1998 年度実績報告書

発がんの化学予防

研究課題

研究課題/領域番号	09253104
研究種目	特定領域研究(A)
研究機関	京都府立医科大学
研究代表者	西野輔翼京都府立医科大学, 医学部, 教授 (10079709)
研究分担者	高須賀信夫国立がんセ化学療法部, 主任研究者 (80270677) 長沢弘明治大学, 農学部, 教授 (10076987) 山本智理子〔財〕癌研究会癌研究所, 病理部, 研究員 (10280629) 大東肇京都大学, 大学院・農学研究科, 教授 (80026583) 奥山徹明治薬科大学, 薬学部, 教授 (40087789)
キーワード	化学予防 / 天然カロテノイド / イソリクイリチゲニン / グリチルレチン酸モノグルクロナイド / タイ国産野菜 / d-リモネン / 一酸化窒素 / WAF-1
研究概要	1. 種々の天然カロテノイドが発がんの化学予防に有用であることを示す多くのデータが動物実験によって得られた。特に、リコピン(トマトに豊富に含まれているカロテノイド)は、有用性が高いと判定された。 2. ラッキョウ、アルファルファ、甘草等に含まれているイソリクイリチゲニンが、AOMで誘発される大腸粘膜異常腺窩巣(ACF)の形成を有意に抑制することが明らかとなった。 3. 新しい甘味科として開発されたグリチルレチン酸モノグルクロナイド(MGGR)が優れた肺発がん抑制効力を持つことを証明した。 4. タイ国産野菜の一種であるヨモギナから単離され、AL-1と命名された化合物が皮膚発がんプロモーションを抑制することが明らかとなった。 5. ヒト正常型c-Ha-ras遺伝子トランスジェニックラット(Tgラット)における乳腺発がんをカンキツ類に含まれているd-リモネンが抑制することを確認できた。 6. 一酸化窒素(NO)の発がんイニシエーター作用を、クルクミンおよび薬用人参抽出物が抑制することを証明した。 7. 大腸発がんの抑制効果があることが示唆されているブチレートは、WAF-1遺伝子プロモーター領域に存在するSp1-3部位を介して、WAF-1の発現を促進し、細胞周期のG1期において増殖停止を誘導することが明らかとなった。

研究成果
(32件)

すべてその他

すべて文献書誌 (32件)

[文献書誌] Nishino,H: "Cancer prevention by carotenoids." Mutation Res.402. 159-163 (1998)
[文献書誌] Nishino,H: "Inhibitory effect of tomato juice on rat urinary bladder carcinogenesis after N-buty-N-(4-hydroxxybutyl)nitrosamine initiation." Jpn.J.Cancer Res.89. 22-26 (1998)
[文献書誌] Nishino,H: "Inhibitory effect of beta-carotene,palm caroteine,and green polyphenols on pancreatic carcinogenesis initiated by N-nitorsobis(2-oxopropyl)amine in Syrian golden hamsters." Pancreas.16. 13-18 (1998)
[文献書誌] Nishino,H: "Inhibitory effect of fatty acid monoesters of 2-0-b-D-glucosylglycerol on Epstein-Barr virus activation." Cancer Lett.123. 83-86 (1998)
[文献書誌] Nishino,H: "Chemopreventive effect of carotenoids and curcumins on mouse colon carcinogenesis after 1,2-dimethylhydrazine initiation" Carcinogenesis. 19. 81-85 (1998)
[文献書誌] Okuyama,T: "Studies on cancer chemoprevention by traditionai folk medicines.XVI.Anti-tumor promoting activities of Kampo prescriptions used for Oketsu syndrome" Natural Medicines. 52. 140-144 (1998)
[文献書誌] Okuyama,T: "Studies on cancer chemoprevention by traditional folk medicines.XVIII.Anti-tumor promoting activity of Araliaceous plants." Natural Medicines. 52. 314-321 (1998)
[文献書誌] Hayatsu,H: "Iron chlorophyllin-mediated conversion of 3-hydroxyamino-1-methyl-5H-pyrido〔4,3-b〕indole(Trp-P-2(NHOH)into its nitroso derivative." Mutation Res.400. 259-269 (1998)
[文献書誌] Hayatsu,H: "Use of activated heterocyclic amines in the screening of dietary antimutagens" Mutation Res.402. 225-230 (1998)
[文献書誌] Hayatsu,H: "Inhibition of N-nitrosation of secondary amines in vitro by tea extracts and catechins." Mutation Res.412. 91-98 (1998)
[文献書誌] Hayatsu,H: "Chemopreventive properties of chloropbylls towards aflatoxin B1:a review of the antimutagenicity and anticarcinogenicity data in rainbow trout." Mutaion Res.399. 245-253 (1998)
[文献書誌] Mori H: "Inhibitory effects of nabumetone,a cyclooxygenase-2 inhibitor,and esculetin a lipoxygenase inhibitor,on N-methyl-N-nitrosourea-induced mammary carcinogenesis in rats." Jpn.J.Cancer Res.89. 496-501 (1998)
[文献書誌] Nagasawa,H: "Effects of Sasa Health^R,extract of bamboo leaves,on spontaneous mammary tumourigenesis in SHN mice" Anticancer Research. 18. 153-158 (1998)
[文献書誌] Nagasawa,H: "Effects of ovariectomy and prolactin on mammry gland expression of transforming growth factor and epidermal growth factor receptor mRNAs in mice" Zoological Science. 15. 525-529 (1998)
[文献書誌] Denda,A: "Chemoprevention effects of nimesulide,a selective cyclooxygenease-2 inhibitor,on the development of rat urinary bladder carcinomas intiated by N-buty-N-(4-hydroxybutyl)nitrosamine." Cancer Research.58. 3028-3031 (1998)
[文献書誌] Denda.A: "Prevention by inhibitors of arachidonic acid cascade of liver carcinogenesis,cirrhosis and oxidative DNA damage caused by a choline-deficient L-amino acid-defined diet in rats." Mutation Research. 402. 279-288 (1998)
[文献書誌] Denda,A: "Inhibition by phenyl N-tert-butyl nitrone of early phase carcinogenesis in the livers of rats fed a cholinc-deficient,L-amino acid defined diet." Cancer Res.58. 4548-4551 (1998)
[文献書誌] Denda,A: "Prevention by 1'-acetoxychavicol acetate of the induction but not growth of putative preneoplastic glutathione S-transferase placental form-positive focal lesision in thelivers of rats fed a choline-deficient,L-amino acid-defined diet." Carcinogenesis. 19. 1809-1814 (1998)
[文献書誌] Denda,A: "Inhibition of early-phase exogenous and endogenous liver carcinogenesis in transgenic rats harboring a rat glutathione S-transferase placental form gene." Jpn.J Cancer Res.89. 1118-1125 (1998)
[文献書誌] Denda,A: "Induction of telomerase activity during regeration after partial hepatectomy in the rat." Cander Letter. 122. 115-120 (1998)
[文献書誌] Ohigashi,H: "Inhibitory effects of curcumin and tetrahydrocurcuminoids on the tumor promoter-induced reactive oxygen species generation in leukocytes in vitro and in vivo." Jpn.J.Cancer Res.89. 361-370 (1998)
[文献書誌] Ohigashi,H: "Superoxide scvenging activity of rosmarinic acid from Perilla frutescens Britton var acuta f vifridis" J.Agric.Food Chem.46. 4545-4550 (1998)
[文献書誌] Ohigashi,H: "Screening for in vitro anti-tumor-promoting activities of edible plants from Indonesia." Cancer Detec.Prev.22. 516-525 (1998)
[文献書誌] Ohigashi,H: "Sereening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 246.7 cells." Cancer Lett.125. 199-207 (1998)
[文献書誌] Ohigashi,H: "Niaziminin,a thiocarbamate from the leaves of Moringa oleifera,holds a strict structural requirement for inhibition of tumor-promoter-induced Epstein-Barr virus activation." Planta Med.64. 319-323 (1998)
[文献書誌] Fujiki,H: "Wide distribution of ^3H-(-)-epigallocatechin gallate,a cancer preventive tea polyphenol,in mouse tissue" Carcinogen. 19. 1771-1776 (1998)
[文献書誌] Fujiki,H: "Cancer inhibition by green tea" Mutat.Res.402. 307-310 (1998)
[文献書誌] Fujiki,H: "Molecular pathogenesis of interstitial pneumonitis with TNF-a transgenic mice" Cytokine. 10. 124-131 (1998)
[文献書誌] Sakai,T: "Promotoer activation and following induction of the p21/WAF1 gene by flavone is involved in G1 phase arrest in A549 Iung adenocarcinoma cells." FEBS Lett.437. 61-64 (1998)
[文献書誌] Sakai,T: "p53 independent activation of the gadd45 promoter by Δ^<12>-prostaglandin J_2" Biochem.Biophys.Res.Commun.251. 648-652 (1998)
[文献書誌] Sakai,T: "p53-independent induction of WAF1/Cip is correlated with osteoblastic differentiation by vitamin D3." Cancer Lett.129. 61-68 (1998)
[文献書誌] Sakai,T: "Cell cycle-dependent modulation of promoter activities of RB and WAF1/Cip1 genes." Jpn.J.Cancer Res.89. 626-633 (1998)

1998 年度 実績報告書

発がんの化学予防

研究代表者

西野 輔翼 京都府立医科大学, 医学部, 教授 (10079709)

研究成果

[文献書誌] Nishino,H: "Cancer prevention by carotenoids." Mutation Res.402. 159-163 (1998)

[文献書誌] Nishino,H: "Inhibitory effect of tomato juice on rat urinary bladder carcinogenesis after N-buty-N-(4-hydroxxybutyl)nitrosamine initiation." Jpn.J.Cancer Res.89. 22-26 (1998)

[文献書誌] Nishino,H: "Inhibitory effect of beta-carotene,palm caroteine,and green polyphenols on pancreatic carcinogenesis initiated by N-nitorsobis(2-oxopropyl)amine in Syrian golden hamsters." Pancreas.16. 13-18 (1998)

[文献書誌] Nishino,H: "Inhibitory effect of fatty acid monoesters of 2-0-b-D-glucosylglycerol on Epstein-Barr virus activation." Cancer Lett.123. 83-86 (1998)

[文献書誌] Nishino,H: "Chemopreventive effect of carotenoids and curcumins on mouse colon carcinogenesis after 1,2-dimethylhydrazine initiation" Carcinogenesis. 19. 81-85 (1998)

[文献書誌] Okuyama,T: "Studies on cancer chemoprevention by traditionai folk medicines.XVI.Anti-tumor promoting activities of Kampo prescriptions used for Oketsu syndrome" Natural Medicines. 52. 140-144 (1998)

[文献書誌] Okuyama,T: "Studies on cancer chemoprevention by traditional folk medicines.XVIII.Anti-tumor promoting activity of Araliaceous plants." Natural Medicines. 52. 314-321 (1998)

[文献書誌] Hayatsu,H: "Iron chlorophyllin-mediated conversion of 3-hydroxyamino-1-methyl-5H-pyrido〔4,3-b〕indole(Trp-P-2(NHOH)into its nitroso derivative." Mutation Res.400. 259-269 (1998)

[文献書誌] Hayatsu,H: "Use of activated heterocyclic amines in the screening of dietary antimutagens" Mutation Res.402. 225-230 (1998)

[文献書誌] Hayatsu,H: "Inhibition of N-nitrosation of secondary amines in vitro by tea extracts and catechins." Mutation Res.412. 91-98 (1998)

[文献書誌] Hayatsu,H: "Chemopreventive properties of chloropbylls towards aflatoxin B1:a review of the antimutagenicity and anticarcinogenicity data in rainbow trout." Mutaion Res.399. 245-253 (1998)

[文献書誌] Mori H: "Inhibitory effects of nabumetone,a cyclooxygenase-2 inhibitor,and esculetin a lipoxygenase inhibitor,on N-methyl-N-nitrosourea-induced mammary carcinogenesis in rats." Jpn.J.Cancer Res.89. 496-501 (1998)

[文献書誌] Nagasawa,H: "Effects of Sasa Health^R,extract of bamboo leaves,on spontaneous mammary tumourigenesis in SHN mice" Anticancer Research. 18. 153-158 (1998)

[文献書誌] Nagasawa,H: "Effects of ovariectomy and prolactin on mammry gland expression of transforming growth factor and epidermal growth factor receptor mRNAs in mice" Zoological Science. 15. 525-529 (1998)

[文献書誌] Denda,A: "Chemoprevention effects of nimesulide,a selective cyclooxygenease-2 inhibitor,on the development of rat urinary bladder carcinomas intiated by N-buty-N-(4-hydroxybutyl)nitrosamine." Cancer Research.58. 3028-3031 (1998)

[文献書誌] Denda.A: "Prevention by inhibitors of arachidonic acid cascade of liver carcinogenesis,cirrhosis and oxidative DNA damage caused by a choline-deficient L-amino acid-defined diet in rats." Mutation Research. 402. 279-288 (1998)

[文献書誌] Denda,A: "Inhibition by phenyl N-tert-butyl nitrone of early phase carcinogenesis in the livers of rats fed a cholinc-deficient,L-amino acid defined diet." Cancer Res.58. 4548-4551 (1998)

[文献書誌] Denda,A: "Prevention by 1'-acetoxychavicol acetate of the induction but not growth of putative preneoplastic glutathione S-transferase placental form-positive focal lesision in thelivers of rats fed a choline-deficient,L-amino acid-defined diet." Carcinogenesis. 19. 1809-1814 (1998)

[文献書誌] Denda,A: "Inhibition of early-phase exogenous and endogenous liver carcinogenesis in transgenic rats harboring a rat glutathione S-transferase placental form gene." Jpn.J Cancer Res.89. 1118-1125 (1998)

[文献書誌] Denda,A: "Induction of telomerase activity during regeration after partial hepatectomy in the rat." Cander Letter. 122. 115-120 (1998)

[文献書誌] Ohigashi,H: "Inhibitory effects of curcumin and tetrahydrocurcuminoids on the tumor promoter-induced reactive oxygen species generation in leukocytes in vitro and in vivo." Jpn.J.Cancer Res.89. 361-370 (1998)

[文献書誌] Ohigashi,H: "Superoxide scvenging activity of rosmarinic acid from Perilla frutescens Britton var acuta f vifridis" J.Agric.Food Chem.46. 4545-4550 (1998)

[文献書誌] Ohigashi,H: "Screening for in vitro anti-tumor-promoting activities of edible plants from Indonesia." Cancer Detec.Prev.22. 516-525 (1998)

[文献書誌] Ohigashi,H: "Sereening of edible Japanese plants for nitric oxide generation inhibitory activities in RAW 246.7 cells." Cancer Lett.125. 199-207 (1998)

[文献書誌] Ohigashi,H: "Niaziminin,a thiocarbamate from the leaves of Moringa oleifera,holds a strict structural requirement for inhibition of tumor-promoter-induced Epstein-Barr virus activation." Planta Med.64. 319-323 (1998)

[文献書誌] Fujiki,H: "Wide distribution of ^3H-(-)-epigallocatechin gallate,a cancer preventive tea polyphenol,in mouse tissue" Carcinogen. 19. 1771-1776 (1998)

[文献書誌] Fujiki,H: "Cancer inhibition by green tea" Mutat.Res.402. 307-310 (1998)

[文献書誌] Fujiki,H: "Molecular pathogenesis of interstitial pneumonitis with TNF-a transgenic mice" Cytokine. 10. 124-131 (1998)

[文献書誌] Sakai,T: "Promotoer activation and following induction of the p21/WAF1 gene by flavone is involved in G1 phase arrest in A549 Iung adenocarcinoma cells." FEBS Lett.437. 61-64 (1998)

[文献書誌] Sakai,T: "p53 independent activation of the gadd45 promoter by Δ^<12>-prostaglandin J_2" Biochem.Biophys.Res.Commun.251. 648-652 (1998)

[文献書誌] Sakai,T: "p53-independent induction of WAF1/Cip is correlated with osteoblastic differentiation by vitamin D3." Cancer Lett.129. 61-68 (1998)

[文献書誌] Sakai,T: "Cell cycle-dependent modulation of promoter activities of RB and WAF1/Cip1 genes." Jpn.J.Cancer Res.89. 626-633 (1998)

1998 年度実績報告書

西野輔翼京都府立医科大学, 医学部, 教授 (10079709)