1997 年度実績報告書

植物ウイルスの病原性の分子機構

研究課題

研究課題/領域番号	08306003
研究機関	京都大学
研究代表者	古澤巌京都大学, 農学研究科, 教授 (10026594)
研究分担者	渡辺雄一郎東京大学, 総合文化研究科・生命環境科学系, 助教授 (60183125) 石川雅之北海道大学, 農学部, 助教授 (70192482) 細川大二郎東京農工大学, 農学部, 教授 (50014957) 上田一郎北海道大学, 農学部, 教授 (10113523) 日比忠明東京大学, 農学部, 教授 (50261954)
キーワード	TMV / CMV / CaMV / PVX / RDV / RNA複製酵素 / 宿主因子 / 宿主特異性
研究概要	植物ウイルスのゲノム複製酵素についていろいろなウイルスを用いて総合的に研究を行った。Cauliflower mosaic virus(CaMV)の遺伝子産物のあるものはモノシストロニックmRNAから、また、あるものはポリシストロニックなmRNAから合成されていることが示唆され、ウイルスの複製の過程でCaMV特有の遺伝子発現機構を持っていることが明かとなった。Tobacco mosaic virus(TMV)RNAポリメラーゼの130K成分のmethyltransferase(M)ドメインおよび180K成分のRNA polymerase(P)ドメインにRNA結合活性が検出された。さらに、各ドメインに対する抗体を利用したアフィニティークロマトグラフィーによって、RNA合成活性を有する130K/180K複合体を単離することに成功した。Cucumber mosaic virus(CMV)の増殖が低レベルに抑制されるシロイヌナズナcuml及びcum2変異株の解析を行い、両変異のファインマッピングを行った。cuml変異が第4染色体中央部、cuml変異が第3染色体下部に位置することを明らかにし、現在遺伝子の同定を行っている。Potato X virus(PVX)を接種したタバコプロトプラストからサブゲノムRNAを合成する粗膜分画を得、この画分から界面活性剤によりサブゲノムRNAを合成するポリメラーゼを可溶化することに成功してた。Tobamovirusの複製酵素の979番目のアミノ酸の種類によって、トマトで知られるTm-1遺伝子による抵抗性に対する感受性が変化することが知られているが、イソロイシンにするとトマトで複製できなくなったウイルスがタバコでは複製できることが示され、宿主域決定因子との関連でこの領域が重要であることが分かった。

研究成果
(28件)

すべてその他

すべて文献書誌 (28件)

[文献書誌] Kappei Kobayashi: "Accumuiation Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)
[文献書誌] 海道正典: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" 日本植物病理学会報. 63(2). 95-98 (1997)
[文献書誌] Yukio Shirako: "Requirement for an Aromatic Amino Acid or Hisidine at the N Terminus of Sindbis Virus RNA Polymerase" Journal of Virology. 72(3). 2310-2315 (1998)
[文献書誌] Hiroshi Hamamoto: "Amino acid changes in the putative replicase of tomato mozaic tobamovirus that overvome resistance in Tm-1 tomato" Journal of General Virology. 78. 461-464 (1997)
[文献書誌] Hiroshi Hamamoto: "A Single Amino Acid Substitution in the Virus-Encoded Replicase of Tomato Mosaic Tobamovirus Alters Host Specificity" Molecular Plant-Microbe Interactions. 10(8). 1015-1018 (1997)
[文献書誌] Masayuki Ishikawa: "In VIvo DNA Expression of Functional Brome Mosaic Virus RNA Replicons in Saccharomyces cerevisiae" Journal of Virology. 71(10). 7781-7790 (1997)
[文献書誌] Masayuki Ishikawa: "Yeast mutation ins multiple complementation groups inhibit brome mosaic virus RNA replication and transcription and perturb regulated expression of the viral polymerase-like gene" Proc.Natl.Acad.Sci.USA. 94. 13810-13815 (1997)
[文献書誌] Shigenori Ueda: "Hypothesis on particle structure and assembly of rice dwarf phytoreovirus : interactions among multiple structural proteins" Journal of General Virology. 78. 3135-3140 (1997)
[文献書誌] Yoko Takahashi: "A cDNA Clone to Clover Yellow Vein Potyvirus Genome is Highly Infectious" Virus Genes. 14(3). 235-243 (1997)
[文献書誌] B.C.Lee: "Phylogenetic relationships between rice dwarf phytoreovirus isolates from five countries" European Journal of Plant Pathology. 103. 493-499 (1997)
[文献書誌] I.Uyeda: "The rice dwarf phytoreovirus structural protein P7 possesses non-specific nucleic acids binding activity in vitro" Molecular Plant Pathology. 1997. (1997)
[文献書誌] Motoyashi Yoshii: "Isolation of an Arabidopsis thaliana mutant in Which accumulation of cucumber mosaic virus coat protein is delayed" The Plant Journal. 13(2). 211-219 (1998)
[文献書誌] A.K.Kundu: "Nucleotide sequences of the coat protein genes of two Japanese Zucchini yellow mosaic virus isolates" Acta virologica. 41. 297-301 (1997)
[文献書誌] Yukio Shirako: "Non-AUG Translation Initiation in Plant RNA Virus : a Forty-Amino-Acid Extension Is Added to the N Terminus of the Soil-Borne Wheat Mosaic Virus Capsid Protein" Journal of Virology. 72(2). 1677-1682 (1998)
[文献書誌] Tomohisa Kuroda: "Formation of multimer of cucumber mozaic virus satelite RNA" Journal of General Virology. 78. 941-946 (1997)
[文献書誌] Talahide Sasaya: "Characterization and Nucleotide Sequence of the 3' Terminal Region of Clover Yellow Vein Isolated from Gentian (Gentiana spp.)" 日本植物病理学会報. 63(4). 328-333 (1997)
[文献書誌] Akira Karasawa: "A Possible Role of RNA 2 of Cucumber Mosaic Cucumovirus as a Determinant of Infection Phenotype on Cowpea" 日本植物病理学会報. 63(4). 289-297 (1997)
[文献書誌] H.Magome: "Molecular Variability of the Genomes of Capilloviruses from Apple,Japanene Pear,European Pear,and Citrus Trees" Molecular Plant Pathology. 87(4). 389-396 (1997)
[文献書誌] N.Yoshikawa: "Grapevine berry inner necrosis,a new trichovirus : comparative studies with several known trichoviruses" Archives of Virology. 142. 1351-1363 (1997)
[文献書誌] Hidetaka Terauchi: "Construction of an Infectios cDNA Clone of the Apple Stem Grooving Capillovirus (Isolate Li-23) Genome Containing a Cauliflower Mosaic Virus 35S RNA Promoter" 日本植物病理学会報. 63(6). 432-436 (1997)
[文献書誌] Hiroshi Magome: "Analysis of Double-stranded RNA in Tissues Infected with Apple Stem Grooving Capillovirus" 日本植物病理学会報. 63(6). 450-454 (1997)
[文献書誌] 佐藤正幸: "キュウリモザイクウイルス3分離株のダイコン品種における感染と増殖" 九州病害虫研究会報. 43. 39-41 (1997)
[文献書誌] Minoru Takeshita: "Complete Nucleotide Sequences of RNA3s of Cucumber Mosaic Virus KM and D8 Strains" J.Fag.Agr..,Kyusyu Univ.42(1・2). 27-32 (1997)
[文献書誌] Kiyoshi Ohshima: "Isolation of mutant of Arabidopsis thaliana carrying two simulataneous mutations affecting tobacco mosaic virus multiplication within a single cell" Virology. accepted
[文献書誌] Takuya Yamanaka: "Complete nucleotide sequence of the genomic RNA of tobacco mosaic virus strain Cg" Virus Genes. in press
[文献書誌] Shiro Kuninaga: "Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani" Curr Genet. 32. 237-243 (1997)
[文献書誌] T.Ohmori: "Characterzation of disease resistance gene-like sequences in near isogenic lines of tomato" Theor Apple Genet. 96. 000-000 (1998)
[文献書誌] 古澤巌: "「ウイルス学」(畑中正一編集)植物ラブドウイルスpp.468-470" 朝倉書店、東京, 646 (1997)

1997 年度 実績報告書

植物ウイルスの病原性の分子機構

研究代表者

古澤 巌 京都大学, 農学研究科, 教授 (10026594)

研究成果

[文献書誌] Kappei Kobayashi: "Accumuiation Kinetics of Viral Gene Products in Cauliflower Mosaic Virus-Infected Turnip Protoplasts" Microbiol.Immunol.42(1). 65-69 (1998)

[文献書誌] 海道正典: "Auto-cleavable Ribozyme Sequence Attached to Brome Mosaic Virus cDNA As Enhances Accumulation of Viral RNAs Transcribed In Vivo from the cDNAs" 日本植物病理学会報. 63(2). 95-98 (1997)

[文献書誌] Yukio Shirako: "Requirement for an Aromatic Amino Acid or Hisidine at the N Terminus of Sindbis Virus RNA Polymerase" Journal of Virology. 72(3). 2310-2315 (1998)

[文献書誌] Hiroshi Hamamoto: "Amino acid changes in the putative replicase of tomato mozaic tobamovirus that overvome resistance in Tm-1 tomato" Journal of General Virology. 78. 461-464 (1997)

[文献書誌] Hiroshi Hamamoto: "A Single Amino Acid Substitution in the Virus-Encoded Replicase of Tomato Mosaic Tobamovirus Alters Host Specificity" Molecular Plant-Microbe Interactions. 10(8). 1015-1018 (1997)

[文献書誌] Masayuki Ishikawa: "In VIvo DNA Expression of Functional Brome Mosaic Virus RNA Replicons in Saccharomyces cerevisiae" Journal of Virology. 71(10). 7781-7790 (1997)

[文献書誌] Masayuki Ishikawa: "Yeast mutation ins multiple complementation groups inhibit brome mosaic virus RNA replication and transcription and perturb regulated expression of the viral polymerase-like gene" Proc.Natl.Acad.Sci.USA. 94. 13810-13815 (1997)

[文献書誌] Shigenori Ueda: "Hypothesis on particle structure and assembly of rice dwarf phytoreovirus : interactions among multiple structural proteins" Journal of General Virology. 78. 3135-3140 (1997)

[文献書誌] Yoko Takahashi: "A cDNA Clone to Clover Yellow Vein Potyvirus Genome is Highly Infectious" Virus Genes. 14(3). 235-243 (1997)

[文献書誌] B.C.Lee: "Phylogenetic relationships between rice dwarf phytoreovirus isolates from five countries" European Journal of Plant Pathology. 103. 493-499 (1997)

[文献書誌] I.Uyeda: "The rice dwarf phytoreovirus structural protein P7 possesses non-specific nucleic acids binding activity in vitro" Molecular Plant Pathology. 1997. (1997)

[文献書誌] Motoyashi Yoshii: "Isolation of an Arabidopsis thaliana mutant in Which accumulation of cucumber mosaic virus coat protein is delayed" The Plant Journal. 13(2). 211-219 (1998)

[文献書誌] A.K.Kundu: "Nucleotide sequences of the coat protein genes of two Japanese Zucchini yellow mosaic virus isolates" Acta virologica. 41. 297-301 (1997)

[文献書誌] Yukio Shirako: "Non-AUG Translation Initiation in Plant RNA Virus : a Forty-Amino-Acid Extension Is Added to the N Terminus of the Soil-Borne Wheat Mosaic Virus Capsid Protein" Journal of Virology. 72(2). 1677-1682 (1998)

[文献書誌] Tomohisa Kuroda: "Formation of multimer of cucumber mozaic virus satelite RNA" Journal of General Virology. 78. 941-946 (1997)

[文献書誌] Talahide Sasaya: "Characterization and Nucleotide Sequence of the 3' Terminal Region of Clover Yellow Vein Isolated from Gentian (Gentiana spp.)" 日本植物病理学会報. 63(4). 328-333 (1997)

[文献書誌] Akira Karasawa: "A Possible Role of RNA 2 of Cucumber Mosaic Cucumovirus as a Determinant of Infection Phenotype on Cowpea" 日本植物病理学会報. 63(4). 289-297 (1997)

[文献書誌] H.Magome: "Molecular Variability of the Genomes of Capilloviruses from Apple,Japanene Pear,European Pear,and Citrus Trees" Molecular Plant Pathology. 87(4). 389-396 (1997)

[文献書誌] N.Yoshikawa: "Grapevine berry inner necrosis,a new trichovirus : comparative studies with several known trichoviruses" Archives of Virology. 142. 1351-1363 (1997)

[文献書誌] Hidetaka Terauchi: "Construction of an Infectios cDNA Clone of the Apple Stem Grooving Capillovirus (Isolate Li-23) Genome Containing a Cauliflower Mosaic Virus 35S RNA Promoter" 日本植物病理学会報. 63(6). 432-436 (1997)

[文献書誌] Hiroshi Magome: "Analysis of Double-stranded RNA in Tissues Infected with Apple Stem Grooving Capillovirus" 日本植物病理学会報. 63(6). 450-454 (1997)

[文献書誌] 佐藤正幸: "キュウリモザイクウイルス3分離株のダイコン品種における感染と増殖" 九州病害虫研究会報. 43. 39-41 (1997)

[文献書誌] Minoru Takeshita: "Complete Nucleotide Sequences of RNA3s of Cucumber Mosaic Virus KM and D8 Strains" J.Fag.Agr..,Kyusyu Univ.42(1・2). 27-32 (1997)

[文献書誌] Kiyoshi Ohshima: "Isolation of mutant of Arabidopsis thaliana carrying two simulataneous mutations affecting tobacco mosaic virus multiplication within a single cell" Virology. accepted

[文献書誌] Takuya Yamanaka: "Complete nucleotide sequence of the genomic RNA of tobacco mosaic virus strain Cg" Virus Genes. in press

[文献書誌] Shiro Kuninaga: "Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani" Curr Genet. 32. 237-243 (1997)

[文献書誌] T.Ohmori: "Characterzation of disease resistance gene-like sequences in near isogenic lines of tomato" Theor Apple Genet. 96. 000-000 (1998)

[文献書誌] 古澤 巌: "「ウイルス学」(畑中正一編集)植物ラブドウイルスpp.468-470" 朝倉書店、東京, 646 (1997)

1997 年度実績報告書

古澤巌京都大学, 農学研究科, 教授 (10026594)

[文献書誌] 古澤巌: "「ウイルス学」(畑中正一編集)植物ラブドウイルスpp.468-470" 朝倉書店、東京, 646 (1997)