1998 年度実績報告書

ヒトゲノム構造解析

研究課題

研究課題/領域番号	08283108
研究機関	国立がんセンター
研究代表者	大木操国立がんセンター研究所, 放射線研究部, 部長 (00158792)
研究分担者	林健志九州大学, 遺伝子情報実験施設, 教授 (00019671) 阿部達生京都府立医科大学, 教授 (60079746) 猪子英俊東海大学, 医学部, 教授 (10101932) 清水信義慶応義塾大学, 医学部, 教授 (50162706) 榊佳之東京大学, 医科学研究所, 教授 (10112327)
キーワード	ヒト11q22-23領域 / S番染色体特異的BACクローン / ヒト21番染色体 / シークエンス解析 / HLAクラス1遺伝子群 / PLACE-SSCP / SKY法の改良 / Transcriptional sequencing
研究概要	ヒトllq22-23領域の一部、約15Mbの微細マップの作製とPACによるコンティグ化を行った。大量処理スクリーニング法によりSTS,ESTをプローブとして8番染色体特異的BACクローンを多数分離し、コンティグづくりの足場をつくった。ヒト21番染色体のシークエンス解析をq22.l領域を中心に行い全体で約9Mbの配列を決定し64個の遺伝子を推定した。8pll.2領域の一部約1.8Mbの塩基配列を決定し、3個の新規遺伝子を同定した。HLAクラスl遺伝子群の全領域1.8Mbの塩基配列を決定し60個の発現遺伝子と67個の偽遺伝子を見出した。ベーチェット病と尋常性乾癖症ついてマッピングを行いそれぞれの感受性遺伝子の領域を50kb以内に限定した。リンパ節欠損免疫不全突然変異マウス(ALY)の原因遺伝子がNIK遺伝子であることを明らかにした。2点連鎖解析により白内障等の座を特定化した。遺伝性神経疾患の病因遺伝子同定法として開発したDIRECT法を改良し増大CAGリピート検出の感度を上げた。福山型筋ジストロフィー原因遺伝子FCMDの遺伝子構造を決定した。膵臓がん等のLOH解折で共通欠失領域として同定した6q21,10q25,4q領域等のBAC等でのコンティグ化と候補遺伝子の検出を行った。l4q32領域のゲノム解折から、TCRα座との染色体転座によって活性化を受ける新規候補がん遺伝子TMLlを同定した。多数個体のDNAをプールしてPLACE-SSCP分析することにより、極めて能率よくSNPを検索する方法論を確立した。SKY法の改良を行い、これにより多数のMDS症例の複雑型染色体異常を解析し、欠失型異常の約半数が単純な欠失ではなく種々の染色体との不均漸型転座であることをあきらかにした。T7RNAポリメラーゼ及びribo-fluorescent3'-dNTPsを用いるTranscriptional scquencing技術を開発した。染色体特異的ゲノムクローンライブラリーの構築を継続し、9-12番染色体のライブラリーを作製した。

研究成果

(103件)

すべてその他

すべて文献書誌 (103件)

[文献書誌] Gamou,T.: "The partner gene of AMLI in t(16:21)myeloid malignencies is a novel member of the MTGS(ETO)family." Blood. 91. 4028-4037 (1998)
[文献書誌] Murakami,Y.: "Localization of tumor suppressor activity important in non small cell lung carcinoma on chromosome11q." Proc.Natl.Acad.Sci.,USA. 95. S153-S158 (1998)
[文献書誌] Morohoshi,F.: "Genomic structure of the human RBP56/HTAF_<11>68 and FUS/TLS genes." Gene. 221. 191-198 (1998)
[文献書誌] Eguchi-Ishimae,M.: "Fluorescence in situ hybridization analysis on 12;21 translocation in Japanese childhood acute lymphoblastie leukemia." Jpn.J.Cancer Res.89. 783-788 (1998)
[文献書誌] Shiraishi,M.: "The isolation of CpG islands from human chromosomal regions 11q13 and Xp22 by segregation of partlymelted molecules." Nuclec.Acids.Res.26. 5544-5550 (1998)
[文献書誌] Kitabayashi,I.: "The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR)family." Mol.Cell.Biol.18. 846-858 (1998)
[文献書誌] Sato,S.: "Splicing mutation of Presenilin-1 gene for early-onset familial Alzheimer's disease." Hum.Mutat.Supple.1. S91-S94 (1998)
[文献書誌] Tsukahara,F.: "Molecular chracterization of the mouse mtprd gene,a homologue of human TPRD: unique gene expression suggesting its critical role in the pathophysiology of Down Syndrome." J.Biochem.123. 1055-1063 (1998)
[文献書誌] Choi,D.K.: "Fluorescent differential display analysis of gene expression in apoptotic neuroblastoma cells." Gene. 223. 21-31 (1998)
[文献書誌] Koike,N.: "Identification of the mammalian homologues of the Drosophila timeless gene.Timeless 1." FEBS Letter. 441. 427-431 (1998)
[文献書誌] Hashida,H.: "Cloning and mapping of ZNF231,a novel brain-specific gene encoding neuronal double zine finger protein whose expression is enhanced in a neurodegenerative disorder,multiple system atrophy(MSA)." Genomics. 54. 50-58 (1998)
[文献書誌] Shiina,T.: "Nucleotide sequencing analysis of the 146kb segment around the 1kBL and MICA genes at the centromenric end of the HLA class I region." Genomics. 47. 372-382 (1998)
[文献書誌] Mizuki,N.: "Major histocompatibility complex class II alleles in an Uygur population in Northwest China." Tissue Antigens. 51. 287-292 (1998)
[文献書誌] Naruse,T.K.: "Extended HLA haplotyopes in Japanese homozygous typing cells." Tissue Antigens. 51. 305-308 (1998)
[文献書誌] Tamiya,G.: "Twenty-six new polymorphic microsatellites markers around the HAL-B.-C and -E loci in the human MHC class I region." Tissue Antigens. 51. 337-346 (1998)
[文献書誌] Katsuyama,Y.: "Genetic relationships among Japanese,Northern Han,Hui,Uygur,Kazakh,Greek,Saudi Arabian and Italian populations based on allelic frequencies at four VNTR (DIS80,D4S43,COL2A1,D17S5)and one STR (ACTBP2)Loci." Human Heredity. 48. 126-137 (1998)
[文献書誌] Taniguchi,Y.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. 51. 114-123 (1998)
[文献書誌] Ito,Y.: "Genomic structure of the spematid-specific Hsp70 homolog gene located in the class III region of the major histocompatibility complex of mouse and man." J.Biochem.124. 347-353 (1998)
[文献書誌] Sazazuki,T.: "Imporance of HLA-class I allele matching for clinical outcome after unrelated hematopoietic stem cell transplanation." N.Engl.J.Med.339. 1177-1185 (1998)
[文献書誌] Shiina,T.: "Physical mapping of 620 kb between the S and HLA-E genes in the midst of the human MHC class I region by construction of a BAC,PAC and cosmid contig." Immunogenetics. 48. 402-407 (1998)
[文献書誌] Kawakami,S.: "Tetex3,related to drosophila polycomblike,is expressed in male germ cells and mapped to the mouse-complex." Mamm.Genome.9. 874-880 (1998)
[文献書誌] Shigenari,A.: "Characterization of alkaline phosphatase genes expressed in seminoma by cDNA cloning." Cancer Res.58. 5079-5082 (1998)
[文献書誌] Yabuki,K.: "Triplet repeat polymorphism in the MICA gene in HLA-B27 positive and negative Caucasian patients with anlkylosing spondylitis." Hum.Immunol.60. 83-86 (1998)
[文献書誌] Ikeno,M.: "Construction of YAC based mammalian artificial chromosomes." Nature Biotech.16. 431-439 (1998)
[文献書誌] Masumoto,H.: "Assay of centromere function using a human artificial chromosome." Chromosoma. 107. 406-416 (1998)
[文献書誌] Igarashi,S.: "Suppression of aggregate formation and apoptosis by transglutaminase inhibitors in cells expressing truncated DRPLA protein with an expanded polyglutamine stretch." Nature Genet.18. 111-117 (1998)
[文献書誌] Saito,M.: "Refinement of the gene locus for autosomal recessive juvenile perkinsonism (AR-JP)on chromosome 6q25.2-27 and idenification markers exhibiting linkage disequilibrium." J.Hum.Genet.43. 22-31 (1998)
[文献書誌] Tanno,Y.: "Mitochondrial DNA polymorphisms in Japanese sporadic Alzheimer's disease." Neurobiol.Aging. 19. S47-S51 (1998)
[文献書誌] Ikeuchi,T.: "A novel long and unstable CAG/CTG trinucleotide repeat on chromosome 17q." Genomics. 49. 321-326 (1998)
[文献書誌] Takiyama,Y.: "Matemal anticipation in Machodo-Joseph disease (MJD):some matemal factors independent of the number of CAG repeat units may play a role in genetic anticipation i a Japanese MJD family." J.Nuerol.Sci.155. 141-145 (1998)
[文献書誌] Hermann.T.: "Molecular cloning,structural organization,sequence,chromosomal assignment, and expression of the mouse alpha-N-acetylgalactosaminidase gene." Gene. 211. 205-214 (1998)
[文献書誌] Onishi,Y.: "MERRF/MELAS overlap syndrome associated with 3243 tRNA^<Lew(UUR)> mutation of mitochondrial DNA." Neuropathology. 18. 321-327 (1998)
[文献書誌] Takano,H.: "Close associations between prevalence rates of dominantly inherited spinocerebellar ataxias with CAG-repeat expansions and frequencies of large normal CAG alleles in Japanese and Caucasian populations." Am.J.Hum.Genet.63. 1060-1066 (1998)
[文献書誌] Koide,T.: "Formation of granular cytoplasmic aggregates in cells expressing mutant Cu/Zn superoxide dismutase associated with familial amyotrophic lateral sclerosis." Neurosci.Lett.257. 29-32 (1998)
[文献書誌] Okuizumi,K.: "Genetic polymorphism of the tau gene and neurodegenerative diseases with tau pathology among Japanese." Ann.Neurol.44. 707 (1998)
[文献書誌] Sato,T: "Trangenic mice harboring a full-length human nutant DRPLA gene exhibit age-dependent intergenerational and somatic instabilities of CAG reptats comparable to those in DRPLA patients." Hum.Mol.Genet.8. 99-106 (1999)
[文献書誌] Takano,H.: "Mutational analysis and genotype-phenotype comelation of 29 unrelated Japanese patients with X-linked adenoleukodystrophy(ALD)" Arch.Neurol.(in press).
[文献書誌] Asakawa,S.: "High-fidelity digital hybridization screening." Genomics. 49. 209-217 (1998)
[文献書誌] Takahashi,H.: "Mouse myocilin (Myoc)gene expression in ocular tissues." Biochem.Biophys.Res.Commun.248. 104-109 (1998)
[文献書誌] Imamura,Y.: "Human retina-specific amine oxidase: genomic structure of the gene (AOC2)'altematively spliced variant,and mRNA expression in retina." Genomics. 51. 293-298 (1998)
[文献書誌] Footz,T.K.: "The gene for death agonist BID maps to the region of human 22q11.2 duplicated in cat eye syndrome chromosomes and to mouse chromosome 6." Genomics. 51. 472-475 (1998)
[文献書誌] Wang,J.: "Identification of two novel 5' noncoding exons in human MNB/DYRK gene and altematively spliced transcripts." Biochem.Biophys.Res.Commun.250. 704-710 (1998)
[文献書誌] Nagamine,K.: "Molecular cloning of a novel putative Ca^<2+> channel protein (TRPC7)higyly expressed in brain." Genomics. 54. 124-131 (1998)
[文献書誌] Sakamoto,O.: "Molecular analysis of new cases of Japanese patients of holocarboxylase synthetase deficiency." J.Inherit.Metab.Dis.21. 873-874 (1998)
[文献書誌] Kobayashi,K.: "An ancient retrotransposal insertion causes Fukuyama-type congenital muscular dystrophy." Nature. 394. 388-392 (1998)
[文献書誌] Kobayashi,K.: "Founder-haplotype analysis in Fukuyama-type congenital musclar dystrophy (FCMD)." Hum.Genel.103. 323-327 (1998)
[文献書誌] Takai,Y.: "Prenatal diagnosis of Fukuyama-type congenital muscular dystrophy by microsatellite analysis." Hum.Reprod.13. 320-323 (1998)
[文献書誌] Saito,K.: "Prenatal diagnosis in eight Fukuyama type congenital muscular dystrophy families by haplotype analysis using the new markers closest to the gene." Am.J.Med.Genet.26. 310-316 (1998)
[文献書誌] Voit,T.: "Merosin positive congenital muscular dystorophy with transient brain dysmyelination and mental retardation." Neuromuscular Disord. (in press).
[文献書誌] Toda,T.: "The Fukuyama-type congenital muscular dystrophy story." Neuromuscular Disord. (in press).
[文献書誌] Tsujita,T.: "Genomic discordance between monozygotic twins discordant for schizophrenia." Am.J.Psychiat.155. 422-424 (1998)
[文献書誌] Fujimoto,M.: "The gene for mesomelic dysplasia Kantaputra type is mapped to 2q24-q32." J.Hum.Genet.43. 32-36 (1998)
[文献書誌] Yun,K.: "Promoter-specific insulin-like growth factor2 gene imprinting in human fetal liver and hepatoblastoma." J.Pathol.185. 91-98 (1998)
[文献書誌] Maeda,T.: "Cloning and characterization of a novel human gene,TM4SF6,encoding a protein belonging to the transmembrane 4 superfamily,and mapped to Xq22." Genomics. 52. 240-242 (1998)
[文献書誌] Nakano,M.: "Identification,chracterization and mapping of the human ZIS(zinc-finger,splicing)gene." Gene. (in press).
[文献書誌] Ogaki,K.: "Mapping of a new target region of allelic loss to a 6-cM interval at 21q-21 in primary breast cancers." Genes.Chrom.Cancer.23. 244-247 (1998)
[文献書誌] Kurose,K.: "Frequent allelic loss at 7p14-15 associated with aggressive histologic types of breast cancer." Jpn.J.Cancer Res.89. 533-538 (1998)
[文献書誌] Minobe,K.: "Allelic loss on chromosome 9q is associated with lymph node metastasis of primary breast cancer." Jpn.J.Cancer.Res.89. 916-922 (1998)
[文献書誌] Kurose,K.: "Somatic mutations of the PTEN/MMACI gene in fifteen Japanese endometnal cancers: Evidence for inactivation of both alleles." Jpn.J.Cancer Res.89. 842-848 (1998)
[文献書誌] Iida.A.: "Mapping of a new target region of allelic loss to a 2-cM interval at 22q13.1 in primary breast cancer." Genes.Chrom.Cancer. 21. 108-112 (1998)
[文献書誌] Tsukamoto,K.: "Allelic loss on chromosome lp is associated with progression and lymph node metastasis of primary breast carcinoma." Cancer. 82. 317-322 (1998)
[文献書誌] Nakata,T.: "Identification of a new commonly deleted region within a 2-cM interval of 11p11 in breast cancers." Eur.J.Cancer. 34. 417-421 (1998)
[文献書誌] Bando,K.: "Frequent allelic loss at 6q26-27 in breast cancer of the solid-tubular histologic type." Breast Cancer. 5. 127-130 (1998)
[文献書誌] Keicho,N.: "Contribution of HLA genes to genetic predisposition in diffuse panbronchiolitis." Am.J.Respir.Crit.Care.Med.158. 846-850 (1998)
[文献書誌] Kamigaki,M.: "Familial hypercholesterolemia with cholesterry ester transfer protein deficinecy." Intem.Med.37. 523-527 (1998)
[文献書誌] Yamaki,E.: "Molecular genetic diagnosis of a family with hypercholesterolemia by a mismatched PCR-RFLP method for genotyping single base substitution of the LDL gene." Jpn.Heart.J.39. 681-686 (1998)
[文献書誌] Hirayama,T.: "Five familial hypereholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene." J.Hum.Genet.43. 250-254 (1998)
[文献書誌] Emi,M.: "Multiplex mutation screening of the BRCAI gene in 1000 Japanese breast cancers." Jpn.J.Cancer Res.89. 12-16 (1998)
[文献書誌] Yokota,T.: "Localization of a tumor suppressor gene associated with progression of human breast cancer within a 1-cM interval of 8p22-p23.1." Cancer. 83. 447-452 (1998)
[文献書誌] Kurose,K.: "Hamartomatous polyposis of the digestive tract with mutation of the PTEN gene." Am.J.Hum.Genet.64. 308-310 (1999)
[文献書誌] Nakata,T.: "Fusion of a novel gene,ELKS.to c-ret due to translocation t(10:12)(q11:p13)in a papillary thyroid carcinoma." Genes.Chrom.Cancer. 25(in press). (1999)
[文献書誌] Ohgaki,K.: "Localization of tumor suppressor gene associated with distant metastasis of urinary bladder cancer to I-Mb interval on 8p22." Genes.Chrom.Cancer. 25(in press). (1999)
[文献書誌] Fukino,K.: "Frequent ailelic loss at the TOC locus on 17q25.1 in primary breast cancers." Genes.Chrom.Cancer. 24. 345-350 (1999)
[文献書誌] Ueda,T.: "Idenification of a 1-cM region of common deletion of 13q14 associated with metastasis of human prostate cancer." Genes.Chrom.Cancer. 24. 183-190 (1999)
[文献書誌] Kitamura,Y.: "Ret/PTC3 is the most frequent form of gene rearrangement among papillary thyroid carcinomas in Japan." J.Hum.Genet.44. 96-102 (1999)
[文献書誌] Yokota,T.: "Frequent multiplication of chromosomal region 8q24.1 associated with aggressive histologic types of breast cancers." Cancer Lett.137. 1-7 (1999)
[文献書誌] Zhao,J.: "Expression of NPAT.a novel substrate of cyclin E-CDK2.promotes S-phase entry." Genes & Dev.12. 456-461 (1998)
[文献書誌] Sasaki,T.: "ATM mutations in patients with ataxia telangiectasia screened by a hierarchical strategy." Hum.Mutat.12. 186-195 (1998)
[文献書誌] Yamagishi,F.: "Molecular analysis of chromosome 14q32 abnormalities in esophageal and gastric carcinoma." Annats of Cancer Research and Therapy. 6. 85-88 (1998)
[文献書誌] Sakashita,A.: "Amplification of the TCLI flanking region at 14q32.1 with no TCLI gene transcription in a patient with peripheral T-cell lymphoma." Leukemia. 12. 970-971 (1998)
[文献書誌] Sugimoto,J.: "Isolation and mapping of a polymorphic CA repeat sequence at the human VRK1 locus." J.Hum.Genet.(in press).
[文献書誌] Bando,T.: "Loss of heterozygosity of chromosome 14q32 in colorectal carcinoma." Cancer Genet.Cytogenet.(in press).
[文献書誌] Sugimoto,J.: "Isolation and mapping of a putative b subunit of human ATP synthase (ATP-BL)from human leukocytes." DNA Res.(in press).
[文献書誌] Yamauchi,T.: "Structural organization of the human EIK1 gene and its processed pseudogene EIK2." DNA Res.(in press).
[文献書誌] Yatsuoka,T.: "Genomic analysis of the thymine-DNA glycosylase (TDG) gene on 12q22-q24.1 in human pancreatic ductal adencarcinoma ." Int.J.Pancreatol.(in press).
[文献書誌] Abe,T: "Identification of three commonly deleted regions on chromosome arm 6q in human pancreatic cancer." Genes.Chrom.Cancer.(in press).
[文献書誌] Furukawa,T.: "Genomic analysis of DUSP6,a dual specificity MAP kinase phosphatase,in pancreatic cancer." Cytogenet.Cell.Genet.82. 156-159 (1998)
[文献書誌] Yoshinaga,K.: "The PTEN,BAX and IGFIIR genes are mutated in endometrial atypical hyperplasia." Jpn.J.Cancer Res.89. 985-990 (1998)
[文献書誌] Yamakawa,H.: "Identification of a 100-kb region of common allelic loss on chromosome bands 10q25-q26 in human endometrial cancer." Genes.Chrom.Cancer.23. 74-77 (1998)
[文献書誌] Kimura,M.: "Identification of two common regions of allelic loss in chromosome arm 12q in human pancreatic cancer." Cancer Res.58. 2456-2460 (1998)
[文献書誌] Sasaki,N.: "Transcriptional sequencing: A method for DNA sequencing using RNA polymerase." Proc.Natl.Acad.Sci.,USA. 95. 3455-3460 (1998)
[文献書誌] Izawa,M.: "Recognition sites of 3'-OH group by T7 RNA polymerase and its application to transcriptional sequencing." J.Biol.Chem.273. 14242-14246 (1998)
[文献書誌] Sasaki,N.: "Identification of stable RNA hairpins causing band compression in transcriptional sequencing and their elimination by use of inosine triphosphate." Gene. 222. 17-24 (1998)
[文献書誌] Yoshikawa,H.: "A novel human gene located on Xp11.2-p11.4 which escapes X-inactivation detected and isoland using a two-dimensional DNA mapping method." Genomics. 49. 237-246 (1998)
[文献書誌] Abe,T.: "Infantile leukemia and soybeans-a hypothesis." Leukemia. (in press).
[文献書誌] Yashige,H.: "Micronuclei and nuclear abnomalities observed in erythroblasts in myelodysplastic syndromes and in de novo acute leukemia after treatment." Acta.Haematologica. (in press).
[文献書誌] Akiyama,Y.: "Anplification on double-minute chromosomes and partial-tandem duplication of the MLL gene in leukemic cells of a patient with acute myelogenous leukemia." Genes.Chrom.Cancer. 23. 267-272 (1998)
[文献書誌] Kishi,T.: "Hematopoietic cytokine-dependent differentiation to eosinophils and neutrophils in a newly established acute promyelocytic leukemia cell line with t(15;17)." Experimental Hematol.26. 135-142 (1998)
[文献書誌] Chikayama,S.: "Effects of daunorubicin on cell growth,cell cycle and induction of apoptosis in HL-60 cells." Hematologia. 29. 115-121 (1998)
[文献書誌] Shiina,T.: "Nucleotide sequencing determination of the 1.8 Mb entire HLA class 1 region." Monduzzi Editore.Bologna, 5 (1998)
[文献書誌] Shimizu,N.: "Human Genome Project: Current Status,Keio Strategy and Prospects." Springer/Verlag,Tokyo, 6 (1998)
[文献書誌] Hayashi,K.: "Single strand comformation polymorphism analysis,in Mutation Detection: A Practical Approach" Oxford University Press.Oxford, 18 (1998)
[文献書誌] Myers,R.M.: "Detection of Mutations,in Genome Analysis" Cold Spring Harbor Laboratory Press, 98 (1998)

1998 年度 実績報告書

ヒトゲノム構造解析

研究代表者

大木 操 国立がんセンター研究所, 放射線研究部, 部長 (00158792)

研究成果

[文献書誌] Gamou,T.: "The partner gene of AMLI in t(16:21)myeloid malignencies is a novel member of the MTGS(ETO)family." Blood. 91. 4028-4037 (1998)

[文献書誌] Murakami,Y.: "Localization of tumor suppressor activity important in non small cell lung carcinoma on chromosome11q." Proc.Natl.Acad.Sci.,USA. 95. S153-S158 (1998)

[文献書誌] Morohoshi,F.: "Genomic structure of the human RBP56/HTAF_<11>68 and FUS/TLS genes." Gene. 221. 191-198 (1998)

[文献書誌] Eguchi-Ishimae,M.: "Fluorescence in situ hybridization analysis on 12;21 translocation in Japanese childhood acute lymphoblastie leukemia." Jpn.J.Cancer Res.89. 783-788 (1998)

[文献書誌] Shiraishi,M.: "The isolation of CpG islands from human chromosomal regions 11q13 and Xp22 by segregation of partlymelted molecules." Nuclec.Acids.Res.26. 5544-5550 (1998)

[文献書誌] Kitabayashi,I.: "The AML1-MTG8 leukemic fusion protein forms a complex with a novel member of the MTG8(ETO/CDR)family." Mol.Cell.Biol.18. 846-858 (1998)

[文献書誌] Sato,S.: "Splicing mutation of Presenilin-1 gene for early-onset familial Alzheimer's disease." Hum.Mutat.Supple.1. S91-S94 (1998)

[文献書誌] Tsukahara,F.: "Molecular chracterization of the mouse mtprd gene,a homologue of human TPRD: unique gene expression suggesting its critical role in the pathophysiology of Down Syndrome." J.Biochem.123. 1055-1063 (1998)

[文献書誌] Choi,D.K.: "Fluorescent differential display analysis of gene expression in apoptotic neuroblastoma cells." Gene. 223. 21-31 (1998)

[文献書誌] Koike,N.: "Identification of the mammalian homologues of the Drosophila timeless gene.Timeless 1." FEBS Letter. 441. 427-431 (1998)

[文献書誌] Hashida,H.: "Cloning and mapping of ZNF231,a novel brain-specific gene encoding neuronal double zine finger protein whose expression is enhanced in a neurodegenerative disorder,multiple system atrophy(MSA)." Genomics. 54. 50-58 (1998)

[文献書誌] Shiina,T.: "Nucleotide sequencing analysis of the 146kb segment around the 1kBL and MICA genes at the centromenric end of the HLA class I region." Genomics. 47. 372-382 (1998)

[文献書誌] Mizuki,N.: "Major histocompatibility complex class II alleles in an Uygur population in Northwest China." Tissue Antigens. 51. 287-292 (1998)

[文献書誌] Naruse,T.K.: "Extended HLA haplotyopes in Japanese homozygous typing cells." Tissue Antigens. 51. 305-308 (1998)

[文献書誌] Tamiya,G.: "Twenty-six new polymorphic microsatellites markers around the HAL-B.-C and -E loci in the human MHC class I region." Tissue Antigens. 51. 337-346 (1998)

[文献書誌] Katsuyama,Y.: "Genetic relationships among Japanese,Northern Han,Hui,Uygur,Kazakh,Greek,Saudi Arabian and Italian populations based on allelic frequencies at four VNTR (DIS80,D4S43,COL2A1,D17S5)and one STR (ACTBP2)Loci." Human Heredity. 48. 126-137 (1998)

[文献書誌] Taniguchi,Y.: "Nucleotide sequence of the Ring3 gene in the class II region of the mouse MHC and its abundant expression in testicular germ cells." Genomics. 51. 114-123 (1998)

[文献書誌] Ito,Y.: "Genomic structure of the spematid-specific Hsp70 homolog gene located in the class III region of the major histocompatibility complex of mouse and man." J.Biochem.124. 347-353 (1998)

[文献書誌] Sazazuki,T.: "Imporance of HLA-class I allele matching for clinical outcome after unrelated hematopoietic stem cell transplanation." N.Engl.J.Med.339. 1177-1185 (1998)

[文献書誌] Shiina,T.: "Physical mapping of 620 kb between the S and HLA-E genes in the midst of the human MHC class I region by construction of a BAC,PAC and cosmid contig." Immunogenetics. 48. 402-407 (1998)

[文献書誌] Kawakami,S.: "Tetex3,related to drosophila polycomblike,is expressed in male germ cells and mapped to the mouse-complex." Mamm.Genome.9. 874-880 (1998)

[文献書誌] Shigenari,A.: "Characterization of alkaline phosphatase genes expressed in seminoma by cDNA cloning." Cancer Res.58. 5079-5082 (1998)

[文献書誌] Yabuki,K.: "Triplet repeat polymorphism in the MICA gene in HLA-B27 positive and negative Caucasian patients with anlkylosing spondylitis." Hum.Immunol.60. 83-86 (1998)

[文献書誌] Ikeno,M.: "Construction of YAC based mammalian artificial chromosomes." Nature Biotech.16. 431-439 (1998)

[文献書誌] Masumoto,H.: "Assay of centromere function using a human artificial chromosome." Chromosoma. 107. 406-416 (1998)

[文献書誌] Igarashi,S.: "Suppression of aggregate formation and apoptosis by transglutaminase inhibitors in cells expressing truncated DRPLA protein with an expanded polyglutamine stretch." Nature Genet.18. 111-117 (1998)

[文献書誌] Saito,M.: "Refinement of the gene locus for autosomal recessive juvenile perkinsonism (AR-JP)on chromosome 6q25.2-27 and idenification markers exhibiting linkage disequilibrium." J.Hum.Genet.43. 22-31 (1998)

[文献書誌] Tanno,Y.: "Mitochondrial DNA polymorphisms in Japanese sporadic Alzheimer's disease." Neurobiol.Aging. 19. S47-S51 (1998)

[文献書誌] Ikeuchi,T.: "A novel long and unstable CAG/CTG trinucleotide repeat on chromosome 17q." Genomics. 49. 321-326 (1998)

[文献書誌] Takiyama,Y.: "Matemal anticipation in Machodo-Joseph disease (MJD):some matemal factors independent of the number of CAG repeat units may play a role in genetic anticipation i a Japanese MJD family." J.Nuerol.Sci.155. 141-145 (1998)

[文献書誌] Hermann.T.: "Molecular cloning,structural organization,sequence,chromosomal assignment, and expression of the mouse alpha-N-acetylgalactosaminidase gene." Gene. 211. 205-214 (1998)

[文献書誌] Onishi,Y.: "MERRF/MELAS overlap syndrome associated with 3243 tRNA^<Lew(UUR)> mutation of mitochondrial DNA." Neuropathology. 18. 321-327 (1998)

[文献書誌] Takano,H.: "Close associations between prevalence rates of dominantly inherited spinocerebellar ataxias with CAG-repeat expansions and frequencies of large normal CAG alleles in Japanese and Caucasian populations." Am.J.Hum.Genet.63. 1060-1066 (1998)

[文献書誌] Koide,T.: "Formation of granular cytoplasmic aggregates in cells expressing mutant Cu/Zn superoxide dismutase associated with familial amyotrophic lateral sclerosis." Neurosci.Lett.257. 29-32 (1998)

[文献書誌] Okuizumi,K.: "Genetic polymorphism of the tau gene and neurodegenerative diseases with tau pathology among Japanese." Ann.Neurol.44. 707 (1998)

[文献書誌] Sato,T: "Trangenic mice harboring a full-length human nutant DRPLA gene exhibit age-dependent intergenerational and somatic instabilities of CAG reptats comparable to those in DRPLA patients." Hum.Mol.Genet.8. 99-106 (1999)

[文献書誌] Takano,H.: "Mutational analysis and genotype-phenotype comelation of 29 unrelated Japanese patients with X-linked adenoleukodystrophy(ALD)" Arch.Neurol.(in press).

[文献書誌] Asakawa,S.: "High-fidelity digital hybridization screening." Genomics. 49. 209-217 (1998)

[文献書誌] Takahashi,H.: "Mouse myocilin (Myoc)gene expression in ocular tissues." Biochem.Biophys.Res.Commun.248. 104-109 (1998)

[文献書誌] Imamura,Y.: "Human retina-specific amine oxidase: genomic structure of the gene (AOC2)'altematively spliced variant,and mRNA expression in retina." Genomics. 51. 293-298 (1998)

[文献書誌] Footz,T.K.: "The gene for death agonist BID maps to the region of human 22q11.2 duplicated in cat eye syndrome chromosomes and to mouse chromosome 6." Genomics. 51. 472-475 (1998)

[文献書誌] Wang,J.: "Identification of two novel 5' noncoding exons in human MNB/DYRK gene and altematively spliced transcripts." Biochem.Biophys.Res.Commun.250. 704-710 (1998)

[文献書誌] Nagamine,K.: "Molecular cloning of a novel putative Ca^<2+> channel protein (TRPC7)higyly expressed in brain." Genomics. 54. 124-131 (1998)

[文献書誌] Sakamoto,O.: "Molecular analysis of new cases of Japanese patients of holocarboxylase synthetase deficiency." J.Inherit.Metab.Dis.21. 873-874 (1998)

[文献書誌] Kobayashi,K.: "An ancient retrotransposal insertion causes Fukuyama-type congenital muscular dystrophy." Nature. 394. 388-392 (1998)

[文献書誌] Kobayashi,K.: "Founder-haplotype analysis in Fukuyama-type congenital musclar dystrophy (FCMD)." Hum.Genel.103. 323-327 (1998)

[文献書誌] Takai,Y.: "Prenatal diagnosis of Fukuyama-type congenital muscular dystrophy by microsatellite analysis." Hum.Reprod.13. 320-323 (1998)

[文献書誌] Saito,K.: "Prenatal diagnosis in eight Fukuyama type congenital muscular dystrophy families by haplotype analysis using the new markers closest to the gene." Am.J.Med.Genet.26. 310-316 (1998)

[文献書誌] Voit,T.: "Merosin positive congenital muscular dystorophy with transient brain dysmyelination and mental retardation." Neuromuscular Disord. (in press).

[文献書誌] Toda,T.: "The Fukuyama-type congenital muscular dystrophy story." Neuromuscular Disord. (in press).

[文献書誌] Tsujita,T.: "Genomic discordance between monozygotic twins discordant for schizophrenia." Am.J.Psychiat.155. 422-424 (1998)

[文献書誌] Fujimoto,M.: "The gene for mesomelic dysplasia Kantaputra type is mapped to 2q24-q32." J.Hum.Genet.43. 32-36 (1998)

[文献書誌] Yun,K.: "Promoter-specific insulin-like growth factor2 gene imprinting in human fetal liver and hepatoblastoma." J.Pathol.185. 91-98 (1998)

[文献書誌] Maeda,T.: "Cloning and characterization of a novel human gene,TM4SF6,encoding a protein belonging to the transmembrane 4 superfamily,and mapped to Xq22." Genomics. 52. 240-242 (1998)

[文献書誌] Nakano,M.: "Identification,chracterization and mapping of the human ZIS(zinc-finger,splicing)gene." Gene. (in press).

[文献書誌] Ogaki,K.: "Mapping of a new target region of allelic loss to a 6-cM interval at 21q-21 in primary breast cancers." Genes.Chrom.Cancer.23. 244-247 (1998)

[文献書誌] Kurose,K.: "Frequent allelic loss at 7p14-15 associated with aggressive histologic types of breast cancer." Jpn.J.Cancer Res.89. 533-538 (1998)

[文献書誌] Minobe,K.: "Allelic loss on chromosome 9q is associated with lymph node metastasis of primary breast cancer." Jpn.J.Cancer.Res.89. 916-922 (1998)

[文献書誌] Kurose,K.: "Somatic mutations of the PTEN/MMACI gene in fifteen Japanese endometnal cancers: Evidence for inactivation of both alleles." Jpn.J.Cancer Res.89. 842-848 (1998)

[文献書誌] Iida.A.: "Mapping of a new target region of allelic loss to a 2-cM interval at 22q13.1 in primary breast cancer." Genes.Chrom.Cancer. 21. 108-112 (1998)

[文献書誌] Tsukamoto,K.: "Allelic loss on chromosome lp is associated with progression and lymph node metastasis of primary breast carcinoma." Cancer. 82. 317-322 (1998)

[文献書誌] Nakata,T.: "Identification of a new commonly deleted region within a 2-cM interval of 11p11 in breast cancers." Eur.J.Cancer. 34. 417-421 (1998)

[文献書誌] Bando,K.: "Frequent allelic loss at 6q26-27 in breast cancer of the solid-tubular histologic type." Breast Cancer. 5. 127-130 (1998)

[文献書誌] Keicho,N.: "Contribution of HLA genes to genetic predisposition in diffuse panbronchiolitis." Am.J.Respir.Crit.Care.Med.158. 846-850 (1998)

[文献書誌] Kamigaki,M.: "Familial hypercholesterolemia with cholesterry ester transfer protein deficinecy." Intem.Med.37. 523-527 (1998)

[文献書誌] Yamaki,E.: "Molecular genetic diagnosis of a family with hypercholesterolemia by a mismatched PCR-RFLP method for genotyping single base substitution of the LDL gene." Jpn.Heart.J.39. 681-686 (1998)

[文献書誌] Hirayama,T.: "Five familial hypereholesterolemic kindreds in Japan with novel mutations of the LDL receptor gene." J.Hum.Genet.43. 250-254 (1998)

[文献書誌] Emi,M.: "Multiplex mutation screening of the BRCAI gene in 1000 Japanese breast cancers." Jpn.J.Cancer Res.89. 12-16 (1998)

[文献書誌] Yokota,T.: "Localization of a tumor suppressor gene associated with progression of human breast cancer within a 1-cM interval of 8p22-p23.1." Cancer. 83. 447-452 (1998)

[文献書誌] Kurose,K.: "Hamartomatous polyposis of the digestive tract with mutation of the PTEN gene." Am.J.Hum.Genet.64. 308-310 (1999)

[文献書誌] Nakata,T.: "Fusion of a novel gene,ELKS.to c-ret due to translocation t(10:12)(q11:p13)in a papillary thyroid carcinoma." Genes.Chrom.Cancer. 25(in press). (1999)

[文献書誌] Ohgaki,K.: "Localization of tumor suppressor gene associated with distant metastasis of urinary bladder cancer to I-Mb interval on 8p22." Genes.Chrom.Cancer. 25(in press). (1999)

[文献書誌] Fukino,K.: "Frequent ailelic loss at the TOC locus on 17q25.1 in primary breast cancers." Genes.Chrom.Cancer. 24. 345-350 (1999)

[文献書誌] Ueda,T.: "Idenification of a 1-cM region of common deletion of 13q14 associated with metastasis of human prostate cancer." Genes.Chrom.Cancer. 24. 183-190 (1999)

[文献書誌] Kitamura,Y.: "Ret/PTC3 is the most frequent form of gene rearrangement among papillary thyroid carcinomas in Japan." J.Hum.Genet.44. 96-102 (1999)

1998 年度実績報告書

大木操国立がんセンター研究所, 放射線研究部, 部長 (00158792)