRNAの利用)。ボディーマップを公開した。加藤菊也 : ATAC法を用いてマウス小脳の生後発生過程の6時点について2000個の遺伝子発現強度を測定し、遺伝子機能と関連づける解析方法を開発した。菅野純夫 : オリゴキャッピング法を用いて5種類の臓器より作製した完全長cDNAライブラリーから各約5000クローンの5'端を1pass塩基配列決定し、mRNAの開始点を体系的に明らかにした。加藤誠志 : 1,309種類のヒト完全長cDNAクローンを収集し、277種類の全長塩基配列を決定し、120遺伝子についてGFP融合遺伝子を利用して細胞内局在部位を決定した。高橋永一 : DD法にてマウスの201個の組織特異的遺伝子断片を単離し、機能解析を行った。小原雄治 : 線虫cDNA約7000種についてin situ hybridizationによる発現パターン解析をおこない、NEXTDBとして公開を始めた。母性発現を示すもの約300遺伝子についてRNAiによる機能解析を体系的におこなった。前田ミネ子 : 細胞性粘菌cDNA18000クローンのタグ塩基配列解析を終了した。発現パターン解析も進めた。伊藤隆司 : 出芽酵母全蛋白質間での網羅的2ハイブリッド解析をおこない、多数の新規相互作用とネットワークを同定した。村上康文 : 出芽酵母第VI染色体遺伝子の遺伝子発現プロフィールの解析を実施した。小笠原直毅 : 枯草菌893新規遺伝子の変異株バンクの発現、表現型解析を修了し、BSORFDB上で一般に公開した。

研究成果

(33件)

すべてその他

すべて文献書誌 (33件)

[文献書誌] Kawamoto S: "Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling"Genome Res.. 9(12). 1305-12 (1999)
[文献書誌] Hishiki T: "BodyMap: a human and mouse gene expression database"Nucleic Acids Res.. 28(1). 136-8 (2000)
[文献書誌] Ohnishi T: "Isolation of pure human mucosal epithelium for RNA analysis"Biotechniques. Nov;27(5). 978-80 (1999)
[文献書誌] Masuda N: "Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples"Nucleic Acids Res.. 27(22). 4436-43 (1999)
[文献書誌] Matoba, R.: "Gene expression in mouse cerebellum during its development"Gene,. 241. 125-131 (2000)
[文献書誌] Matoba, R.: "Correlation between gene functions and developmental expression patterns in the mouse cerebellum"Eur J. Neurosci. (in press).
[文献書誌] Shibui, A.,: "Isolation and chromosomal mapping of human homolog of intestinal Na+/PO4 cotransporter"J. Hum. Genet.. 44. 190-192 (1999)
[文献書誌] Tasaki, Y.: "Chemokine PARC gene (SCY18) generated by fusion of two MIP-1/LD78-like genes"Genomics. 55. 353-357 (1999)
[文献書誌] Ito, E.,: "Two short sequences have positive effects on the human p27 kip1 gene transcription"Gene. 228. 93-100 (1999)
[文献書誌] Yoshida, K,: "cDNA cloning and chromosomal mapping of mouse BH-protocadherin"DNA sequence. 10. 43-47 (1999)
[文献書誌] Yokoyama-Kobayashi, M.,: "Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank"Gene. 228. 161-167 (1999)
[文献書誌] Ito, K.,: "cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding and N-terminally extended human ubiquitin-conjugating enzyme"Cytogenet. Cell Genet. 84. 99-104 (1999)
[文献書誌] Komuro, A.,: "Npw38, a novel nuclear protein possessing a WW domain capable of activating basal transcription"Nucl. Acids Res.. 27. 1957-1965 (1999)
[文献書誌] Iwamuro, S.: "Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte Iysate"J. Biochem.. 126. 48-53 (1999)
[文献書誌] Nunoi, H.,: "A heterozygous mutation of beta-actin associated with neutrophil dysfunction and recurrent infection"Proc. Natl. Acad. Sci. USA. 96. 8693-8698 (1999)
[文献書誌] Komuro, A.: "Association of two nuclear proteins, Npw38 and NpwBP, via the interaction between the WW domain and a novel proline-rich motif containing glycine and arginine"J. Biol. Chem.. 274. 36513-36519 (1999)
[文献書誌] Fujiwara K.: "Molecular cloning, structural characterization and chromosomal localization of human lipolytransferase gene"Eur J Biochem. 260. 761-767 (1999)
[文献書誌] Harada Y.: "Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabiditis elegans sel-1"J. Hum Genet. 44. 330-336 (1999)
[文献書誌] Horie M.: "Cloning, expression and chromosomal napping of the human 14-3-3-gamma gene (YWHAG) to 7q11.23"Genomics. 60. 241-243 (1999)
[文献書誌] Hosokawa Y: "Human Aiolos, an Ikaros-related zinc finger DNA binding protein: cDNA cloning, tissue expression pattern, and chromosomal mapping"Genomics. 61. 326-329 (1999)
[文献書誌] Suzuki M.,: "Molecular cloning, expression, and mapping of a novel human cDNA, GRP17, highly homologous to human gadd45 and murine MyD118"J Hum Genet. 44. 300-303 (1999)
[文献書誌] Morohoshi F.: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1"Gene. 241. 287-295 (2000)
[文献書誌] Tabara, H.: "Pos-1 encodes a cytoplasmic zinc-finger protein essential for germline specification in C. elegans"Development. 126. 1-11 (1999)
[文献書誌] Fields, S.: "Functional Genomics"Proc. Natl. Acad. Sci. USA. 96. 8825-8826 (1999)
[文献書誌] Motii, M.,: "Identification of TGF-b-regulated genes in Caenorhabditis elegans by differential hybridization of arryaed cDNAs"Proc. Natl. Acad. Sci. USA. 96. 15020-15025 (1999)
[文献書誌] Cassata, C.,: "The Lim Homeobox Gene ceh-14 Confers Thermosensory Function to the AFD Neurons in Caenorhabditis elegans"Neuron. (in press).
[文献書誌] M. Maeda,: "Developmental changes in spatial expression of genes involved in myosin function of Dictyostelium"Dev. Biol.. (in press).
[文献書誌] Ito,. T.: "Toward a protein-protein interaction map of the budding yeast: A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins"Proc. Natl. Acad. Sci. USA. 97. 1143-1147 (1999)
[文献書誌] Shiratori, A.,: "Systematic Identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and northern analysis"Yeast. 15. 219-253 (1999)
[文献書誌] Oda, T.,: "A novel TATA-binding protein-binding protein, ABT1, activates basal transcription and has yeast homolog that is essential for growth"Mol. Cell. Biol. 20. 1407-1418 (2000)
[文献書誌] Ogasawara, N.: "Systematic function analysis of Bacillus subtilis genes"Res Microbiol. (in press).
[文献書誌] Fukuchi, K.,: "The essential two-component regulatory system encoded by yycF and yycG genes modulates expression of the ftsAZ operon in Bacillus subtilis"Microbiology. (in press).
[文献書誌] Asai, K.,: "Regulation of the transport system for C(4)-dicarboxylic acids in Bacillus subtilis"Microbiology. 146. 263-271 (2000)

1999 年度 実績報告書

ゲノム機能解析

研究代表者

小原 雄治 国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)

研究成果

[文献書誌] Kawamoto S: "Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling"Genome Res.. 9(12). 1305-12 (1999)

[文献書誌] Hishiki T: "BodyMap: a human and mouse gene expression database"Nucleic Acids Res.. 28(1). 136-8 (2000)

[文献書誌] Ohnishi T: "Isolation of pure human mucosal epithelium for RNA analysis"Biotechniques. Nov;27(5). 978-80 (1999)

[文献書誌] Masuda N: "Analysis of chemical modification of RNA from formalin-fixed samples and optimization of molecular biology applications for such samples"Nucleic Acids Res.. 27(22). 4436-43 (1999)

[文献書誌] Matoba, R.: "Gene expression in mouse cerebellum during its development"Gene,. 241. 125-131 (2000)

[文献書誌] Matoba, R.: "Correlation between gene functions and developmental expression patterns in the mouse cerebellum"Eur J. Neurosci. (in press).

[文献書誌] Shibui, A.,: "Isolation and chromosomal mapping of human homolog of intestinal Na+/PO4 cotransporter"J. Hum. Genet.. 44. 190-192 (1999)

[文献書誌] Tasaki, Y.: "Chemokine PARC gene (SCY18) generated by fusion of two MIP-1/LD78-like genes"Genomics. 55. 353-357 (1999)

[文献書誌] Ito, E.,: "Two short sequences have positive effects on the human p27 kip1 gene transcription"Gene. 228. 93-100 (1999)

[文献書誌] Yoshida, K,: "cDNA cloning and chromosomal mapping of mouse BH-protocadherin"DNA sequence. 10. 43-47 (1999)

[文献書誌] Yokoyama-Kobayashi, M.,: "Selection of cDNAs encoding putative type II membrane proteins on the cell surface from a human full-length cDNA bank"Gene. 228. 161-167 (1999)

[文献書誌] Ito, K.,: "cDNA cloning, characterization, and chromosome mapping of UBE2E3 (alias UbcH9), encoding and N-terminally extended human ubiquitin-conjugating enzyme"Cytogenet. Cell Genet. 84. 99-104 (1999)

[文献書誌] Komuro, A.,: "Npw38, a novel nuclear protein possessing a WW domain capable of activating basal transcription"Nucl. Acids Res.. 27. 1957-1965 (1999)

[文献書誌] Iwamuro, S.: "Multi-ubiquitination of a nascent membrane protein produced in a rabbit reticulocyte Iysate"J. Biochem.. 126. 48-53 (1999)

[文献書誌] Nunoi, H.,: "A heterozygous mutation of beta-actin associated with neutrophil dysfunction and recurrent infection"Proc. Natl. Acad. Sci. USA. 96. 8693-8698 (1999)

[文献書誌] Komuro, A.: "Association of two nuclear proteins, Npw38 and NpwBP, via the interaction between the WW domain and a novel proline-rich motif containing glycine and arginine"J. Biol. Chem.. 274. 36513-36519 (1999)

[文献書誌] Fujiwara K.: "Molecular cloning, structural characterization and chromosomal localization of human lipolytransferase gene"Eur J Biochem. 260. 761-767 (1999)

[文献書誌] Harada Y.: "Complete cDNA sequence and genomic organization of a human pancreas-specific gene homologous to Caenorhabiditis elegans sel-1"J. Hum Genet. 44. 330-336 (1999)

[文献書誌] Horie M.: "Cloning, expression and chromosomal napping of the human 14-3-3-gamma gene (YWHAG) to 7q11.23"Genomics. 60. 241-243 (1999)

[文献書誌] Hosokawa Y: "Human Aiolos, an Ikaros-related zinc finger DNA binding protein: cDNA cloning, tissue expression pattern, and chromosomal mapping"Genomics. 61. 326-329 (1999)

[文献書誌] Suzuki M.,: "Molecular cloning, expression, and mapping of a novel human cDNA, GRP17, highly homologous to human gadd45 and murine MyD118"J Hum Genet. 44. 300-303 (1999)

[文献書誌] Morohoshi F.: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1"Gene. 241. 287-295 (2000)

[文献書誌] Tabara, H.: "Pos-1 encodes a cytoplasmic zinc-finger protein essential for germline specification in C. elegans"Development. 126. 1-11 (1999)

[文献書誌] Fields, S.: "Functional Genomics"Proc. Natl. Acad. Sci. USA. 96. 8825-8826 (1999)

[文献書誌] Motii, M.,: "Identification of TGF-b-regulated genes in Caenorhabditis elegans by differential hybridization of arryaed cDNAs"Proc. Natl. Acad. Sci. USA. 96. 15020-15025 (1999)

[文献書誌] Cassata, C.,: "The Lim Homeobox Gene ceh-14 Confers Thermosensory Function to the AFD Neurons in Caenorhabditis elegans"Neuron. (in press).

[文献書誌] M. Maeda,: "Developmental changes in spatial expression of genes involved in myosin function of Dictyostelium"Dev. Biol.. (in press).

[文献書誌] Ito,. T.: "Toward a protein-protein interaction map of the budding yeast: A comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins"Proc. Natl. Acad. Sci. USA. 97. 1143-1147 (1999)

[文献書誌] Shiratori, A.,: "Systematic Identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and northern analysis"Yeast. 15. 219-253 (1999)

[文献書誌] Oda, T.,: "A novel TATA-binding protein-binding protein, ABT1, activates basal transcription and has yeast homolog that is essential for growth"Mol. Cell. Biol. 20. 1407-1418 (2000)

[文献書誌] Ogasawara, N.: "Systematic function analysis of Bacillus subtilis genes"Res Microbiol. (in press).

[文献書誌] Fukuchi, K.,: "The essential two-component regulatory system encoded by yycF and yycG genes modulates expression of the ftsAZ operon in Bacillus subtilis"Microbiology. (in press).

[文献書誌] Asai, K.,: "Regulation of the transport system for C(4)-dicarboxylic acids in Bacillus subtilis"Microbiology. 146. 263-271 (2000)

1999 年度実績報告書

小原雄治国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)