1997 年度実績報告書

蛋白質集合体の対称性の起源と系譜-機能的要請か、系統性か

研究課題

研究課題/領域番号	08309010
研究機関	山口大学
研究代表者	三室守山口大学, 理学部, 教授 (40142004)
研究分担者	嶋田敬三東京都立大学, 大学院・理学研究科, 助教授 (80112473) 植田邦彦金沢大学, 理学部, 教授 (60184925) 郷通子名古屋大学, 大学院・理学研究科, 教授 (70037290) 藤吉好則京都大学, 大学院・理学研究科, 教授 (80142298)
キーワード	蛋白質集合体の対称性 / 系統・進化 / 蛋白質立体構造 / モデュール構造 / 構造-機能相関
研究概要	光合成、視覚などのエネルギー変換、情報変換過程で機能する蛋白質集合体でしばしば観察される蛋白質分子の対称構造の意味を、(1)機能発現のための必然性、(2)起源が異なる蛋白質の系統性、のふたつの観点から考察した。具体的な解析のポイントは、(1)補欠分子間の相互作用、(2)ドメイン、さらにモデュール構造であった。解析の対象として、光合成系反応中心(C_2対称)、アンテナ複合体(C_3対称)、ロドプシンファミリー(C_3対称、対称性は不変)、対称性と同じ意味で重要な「対称性の破れと機能発現」の観点から、ATPase(偽C_3対称、対称性は不変)を考察した。光合成アンテナ系の場合、対称構造を取ることは最も容易に光を集める高率を高めることが可能な方法である。しかしこれを機能発現の「必然性」として理解できる例は多くはない。例えば、フィコビリン蛋白質はグロビン蛋白質のN末端側にヘリックス構造を加えて、グロビン蛋白質とは異なる対称性を確保し、同時に機能発現のために色素間での相互作用を可能にしたケースである。この場合、基準振動解析から構造的な必然性を証明することができた。 ATPaseは,機能の発現のためにその対称性を破ることが必然であることが最近明らかになった3次元構造からも理解された。班会議での結論は、この課題は今後,蛋白質の3次元構造データが集積する中で、機能と密度に結び付いた課題であり、長期的な展望に立って継続する必要があることが確認された。

研究成果

(28件)

すべてその他

すべて文献書誌 (28件)

[文献書誌] Tomioka,H.: "Voltage-dependent absorbance change of carotenoids in halophilic archaebacteria." Biochemica et Biophysica Acta. 1284. 79-85 (1996)
[文献書誌] Tomioka,H.: "Real-Time image processing using polarization discrimination of bacteriorhodopsin." Synthetic metals. 81. 147-149 (1996)
[文献書誌] Tomioka,H.: "Detection and expression of a gene encoding a novel bacteriorhodopsin from an extreme halophile strain HT(JCM9743)which possesses on bacteriorhopsin activity." Extremophiles. 2. 33-39 (1998)
[文献書誌] Go,M: "Cloning and characterization of the gene encoding Halobacterium halobium adenylate kinase." Gene. 175. 65-70 (1996)
[文献書誌] Go,M: "Site-specific mutagenesis of Phodobacter capsulatus Ferredoxin I,Fdx N,that functions in nitrogen fixation." Journal of Biological Chemistry. 271. 31339-31406 (1996)
[文献書誌] Go,M: "Mechanical stability of compact modules of barnase." FEBS Letters. 405. 47-54 (1997)
[文献書誌] H.Sakurai: "Function of reaction center of green sulfur bacteria." Photochemistry and Photobiology. 64. 5-13 (1996)
[文献書誌] S.Takaichi: "The major carotenoid in all known species of heliobacteria is the C30 carote Noid 4,4'-diaponeurosporene, neurosporene." Archives of Microbiology. 168. 277-281 (1996)
[文献書誌] Yoshinaga,K.: "Extensive RNA editing of U to C in addition to C to U substitution in the rbcL transcripts of hornwort chloroplasts and the origin of RNA editing in gren plants." Nucleic Acids Research. 24. 1008-1014 (1996)
[文献書誌] Ohta,N.: "Analysis of a plastid gene cluster reveals a close relationship between Cyanidioschyzon and Cyanidium." Journal of Plant Research. 110. 235-245 (1997)
[文献書誌] Mimuro,M.,: "Effect of molecular structure and solvents on the excited state dynamics of the S_2 state of carotenoids analyzed by means of the femtosecond up-conversion technique." Journal of American Chemical Society. 119. 1452-1453 (1997)
[文献書誌] Mimuro,M.: "Multiple pathways of the excitation energy flow in the phtosynthetic pigment system of a cryptophyta,Cryptomonas sp.(CR-1)." Phycological Research. (in press).
[文献書誌] Akimoto,S.: "Excitation energy transfer in carotenoid-chlorophyll protein complexes probed by femtosecond fluorescence decays." Chemical Physics Letters. 260. 147-152 (1996)
[文献書誌] Hori,K.: "A preliminary evidence that lectin-suger interaction exists in the initial recognition process in symbiosis involving dinoflagellates." Journal of Phycology. 32. 783-790 (1996)
[文献書誌] Nagashima,K.V.P.: "The nucleotide wequence of the puf operon from the purple photosynthetic bacterium,Phodospirillum molischianum:COmparative analysis of light-harvesting proteins and the cytochrome subunits associated with the reaction centers." Photosynthesis Research. 50. 61-70 (1996)
[文献書誌] Nagashima,K.V.P.: "Nucleotide sequences of genes coding for photosynthetic reaction centers and lightharvesting proteins of Acidiphilium rubrum and related aerobic acidophilic bacteria." Plant and Cell Physiology. 38. 1249-1258 (1997)
[文献書誌] H.Kikuchi: "Dynamical theory of the photoisomerization of rhodopsin chromophore:the generation of a transient electric field during the photoisomerization." Journal of Physical Chemistry,B,. 101. 6050-6056 (1997)
[文献書誌] H.Kikuchi: "An electronic state of the chromophore,phycocyanobilin,and its interaction with the protein moiety in C-phycocyanin;protonation of the chromophore." Chemicai Physical Letters. 274. 460-465 (1997)
[文献書誌] H.Kandori: "Real-time detection of 60-femtosecond isomerization in a rhodopsin analog containing 8-membered-ring-retinal." Journal of American Chemical Society. 118. 1002-1005 (1996)
[文献書誌] H.Imai,: "Single amino acid residue as a functional determinant of rod and cone pigments." Proceedings of National Academy of Science,USA. 94. 2322-2326 (1997)
[文献書誌] D.Kojima: "A novel Go-mediated phototransduction cascade in scallop visual cells" Journal of Biological Chemistry. 272. 22979-22982 (1997)
[文献書誌] Mimuro,M.: "Energy transfer processes in chlorosomes of a green photosynthetic bacterirum,Chloroflexus aurantiacus." Photosynthesis Research. 48. 263-270 (1996)
[文献書誌] Y.Fujiyoshi: "Three-Dimensional Structure of Aquaporin 1." Nature. 387. 624-627 (1997)
[文献書誌] Y.Fujiyoshi: "Surface of bacteriorhodopsin revealed by high-resolution electron crystallography." Nature. 389. 206-211 (1997)
[文献書誌] Y.Fujiyoshi: "The 3.0 Å Projection Structure of Microsomal Glutathione Transferase as Determined by Electron Crystallography of 21212 Two-dimensional Crystals." Journal of Molecular Biology. 271. 751-758 (1997)
[文献書誌] Amano,T.: "Catalytic Activities of α_3β_3γ Complexes of F1-ATPase with 1,2,or 3 Incompetent Catalytic Sites." Journal of Biological Chemistry. 271. 18128-18133 (1996)
[文献書誌] Hisabori,T.: "The Regulatory Functions of the g and e Subunits from Chloroplast CF1 are Tranaferred to the Core Complex,α_3β_3,from Thermophilic Bacterial F1." European Journal of Biochemistry. 247. 1158-1165 (1997)
[文献書誌] Kato,Y.: "Thermophilic F1-ATPase Is Activated without Dissociation of an Endogenoud Inhibitor,ε Subunit." Journal of Biological Chemistry. 272. 24906-24912 (1997)

1997 年度 実績報告書

蛋白質集合体の対称性の起源と系譜-機能的要請か、系統性か

研究代表者

三室 守 山口大学, 理学部, 教授 (40142004)

研究成果

[文献書誌] Tomioka,H.: "Voltage-dependent absorbance change of carotenoids in halophilic archaebacteria." Biochemica et Biophysica Acta. 1284. 79-85 (1996)

[文献書誌] Tomioka,H.: "Real-Time image processing using polarization discrimination of bacteriorhodopsin." Synthetic metals. 81. 147-149 (1996)

[文献書誌] Tomioka,H.: "Detection and expression of a gene encoding a novel bacteriorhodopsin from an extreme halophile strain HT(JCM9743)which possesses on bacteriorhopsin activity." Extremophiles. 2. 33-39 (1998)

[文献書誌] Go,M: "Cloning and characterization of the gene encoding Halobacterium halobium adenylate kinase." Gene. 175. 65-70 (1996)

[文献書誌] Go,M: "Site-specific mutagenesis of Phodobacter capsulatus Ferredoxin I,Fdx N,that functions in nitrogen fixation." Journal of Biological Chemistry. 271. 31339-31406 (1996)

[文献書誌] Go,M: "Mechanical stability of compact modules of barnase." FEBS Letters. 405. 47-54 (1997)

[文献書誌] H.Sakurai: "Function of reaction center of green sulfur bacteria." Photochemistry and Photobiology. 64. 5-13 (1996)

[文献書誌] S.Takaichi: "The major carotenoid in all known species of heliobacteria is the C30 carote Noid 4,4'-diaponeurosporene, neurosporene." Archives of Microbiology. 168. 277-281 (1996)

[文献書誌] Yoshinaga,K.: "Extensive RNA editing of U to C in addition to C to U substitution in the rbcL transcripts of hornwort chloroplasts and the origin of RNA editing in gren plants." Nucleic Acids Research. 24. 1008-1014 (1996)

[文献書誌] Ohta,N.: "Analysis of a plastid gene cluster reveals a close relationship between Cyanidioschyzon and Cyanidium." Journal of Plant Research. 110. 235-245 (1997)

[文献書誌] Mimuro,M.,: "Effect of molecular structure and solvents on the excited state dynamics of the S_2 state of carotenoids analyzed by means of the femtosecond up-conversion technique." Journal of American Chemical Society. 119. 1452-1453 (1997)

[文献書誌] Mimuro,M.: "Multiple pathways of the excitation energy flow in the phtosynthetic pigment system of a cryptophyta,Cryptomonas sp.(CR-1)." Phycological Research. (in press).

[文献書誌] Akimoto,S.: "Excitation energy transfer in carotenoid-chlorophyll protein complexes probed by femtosecond fluorescence decays." Chemical Physics Letters. 260. 147-152 (1996)

[文献書誌] Hori,K.: "A preliminary evidence that lectin-suger interaction exists in the initial recognition process in symbiosis involving dinoflagellates." Journal of Phycology. 32. 783-790 (1996)

[文献書誌] Nagashima,K.V.P.: "Nucleotide sequences of genes coding for photosynthetic reaction centers and lightharvesting proteins of Acidiphilium rubrum and related aerobic acidophilic bacteria." Plant and Cell Physiology. 38. 1249-1258 (1997)

[文献書誌] H.Kikuchi: "Dynamical theory of the photoisomerization of rhodopsin chromophore:the generation of a transient electric field during the photoisomerization." Journal of Physical Chemistry,B,. 101. 6050-6056 (1997)

[文献書誌] H.Kikuchi: "An electronic state of the chromophore,phycocyanobilin,and its interaction with the protein moiety in C-phycocyanin;protonation of the chromophore." Chemicai Physical Letters. 274. 460-465 (1997)

[文献書誌] H.Kandori: "Real-time detection of 60-femtosecond isomerization in a rhodopsin analog containing 8-membered-ring-retinal." Journal of American Chemical Society. 118. 1002-1005 (1996)

[文献書誌] H.Imai,: "Single amino acid residue as a functional determinant of rod and cone pigments." Proceedings of National Academy of Science,USA. 94. 2322-2326 (1997)

[文献書誌] D.Kojima: "A novel Go-mediated phototransduction cascade in scallop visual cells" Journal of Biological Chemistry. 272. 22979-22982 (1997)

[文献書誌] Mimuro,M.: "Energy transfer processes in chlorosomes of a green photosynthetic bacterirum,Chloroflexus aurantiacus." Photosynthesis Research. 48. 263-270 (1996)

[文献書誌] Y.Fujiyoshi: "Three-Dimensional Structure of Aquaporin 1." Nature. 387. 624-627 (1997)

[文献書誌] Y.Fujiyoshi: "Surface of bacteriorhodopsin revealed by high-resolution electron crystallography." Nature. 389. 206-211 (1997)

[文献書誌] Y.Fujiyoshi: "The 3.0 Å Projection Structure of Microsomal Glutathione Transferase as Determined by Electron Crystallography of 21212 Two-dimensional Crystals." Journal of Molecular Biology. 271. 751-758 (1997)

[文献書誌] Amano,T.: "Catalytic Activities of α_3β_3γ Complexes of F1-ATPase with 1,2,or 3 Incompetent Catalytic Sites." Journal of Biological Chemistry. 271. 18128-18133 (1996)

[文献書誌] Hisabori,T.: "The Regulatory Functions of the g and e Subunits from Chloroplast CF1 are Tranaferred to the Core Complex,α_3β_3,from Thermophilic Bacterial F1." European Journal of Biochemistry. 247. 1158-1165 (1997)

[文献書誌] Kato,Y.: "Thermophilic F1-ATPase Is Activated without Dissociation of an Endogenoud Inhibitor,ε Subunit." Journal of Biological Chemistry. 272. 24906-24912 (1997)

1997 年度実績報告書

三室守山口大学, 理学部, 教授 (40142004)