1997 年度実績報告書

動物用ポジトロン断層法による脳血流・代謝画像を用いた随意運動中枢制御機構の解明

研究課題

研究課題/領域番号	08558083
研究機関	京都大学
研究代表者	柴崎浩京都大学, 医学研究科, 教授 (30037444)
研究分担者	塚田秀夫浜松ホトニクス中央研究所, 研究員池田昭夫京都大学, 医学研究科, 助手 (90212761) 長峯隆京都大学, 医学研究科, 助手 (10231490) 福山秀直京都大学, 医学研究科, 助教授 (90181297) 米倉義晴福井医科大学, 高エネルギー研究所, 教授 (60135572)
キーワード	動物用PET / ブドウ糖代謝率 / 大脳皮質 / アセチルコリン / アルツハイマー病 / ハーダー腺 / アセチルコリンエステレース / 運動機構
研究概要	本研究の目的は,動物を用いて脳血流,脳ブドウ糖代謝率を測定し,運動時の一次感覚運動野,大脳基底核,小脳など運動関連領域の血流代謝の変化と同時に記録される運動関連電位などの電気生理学的データを相互に記録してその関連性を調べ,運動時の諸部位の役割を明らかにすることである.また,動物用3次元PETによる小動物(ラット,ネコ)でのデータ収集とその定量性に関する検討も行った. 動物用PETを用いて,サルにおいて,さまざまな強度による振動覚刺激で一次感覚運動野の刺激を行い,賦活時における脳血流を測定し,その増加がアセチルコリン阻害作用を有するスコポラミンで抑止され,しかも,アルツハイマー病治療に使われる可能性がある,タクリンなどアセチルコリンエステレース阻害薬で抑制されていた振動覚刺激による血流増加が復活することを明らかにし,動物用PETが将来の薬剤開発に有用であることを示した. 小動物のブドウ糖代謝率の測定を容易に行えるような高解像度の小型PET(SHR7700L)を浜松ホトニクスと共同開発した.本装置はラット,ネコ専用に使用すること,そのためガントリ-は回転しないようにし,垂直に固定すること,設置面積を小さくすることを目標に開発され,完成した.本装置は動物の生理状態や電気生理学的なデータの収集にきわめて適した装置となった. 2次元動物用PET(SHR2000)を用いて,ラットのブドウ糖代謝率の測定について詳細な検討を行った.ラットは前頭部下部にハーダー腺があり,デオキシグルコースの蓄積が脳以上に高い.したがって,前脳ブドウ糖代謝率の正確な測定に問題があることが指摘されていたが,ハーダー腺を除去したラットと正常ラットの前脳ブドウ糖代謝率の測定を行い,その影響がないことを明らかにした.また,ブドウ糖代謝率の測定で,ダイナミック法,スタティック法,パトラック法の3法を適応し,どの方法が最適な方法であるかも明らかにした.

研究成果

(40件)

すべてその他

すべて文献書誌 (40件)

[文献書誌] Dong,Y.et al.: "Assessment of benzodiazepine receptors using lodline-123-labeled lomazenil single-photon emission computed tomography in patients with ischemic cerebrovascular disease A comparison with PET study" Stroke. 28. 1776-1782 (1997)
[文献書誌] Fukuyama,H.et al.: "Brain functional activity during gait in normal subjects : A SPECT study." Neurosci Lett.288. 183-186 (1997)
[文献書誌] Fukuyama,H.et al.: "Effects of extracranial radioactivity on measurement of cerebral glucose metabolism by RAT-PET with [18F]-2-fluoro-2-deoxy-D-glucose." J.Cereb.Blood Flow Metab.17. 1261-1262 (1997)
[文献書誌] Hamano,T.et al.: "The cortical generators of the contingent negative variarion in humans : a study with subdural electrodes." Electroenceph.clin.Neurophysiol.104. 257-268 (1997)
[文献書誌] Hirano,S.et al.: "Cortical activation by monaural speech sound stimulation demonsrarted by positron emission tomography." Exp.Brain Res.113. 75-80 (1997)
[文献書誌] Hirano,S.et al.: "Cortical processing mechanism for vocalization with auditory verbal feedback" NeuroReport. 8. 2379-2392 (1997)
[文献書誌] Honda,M.et al.: "Movement-related cortical potential and regional cerebral blood flow change in patients with stroke after motor recovery" J.Neuro.Sci.146. 117-126 (1997)
[文献書誌] Ikeda,A.et al.: "Dissociation between contingent negative variation(CNV) and Bereitschaftspotential(BP) in patients with parkinsonism" Electroenceph.clin.Neurophysiol.102. 142-151 (1997)
[文献書誌] Nagahama,Y.et al.: "Cerebral glucose metabolism in corticobasal degeneration : Comparison with progressive supranuclear pasly and normal controls" Mov.Disord.12. 691-696 (1997)
[文献書誌] Nagamine,T.et al.: "Serial processing of the somesthetic information revealed by different effects of stimulus rate on the somatosensory evoked potentials and magnetic fields" Brain Res.(in press).
[文献書誌] Nishitani,N.et al.: "Cortical-hippocampal auditory prossing identified by magnetoencephalography" J.Cog.Neurosci.(in press).
[文献書誌] Shindo,K.et al.: "Clinical usefulness of dipole tracing method for localizing interictal spikes in partial epilepsy" Epilspsia. (in press).
[文献書誌] Tanaka,F.et al.: "Presurgical identification of epilepstic foci with iodine-123 iomazenil SPECT : comparison with brain perfusion SPECT and FDG-PET." Eur.J.Necl.Med.24. 27-34 (1997)
[文献書誌] Terada,K.et al.: "Familial cortical myoclomic tremor as a unique form of cortical reflex myoclonus" Mov.Disord.12. 370-377 (1997)
[文献書誌] Tsukada,H.et al.: "Functional activation of cerebral blood flow abolished by scopolamine is reversed by congnitive enhancers associated with cholinesterase inhibition : a positron emission tomography study in unanesthetized monkeys." J.Pharmacol.Exp.Ther.281. 1408-1414 (1997)
[文献書誌] Yamauchi,H.et al.: "Atrophy of the corpus callosum,cognitive impairment and cortical hypometabolism in progressive supranuclear palsy." Ann.Nurol.41. 606-614 (1997)
[文献書誌] Yamauchi,H.et al.: "Cerebral hematocrit decreases with hemodynamic compromise in carotid artery occlusion.A PET study." Stroke. 29. 98-103 (1998)
[文献書誌] Yamauchi,H.et al.: "Atrophy of the corpus callsum,cortical hypometabolism,and cognitive impairment in corticobasal degeneration." Arch Neurol.(in press).
[文献書誌] Yazawa,S.et al.: "Subdural recording of Bereitschaftspotential is useful for functional mapping of the epileptogenic motor area a case report." Epilepsia. 38. 245-248 (1997)
[文献書誌] Yazawa,S.et al.: "Cortical mechanism underlying externally cued gait initiation studied by contingent negative variation" Electroenceph.clin.Neurophysiol.105. 390-399 (1997)
[文献書誌] Yazawa,S.et al.: "Supplementary motor area is active in preparation for both voluntary muscle relaxation and contraction : subdual recording of Bereitschaftspotential" Neurosci.Lett.(in press).
[文献書誌] 葛谷聡他: "Jerk-locked back averaging(JLA)法によっててんかん発作時焦点が確認された補足運動野発作の一症例" 臨床神経. 37. 923-926 (1997)
[文献書誌] 杉剛直他: "安静閉眼時背景脳波の徐波特性定量判読" 医用電子と生体工学. 35. 95-99 (1997)
[文献書誌] 中村政俊他: "脳波モデルを介した徐波律動特性の定量解析" 医用電子と生体工学. 35. 392-399 (1997)
[文献書誌] Mikuni,N.et al.: ""Cavermous sinus" EEG : a new method for the preoperative evaluation of temporal lobe epilepsy." Epilepsia. 38. 472-482 (1997)
[文献書誌] Mikuni,N.et al.: "Frontopolar ictal epileptiform discharges on scale EEG in temporal lobe epilepsy" J.Clin.Neurophysiol.14. 507-512 (1997)
[文献書誌] Mima,T.et al.: "Human second somatosensory area : subdural and magnetoencephalographic recording of somatosensory evoked response" J.Neurol.Neurosurg.Psychiatry. 63. 501-505 (1997)
[文献書誌] Mima,T.et al.: "Afferent mechanism of cortical myoclonus studied by proprioception-related SEPs." Electroenceph.clin.Neurophysiol.104. 51-59 (1997)
[文献書誌] Mima,T.et al.: "Modality-specific organization for cutaneous and proprioceptive sense in human primary sensory cortex studied by chronic epicorotical recording" Electroenceph.clin.Neurophysiol.104. 103-107 (1997)
[文献書誌] Mima,T.et al.: "Pathogenesis of cortical myoclonus studied by magnetoencephalography" Ann.Neurol.(in press).
[文献書誌] Mima,T.et al.: "Cortical myoclonus : sensorimotor hyperexcitability" Neurology. (in press).
[文献書誌] Nagahama,Y.et al.: "Age-related changes in cerebral blood flow activation during a Card Sorting Test." Exp.Brain Res.114. 571-577 (1997)
[文献書誌] Ikeda,A.et al.: "Alopecia with carbamazepine in two patients with focal seizures." J Neurol Neurosurg Psychiatr.63. 549-550 (1997)
[文献書誌] Ikeda,A.et al.: "Scalp-recorded focal,ictal DC shift in a patient with tonic seizure" Epilepsia. 38. 1350-1354 (1997)
[文献書誌] Ikeda,A.et al.: "Reappraisal of the effects of electrode property on recording slow potentials." Electroenceph.clin.Neurophysiol.(in press).
[文献書誌] Ikeda,A.et al.: "Role of mesial teporal lobe in voluntary movements : Bereitschaftspotential study in epileptic patients after left antero-mesial temporal resection" Electroenceph.clin.Neurophysiol.(in press).
[文献書誌] Kojima,H.et al.: "The role of the temporal coding systems in the auditory cortex on speech recognition." NeuroReport. 8. 2395-2398 (1997)
[文献書誌] Lai,C.et al.: "Event-related potentials associated with judgment : Comparison of S1- and S2-choice conditions in a contingent negative variation (CNV) paradigm" J.Clin.Neurophysiol.14. 394-405 (1997)
[文献書誌] Lu,C.et al.: "Electrophysiological studies of early stage corticobasal degeneration" Mov.Disord.13. 140-146 (1997)
[文献書誌] Mikuni,N.et al: "Simultaneous recording of epileptiform discharges by MEG and subdural electrodes in temporal lobe epilepsy" Neuroimage. 5. 298-306 (1997)

1997 年度 実績報告書

動物用ポジトロン断層法による脳血流・代謝画像を用いた随意運動中枢制御機構の解明

研究代表者

柴崎 浩 京都大学, 医学研究科, 教授 (30037444)

研究成果

[文献書誌] Dong,Y.et al.: "Assessment of benzodiazepine receptors using lodline-123-labeled lomazenil single-photon emission computed tomography in patients with ischemic cerebrovascular disease A comparison with PET study" Stroke. 28. 1776-1782 (1997)

[文献書誌] Fukuyama,H.et al.: "Brain functional activity during gait in normal subjects : A SPECT study." Neurosci Lett.288. 183-186 (1997)

[文献書誌] Fukuyama,H.et al.: "Effects of extracranial radioactivity on measurement of cerebral glucose metabolism by RAT-PET with [18F]-2-fluoro-2-deoxy-D-glucose." J.Cereb.Blood Flow Metab.17. 1261-1262 (1997)

[文献書誌] Hamano,T.et al.: "The cortical generators of the contingent negative variarion in humans : a study with subdural electrodes." Electroenceph.clin.Neurophysiol.104. 257-268 (1997)

[文献書誌] Hirano,S.et al.: "Cortical activation by monaural speech sound stimulation demonsrarted by positron emission tomography." Exp.Brain Res.113. 75-80 (1997)

[文献書誌] Hirano,S.et al.: "Cortical processing mechanism for vocalization with auditory verbal feedback" NeuroReport. 8. 2379-2392 (1997)

[文献書誌] Honda,M.et al.: "Movement-related cortical potential and regional cerebral blood flow change in patients with stroke after motor recovery" J.Neuro.Sci.146. 117-126 (1997)

[文献書誌] Ikeda,A.et al.: "Dissociation between contingent negative variation(CNV) and Bereitschaftspotential(BP) in patients with parkinsonism" Electroenceph.clin.Neurophysiol.102. 142-151 (1997)

[文献書誌] Nagahama,Y.et al.: "Cerebral glucose metabolism in corticobasal degeneration : Comparison with progressive supranuclear pasly and normal controls" Mov.Disord.12. 691-696 (1997)

[文献書誌] Nagamine,T.et al.: "Serial processing of the somesthetic information revealed by different effects of stimulus rate on the somatosensory evoked potentials and magnetic fields" Brain Res.(in press).

[文献書誌] Nishitani,N.et al.: "Cortical-hippocampal auditory prossing identified by magnetoencephalography" J.Cog.Neurosci.(in press).

[文献書誌] Shindo,K.et al.: "Clinical usefulness of dipole tracing method for localizing interictal spikes in partial epilepsy" Epilspsia. (in press).

[文献書誌] Tanaka,F.et al.: "Presurgical identification of epilepstic foci with iodine-123 iomazenil SPECT : comparison with brain perfusion SPECT and FDG-PET." Eur.J.Necl.Med.24. 27-34 (1997)

[文献書誌] Terada,K.et al.: "Familial cortical myoclomic tremor as a unique form of cortical reflex myoclonus" Mov.Disord.12. 370-377 (1997)

[文献書誌] Tsukada,H.et al.: "Functional activation of cerebral blood flow abolished by scopolamine is reversed by congnitive enhancers associated with cholinesterase inhibition : a positron emission tomography study in unanesthetized monkeys." J.Pharmacol.Exp.Ther.281. 1408-1414 (1997)

[文献書誌] Yamauchi,H.et al.: "Atrophy of the corpus callosum,cognitive impairment and cortical hypometabolism in progressive supranuclear palsy." Ann.Nurol.41. 606-614 (1997)

[文献書誌] Yamauchi,H.et al.: "Cerebral hematocrit decreases with hemodynamic compromise in carotid artery occlusion.A PET study." Stroke. 29. 98-103 (1998)

[文献書誌] Yamauchi,H.et al.: "Atrophy of the corpus callsum,cortical hypometabolism,and cognitive impairment in corticobasal degeneration." Arch Neurol.(in press).

[文献書誌] Yazawa,S.et al.: "Subdural recording of Bereitschaftspotential is useful for functional mapping of the epileptogenic motor area a case report." Epilepsia. 38. 245-248 (1997)

[文献書誌] Yazawa,S.et al.: "Cortical mechanism underlying externally cued gait initiation studied by contingent negative variation" Electroenceph.clin.Neurophysiol.105. 390-399 (1997)

[文献書誌] Yazawa,S.et al.: "Supplementary motor area is active in preparation for both voluntary muscle relaxation and contraction : subdual recording of Bereitschaftspotential" Neurosci.Lett.(in press).

[文献書誌] 葛谷 聡 他: "Jerk-locked back averaging(JLA)法によっててんかん発作時焦点が確認された補足運動野発作の一症例" 臨床神経. 37. 923-926 (1997)

[文献書誌] 杉 剛直 他: "安静閉眼時背景脳波の徐波特性定量判読" 医用電子と生体工学. 35. 95-99 (1997)

[文献書誌] 中村政俊 他: "脳波モデルを介した徐波律動特性の定量解析" 医用電子と生体工学. 35. 392-399 (1997)

[文献書誌] Mikuni,N.et al.: ""Cavermous sinus" EEG : a new method for the preoperative evaluation of temporal lobe epilepsy." Epilepsia. 38. 472-482 (1997)

[文献書誌] Mikuni,N.et al.: "Frontopolar ictal epileptiform discharges on scale EEG in temporal lobe epilepsy" J.Clin.Neurophysiol.14. 507-512 (1997)

[文献書誌] Mima,T.et al.: "Human second somatosensory area : subdural and magnetoencephalographic recording of somatosensory evoked response" J.Neurol.Neurosurg.Psychiatry. 63. 501-505 (1997)

[文献書誌] Mima,T.et al.: "Afferent mechanism of cortical myoclonus studied by proprioception-related SEPs." Electroenceph.clin.Neurophysiol.104. 51-59 (1997)

[文献書誌] Mima,T.et al.: "Modality-specific organization for cutaneous and proprioceptive sense in human primary sensory cortex studied by chronic epicorotical recording" Electroenceph.clin.Neurophysiol.104. 103-107 (1997)

[文献書誌] Mima,T.et al.: "Pathogenesis of cortical myoclonus studied by magnetoencephalography" Ann.Neurol.(in press).

[文献書誌] Mima,T.et al.: "Cortical myoclonus : sensorimotor hyperexcitability" Neurology. (in press).

[文献書誌] Nagahama,Y.et al.: "Age-related changes in cerebral blood flow activation during a Card Sorting Test." Exp.Brain Res.114. 571-577 (1997)

[文献書誌] Ikeda,A.et al.: "Alopecia with carbamazepine in two patients with focal seizures." J Neurol Neurosurg Psychiatr.63. 549-550 (1997)

[文献書誌] Ikeda,A.et al.: "Scalp-recorded focal,ictal DC shift in a patient with tonic seizure" Epilepsia. 38. 1350-1354 (1997)

[文献書誌] Ikeda,A.et al.: "Reappraisal of the effects of electrode property on recording slow potentials." Electroenceph.clin.Neurophysiol.(in press).

[文献書誌] Ikeda,A.et al.: "Role of mesial teporal lobe in voluntary movements : Bereitschaftspotential study in epileptic patients after left antero-mesial temporal resection" Electroenceph.clin.Neurophysiol.(in press).

[文献書誌] Kojima,H.et al.: "The role of the temporal coding systems in the auditory cortex on speech recognition." NeuroReport. 8. 2395-2398 (1997)

[文献書誌] Lai,C.et al.: "Event-related potentials associated with judgment : Comparison of S1- and S2-choice conditions in a contingent negative variation (CNV) paradigm" J.Clin.Neurophysiol.14. 394-405 (1997)

[文献書誌] Lu,C.et al.: "Electrophysiological studies of early stage corticobasal degeneration" Mov.Disord.13. 140-146 (1997)

[文献書誌] Mikuni,N.et al: "Simultaneous recording of epileptiform discharges by MEG and subdural electrodes in temporal lobe epilepsy" Neuroimage. 5. 298-306 (1997)

1997 年度実績報告書

柴崎浩京都大学, 医学研究科, 教授 (30037444)

[文献書誌] 葛谷聡他: "Jerk-locked back averaging(JLA)法によっててんかん発作時焦点が確認された補足運動野発作の一症例" 臨床神経. 37. 923-926 (1997)

[文献書誌] 杉剛直他: "安静閉眼時背景脳波の徐波特性定量判読" 医用電子と生体工学. 35. 95-99 (1997)

[文献書誌] 中村政俊他: "脳波モデルを介した徐波律動特性の定量解析" 医用電子と生体工学. 35. 392-399 (1997)