1997 年度実績報告書

アゲハの幼虫食餌選択の機構

研究課題

研究課題/領域番号	08304048
研究機関	千葉大学
研究代表者	丸山工作千葉大学, 学長 (60012267)
研究分担者	蟻川謙太郎横浜市立大学, 理学部, 助教授 (20167232) 遠藤克彦山口大学, 理学部, 教授 (70089845) 御子柴克彦東京大学, 医科学研究所, 教授 (30051840) 神崎亮平筑波大学, 生物科学系, 講師 (40221907) 西田律夫京都大学, 農学部, 助教授 (30135545)
キーワード	産卵誘起物質 / アゲハチョウ / 昆虫の本能 / 感覚毛
研究概要	アゲハはミカン、カラタチなどミカン科植物の葉を食べて生育するが、メス成虫はこれらの食草を前肢感覚毛により数種類の化学物質で認識して産卵行動を起こす。今年度、サンショウの葉に含まれる5'-p-coumaroylquinic acid,epicatechin,hesperidin,hyperinが産卵刺激活性を示すことを実証した。チップレコーディング法を用いて、メス成虫前肢感覚毛の産卵刺激物質の電気的応答を検定した。その結果、食草植物抽出液に対しては小さい活動電位の反応がみられ、非食草植物に対しては大きな活動電位が生じ、明らかに応答の差がみられた。そこで前肢感覚毛は食草物質に対する特異的認識を示すことが判明した。個々の産卵刺激物質に対する応答はひきつづき研究中である。アゲハ前肢感覚毛に存在するミカン葉産卵刺激物質に特異的なレセプターを探索する試みがなされた。アゲハ500羽から前肢末端を切断、RNAを抽出、PCR法で増幅した。メス画分に多く、オス画分に少ない成分をdifferential display法で分画していくつかのcDNA断片を分析した。その1つはP450-チトクロムbオキシーダーゼと判明したが、レセプターcDNAはまだみつかっていない。アゲハの腹部末端にはオスにもメスにも光受容細胞が存在する。オスの光受容細胞はメスとの交尾に必須なことがわかった。メスの光受容細胞は交尾には必要ではないが、産卵には必要である。腹部末端から肛門突起を突き出させて葉をさぐりながら卵を産むさいに関与するものと思われる。ラットの嗅上皮細胞にgreen fluorescence protein(匂いレセプターの一種)をアデノウイルスベクター法によって過剰に発現させることに始めて成功した。それらオクチルアルデヒドに対して電気的に反応した。

研究成果
(40件)

すべてその他

すべて文献書誌 (40件)

[文献書誌] Aldrich,J.R., Leal,W.S., Nishida,R., et al..: "Semiochemistry of aposematic seed bugs(Heteroptera : Lygaeidae)." Entomol. Exp.84. 127-135 (1997)
[文献書誌] Amano,T., Nishida,R. & Kuwahara,Y.: "Ajugatakasins A and B, new diterpenoids from Ajuga decumbens, and feeding stimulative activity of related neoclerodane analogs toward the tumip saw fly." Biosci. Biotech. Biochem.61. 1518-1522 (1997)
[文献書誌] Nishida,R., Shelly,T.E. & Kaneshiro,K.Y.: "Acquisition of female-attracting fragrance from a Hawaiian lei flower, Fagraea berteriana, by males of the oriental fruit fly." J. Chem. Ecol.23. 2275-2285 (1997)
[文献書誌] Kanzaki,R.: "Co-ordination of wing motion and walking suggests common self-generated zigzag motor program in a male silkworm moth." J Comp Physiol A. 182. 267-276 (1998)
[文献書誌] Kuwana,Y., Nagasawa,S., Shimoyama,I. & Kanzaki,R.: "Synthesis of silkworm moth's pheromone-oriented behavior by a mobile robot with moth's antenna e as pheromone sensors." Biosensors and Bioelectronics.in press (1998)
[文献書誌] Del Rio,J.A., Heimrich,B., Borrell,V., et al.: "A role for Cajal-Retzius cells and reelin in the development of hippocampal connections." Nature.385. 70-74 (1997)
[文献書誌] Ohara-Imaizumi,M., Fukuda,M., et al.: "Distinct roles of C2A and C2B domains of synaptotagmin in the regulation of exocytosis in adrenal chromaffin cells." Proc. Natl. Acad. Sci. USA.94. 287-291 (1997)
[文献書誌] Morita,N., Nakahira,K., Baba,H., et al.: "Astrocytic Lineage Analysis by Detection of GFAP Promoter Activity in vitro." Developmental Neuroscience.19. 210-218 (1997)
[文献書誌] D'Arcangelo,G.K., Nakajima.T., et al: "Reelin is a secred glycoprotein recognized by the CR-50 monoclonal antibody." J. Neurosci.17. 23-31 (1997)
[文献書誌] Umemori,H., Inoue,T., Kume,S., et al.: "Activation of the G Protein Gq/11 Through Tyrosine Phosphorylation of the a Subunit." Science.276. 1878-1882 (1997)
[文献書誌] Nakajima,K., Mikoshiba,K., Miyata,T., et al.: "Distruption of hippocampal development in vivo by CR-50 mAb against Reelin." Proc. Natl. Acad. Sci.94. 8196-8201 (1997)
[文献書誌] Konishi,Y., Kobayashi,Y., Kishimoto,T., et al.: "Demonstration of an E-box and Transcriptional Regulation of the Mouse Type 1 Inositol 1, 4, 5-Trisphosphate Recepror Gene." J. Neurochem.69. 476-484 (1997)
[文献書誌] Nakata,K., Nagai,T. & Aruga,J., Mikoshiba,K.: "Xenopus Zic3, a primary regulator both in neural and neural crest development." Proc. Natl. Acad. Sci.94. 11980-11985 (1997)
[文献書誌] Morikawa,K., Ohbayashi,T., Nakagawa,M., et al.: "Transcription Initiation Sites and Promoter Structure of the Mouse Type 2 Inositol 1, 4, 5-Trisphospharate Receptor Gene." Gene.196. 181-185 (1997)
[文献書誌] Wada,E., Watase,K., Yamada,K., et al.: "Generation and Characterization of Mice Lacking Gastrin-Releasing Peptide Receptor. Biochem." Biochem. Biophys. Res. Comm.239. 28-33 (1997)
[文献書誌] Sheldon,M., Rice,S.D., D'Arcangelo,G., et al.: "Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice." Nature.389. 730-733 (1997)
[文献書誌] Zhao,H., Ivic,L., Otaki,JM., Hashimoto,M., et al.: "Functional expression of a mammalian odorant receptor." Science.279. 237-242 (1998)
[文献書誌] Maruyama,K.: "Connectin/titin, giant elastic protein of muscle." FASEB J.11. 341-345 (1997)
[文献書誌] Ohtsuka,H., Yajima,H., Maruyama,K. et al.: "The N-terminal Z repeat 5 of connectin/titin binds to the C-terminal region of α-actinin." Biochem. Biophys. Res. Commun.235. 1-3 (1997)
[文献書誌] Hisatsume,C., Umemori,H. Inoue,T., et. al.: "Phosphorylation-dependent Regulation of N-Methyl-D-asparate Receptors by calmodulin." J. Biol. Chem.272. 20805-20810 (1997)
[文献書誌] Satoh,A.K., Tokunaga,F. & Ozaki,K.: "Rab proteins of Drosophila meganogaster : Novel members of the rab-protein family." FEBS Lett.401. 65-69 (1997)
[文献書誌] Hisatomi,O., Ishino,T., Matsuda,S., et al.: "Functional expression and characterization of frog photoreceptor-specific calcium-binding proteins. Biochem. Biophys." Biochem. Biophys. Res. Comm.234. 173-177 (1997)
[文献書誌] Hisatomi,O., Imanishi,Y., Satoh,T. & Tokunaga,F.: "Arrestins expressed in killifish photoreceptor cells." FEBS Lett.411. 12-18 (1997)
[文献書誌] Hisatomi,O., Satoh,T. & Tokunaga,F.: "The primary structure and distribution of killifish visual pigments." Vision Res.37. 3089-3096 (1997)
[文献書誌] Mehrotra,B., Elliott,J.T., Chen,J., et al.: "Selective photoaffinity labeling of the inositol polyphosphate binding C2B domains of synaptotagmins." J. Biol. Chem.272. 4237-4244 (1997)
[文献書誌] Nagai,T., Aruga,J., Takeda,S. et al.: "The Expression of the Mouse Zic1, Zic2, and Zic3 Gene Suggests an Essential Role for Zic Genes in Body Pattern Formation." Develepmental Biology.182. 299-313 (1997)
[文献書誌] Miyata,T., Nakajima,K., Mikoshiba,K., Ogawa,M.: "Regulation of Purkinje Cell Alignment by Reelin as Revealed with CR-50 Antibody." J. Neurosci.17. 3599-3609 (1997)
[文献書誌] Fukuda,M., Kojima,T., Mikoshiba,K.: "Regulation by bivalent cations of phospholipid binding to the C2A domain of synaptotagmin III." Biochem. J.323. 421-425 (1997)
[文献書誌] Yoneshima,H. Miyawaki,A., Michikawa,T., et al.: "Ca^<2+> differetly regulates the ligand-affinity ststes of type 1 and type 3 inositol 1, 4, 5-trisphosphate receptors." Biochem. J.322. 591-596 (1997)
[文献書誌] Tanaka,D., Sakurama,T., Mitsumasu K., et al.: "Sep aration of bombyxin from a neuropeptide of Bombyx mori showing summer-morph-producing hormone(SMPH)activity in the Asian comma butterfly, Polygonia c-aureum L." J. Insect Physiol.43. 197-201 (1997)
[文献書誌] Starnecker,G., Koch,P.B., Matsumoto S., et al.: "Occurrence of four neuropeptides in the anterior central nervous system of the butterfly Precis coenia." Naturwiss.84. 152-154 (1997)
[文献書誌] Tsurumaki,J., Ishiguro,J., Yamanaka A. and Endo K.: "Effects of ph otoperiod and temperature on seasonal morph development and diapause egg oviposition in bivoltine race(Daizo)of the sulkmoth, Bombyx Mori L." J. Insect Physiol.44(in press). (1998)
[文献書誌] Hisatomi,O., Iwasa,T., et al.: "Characterization of lamprey rhodopsin by isolation and expression in a mammalian cells." Photochem. Photobiol.(in press). (1998)
[文献書誌] Liu,X., Wu,T., Stowe,S., Matsushita,A., et al.: "Defective phototransductive disk membrane morphogenesis in transgenic mice expressing opsin with a mutated N-terminal domain." J Cell Sci.110. 2589-2597 (1997)
[文献書誌] Matsushita,A. & Arikawa,K.: "Actin-based vesicular transport in the first 20 min after dusk is crucial for daily rhabdom synthesis in the compound eye of the grapsid crab, Hemigrapsus sanguineus." J Exp Biol.200. 2387-2392 (1997)
[文献書誌] Kinoshit,M., Sato,M. & Arikawa,K.: "Spectral receptors of nymphalid butterflies." Naturwiss.84. 199-201 (1997)
[文献書誌] Arikawa,K., Suyama,D. & Fujii,T.: "Hindsight by genitalia : Photo-guided copulation in butterflies." J Comp Physiol A.180. 295-299 (1997)
[文献書誌] Arikawa,K. & Stavenga,D.G.: "Random array of colour filters in the eyes of butterflies." J Exp Biol.200. 2501-2506 (1997)
[文献書誌] Arikawa,K., Mizuno,S., et al.: "An ultraviolet absorbing pigment causes a narrow-band violet receptor and a single-peaked green receptor in the eye of the butterfly Papilio." Vision Res,. (in press). (1998)
[文献書誌] Kitamoto,J., Sakamoto,K., Ozaki,K., et al.: "Identification of visual pigment opsins reveals the spectral receptor array in the retina of the butterfly, Papilio xuthus." J Exp Biol.(in press). (1998)

1997 年度 実績報告書

アゲハの幼虫食餌選択の機構

研究代表者

丸山 工作 千葉大学, 学長 (60012267)

研究成果

[文献書誌] Aldrich,J.R., Leal,W.S., Nishida,R., et al..: "Semiochemistry of aposematic seed bugs(Heteroptera : Lygaeidae)." Entomol. Exp.84. 127-135 (1997)

[文献書誌] Amano,T., Nishida,R. & Kuwahara,Y.: "Ajugatakasins A and B, new diterpenoids from Ajuga decumbens, and feeding stimulative activity of related neoclerodane analogs toward the tumip saw fly." Biosci. Biotech. Biochem.61. 1518-1522 (1997)

[文献書誌] Nishida,R., Shelly,T.E. & Kaneshiro,K.Y.: "Acquisition of female-attracting fragrance from a Hawaiian lei flower, Fagraea berteriana, by males of the oriental fruit fly." J. Chem. Ecol.23. 2275-2285 (1997)

[文献書誌] Kanzaki,R.: "Co-ordination of wing motion and walking suggests common self-generated zigzag motor program in a male silkworm moth." J Comp Physiol A. 182. 267-276 (1998)

[文献書誌] Kuwana,Y., Nagasawa,S., Shimoyama,I. & Kanzaki,R.: "Synthesis of silkworm moth's pheromone-oriented behavior by a mobile robot with moth's antenna e as pheromone sensors." Biosensors and Bioelectronics.in press (1998)

[文献書誌] Del Rio,J.A., Heimrich,B., Borrell,V., et al.: "A role for Cajal-Retzius cells and reelin in the development of hippocampal connections." Nature.385. 70-74 (1997)

[文献書誌] Ohara-Imaizumi,M., Fukuda,M., et al.: "Distinct roles of C2A and C2B domains of synaptotagmin in the regulation of exocytosis in adrenal chromaffin cells." Proc. Natl. Acad. Sci. USA.94. 287-291 (1997)

[文献書誌] Morita,N., Nakahira,K., Baba,H., et al.: "Astrocytic Lineage Analysis by Detection of GFAP Promoter Activity in vitro." Developmental Neuroscience.19. 210-218 (1997)

[文献書誌] D'Arcangelo,G.K., Nakajima.T., et al: "Reelin is a secred glycoprotein recognized by the CR-50 monoclonal antibody." J. Neurosci.17. 23-31 (1997)

[文献書誌] Umemori,H., Inoue,T., Kume,S., et al.: "Activation of the G Protein Gq/11 Through Tyrosine Phosphorylation of the a Subunit." Science.276. 1878-1882 (1997)

[文献書誌] Nakajima,K., Mikoshiba,K., Miyata,T., et al.: "Distruption of hippocampal development in vivo by CR-50 mAb against Reelin." Proc. Natl. Acad. Sci.94. 8196-8201 (1997)

[文献書誌] Konishi,Y., Kobayashi,Y., Kishimoto,T., et al.: "Demonstration of an E-box and Transcriptional Regulation of the Mouse Type 1 Inositol 1, 4, 5-Trisphosphate Recepror Gene." J. Neurochem.69. 476-484 (1997)

[文献書誌] Nakata,K., Nagai,T. & Aruga,J., Mikoshiba,K.: "Xenopus Zic3, a primary regulator both in neural and neural crest development." Proc. Natl. Acad. Sci.94. 11980-11985 (1997)

[文献書誌] Morikawa,K., Ohbayashi,T., Nakagawa,M., et al.: "Transcription Initiation Sites and Promoter Structure of the Mouse Type 2 Inositol 1, 4, 5-Trisphospharate Receptor Gene." Gene.196. 181-185 (1997)

[文献書誌] Wada,E., Watase,K., Yamada,K., et al.: "Generation and Characterization of Mice Lacking Gastrin-Releasing Peptide Receptor. Biochem." Biochem. Biophys. Res. Comm.239. 28-33 (1997)

[文献書誌] Sheldon,M., Rice,S.D., D'Arcangelo,G., et al.: "Scrambler and yotari disrupt the disabled gene and produce a reeler-like phenotype in mice." Nature.389. 730-733 (1997)

[文献書誌] Zhao,H., Ivic,L., Otaki,JM., Hashimoto,M., et al.: "Functional expression of a mammalian odorant receptor." Science.279. 237-242 (1998)

[文献書誌] Maruyama,K.: "Connectin/titin, giant elastic protein of muscle." FASEB J.11. 341-345 (1997)

[文献書誌] Ohtsuka,H., Yajima,H., Maruyama,K. et al.: "The N-terminal Z repeat 5 of connectin/titin binds to the C-terminal region of α-actinin." Biochem. Biophys. Res. Commun.235. 1-3 (1997)

[文献書誌] Hisatsume,C., Umemori,H. Inoue,T., et. al.: "Phosphorylation-dependent Regulation of N-Methyl-D-asparate Receptors by calmodulin." J. Biol. Chem.272. 20805-20810 (1997)

[文献書誌] Satoh,A.K., Tokunaga,F. & Ozaki,K.: "Rab proteins of Drosophila meganogaster : Novel members of the rab-protein family." FEBS Lett.401. 65-69 (1997)

[文献書誌] Hisatomi,O., Ishino,T., Matsuda,S., et al.: "Functional expression and characterization of frog photoreceptor-specific calcium-binding proteins. Biochem. Biophys." Biochem. Biophys. Res. Comm.234. 173-177 (1997)

[文献書誌] Hisatomi,O., Imanishi,Y., Satoh,T. & Tokunaga,F.: "Arrestins expressed in killifish photoreceptor cells." FEBS Lett.411. 12-18 (1997)

[文献書誌] Hisatomi,O., Satoh,T. & Tokunaga,F.: "The primary structure and distribution of killifish visual pigments." Vision Res.37. 3089-3096 (1997)

[文献書誌] Mehrotra,B., Elliott,J.T., Chen,J., et al.: "Selective photoaffinity labeling of the inositol polyphosphate binding C2B domains of synaptotagmins." J. Biol. Chem.272. 4237-4244 (1997)

[文献書誌] Nagai,T., Aruga,J., Takeda,S. et al.: "The Expression of the Mouse Zic1, Zic2, and Zic3 Gene Suggests an Essential Role for Zic Genes in Body Pattern Formation." Develepmental Biology.182. 299-313 (1997)

[文献書誌] Miyata,T., Nakajima,K., Mikoshiba,K., Ogawa,M.: "Regulation of Purkinje Cell Alignment by Reelin as Revealed with CR-50 Antibody." J. Neurosci.17. 3599-3609 (1997)

[文献書誌] Fukuda,M., Kojima,T., Mikoshiba,K.: "Regulation by bivalent cations of phospholipid binding to the C2A domain of synaptotagmin III." Biochem. J.323. 421-425 (1997)

[文献書誌] Yoneshima,H. Miyawaki,A., Michikawa,T., et al.: "Ca^<2+> differetly regulates the ligand-affinity ststes of type 1 and type 3 inositol 1, 4, 5-trisphosphate receptors." Biochem. J.322. 591-596 (1997)

[文献書誌] Tanaka,D., Sakurama,T., Mitsumasu K., et al.: "Sep aration of bombyxin from a neuropeptide of Bombyx mori showing summer-morph-producing hormone(SMPH)activity in the Asian comma butterfly, Polygonia c-aureum L." J. Insect Physiol.43. 197-201 (1997)

[文献書誌] Starnecker,G., Koch,P.B., Matsumoto S., et al.: "Occurrence of four neuropeptides in the anterior central nervous system of the butterfly Precis coenia." Naturwiss.84. 152-154 (1997)

[文献書誌] Tsurumaki,J., Ishiguro,J., Yamanaka A. and Endo K.: "Effects of ph otoperiod and temperature on seasonal morph development and diapause egg oviposition in bivoltine race(Daizo)of the sulkmoth, Bombyx Mori L." J. Insect Physiol.44(in press). (1998)

[文献書誌] Hisatomi,O., Iwasa,T., et al.: "Characterization of lamprey rhodopsin by isolation and expression in a mammalian cells." Photochem. Photobiol.(in press). (1998)

[文献書誌] Liu,X., Wu,T., Stowe,S., Matsushita,A., et al.: "Defective phototransductive disk membrane morphogenesis in transgenic mice expressing opsin with a mutated N-terminal domain." J Cell Sci.110. 2589-2597 (1997)

[文献書誌] Matsushita,A. & Arikawa,K.: "Actin-based vesicular transport in the first 20 min after dusk is crucial for daily rhabdom synthesis in the compound eye of the grapsid crab, Hemigrapsus sanguineus." J Exp Biol.200. 2387-2392 (1997)

[文献書誌] Kinoshit,M., Sato,M. & Arikawa,K.: "Spectral receptors of nymphalid butterflies." Naturwiss.84. 199-201 (1997)

[文献書誌] Arikawa,K., Suyama,D. & Fujii,T.: "Hindsight by genitalia : Photo-guided copulation in butterflies." J Comp Physiol A.180. 295-299 (1997)

[文献書誌] Arikawa,K. & Stavenga,D.G.: "Random array of colour filters in the eyes of butterflies." J Exp Biol.200. 2501-2506 (1997)

[文献書誌] Arikawa,K., Mizuno,S., et al.: "An ultraviolet absorbing pigment causes a narrow-band violet receptor and a single-peaked green receptor in the eye of the butterfly Papilio." Vision Res,. (in press). (1998)

[文献書誌] Kitamoto,J., Sakamoto,K., Ozaki,K., et al.: "Identification of visual pigment opsins reveals the spectral receptor array in the retina of the butterfly, Papilio xuthus." J Exp Biol.(in press). (1998)

1997 年度実績報告書

丸山工作千葉大学, 学長 (60012267)