2000 年度実績報告書

光合成機能統御の細胞内コミュニケーション機構

研究課題

研究課題/領域番号	09274103
研究機関	京都大学
研究代表者	佐藤文彦京都大学, 生命科学研究科, 教授 (10127087)
研究分担者	長谷俊治大阪大学, 蛋白質研究所, 教授 (00127276) 小俣達男名古屋大学, 生命農学研究科, 教授 (50175270) 杉田護名古屋大学, 人間情報学研究科, 教授 (70154474) 西村幹夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (80093061)
キーワード	葉緑体DNA結合性プロテアーゼ / 葉緑体分化と個体の生長制御 / 葉緑体RNA結合タンパク質 / PIIとNtcAによる炭素・窒素代謝制御 / プラスチドのレドックス代謝 / FdとFNRのタンパク質間相互作用 / alternative splicingの光による制御 / スプライシングのトランス因子
研究概要	1)佐藤は葉緑体DNA結合性プロテアーゼ(CND41)が老化時の緑葉においてRubiscoを特異的に分解していること、また、展開中の緑葉においてもRubiscoの分解に関わっていることを明らかとした。また、CND41は生長点における葉緑体分化を抑制し、一方、植物ホルモンであるGAの生合成と個体の生長制御にも関わっていることを明らかとした。 2)杉田は葉緑体RNA結合蛋白質(cpRNP)がストロマ中のRNAの安定性に関与していることを明らかとし、葉緑体DNAマイクロアレーを用いて、cpRNPが結合しているRNA分子種を網羅的に解明した。また、タバコのファージ型RNAポリメラーゼRpoT-Bがミトコンドリアと葉緑体の両方に移行し局在することを明らかにした。 3)小俣はラン藻のPII欠損株は、窒素を正常に利用出来るにも関わらず、GSの活性と遺伝子の発現レベルが増大していることを明らかとした。GSは制御因子NtcAによって制御されるが、この因子によって制御される他の遺伝子の発現も増加しており、PIIが何らかの機構によってNtcAの活性を制御している可能性が示唆された。 4)長谷はプラスチド内でのレドックス代謝はFdとFNRの分子種の組み合わせによる多様な電子分配機能により制御されていることを、光合成型のFd:FNRの複合体の原子レベルでの立体構造解明、C4植物の葉肉細胞と維管束鞘細胞に特異的Fdの光化学系Iからの電子分配機能が生理的に異なっていること、非光合成プラスチドのFdとFNRの特異な蛋白質間相互作用があることの観点から証明した。 5)西村は植物におけるalternative splicingには光により制御を受けているものがあることを初めて明らかにした。この光制御の解明を目指して、スプライシングのトランス因子(2種のSRタンパク質)のcDNAを単離・解析し、それらの核局在性と光照射によるmRNAの減少、消失を明らかにした。

研究成果

(30件)

すべてその他

すべて文献書誌 (30件)

[文献書誌] Murakami,S.,& Sato,F. et al.: "Protease activity of CND41, a chloroplast nucleoid DNA-binding protein, isolated from cultured tobacco cells."FEBS Lett.. 468. 15-18 (2000)
[文献書誌] Kitajima,S.,& Sato,F. et al.: "Characterization of gene expression of ERFs, transcription factors of basic PR genes from Nicotiana sylvestris."Plant Cell Physiol.. 41(1). 817-824 (2000)
[文献書誌] Sato,F. et al.: "Regulation of PR-5d (osmotin-like protein) gene expression via ERFs (ethylene responsive factors)."2000 Japan-Korea Joint Symposium. Vol.14. 103-109 (2000)
[文献書誌] Fukushima,E.& Sato,F. et al.: "Improved salt tolerance of transgenic tobacco expressing apoplastic yeast-derived invertase."Plant Cell Physiol.. 42(2). 245-249 (2001)
[文献書誌] Ifuku K.,& Sato,F.: "N-terminal sequence of the extrinsic 23 kDa protein in photosynstem II has importance in ion-retention in oxygen-evolution."Biochim.Biophys.Acta. 1546/1. 196-204 (2001)
[文献書誌] Koyama,T.& Sato,F. et al.: "Expression of PR-5d and ERF genes in cultured tobacco cells and their NaCl stress-response."Biosci.Biotech.Biochem.. 65(6)(in press).
[文献書誌] Omata,T., et al.: "Involvement of a CbbR homolog in low CO2-induced activation of the bicarbonate transporter operon in cyanobacteria."J.Bacteriol. 183(6). 1891-1898 (2001)
[文献書誌] Yonekura-Sakakibara K.,& Hase T. et al.: "Analysis of reductant supply systems for ferredoxin dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize."Plant Physiol.. 122. 887-894 (2000)
[文献書誌] Onda Y.,& Hase T. et al.: "Differential interaction of maize root ferredoxin-NADP+ oxidoreductase with photosynthetic and nonphotosynthetic ferredoxin isoproteins."Plant Physiol.. 123. 1037-1045 (2000)
[文献書誌] Kimata-Ariga,Y.,Sato,F.,& Hase,T.: "Differential electron flow around photosystem I by two C4-photosynthetic-cell-specific ferredoxins."EMBO J.. 19. 5041-5050 (2000)
[文献書誌] Nakayama,M.,& Hase,T.: "Plant sulfite reductase : molecular structure, catalytic function and interaction with ferredoxin."J.Inorg.Biochem.. 82. 27-32 (2000)
[文献書誌] Sato,N.,& Hase,T. et al.: "The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase."FEBS Lett.. 487. 247-350 (2000)
[文献書誌] 佐藤文彦: "光独立栄養培養細胞株を用いた植物機能の分子細胞生物学的解析とその応用"植物の化学調節. 35(1). 8-16 (2000)
[文献書誌] Sugita,C.,& Sugita,M. et al.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp.PCC6301 : a 33 kDa soluble polypeptide with high sequence similarity to ribosomal protein S1."Mol.Gen.Genet.. 263. 655-663 (2000)
[文献書誌] Yukawa,Y.,Sugita,M., et al.: "The TATA motif, the CAA motif and the poly (T) transcription termination motif are all important for transcription re-initiation on plant tRNA genes."The Plant J.. 22. 439-447 (2000)
[文献書誌] Nakamura,T.,& Sugita,M. et al.: "Chloroplast ribonucleoproteins function as a stabilizing factor of ribosome-free mRNAs in the stroma."J.Biol.Chem.. 276. 147-152 (2001)
[文献書誌] Hara,K.,Sugita,M., et al.: "Cloning and characterization of the cDNA for a plastid sigma factor from the moss Physcomitrella patens."Biochim.Biophys.Acta. 1517. 302-306 (2001)
[文献書誌] Maeda,S.,& Omata,T. et al.: "Bicarbonate-binding activity of the CmpA protein of the cyanobacterium Synechococcus sp.strain PCC 7942 involved in active transport of bicarbonate."J.Biol.Chem.. 275(27). 20551-20555 (2000)
[文献書誌] Kurisu,G.,& Hase,T.: "Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP+ reductase."Nature Struct.Biol.. 8. 117-121 (2001)
[文献書誌] Minami,Y.,& Nishimura,M. et al.: "Tissue and intracellular localization of indican and the purification and characterization of indican synthase from Indigo plants"Plant Cell Physiol.. 41. 218-225 (2000)
[文献書誌] Takemoto,D.,Nishimura,M. et al.: "Isolation of gene for EIR, an elicitor inducible receptor like protein, from tobacco by differential display."Plant Cell Physiol.. 41. 458-464 (2000)
[文献書誌] De Bellis,L.,& Nishimura,M. et al.: "Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase."Plant Physiol.. 123. 327-334 (2000)
[文献書誌] Mitsuhashi,N.,& Nishimura,M. et al.: "Characterization of organelles in the vacuolar-sorting pathway by -visualization with GFP in tobacco BY-2 cells."Plant Cell Physiol.. 41. 993-1001 (2000)
[文献書誌] Hayashi,M.,& Nishimura,M. et al.: "AtPex14 maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes."EMBO J.. 19. 5701-5710 (2000)
[文献書誌] Yamaguchi,K.& Nishimura,M.: "Reduction to below threshold levels of glycolate oxidase activities in transgenic tobacco enhances photoinhibition during irradiation."Plant Cell Physiol.. 41. 1397-1406 (2000)
[文献書誌] Nito,K.,& Nishimura,M. et al.: "Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures."Plant Cell Physiol.. (in press).
[文献書誌] Hayashi-Ishimaru,Y.,& Nishimura,M. et al.: "Glyoxysome-spherosome interaction in etiolated cotyledons of Arabidopsis Ped1 mutant that has defect on a thiolase gene of fatty acid b-oxidation cycle."Protoplasma. (in press).
[文献書誌] Mano,S.,& Nishimura,M. et al.: "A leaf-peroxisomal protein, hydroxypyruvate reductase, is produced by light-regulated alternative splicing."Cell Biochem.Biophys.. 32. 147-154 (2000)
[文献書誌] Kato,A.,& Nishimura,M. et al.: "Transport of peroxisomal proteins that are synthesized as large precursors in plants."Cell Biochem.Biophys.. 32. 269-275 (2000)
[文献書誌] Hayashi,M.,& Nishimura,M.: "Functional transformation of plant peroxisomes"Cell Biochem.Biophys.. 32. 295-304 (2000)

2000 年度 実績報告書

光合成機能統御の細胞内コミュニケーション機構

研究代表者

佐藤 文彦 京都大学, 生命科学研究科, 教授 (10127087)

研究成果

[文献書誌] Murakami,S.,& Sato,F. et al.: "Protease activity of CND41, a chloroplast nucleoid DNA-binding protein, isolated from cultured tobacco cells."FEBS Lett.. 468. 15-18 (2000)

[文献書誌] Kitajima,S.,& Sato,F. et al.: "Characterization of gene expression of ERFs, transcription factors of basic PR genes from Nicotiana sylvestris."Plant Cell Physiol.. 41(1). 817-824 (2000)

[文献書誌] Sato,F. et al.: "Regulation of PR-5d (osmotin-like protein) gene expression via ERFs (ethylene responsive factors)."2000 Japan-Korea Joint Symposium. Vol.14. 103-109 (2000)

[文献書誌] Fukushima,E.& Sato,F. et al.: "Improved salt tolerance of transgenic tobacco expressing apoplastic yeast-derived invertase."Plant Cell Physiol.. 42(2). 245-249 (2001)

[文献書誌] Ifuku K.,& Sato,F.: "N-terminal sequence of the extrinsic 23 kDa protein in photosynstem II has importance in ion-retention in oxygen-evolution."Biochim.Biophys.Acta. 1546/1. 196-204 (2001)

[文献書誌] Koyama,T.& Sato,F. et al.: "Expression of PR-5d and ERF genes in cultured tobacco cells and their NaCl stress-response."Biosci.Biotech.Biochem.. 65(6)(in press).

[文献書誌] Omata,T., et al.: "Involvement of a CbbR homolog in low CO2-induced activation of the bicarbonate transporter operon in cyanobacteria."J.Bacteriol. 183(6). 1891-1898 (2001)

[文献書誌] Yonekura-Sakakibara K.,& Hase T. et al.: "Analysis of reductant supply systems for ferredoxin dependent sulfite reductase in photosynthetic and nonphotosynthetic organs of maize."Plant Physiol.. 122. 887-894 (2000)

[文献書誌] Onda Y.,& Hase T. et al.: "Differential interaction of maize root ferredoxin-NADP+ oxidoreductase with photosynthetic and nonphotosynthetic ferredoxin isoproteins."Plant Physiol.. 123. 1037-1045 (2000)

[文献書誌] Kimata-Ariga,Y.,Sato,F.,& Hase,T.: "Differential electron flow around photosystem I by two C4-photosynthetic-cell-specific ferredoxins."EMBO J.. 19. 5041-5050 (2000)

[文献書誌] Nakayama,M.,& Hase,T.: "Plant sulfite reductase : molecular structure, catalytic function and interaction with ferredoxin."J.Inorg.Biochem.. 82. 27-32 (2000)

[文献書誌] Sato,N.,& Hase,T. et al.: "The 70-kDa major DNA-compacting protein of the chloroplast nucleoid is sulfite reductase."FEBS Lett.. 487. 247-350 (2000)

[文献書誌] 佐藤文彦: "光独立栄養培養細胞株を用いた植物機能の分子細胞生物学的解析とその応用"植物の化学調節. 35(1). 8-16 (2000)

[文献書誌] Sugita,C.,& Sugita,M. et al.: "A novel nucleic acid-binding protein in the cyanobacterium Synechococcus sp.PCC6301 : a 33 kDa soluble polypeptide with high sequence similarity to ribosomal protein S1."Mol.Gen.Genet.. 263. 655-663 (2000)

[文献書誌] Yukawa,Y.,Sugita,M., et al.: "The TATA motif, the CAA motif and the poly (T) transcription termination motif are all important for transcription re-initiation on plant tRNA genes."The Plant J.. 22. 439-447 (2000)

[文献書誌] Nakamura,T.,& Sugita,M. et al.: "Chloroplast ribonucleoproteins function as a stabilizing factor of ribosome-free mRNAs in the stroma."J.Biol.Chem.. 276. 147-152 (2001)

[文献書誌] Hara,K.,Sugita,M., et al.: "Cloning and characterization of the cDNA for a plastid sigma factor from the moss Physcomitrella patens."Biochim.Biophys.Acta. 1517. 302-306 (2001)

[文献書誌] Maeda,S.,& Omata,T. et al.: "Bicarbonate-binding activity of the CmpA protein of the cyanobacterium Synechococcus sp.strain PCC 7942 involved in active transport of bicarbonate."J.Biol.Chem.. 275(27). 20551-20555 (2000)

[文献書誌] Kurisu,G.,& Hase,T.: "Structure of the electron transfer complex between ferredoxin and ferredoxin-NADP+ reductase."Nature Struct.Biol.. 8. 117-121 (2001)

[文献書誌] Minami,Y.,& Nishimura,M. et al.: "Tissue and intracellular localization of indican and the purification and characterization of indican synthase from Indigo plants"Plant Cell Physiol.. 41. 218-225 (2000)

[文献書誌] Takemoto,D.,Nishimura,M. et al.: "Isolation of gene for EIR, an elicitor inducible receptor like protein, from tobacco by differential display."Plant Cell Physiol.. 41. 458-464 (2000)

[文献書誌] De Bellis,L.,& Nishimura,M. et al.: "Purification and characterization of a novel pumpkin short-chain acyl-CoA oxidase which structure resembles acyl-CoA dehydrogenase."Plant Physiol.. 123. 327-334 (2000)

[文献書誌] Mitsuhashi,N.,& Nishimura,M. et al.: "Characterization of organelles in the vacuolar-sorting pathway by -visualization with GFP in tobacco BY-2 cells."Plant Cell Physiol.. 41. 993-1001 (2000)

[文献書誌] Hayashi,M.,& Nishimura,M. et al.: "AtPex14 maintains peroxisomal functions by determining protein targeting to three kinds of plant peroxisomes."EMBO J.. 19. 5701-5710 (2000)

[文献書誌] Yamaguchi,K.& Nishimura,M.: "Reduction to below threshold levels of glycolate oxidase activities in transgenic tobacco enhances photoinhibition during irradiation."Plant Cell Physiol.. 41. 1397-1406 (2000)

[文献書誌] Nito,K.,& Nishimura,M. et al.: "Pumpkin peroxisomal ascorbate peroxidase is localized on peroxisomal membranes and unknown membranous structures."Plant Cell Physiol.. (in press).

[文献書誌] Hayashi-Ishimaru,Y.,& Nishimura,M. et al.: "Glyoxysome-spherosome interaction in etiolated cotyledons of Arabidopsis Ped1 mutant that has defect on a thiolase gene of fatty acid b-oxidation cycle."Protoplasma. (in press).

[文献書誌] Mano,S.,& Nishimura,M. et al.: "A leaf-peroxisomal protein, hydroxypyruvate reductase, is produced by light-regulated alternative splicing."Cell Biochem.Biophys.. 32. 147-154 (2000)

[文献書誌] Kato,A.,& Nishimura,M. et al.: "Transport of peroxisomal proteins that are synthesized as large precursors in plants."Cell Biochem.Biophys.. 32. 269-275 (2000)

[文献書誌] Hayashi,M.,& Nishimura,M.: "Functional transformation of plant peroxisomes"Cell Biochem.Biophys.. 32. 295-304 (2000)

2000 年度実績報告書

佐藤文彦京都大学, 生命科学研究科, 教授 (10127087)