1999 年度実績報告書

膵癌の発生・増殖と進展及びその制御に関する基礎的研究

研究課題

研究課題/領域番号	08407009
研究機関	奈良県立医科大学
研究代表者	小西陽一奈良県立医科大学, 医学部, 教授 (00075061)
研究分担者	堤雅弘奈良県立医科大学, 医学部, 講師 (00207416) 傳田阿由美奈良県立医科大学, 医学部, 講師 (90110858) 中江大奈良県立医科大学, 医学部, 助教授 (90207712) 辻内俊文奈良県立医科大学, 医学部, 助手 (10254492)
キーワード	膵癌 / ハムスター / ヘテロサイクリックアミン / 発癌物質 / 化学予防
研究概要	平成11年度にハムスター膵癌実験系を用いて行った結果と進行状況について報告する。 1.平成9年度と10年度に見出されたTrp-P-1の膵管発癌に対するイニシエイションとプロモーション作用の知見をもとに,0.01%,0.02%Trp-P-1を経口投与し長期発癌実験を実施中で,現在実験開始後70週を経過している. 2.短期膵癌発生系における膵管上皮細胞のBrdu標識率を指標に,4.8-DimeIQXのイニシエイション活性を検索した結果,膵管上皮細胞のBrdu標識率の有意な上昇は見られず,イニシエイション活性はないと考えられた. 3.短期膵癌発生系におけるβ-カテニンの関与は否定された. 4.短期膵癌発生系において,0.1%トラネキサムサンは,動物1匹当たりの膵癌の発生個数を抑制した. 5.4-hydroxyphenyl retinamideとaurapteneは,それぞれ10μM,50μMの濃度でハムスター膵癌培養細胞にアポトーシスを誘導した.

研究成果

(18件)

すべてその他

すべて文献書誌 (18件)

[文献書誌] Sungki Choi: "Single treatment with cisplatin or UFT,but not their combination treatment enhances the metastic capacity of mouse fibrosarcoma cells"Anti-Cancer Drugs. 10. 235-243 (1999)
[文献書誌] Hiroyuki Sakitani: "Overpression of midkine in lumg tumors induced by N-nitrosobis(2-hydroxypropyl)amine in rats and its increase with progression"Carcinogenesis. 20. 465-469 (1999)
[文献書誌] Akira Kido: "Inhibition of Spontaneous Rat Osteosarcoma Lung Metastasis by 3S -[4-(N-hydroxyamino)-2R-isobutylsuccinyl]amino-1-methoxy-3,4-dihydrocarbostyril,a Novel Matrix Metalloproteinase Inhibitor"Jap.J.Cancer.Res.. 90. 333-341 (1999)
[文献書誌] 中江大: "実験的肝細胞癌の発生に関する研究の問題点"肝臓. 40. 61-65 (1999)
[文献書誌] Makoto Takahama: "Expression of Vascylar Endothelial Growth Factor and Its Receotprs during Lung Carcinogenesis by N-Nitorosobis(2-Hydroxypropyl)amine in Rats"Mol.Carcinogenesis. 24. 284-293 (1999)
[文献書誌] 堤雅弘: "膵癌の多段階発癌"Molecular Medicine. 36. 434-439 (1999)
[文献書誌] M Mori: "A Case-Control Study OnRisk Factors for Panceratic Diseases in Kerala,"Hepato-Gastroenterology. 46. 25-30 (1999)
[文献書誌] Akirara Kido: "Overexpression of materix metalloproteinase(MMP)-9 correlates with metastatic potency of spontaneous and 4-hydroxyaminoquinoline 1-oxide(4HAQO)-induced transplantable osteosarcomas in rats"Cancer Lett,. 137. 209-216 (1999)
[文献書誌] Ayimi Denda: "Geneteic properties for the suppression of development of putative preneplastic glutathione S-transferanse placental from-positive foci in the liver of carcinogen-resistant DRH stain rats"Cancer Lett,. 140. 59-67 (1999)
[文献書誌] Tomokazu Fukuda: "Cloning of differentially expressed genes in highly and low metastatic rat osteosarcomas by a modified DNA-AFLP method"Biochemical and Biophysical Research Communications. 261. 30-40 (1999)
[文献書誌] Toru Morishita: "Delay in administration of CDDP until completion of AGM-1470 treatment nhances antimetastatic and antitumor effects"Clinical & Experimental Metastasis. 17. 15-18 (1999)
[文献書誌] Masahiro Tsutsumi: "Inhibitory effects of combined administration of antibiotics and anti-inflammatory drugs on lung tu,or development initiated by N-nitrosobis(2-hydroxypropyl)amine in rats"Carcinogenesis. 21. 251-256 (1999)
[文献書誌] Makoto Takahama: "Enhanced expression of tie2,Its ligand angiopoietim-1,vascular endothelial growth factor,and CD31 in human non-small cell lung carcinomas"Clinical Cancer Research. 5. 1606-1510 (1999)
[文献書誌] Katsumichi Iki: "Expression of matrix metalloproteinase 2(MMP-2),membranetype 1 MMP andtissue inhibitor of metalloproteinase 2 and activation of proMMP-2 in pancreatic duct adenocarcinomas in hamsters treated with N-nitrosobis(2-oxopropyl)amine,Carcinogenesis"Carcinogenesis. 20. 1323-1329 (1999)
[文献書誌] Toshifumi Tsujiuchi: "Hypomethylation of CpG sites and c-myc gene overexpression in Hepatocellular carcinomas,but not Hyperplastic nodules,Induced by a Chloline-deficient L-amino acid-defined diet in rats"Jpn.J.Cancer.Res. 90. 909-913 (1999)
[文献書誌] Masatoshi Yoshimoto: "Carcinogenicity of heterocyclic amines for the pancreatic duct epithelium in hamsters"Cancer Lett. 143. 235-239 (1999)
[文献書誌] 堤雅弘: "切除膵臓癌における遺伝子異常"胆と膵. 20. 741-746 (1999)
[文献書誌] 堤雅弘: "膵臓発生の分子機構"消化器科. 30. 64-69 (1999)

1999 年度 実績報告書

膵癌の発生・増殖と進展及びその制御に関する基礎的研究

研究代表者

小西 陽一 奈良県立医科大学, 医学部, 教授 (00075061)

研究成果

[文献書誌] Sungki Choi: "Single treatment with cisplatin or UFT,but not their combination treatment enhances the metastic capacity of mouse fibrosarcoma cells"Anti-Cancer Drugs. 10. 235-243 (1999)

[文献書誌] Hiroyuki Sakitani: "Overpression of midkine in lumg tumors induced by N-nitrosobis(2-hydroxypropyl)amine in rats and its increase with progression"Carcinogenesis. 20. 465-469 (1999)

[文献書誌] Akira Kido: "Inhibition of Spontaneous Rat Osteosarcoma Lung Metastasis by 3S -[4-(N-hydroxyamino)-2R-isobutylsuccinyl]amino-1-methoxy-3,4-dihydrocarbostyril,a Novel Matrix Metalloproteinase Inhibitor"Jap.J.Cancer.Res.. 90. 333-341 (1999)

[文献書誌] 中江 大: "実験的肝細胞癌の発生に関する研究の問題点"肝臓. 40. 61-65 (1999)

[文献書誌] Makoto Takahama: "Expression of Vascylar Endothelial Growth Factor and Its Receotprs during Lung Carcinogenesis by N-Nitorosobis(2-Hydroxypropyl)amine in Rats"Mol.Carcinogenesis. 24. 284-293 (1999)

[文献書誌] 堤 雅弘: "膵癌の多段階発癌"Molecular Medicine. 36. 434-439 (1999)

[文献書誌] M Mori: "A Case-Control Study OnRisk Factors for Panceratic Diseases in Kerala,"Hepato-Gastroenterology. 46. 25-30 (1999)

[文献書誌] Akirara Kido: "Overexpression of materix metalloproteinase(MMP)-9 correlates with metastatic potency of spontaneous and 4-hydroxyaminoquinoline 1-oxide(4HAQO)-induced transplantable osteosarcomas in rats"Cancer Lett,. 137. 209-216 (1999)

[文献書誌] Ayimi Denda: "Geneteic properties for the suppression of development of putative preneplastic glutathione S-transferanse placental from-positive foci in the liver of carcinogen-resistant DRH stain rats"Cancer Lett,. 140. 59-67 (1999)

[文献書誌] Tomokazu Fukuda: "Cloning of differentially expressed genes in highly and low metastatic rat osteosarcomas by a modified DNA-AFLP method"Biochemical and Biophysical Research Communications. 261. 30-40 (1999)

[文献書誌] Toru Morishita: "Delay in administration of CDDP until completion of AGM-1470 treatment nhances antimetastatic and antitumor effects"Clinical & Experimental Metastasis. 17. 15-18 (1999)

[文献書誌] Masahiro Tsutsumi: "Inhibitory effects of combined administration of antibiotics and anti-inflammatory drugs on lung tu,or development initiated by N-nitrosobis(2-hydroxypropyl)amine in rats"Carcinogenesis. 21. 251-256 (1999)

[文献書誌] Makoto Takahama: "Enhanced expression of tie2,Its ligand angiopoietim-1,vascular endothelial growth factor,and CD31 in human non-small cell lung carcinomas"Clinical Cancer Research. 5. 1606-1510 (1999)

[文献書誌] Toshifumi Tsujiuchi: "Hypomethylation of CpG sites and c-myc gene overexpression in Hepatocellular carcinomas,but not Hyperplastic nodules,Induced by a Chloline-deficient L-amino acid-defined diet in rats"Jpn.J.Cancer.Res. 90. 909-913 (1999)

[文献書誌] Masatoshi Yoshimoto: "Carcinogenicity of heterocyclic amines for the pancreatic duct epithelium in hamsters"Cancer Lett. 143. 235-239 (1999)

[文献書誌] 堤 雅弘: "切除膵臓癌における遺伝子異常"胆と膵. 20. 741-746 (1999)

[文献書誌] 堤 雅弘: "膵臓発生の分子機構"消化器科. 30. 64-69 (1999)

1999 年度実績報告書

小西陽一奈良県立医科大学, 医学部, 教授 (00075061)

[文献書誌] 中江大: "実験的肝細胞癌の発生に関する研究の問題点"肝臓. 40. 61-65 (1999)

[文献書誌] 堤雅弘: "膵癌の多段階発癌"Molecular Medicine. 36. 434-439 (1999)

[文献書誌] 堤雅弘: "切除膵臓癌における遺伝子異常"胆と膵. 20. 741-746 (1999)

[文献書誌] 堤雅弘: "膵臓発生の分子機構"消化器科. 30. 64-69 (1999)