2000 年度実績報告書

ゲノムの機能解析

研究課題

研究課題/領域番号	08283105
研究機関	国立遺伝学研究所
研究代表者	小原雄治国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)
研究分担者	伊藤隆司金沢大学, がん研究所, 教授 (90201326) 菅野純夫東京大学, 医科学研究所, 助教授 (60162848) 大久保公策大阪大学, 細胞生体工学センター, 助教授 (40233069) 加藤菊也奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教授 (60194809) 小笠原直毅奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10110553)
キーワード	遺伝子発現 / EST / RNAi / 完全長cDNA / 発現パターン / 表現型 / 遺伝子破壊 / タンパク間相互作用
研究概要	大久保公策:ヒト2万遺伝子の基本解剖学発現パタンのBody Mapデータベースを構築し、トランスクリプトームの構造を明らかにした。加藤菊也:アダプター付加競合PCR法を開発し、マウス小脳皮質の生後発生過程の発現プロファイル解析を行った。菅野純夫:オリゴキャップ法を開発し、多数の完全長cDNAライブラリーを作製し遺伝子構造解析を行った。高橋永一:ヒト未知遺伝子162の機能解析を行った。相垣敏郎:ショウジョウバエGene Search系統を2,000作製し,機能獲得変異体の表現型情報を解析した。小原雄治:線虫C.elegansの半分強の7000cDNAの発現パターンを明らかにし、それをもとに母性発現500遺伝子の詳細なRNAi解析、クラスタ解析、調節領域の情報学的推定をおこなった。三谷昌平:線虫欠失変異体を従来法の約100倍の効率で分離するシステムを確立した。杉本亜砂子:soaking RNAi法を開発し、C.elegansの2500遺伝子の表現型解析を行った。前田ミネ子:細胞性粘菌のcDNAプロジェクトと発現パターンのダイナミクスの解析を進めた。伊藤隆司:酵母の全蛋白質を対象に2ハイブリッド法を用いた網羅的相互作用解析を行った。村上康文:出芽酵母第VI染色体遺伝子の発現パロフィールの解析を行った。小笠原直毅:枯草菌2674遺伝子の変異株作製・解析を、日欧共同プロジェクトとして完成した。

研究成果

(55件)

すべてその他

すべて文献書誌 (55件)

[文献書誌] Kawamoto S: "Body Map : A collection of 3′ESTs for analysis of human gene expression information."Genome Res.. 10(11). 1817-1827 (2000)
[文献書誌] Sese J: "Body Map incorporated PCR-based expression profiling data and a gene ranking system."Nucleic Acids Res.. 29(1). 156-158 (2001)
[文献書誌] Masuda N: "Clinical significance of micrometastases in axillary lymph nodes assessed by reverse transcription-polymerase chain reaction in breast cancer patients."Clin Cancer Res.. 6(11). 4176-4185 (2000)
[文献書誌] Takenaka M: "Gene expression profiles of the collecting duct in the mouse renal inner medulla."Kidney Int.. 57(1). 19-24 (2000)
[文献書誌] Hishiki T: "Body Map : a human and mouse gene expression database."Nucleic Acids Res.. 28(1). 136-138 (2000)
[文献書誌] Matoba,R.: "Gene expression in mouse cerebellum during its development."Gene. 241. 125-131 (2000)
[文献書誌] Matoba,R.: "Correlation between gene functions and developmental expression patterns in the mouse cerebellum."Eur.J.Neurosci.. 12. 1357-1371 (2000)
[文献書誌] Sakai,K.: "Microarray hybridization with fractionated cDNA : enhanced identification of differentially expressed genes."Anal Biochem.. 287. 32-37 (2000)
[文献書誌] Matoba,R.: "Gene expression profiling of mouse postnatal cerebellar development."Physiological Genomics. 4. 155-164 (2000)
[文献書誌] Ohmori,Y.: "Comparative PCR : A Simple and Sensitive Method to Quantify Low-Abundant mRNA Species."Genomics. 67. 140-145 (2000)
[文献書誌] Iizawa,M.: "Isolation and chromosomal assignment of a novel human gene, CORO1C, homologous to coronin-like actin-binding proteins."Cytogenet.Cell Genet.. 88. 221-224 (2000)
[文献書誌] Akashi,H.: "Isolation and characterization of a novel gene encoding a putative seven-span transmembrane protein, TM7SF3."Cytogenet.Cell Genet.. 88. 305-309 (2000)
[文献書誌] Akashi,H.: "Isolation and characterization of a human cDNA encoding a protein hohologous to the 7.2-kDa protein (subunit X) of bovine ubiquinol-cyctochrome C reductase."J.Hum.Genet.. 45. 43-46 (2000)
[文献書誌] Watanabe,M.: "Identification of canine a-lactalbumin."J Veterin.Med.Sci.. (in press).
[文献書誌] Watanabe,M.: "Cloning, expression analysis and chromosomal mapping of GTPBP2, a novel member of the G protein family."Gene. (in press).
[文献書誌] Yang,Z-Q.: "Identification of a novel gene, GASC1, within an amplicon at 9p23-24 frequently detected in esophageal cancer cell lines."Cancer Research. 60. 4735-4739 (2000)
[文献書誌] Yoshikawa,T.: "Isolation of novel mouse genes differentially expressed in brain using cDNA microarray."B.B.R.C.. 275. 532-537 (2000)
[文献書誌] Watanabe,J.: "FULL-malaria : a database for a full-length enriched cDNA library from human malaria parasite, Plasmodium falciparum."Nucl.Acid Res.. (in press).
[文献書誌] Watanabe,M.: "Inhibition of cell proliferation, suppression of tumourigenecity, and induction of differentiation of the canine mammary tumour cell line by sodium phenylacetate."Res.Yet.Sci.. (in press).
[文献書誌] Osada,N.: "Sequnce analysis, geneexpression, and chromosomal assignment of mouse Borg4 gene and its human orthologue."J.Hum.Genet.. 45. 374-377 (2000)
[文献書誌] Kawamoto T.: "Association between favorable neuroblastoma and high expression of the novel metalloproteinase gene, nbla3145/XCE, cloned by differential screening of the full-length-enriched oligo-capping neuroblastoma cDNA libraries."Med Pediatr Oncol.. 35. 628-631 (2000)
[文献書誌] Ohira M.: "Hunting the subset-specific genes of neuroblastoma : Expression profiling and differential screening of the full-length-enriched oligo-capping cDNA libraries."Med Pediatr Oncol.. 35. 547-549 (2000)
[文献書誌] Davuluri R.V.: "CART classification of human 5′ UTR sequences."Genome Res.. 10. 1807-1816 (2000)
[文献書誌] Campbell H.D.: "Human and mouse homologues of the drosophila melanogaster tweety (tty) gene : A novel gene family encoding predicted transmembrane proteins."Genomics.. 68. 89-92 (2000)
[文献書誌] Takasaki J: "The molecular characterization and tissue distribution of the human cysteinyl leukotriene CysLT (2) receptor."B.B.R.C.. 274. 316-322 (2000)
[文献書誌] Isaka S: "Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression."Genes Chromosomes and Cancer. 27. 345-352 (2000)
[文献書誌] Watanabe TK: "Characterization of newly developed SSLP markers for the rat.Mammalian"Genome. 11. 300-305 (2000)
[文献書誌] Hanaoka E: "Molecular cloning and expression analysis of the human DA41 gene and its mapping to chromosome 9q21.2-q21.3."J Hum Genet. 45. 188-191 (2000)
[文献書誌] Abe E: "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication."Gene. 225. 219-227 (2000)
[文献書誌] Yu Y: "Human papillomavirus type 33 DNA in breast cancer in chinase."Breast Cancer. 7(1). 33-36 (2000)
[文献書誌] Morohashi F: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1."Gene. 241. 287-295 (2000)
[文献書誌] Toba,G.: "Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster."Gene. 253. 179-187 (2000)
[文献書誌] Ejima,A.: "Phenotypic association of spontaneous ovulation and sexual receptivity in virgin females of Drosophila melanogaster mutants."Behavior Genetics. (in press). (2000)
[文献書誌] Cassata,C.: "The Lim Homeobox Gene ceh-14 Confers Thermosensory Function to the AFD Neurons in Caenorhabditis elegans."Neuron. 25. 587-597 (2000)
[文献書誌] Asahiha,M.: "A member of the ancient nuclear receptor class Ftz-F1 is required for embryogenesis, molting and reproduction in Caenorhabditis elegans."Genes to Cells. 5. 711-723 (2000)
[文献書誌] Nathalie Pujol: "The C.elegans unc-32 gene encodes alternative forms of the 100-kDa a subunit of Vacuolar-ATPases."J.Biol.Chem.. (in press). (2000)
[文献書誌] Maeda,I.: "Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi."Current Biology. 11No.3. 171-176 (2001)
[文献書誌] Reboul,J.: "Open-reading-frame sequence tags (OSTs) support the existence of at least 17, 300 genes in C.elegans."Nature Genetics. 27. 332-336 (2001)
[文献書誌] Gengyo-Ando,K.: "Characterization of mutations induced by ethylmethanesulfonate, UV and trimethylpsoralen in the nematode Caenorhabditis elegans."Biochem.Biophys.Res.Comm.. 269. 64-69 (2000)
[文献書誌] M.Maeda: "Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium."Dev.Biol.. 223. 114-119 (2000)
[文献書誌] T.Saito: "A second functional delta 5 desaturase in the cellular slime mold Dictyostelium discoideum."Eur.J.Biochem.. 267. 1813-1818 (2000)
[文献書誌] B.S.Mitra: "Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium."Gene. 251. 131-139 (2000)
[文献書誌] M.Tsujioka: "Spatial expression patterns of genes involved in cAMP-responses in Dictyostelium development."Develop.Growth Differ.. (in press).
[文献書誌] Ito,T.: "Toward a protein-protein interaction map of the budding yeast : a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins"Proc.Natl.Acad.Sci.USA. 97. 1143-1147 (2000)
[文献書誌] Kubota,H.: "GI domain-mediated association of the eukaryotic initiation factor 2alpha kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast"J.Biol.Chem.. 275. 20243-20246 (2000)
[文献書誌] Mizushima,K.: "A novel G-protein coupled receptor gene expressed in striatum"Genomics. 69. 314-321 (2000)
[文献書誌] Okamura,K.: "Comparative genome analysis of the mouse imprinted gene Impact and its nonimprinted human homolog IMPACT : toward the structural basis for species-specific imprinting"Genome Res.. 10. 1878-1889 (2000)
[文献書誌] Ito,T.: "A comprehensive two-hybrid analysis to explore the yeast protein interactome"Proc.Natl.Acad.Sci.USA. (in press). (2001)
[文献書誌] Oda,T.: "A novel TATA-binding protein-binding protein, ABT1, activates basal transcription and has yeast homolog that is essential for growth."Mol.Cell.Biol.. 20. 1407-1418 (2000)
[文献書誌] Ogasawara,N.: "Systematic function analysis of Bacillus subtilis genes."Res.Microbiol.. 151. 129-134 (2001)
[文献書誌] Asai,K.: "Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis."Microbiology. 146. 263-271 (2000)
[文献書誌] Fukuchi,K.: "The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis."Microbiology. 146. 1573-1583 (2000)
[文献書誌] Kodama,T.: "Synthesis and characterization of the spore proteins of Bacillus subtilis YdhD, YkuD, and YkvP, which carry a motif conserved among cell wall binding proteins."J Biochem(Tokyo). 128. 655-663 (2000)
[文献書誌] Takamatsu,H.: "The yabG gene of Bacillus subtilis encodes a sporulation specific protease which is involved in the processing of several spore coat proteins."FEMS Microbiol Lett. 192. 33-38 (2000)
[文献書誌] Takamatsu,H.: "The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition."J Bacteriol. 182. 1883-1888 (2000)

2000 年度 実績報告書

ゲノムの機能解析

研究代表者

小原 雄治 国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)

研究成果

[文献書誌] Kawamoto S: "Body Map : A collection of 3′ESTs for analysis of human gene expression information."Genome Res.. 10(11). 1817-1827 (2000)

[文献書誌] Sese J: "Body Map incorporated PCR-based expression profiling data and a gene ranking system."Nucleic Acids Res.. 29(1). 156-158 (2001)

[文献書誌] Masuda N: "Clinical significance of micrometastases in axillary lymph nodes assessed by reverse transcription-polymerase chain reaction in breast cancer patients."Clin Cancer Res.. 6(11). 4176-4185 (2000)

[文献書誌] Takenaka M: "Gene expression profiles of the collecting duct in the mouse renal inner medulla."Kidney Int.. 57(1). 19-24 (2000)

[文献書誌] Hishiki T: "Body Map : a human and mouse gene expression database."Nucleic Acids Res.. 28(1). 136-138 (2000)

[文献書誌] Matoba,R.: "Gene expression in mouse cerebellum during its development."Gene. 241. 125-131 (2000)

[文献書誌] Matoba,R.: "Correlation between gene functions and developmental expression patterns in the mouse cerebellum."Eur.J.Neurosci.. 12. 1357-1371 (2000)

[文献書誌] Sakai,K.: "Microarray hybridization with fractionated cDNA : enhanced identification of differentially expressed genes."Anal Biochem.. 287. 32-37 (2000)

[文献書誌] Matoba,R.: "Gene expression profiling of mouse postnatal cerebellar development."Physiological Genomics. 4. 155-164 (2000)

[文献書誌] Ohmori,Y.: "Comparative PCR : A Simple and Sensitive Method to Quantify Low-Abundant mRNA Species."Genomics. 67. 140-145 (2000)

[文献書誌] Iizawa,M.: "Isolation and chromosomal assignment of a novel human gene, CORO1C, homologous to coronin-like actin-binding proteins."Cytogenet.Cell Genet.. 88. 221-224 (2000)

[文献書誌] Akashi,H.: "Isolation and characterization of a novel gene encoding a putative seven-span transmembrane protein, TM7SF3."Cytogenet.Cell Genet.. 88. 305-309 (2000)

[文献書誌] Akashi,H.: "Isolation and characterization of a human cDNA encoding a protein hohologous to the 7.2-kDa protein (subunit X) of bovine ubiquinol-cyctochrome C reductase."J.Hum.Genet.. 45. 43-46 (2000)

[文献書誌] Watanabe,M.: "Identification of canine a-lactalbumin."J Veterin.Med.Sci.. (in press).

[文献書誌] Watanabe,M.: "Cloning, expression analysis and chromosomal mapping of GTPBP2, a novel member of the G protein family."Gene. (in press).

[文献書誌] Yang,Z-Q.: "Identification of a novel gene, GASC1, within an amplicon at 9p23-24 frequently detected in esophageal cancer cell lines."Cancer Research. 60. 4735-4739 (2000)

[文献書誌] Yoshikawa,T.: "Isolation of novel mouse genes differentially expressed in brain using cDNA microarray."B.B.R.C.. 275. 532-537 (2000)

[文献書誌] Watanabe,J.: "FULL-malaria : a database for a full-length enriched cDNA library from human malaria parasite, Plasmodium falciparum."Nucl.Acid Res.. (in press).

[文献書誌] Watanabe,M.: "Inhibition of cell proliferation, suppression of tumourigenecity, and induction of differentiation of the canine mammary tumour cell line by sodium phenylacetate."Res.Yet.Sci.. (in press).

[文献書誌] Osada,N.: "Sequnce analysis, geneexpression, and chromosomal assignment of mouse Borg4 gene and its human orthologue."J.Hum.Genet.. 45. 374-377 (2000)

[文献書誌] Kawamoto T.: "Association between favorable neuroblastoma and high expression of the novel metalloproteinase gene, nbla3145/XCE, cloned by differential screening of the full-length-enriched oligo-capping neuroblastoma cDNA libraries."Med Pediatr Oncol.. 35. 628-631 (2000)

[文献書誌] Ohira M.: "Hunting the subset-specific genes of neuroblastoma : Expression profiling and differential screening of the full-length-enriched oligo-capping cDNA libraries."Med Pediatr Oncol.. 35. 547-549 (2000)

[文献書誌] Davuluri R.V.: "CART classification of human 5′ UTR sequences."Genome Res.. 10. 1807-1816 (2000)

[文献書誌] Campbell H.D.: "Human and mouse homologues of the drosophila melanogaster tweety (tty) gene : A novel gene family encoding predicted transmembrane proteins."Genomics.. 68. 89-92 (2000)

[文献書誌] Takasaki J: "The molecular characterization and tissue distribution of the human cysteinyl leukotriene CysLT (2) receptor."B.B.R.C.. 274. 316-322 (2000)

[文献書誌] Isaka S: "Isolation and characterization of a novel TP53-inducible gene, TP53TG5, which suppresses growth and shows cell cycle-dependent transition of expression."Genes Chromosomes and Cancer. 27. 345-352 (2000)

[文献書誌] Watanabe TK: "Characterization of newly developed SSLP markers for the rat.Mammalian"Genome. 11. 300-305 (2000)

[文献書誌] Hanaoka E: "Molecular cloning and expression analysis of the human DA41 gene and its mapping to chromosome 9q21.2-q21.3."J Hum Genet. 45. 188-191 (2000)

[文献書誌] Abe E: "Structure, expression, and chromosomal localization of the human gene encoding a germinal center-associated nuclear protein (GANP) that associates with MCM3 involved in the initiation of DNA replication."Gene. 225. 219-227 (2000)

[文献書誌] Yu Y: "Human papillomavirus type 33 DNA in breast cancer in chinase."Breast Cancer. 7(1). 33-36 (2000)

[文献書誌] Morohashi F: "Structure and expression pattern of a human MTG8/ETO family gene, MTGR1."Gene. 241. 287-295 (2000)

[文献書誌] Toba,G.: "Disruption of the Microsomal glutathione S-transferase-like gene reduces life span of Drosophila melanogaster."Gene. 253. 179-187 (2000)

[文献書誌] Ejima,A.: "Phenotypic association of spontaneous ovulation and sexual receptivity in virgin females of Drosophila melanogaster mutants."Behavior Genetics. (in press). (2000)

[文献書誌] Cassata,C.: "The Lim Homeobox Gene ceh-14 Confers Thermosensory Function to the AFD Neurons in Caenorhabditis elegans."Neuron. 25. 587-597 (2000)

[文献書誌] Asahiha,M.: "A member of the ancient nuclear receptor class Ftz-F1 is required for embryogenesis, molting and reproduction in Caenorhabditis elegans."Genes to Cells. 5. 711-723 (2000)

[文献書誌] Nathalie Pujol: "The C.elegans unc-32 gene encodes alternative forms of the 100-kDa a subunit of Vacuolar-ATPases."J.Biol.Chem.. (in press). (2000)

[文献書誌] Maeda,I.: "Large-scale analysis of gene function in Caenorhabditis elegans by high-throughput RNAi."Current Biology. 11No.3. 171-176 (2001)

[文献書誌] Reboul,J.: "Open-reading-frame sequence tags (OSTs) support the existence of at least 17, 300 genes in C.elegans."Nature Genetics. 27. 332-336 (2001)

[文献書誌] Gengyo-Ando,K.: "Characterization of mutations induced by ethylmethanesulfonate, UV and trimethylpsoralen in the nematode Caenorhabditis elegans."Biochem.Biophys.Res.Comm.. 269. 64-69 (2000)

[文献書誌] M.Maeda: "Developmental changes in the spatial expression of genes involved in myosin function in Dictyostelium."Dev.Biol.. 223. 114-119 (2000)

[文献書誌] T.Saito: "A second functional delta 5 desaturase in the cellular slime mold Dictyostelium discoideum."Eur.J.Biochem.. 267. 1813-1818 (2000)

[文献書誌] B.S.Mitra: "Loss of a member of the aquaporin gene family, aqpA affects spore dormancy in Dictyostelium."Gene. 251. 131-139 (2000)

[文献書誌] M.Tsujioka: "Spatial expression patterns of genes involved in cAMP-responses in Dictyostelium development."Develop.Growth Differ.. (in press).

[文献書誌] Ito,T.: "Toward a protein-protein interaction map of the budding yeast : a comprehensive system to examine two-hybrid interactions in all possible combinations between the yeast proteins"Proc.Natl.Acad.Sci.USA. 97. 1143-1147 (2000)

[文献書誌] Kubota,H.: "GI domain-mediated association of the eukaryotic initiation factor 2alpha kinase GCN2 with its activator GCN1 is required for general amino acid control in budding yeast"J.Biol.Chem.. 275. 20243-20246 (2000)

[文献書誌] Mizushima,K.: "A novel G-protein coupled receptor gene expressed in striatum"Genomics. 69. 314-321 (2000)

[文献書誌] Okamura,K.: "Comparative genome analysis of the mouse imprinted gene Impact and its nonimprinted human homolog IMPACT : toward the structural basis for species-specific imprinting"Genome Res.. 10. 1878-1889 (2000)

[文献書誌] Ito,T.: "A comprehensive two-hybrid analysis to explore the yeast protein interactome"Proc.Natl.Acad.Sci.USA. (in press). (2001)

[文献書誌] Oda,T.: "A novel TATA-binding protein-binding protein, ABT1, activates basal transcription and has yeast homolog that is essential for growth."Mol.Cell.Biol.. 20. 1407-1418 (2000)

[文献書誌] Ogasawara,N.: "Systematic function analysis of Bacillus subtilis genes."Res.Microbiol.. 151. 129-134 (2001)

[文献書誌] Asai,K.: "Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis."Microbiology. 146. 263-271 (2000)

[文献書誌] Fukuchi,K.: "The essential two-component regulatory system encoded by yycF and yycG modulates expression of the ftsAZ operon in Bacillus subtilis."Microbiology. 146. 1573-1583 (2000)

[文献書誌] Kodama,T.: "Synthesis and characterization of the spore proteins of Bacillus subtilis YdhD, YkuD, and YkvP, which carry a motif conserved among cell wall binding proteins."J Biochem(Tokyo). 128. 655-663 (2000)

[文献書誌] Takamatsu,H.: "The yabG gene of Bacillus subtilis encodes a sporulation specific protease which is involved in the processing of several spore coat proteins."FEMS Microbiol Lett. 192. 33-38 (2000)

[文献書誌] Takamatsu,H.: "The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition."J Bacteriol. 182. 1883-1888 (2000)

2000 年度実績報告書

小原雄治国立遺伝学研究所, 生物遺伝資源情報総合センター, 教授 (70135292)