1997 年度実績報告書

発生に関わる調節分子の全胚における発現・機能の発生工学的解析

研究課題

研究課題/領域番号	08307001
研究機関	島根医科大学
研究代表者	大谷浩島根医科大学, 医学部, 教授 (20160533)
研究分担者	山田源久留米大学, 先端癌治療研究センター, 助教授 (80174712) 南康博神戸大学, 医学部, 助教授 (70229772) 高橋直樹奈良先端科学技術大学院大学, 教授 (30179501) 阪上洋行東北大学, 医学部, 助手 (90261528) 饗場篤東京大学, 医科研, 助教授 (20271116)
キーワード	子宮外発生法 / 組織形成 / K-ras遺伝子 / CaMキナーゼ / 形態形成 / ホメオボックス遺伝子 / 受容体型チロシンキナーゼ / ノックアウトマウス
研究概要	1)マウス子宮外発生法を用い確立した系により、外脳症と無脳症の発生病理学的関係、LIFの大脳皮質形成における機能的意義ならびにACTHの胎生期副腎皮質の組織形成における機能的意義を解明した。また以下の研究を総括し子宮外発生法導入のための基礎的検討を行った。(大谷) 2)2種および3種のras群遺伝子を欠失したマウスを作成し、N-rasとK-ras、H-rasとK-rasがマウスの初期発生で共同して働くことが示唆された。また、rac1(-/-)マウスが胎生期致死であることから、Rac1が初期発生に必要であることを明らかにした。(饗場) 3)胎生期神経系により新規CaMキナーゼ探索過程で、Hela細胞プログラム細胞死関連プロテインキナーゼのラット相同分子の遺伝子クローニングに成功し、神経発達過程におけるその遺伝子発現局在を明らかにし、本分子が神経細胞死に関与する可能性を示唆した。(阪上) 4)マウスHoxの標的遺伝子として単離した遺伝子(mmab)の発現とHox遺伝子との関係を明らかにするため、レポーター遺伝子を結合したトランスジェニックマウスを作製した。また、遺伝子産物の機能を明らかにするため、抗体を調製し細胞内局在を調べた。(高橋) 5)ショウジョウバエの神経系に発現する新規受容体型チロシンキナーゼDnrkのマウス相同分子mRor1,mRor2の同定、発現解析を行った。さらに、mRor1,mRor2遺伝子のノックアウトマウス樹立及びexo utero解析で使用する組み換え蛋白質の作製を行った。(南) 6)homeobox遺伝子グ-スコイドが、従来の頭部-顔面形成過程に加え、骨盤、気管、外性器形成過程において発現しており、形態形成に機能している事を見いだした。この知見から、頭部-顔面とそれ以外の上記部位の形成過程で共通して形成プログラムがある可能性を示唆した。(山田)

研究成果
(30件)

すべてその他

すべて文献書誌 (30件)

[文献書誌] Hashimoto,R.: "Selective translocation of different markers in the ante-and retrograde pathways between the Golgi apparatus and the rough endoplasmic reticulum in a hybridoma cell line." Ann Anat.179. 105-116 (1997)
[文献書誌] Sekimoto,H.: "Developmental analysis of chlorambucil-induced occipital bleds in mice." Cong.Anom.37. 31-45 (1997)
[文献書誌] Matsuda,S.: "Apoptosis in the development of the temporomandibular joint." Anat.Embryol.196. 383-391 (1997)
[文献書誌] Omori,H.: "Deuterium oxide(heavy water)accelerates actin assembly in vitro and changes microfilament distribution in cultured cells." Eur.J.Cell Biol.74. 273-280 (1997)
[文献書誌] 大谷浩: "初期発生:卵割から胚葉形成まで." 生態の科学. 49. 54-61 (1998)
[文献書誌] Zhang,H.: "Induction of ectopic corticotrophic tumor in mouse embryos by exo utero cell transplantation and its effects on the fetal adrenal gland." Endocrinology. (in press).
[文献書誌] Koera.K.: "K-ras in essential for the development of the mouse embryo." Oncogene. 15. 1151-1159 (1997)
[文献書誌] Sakagami,H.: "Molecular cloning and developmental expression of a rat homologue of death-associated protein kinase in the nervous system." Molecular Brain Research. 52. 249-256 (1997)
[文献書誌] Sakagami,H.: "Localization of the mRNAs for two isoforms of Ca2+/calmodulin-dependent protein kinase kinsaes in the adult rat brain." Molecular Brain Research. (in press).
[文献書誌] Ono,F.: "A novel class II phosphoinositide 3-kinase predominantly expressed in the liver and its enhanced expression during liver regeneration." J.Biological Chemistry. (in press).
[文献書誌] 阪上洋行: "Ca2+/カルモデュリン依存性プロテインキナーゼIVに関する形態学的研究" Acta Anatomica Nipponica. (in press).
[文献書誌] Ito,T.: "A serine/threonine protein kinase gene isolated by an in vivo binding procedure using the Arabidopsis floral homeotic gene product,AGAMOUS." Plant Cell Physiol.38. 248-258 (1997)
[文献書誌] Ohuchi,H.: "Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4." Development. 125. 51-60 (1998)
[文献書誌] Ueno,N.: "Identification of a novel Drosophila protein kinase highly homologous to protein kinase N(PKN)." Biochem.Biophys.Res.Commun.232. 126-129 (1997)
[文献書誌] Oishi,I.: "A novel Drosophila receptor tyrosine kinase expressed specifically in the nervous system." J.Biol.Chem.272. 11916-11923 (1997)
[文献書誌] Chen,M.: "The amino terminus of Jak3 is necessary and sufficient for binding to the common g chain and confers the ability to transmit interleukin 2-mediated signals." Proc.Natil.Acad.Sci.USA. 94. 6910-6915 (1997)
[文献書誌] Oishi,I.: "A novel Drosophila nuclear protein serine/threonine kinase expressed in the germline during its establishment." Mech.Dev.(in press)
[文献書誌] G.Yamada: "Trace element composition and histological analysis of rat bones from the space shuttle." Life Sciences. 60. 635-642 (1997)
[文献書誌] G.Yamada: "Nasal and pharyngeal abnormalities caused by the mouse Goosecoid gene mutation." BBRC. 233. 161-165 (1997)
[文献書誌] C.Zhu: "Correlation between loss of middle ear bones and altered goosecoid gene expression in the branchial region following retinoic acid treatment of mouse embryos in vivo." BBRS. 235. 748-753 (1997)
[文献書誌] G.Yamada: "An efficient liposome-mediated gene trasnsfer into the branchial arch,neural tube and the heart of chick embryos ; A strategy to elucidate the organogenesis." Cell.Mol.Biol.43. 1165-1169 (1997)
[文献書誌] G.Yamada: "Microphthalmia(mi)Mice Display an Aberrant Bone Trace Element Composition." Biol.Trace.Ele.Res.62(in press). (1998)
[文献書誌] G.Yamada: "Aberrant regulation of bone trace elements in Motheaten(me)mice and Osteopetrosis(op)mice." Cell.Mol.Biol.44(in press). (1998)
[文献書誌] C.Zhu: "Malformation of trachea,pelvic region and external genitalia in goosecoid mutant mice" Dev.Dyn.(in press).
[文献書誌] Kano,M.: "Presistent Multiple Climbing Fiber Innervation of Cerebellar Purkinje Cells in Mice Lacking mGluR1." Neuron. 18. 71-79 (1997)
[文献書誌] 森山賢治: "ノックアウトマウスデータブック(Molecular Medicine臨時増刊)" 中山書店, 564 (1997)
[文献書誌] Sakagami,H.: "1st Hirosaki International Forum of Medical Science : Dynamic Cells : Cell Biology of the 21st Century held in Hirosaki on 30 October 1997." Elsevier Science(in press),
[文献書誌] 大崎加奈枝: "ノックアウトマウスデータブック(Molecular Medicine臨時増刊)" 中山書店, 564 (1997)
[文献書誌] 高橋直樹: "図解生物学講座5、分子生物学" 朝倉書店, (1998)
[文献書誌] Oishi,I.: "Kinases andphosphatases in lymphocyte and neuronal signaling" Springer, 368 (1997)

1997 年度 実績報告書

発生に関わる調節分子の全胚における発現・機能の発生工学的解析

研究代表者

大谷 浩 島根医科大学, 医学部, 教授 (20160533)

研究成果

[文献書誌] Hashimoto,R.: "Selective translocation of different markers in the ante-and retrograde pathways between the Golgi apparatus and the rough endoplasmic reticulum in a hybridoma cell line." Ann Anat.179. 105-116 (1997)

[文献書誌] Sekimoto,H.: "Developmental analysis of chlorambucil-induced occipital bleds in mice." Cong.Anom.37. 31-45 (1997)

[文献書誌] Matsuda,S.: "Apoptosis in the development of the temporomandibular joint." Anat.Embryol.196. 383-391 (1997)

[文献書誌] Omori,H.: "Deuterium oxide(heavy water)accelerates actin assembly in vitro and changes microfilament distribution in cultured cells." Eur.J.Cell Biol.74. 273-280 (1997)

[文献書誌] 大谷 浩: "初期発生:卵割から胚葉形成まで." 生態の科学. 49. 54-61 (1998)

[文献書誌] Zhang,H.: "Induction of ectopic corticotrophic tumor in mouse embryos by exo utero cell transplantation and its effects on the fetal adrenal gland." Endocrinology. (in press).

[文献書誌] Koera.K.: "K-ras in essential for the development of the mouse embryo." Oncogene. 15. 1151-1159 (1997)

[文献書誌] Sakagami,H.: "Molecular cloning and developmental expression of a rat homologue of death-associated protein kinase in the nervous system." Molecular Brain Research. 52. 249-256 (1997)

[文献書誌] Sakagami,H.: "Localization of the mRNAs for two isoforms of Ca2+/calmodulin-dependent protein kinase kinsaes in the adult rat brain." Molecular Brain Research. (in press).

[文献書誌] Ono,F.: "A novel class II phosphoinositide 3-kinase predominantly expressed in the liver and its enhanced expression during liver regeneration." J.Biological Chemistry. (in press).

[文献書誌] 阪上洋行: "Ca2+/カルモデュリン依存性プロテインキナーゼIVに関する形態学的研究" Acta Anatomica Nipponica. (in press).

[文献書誌] Ito,T.: "A serine/threonine protein kinase gene isolated by an in vivo binding procedure using the Arabidopsis floral homeotic gene product,AGAMOUS." Plant Cell Physiol.38. 248-258 (1997)

[文献書誌] Ohuchi,H.: "Correlation of wing-leg identity in ectopic FGF-induced chimeric limbs with the differential expression of chick Tbx5 and Tbx4." Development. 125. 51-60 (1998)

[文献書誌] Ueno,N.: "Identification of a novel Drosophila protein kinase highly homologous to protein kinase N(PKN)." Biochem.Biophys.Res.Commun.232. 126-129 (1997)

[文献書誌] Oishi,I.: "A novel Drosophila receptor tyrosine kinase expressed specifically in the nervous system." J.Biol.Chem.272. 11916-11923 (1997)

[文献書誌] Chen,M.: "The amino terminus of Jak3 is necessary and sufficient for binding to the common g chain and confers the ability to transmit interleukin 2-mediated signals." Proc.Natil.Acad.Sci.USA. 94. 6910-6915 (1997)

[文献書誌] Oishi,I.: "A novel Drosophila nuclear protein serine/threonine kinase expressed in the germline during its establishment." Mech.Dev.(in press)

[文献書誌] G.Yamada: "Trace element composition and histological analysis of rat bones from the space shuttle." Life Sciences. 60. 635-642 (1997)

[文献書誌] G.Yamada: "Nasal and pharyngeal abnormalities caused by the mouse Goosecoid gene mutation." BBRC. 233. 161-165 (1997)

[文献書誌] C.Zhu: "Correlation between loss of middle ear bones and altered goosecoid gene expression in the branchial region following retinoic acid treatment of mouse embryos in vivo." BBRS. 235. 748-753 (1997)

[文献書誌] G.Yamada: "An efficient liposome-mediated gene trasnsfer into the branchial arch,neural tube and the heart of chick embryos ; A strategy to elucidate the organogenesis." Cell.Mol.Biol.43. 1165-1169 (1997)

[文献書誌] G.Yamada: "Microphthalmia(mi)Mice Display an Aberrant Bone Trace Element Composition." Biol.Trace.Ele.Res.62(in press). (1998)

[文献書誌] G.Yamada: "Aberrant regulation of bone trace elements in Motheaten(me)mice and Osteopetrosis(op)mice." Cell.Mol.Biol.44(in press). (1998)

[文献書誌] C.Zhu: "Malformation of trachea,pelvic region and external genitalia in goosecoid mutant mice" Dev.Dyn.(in press).

[文献書誌] Kano,M.: "Presistent Multiple Climbing Fiber Innervation of Cerebellar Purkinje Cells in Mice Lacking mGluR1." Neuron. 18. 71-79 (1997)

[文献書誌] 森山賢治: "ノックアウトマウスデータブック(Molecular Medicine臨時増刊)" 中山書店, 564 (1997)

[文献書誌] Sakagami,H.: "1st Hirosaki International Forum of Medical Science : Dynamic Cells : Cell Biology of the 21st Century held in Hirosaki on 30 October 1997." Elsevier Science(in press),

[文献書誌] 大崎加奈枝: "ノックアウトマウスデータブック(Molecular Medicine臨時増刊)" 中山書店, 564 (1997)

[文献書誌] 高橋直樹: "図解生物学講座5、分子生物学" 朝倉書店, (1998)

[文献書誌] Oishi,I.: "Kinases andphosphatases in lymphocyte and neuronal signaling" Springer, 368 (1997)

1997 年度実績報告書

大谷浩島根医科大学, 医学部, 教授 (20160533)

[文献書誌] 大谷浩: "初期発生:卵割から胚葉形成まで." 生態の科学. 49. 54-61 (1998)