腎疾患における高度糖化蛋白の病態生理学的意義に関する共同研究

1998 年度 研究成果報告書概要

• 研究課題/領域番号: 09044280
• 研究種目: 国際学術研究
• 配分区分: 補助金
• 応募区分: 共同研究
• 研究分野: 腎臓内科学
• 研究機関: 東海大学
• 研究代表者: 宮田 敏男 東海大学, 総合医学研究所, 講師 (10222332)
• 研究分担者: VAN Ypersele ルパイン大学, 医学部, 教授 ; JOHN W. Bayn サウスカロライナ大学, 医学部, 教授 ; STERN David コロンビア大学, 細胞生理学部, 教授 ; MONNIER Vinc ケースウエスタンリザーブ大学, 病理研究所, 教授 ; BAYNES John W. University of South Carolina, Professor ; C.VAN Ypersele de Striho University of Louvain Medical School, Professor
• 研究期間 (年度): 1997 – 1998
• キーワード: 酸化ストレス / カルボニルストレス / AGEs / 腎不全 / レドックス制御 / 糖尿病 / ペントシジン

研究概要

糖酸化反応経路の解明を目的にその前駆物質・中間体の分析を行い、従来種々のレドックス異常のもと、蛋白の糖酸化反応により生成が亢進するとされてきたAGEsが、蛋白と脂質の反応からも生成されることが判明し、このような条件下では脂質過酸化物による蛋白修飾物でのALEsの生成も亢進することを示した。そしてAGEs/ALEsの多くは複数の反応性の高いカルボニル化合物からそれぞれ独立に生成されうることを証明した。また、血清及び組織蛋白中に存在するいくつかの反応性カルボニルによる蛋白修飾物の高速液体クロマトグラフィ、ガスクロマトグラフィ・マススペクトメトリー法などによる化学的同定・定量を確立し、腎不全、糖尿病、動脈硬化性疾患、慢性関節リウマチなどの血中の反応性カルボニルによる蛋白修飾物の構造の解析を行った。その代表的化合物であるペントシジン、カルボキシメチルリジン(CML)、マロンジアルテヒド(MDA)およびヒドロキシノネナール(HNE)を同定・定量した。さらに反応性カルボニルによる蛋白修飾物の特異的抗体の作製を行い、そのうち抗ペントシジン抗体の作製を完了し、抗ペントシジン抗体を用いたELISA定量系を確立し、現在それ以外の抗体を用いた定量系の開発が進行している。
反応性カルボニルの蛋白修飾物の特異的抗体を用いた免疫組織化学的な解析が進行し、種々の病変部局所の組織蛋白中にこれらの化合物が存在することを明らかにした。特に、代表的な長期透析合併症である透析アミロイドーシスの骨関節部に沈着しているアミロイドにAGEsが沈着していることを見出し、透析アミロイドーシスの病因にカルボニルストレスが関連していることを指摘した。

研究成果

• [文献書誌] Toshio Miyata: "Advanced glycation end products enhance osteoclast-induced bone resorption in cultured mouse unfractionated bone cells and in rats implanted with devitalized bone particles" J Am Soc Nephrol. 8. 260-270 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Oxidation conspires with glycation to create noxious advanced glycation end products in renal failure" Nephrol Dial Transplant. 12. 255-258 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Clearance of pentosidine, an advanced glycation end product, by different modalities of renal replacement therapy" Kidney International. 51. 880-887 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Implication of redox imbalance in accelerated formation of advanced glycation end products in renal failure patients." Kidney International. 51. 1170-1181 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Immunohistochemical localization of advanced glycation end products, pentosidine and carboxymetyllysine, inlipofuscin pigments of alzheimer' s disease and aged neurons" Biochem Biophys Res Commun. 232. 327-332 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Protein modification by a Maillard reaction intermediate methylglyoxal immunochemical detection of fluorescent 5-methylimidazolone derivatives in vivo." FEBS Letter. 410. 313-318 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Advanced glycation endproducts stimulate mitogen-activated protein kinase and proliferation in rabbit vascular smooth muscle cells." Biochem Biophys Res Commun. 239. 111-115 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Immunohistochemical colocalization of glycoxidation products and lipid peroxidation products in diabetic renal glomerular lesions." J Clin Invest. 100. 2995-3004 (1997)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Funcional quantiative analysis of the genome in cultured human mesangial cells." Kidney International. 53. 154-158 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Renal catabolism of advanced glycation end products : the fate of pentosidne." Kidney International. 53. 416-422 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Increased pentosidine, an davanced glycation end products, in plasma and synovial fluid from patients with rheumatoid arthritis and its relation with markers of inflammation." Biochem Biophys Res Commun. 244. 45-49 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Stress carbonyle et complications a long terme de I uremie" ACTUALITES NEPHROLOGIQUES JEAN HAMBURGER, HoPITAL NECKER 1998. 247-253
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Protein-bound acrolein : potential markers for oxidative stress." Proc Natl Acad Sci USA. 95. 4882-4887 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Alteraitions of synovial tissue and their potential rolu in the deposition of β_2-microglobulin-associated amyloid." Nephrol Dial Transplant. 13. 1465-1475 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "β2-Microgloburin in renal disease." J Am Soc Nephrol. 9. 1723-1735 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Implication of the glycoxidation and lipoxidation rections in the pathogenesis of dialysis-related amyloidosis." Int J Mol Med. 2. 561-565 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Plasma level of pentosidine in diabetic : an advanced glycation end product." J Am Soc Nephrol. 9. 1681-1688 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Implication of altered redox regulation by antioxidant enzymes in the increased plasma pentosidine, an advanced glycation end product in uremia." Biochem Biophys Res Commun. 245. 785-790 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Autoxidation products of both carbohydrates and lipids are increased in uremic plasma : Is there oxidative stress in uremia?" Kidney International. 54. 1290-1295 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "A mesangium-predominant gene, megsin is a new serpin up-regulated in IgA nephropathy." J Clin Invest. 102. 828-836 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Accelerated formation of carboxymethyllysine, an advanced glycation end product, by glyoxal and 3-deoxyglucosone in cultured ratsensoryneurons" Biochem Biophys Res Commun. 248. 93-97 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Advanced glycation end products co-localization with astoricytes and microglial cells in Alzheimer' s disease brain." Acta Neuropathol. 98. 555-558 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Generation of protein carbonyls by glycation and lipoxidation reactions with autoxidation products of ascorbic acid and polyunsaturated fatty acids." FEBS letter. 437. 24-28 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Localization of identified advanced glycation end product structure-carboxymethyllysine and pentosidine, in age-related inclusions in humanbrains" Pathology International. 48. 575-579 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Level of HgC_2-meditated phosphorylaiton of intracellular proteins determines death of thymic T-lymphocytes with or without DNA fragmentation." J Cellular Biochemistry. 71. 243-253 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress in uremia : Accumulation of carbonyls accelerate the formation of pentosidine, an advanced glycation end product." J Am Soc Nephrol. 9. 2349-2356 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl compounds cross-link cellular proteins and activate protein-tyrosine kinase p60_<c-src>." J Cellular Biochemistry. 72. 1-7 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Alterations in non-enzymatic biochemistry in uremia : Origin and significance of “carbonyl stress" in long-term uremic complications." Kidney International. 55. 389-399 (1998)
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress : increased carbonyl modification of proteins by autoxidation products of carbohydrates and lipids." Int J Art Org. in-press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Possible involvement of “carbonyl stress" in the pathogenesis of dialysis-related amyloidosis." Am J Med Sci. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Rapid formation of advanced glycation end products following vascular injury in euglycemic rat carotid artery : Role of oxidative stress." Circulation Research. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Oxidative stress-induced “carbonyl stress" in uremia." Redox Regulation Clinical Application. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "“Carbonyl Stress" and long-term uremic complications." Advanced in Nephrology. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress in long-term uremic complications." Nephrol Dial Transplant. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "“Carbonyl Stress" and dialysis-related amyloidosis." Nephrol Dial Transplant. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Immunohistochemical evidence for an increased oxidative stress and carbonyl modification of proteinsby autoxidation products of carbohydrates, lipids, and amino acids in diabetic glomerular lesions" J Am Soc Nephorl. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress : Increased carbonyl modification of tissue and cellular proteins in uremia." Peritoneal Dialysis International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "β2-microglobulin renal bone disease." Peritoneal Dialysis International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "A sensitive and specific ELISA for plasma pentosidine." Nephrol Dial Transplant. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Neurotoxicity methylglyxal and 3-Deoxyglycose on cultured cortical neurons : Synergism between glycation and oxidative stress, possibly involved in neurodegnerative diseases." Neurosci letter. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Development of a polyclonal antibody with broad epitope specificityy for advancedglycation endproducts and its use for the detection of AGEs in aging human Bruch' s membrane and choroid." Experimental Eye Research. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Infuluence of hemodialysis membrane type on pentosidine plasma level, a marker of “carbonyl stress"." Kidney International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress diabetic nephropathy." Jpn J Nephrol. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Implication of carbonyl stress in long-term uremic complications." Blood Purification. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "The first Japan-European Nephrology Forum." Nephorl Dial Transplant. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Increased carbonyl modification of proteins by lipids and carbohydrates in diabetic nephropathy." Kidney International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Increase of the advanced glycation endproduct pentosidine Bruchs membrane with age." Investigative Ophthalmology Visual Science. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Expression of megsin mRNA, a novel mesangium-predominant gene, in the renal tissues of various glomerular diseases." J Am Soc Nephrol. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Increased oxidative stress in the mouse kidneys with unilateral ureteral obstruction." Kidney International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "2-Isopropylidenehydrazono-4-oxo-thiazolidin-5ylacetanilide (OPB-9195) inhibits the neointima proliferation of rat carotid artery following balloon injury : Role of glycoxidation and lipoxidation reactions in vascular tissue damage." FEBS letter. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "2-Isopropylidenehydrazono-4-oxo-thiazolidin-5ylacetanilide (OPB-9195) is a potent inhibitor for glycoxidation and lipoxidation reactions." Biochem Biophys Res Commun. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Apo E2/E5 mutation in lipoprotein glomerulopathy recurred in transplanted kidnev." J Am Soc Nephrol.in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress in chronic renal failure (Nephrology Forum)." Kidney International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Relevance of oxidative stress in long-term complicatios in hemodialysis." Kidney International. in press.
  • 説明: 「研究成果報告書概要(和文)」より
• [文献書誌] Toshio Miyata: "Advanced glycation end products enhance osteoclast-induced bone resorption in cultured mouse unfractionated bone cells and in rats implanted with devitalized bone particles." J Am Soc Nephrol. 8. 260-270 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Oxidation conspires with glycation to create noxious advanced glycation end products in renal failure." Nephrol Dial Transplant. 12. 255-258 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Clearance of pentosidine, an advanced glycation end product, by different modalities of renal replacement therapy." Kidney International. 51. 880-887 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Implication of redox imbalance in accelerated formation of advanced glycation end products in renal failure patients." Kidney International. 51. 1170-1181 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Immunohistochemical localization of advanced glycation end products, pentosidine and carboxymetyllysine, inlipofuscin pigments of alzheimer's disease and aged neurons." Biochem Biophys Res Commun. 236. 327-332 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Protein modification by a maillard reaction intermediate methylglyoxal immunochemical detection of fluorescent 5-methylimidazolone derivatives in vivo." FEBS Letter. 410. 313-318 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Advanced glycation endproducts stimulate mitogen-activated protein kinase and proliferation in rabbit vascular smooth muscle cells." Biochem Biophys Res Commun. 239. 111-115 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Immunohistochemical-colocalization of glycoxidation products and lipid peroxidation products in diabetic renal glomerular lesions." J Clin Invest. 100. 2995-3004 (1997)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Functional quantiative analysis of the genome in cultured human mesangial cells." Kidney International. 53. 154-158 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Renal catabolism of advanced glycation end product s : the fate of pentosidne." Kidney International. 53. 416-422 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Increased pentosidine, an advanced glycation end products, in plasma and synovial fluid from patients with rheumatoid arthritis and its relation with markers of inflammation." Biochem Biophys Res Commun. 244. 45-49 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Stress carbonyle et complications a long terme de I' uremie" ACTUALITES NEPHROLOGIQUES JEAN HAMBURGER.HOPITAL NECKER. (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Protein-bound acrolein : potential markers for oxidative stress." Proc Natl Acad Sci USA. 95. 4882-4887 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Alteraitions of synovial tissue and their potential rolu in the deposition of beta_2-microglobulin-associated amyloid." Nephrol Dial Transplant. 13. 1465-1475 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "beta2-Microgloburin in renal disease." J Am Soc Nephrol. 9. 1723-1735 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Implication of the glycoxidation and lipoxidation rections in the pathogenesis of dialysis-related amyloidosis." Int J Mol Med. 2. 561-565 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Plasma level of pentosidine in diabetic patients : an advanced glycation end product." J Am Soc Nephrol. 9. 1681-1688 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Implication of altered redox regulation by antioxidant enzymes in the increased plasma pentosidine, an advanced glycation end product, in uremia." Biochem Biophys Res Commun. 245. 785-790 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Autoxidation products of both carbohydrates and lipids are increased in uremic plasma : Is there oxidative stress in uremia?" Kidney International. 54. 1290-1295 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "A mesangium-predominant gene, megsin, is a new serpin up-regulated in IgA nephropathy." J Clin Invest. 102. 828-836 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Accelerated formation of carboxymethyllysine, an advanced glycation end product by glyoxal and 3-deoxyglucosone in cultured rat sensory neurons." Biochem Biophys.Res Commun. 248. 93-97 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Advanced glycation end products co-localization with astoricytes and microglial cells in Alzhimer's disease brain." Acta Neuropathol. 98. 555-558 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Generation of protein carbonyls by glycation and lipoxidation reactions with autoxidation products of ascorbic acid and polyunsaturated fatty acids." FEBS letter. 437. 24-28 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Localization of identified advanced glycation end product structure, N-carboxymethyllysine and pentosidine, in age-related inclusions in human brains." Pathology International. 48. 575-579 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Level of HgCl_2-mediated phosphrylaiton of intracellular proteins determines death of thymic T-lymphocytes with or-without DNA fragmentation." J Cellular Biochemistry. 7. 243-253 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress in uremia : Accumulation of carbonyls accelerate the formation of pentosidine, an advanced glycation end product." J Am Soc Nephrol. 9. 2349-2356 (1998)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl compounds cross-link cellular proteins and activate protein-tyrosine kinase p60^<c-src>" J Cellular Biochemistry. 72. 1-7 (1999)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Alterations in non-enzymatic biochemistry in uremia : Origin and significance of "carbonyl stress" in long-term uremic complications." Kidney International. 55. 389-399 (1999)
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress : increased carbonyl modification of proteins by autoxidation products of carbohydrates and lipids." Int J Art Org. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Possible involvement of "carbonyl stress" in the pathogenesis of dialysis-related amyloidosis." Am J Med Sci. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Rapid formation of advanced glycation end products following vascular injury in euglycemic rat carotid artery : Role of oxidative stress." Circulation Research. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Oxidative stress-induced "carbonyl stress" in uremia." Redox Regulation Clinical Application. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: ""Carbonyl Stress" and long-term uremic complications." Advanced in Nephrology. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress in long-term uremic complications." Nephrol Dial Transplant. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: ""Carbonyl stress" and dialysis-related amyloidosis." Nephrol Dial Transplant. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Immunohistochemical evidence for an increased oxidative stress and carbonyl modification of proteins by autoxidation products of carbohydrates, lipids, and amino acids in diabetic glomerular lesions." J Am Soc Nephrol. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress : Increased carbonyl modification of tissue and cellular proteins in uremia." Peritoneal Dialysis International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "beta2-microglobulin and renal bone disease." Peritoneal Dialysis International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "A sensitive and spcific ELISA for plasma pentosidine." Nephrol Dial Transplant. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Neurotoxicity of methylglyxal and 3-Deoxyglycose on cultured cortical neurons : Synergism between glycation and oxidative stress, possibly involved in neurodegnerative diseases." Neurosci letter. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Development of a polyclonal antibody with braod epitope specificity for advanced glycation endproducts and its use for the detection of AGEs in aging human Bruch's membrane and choroid." Experimental Eye Research. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Infuluence of hemodialysis membrane type on pentosidine plasma level, a marker of "carbonyl stress"." Kidney International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress and diabetic nephropathy." Jpn J Nephrol. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Implication of carbonyl stress in long-term uremic complications." Blood Purification. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "The first Japan-European Nephrology Forum." Nephrol Dial Transplant. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Increased carbonyl modification of proteins by lipids and carbohydrates in diabetic nephropathy." Kidney International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Increase of the advanced glycation endproduct pentosidine in Bruch's membrane with age." Investigative Ophthalmology. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Expression of megsin mRNA,a novel mesangium-predominant gene, in the renal tissues of various glomerular diseases." J Am Soc Nephrol. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Increased oxidative stress in the mouse kidneys with unilateral ureteral obstruction." Kidney International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "2-Isopropylidenehydrazono-4-oxo-thiazolidin-5ylacetanilide (OPB-9195) inhibits the neointima proliferation of rat carotid artery following balloon injury : Role of glycoxidation and lipoxidation reactions in vascular tissue damage." FEBS letter. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "2-Isopropylidenehydrazono-4-oxo-thiazolidin-5ylacetanilide (OPB-9195) is a potent inhibitor for glycoxidation and lipoxidation reactions." Biochem Biophys Res Commun. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Apo E2/E5 mutation lipoprotein glomerulopathy recurred in transplanted kidney." J Am Soc Nephrol.(in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Carbonyl stress in chronic renal failure (Nephrology Forum)." Kidney International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より
• [文献書誌] Toshio Miyata: "Relevance of oxidative stress in long-term complications in hemodialysis." Kidney International. (in press).
  • 説明: 「研究成果報告書概要(欧文)」より