1999 年度実績報告書

歯髄・歯周組織の老化機構の研究

研究課題

研究課題/領域番号	09307042
研究機関	東京医科歯科大学
研究代表者	須田英明東京医科歯科大学, 歯学部, 教授 (00114760)
研究分担者	永田俊彦徳島大学, 歯学部, 教授 (10127847) 石川烈東京医科歯科大学, 歯学部, 教授 (10014151) 前田勝正九州大学, 歯学部, 教授 (00117243) 西村英紀岡山大学, 歯学部, 講師 (80208222) 永井淳福岡歯科大学, 歯学部, 助教授 (70252989)
キーワード	歯髄 / 歯周組織 / 老化機構
研究概要	1.若年ラットではIL-1αがマクロファージで発現し,病巣が緩やかに拡大した. 2.根管の感染による根尖性歯周炎の治癒は,加齢に伴い遅延する傾向があった. 3.ラット歯髄では,窩洞形成に対する抗原提示細胞の反応は加齢により量的な減弱傾向を示したが,組織球や血管由来の単球による生体防御反応は加齢により減弱しなかった. 4.若年ラットの歯肉組織中で働く分解系抑制が歯肉増殖の一因になっていた. 5.歯髄の侵害性刺激により誘発される反射性咀嚼筋活動には加齢的変化が生じていた.また,ヒト歯髄中のアルカリフォスファターゼ活性は加齢に伴い減少していた. 6.歯根膜由来の培養細胞では,ドナーの加齢に伴い,SPARCの産生が減少していた. 7.個体の加齢に伴い,歯根膜細胞の分裂寿命が低下し,遊走能も低下していた.また,老化細胞における走化活性の低下にc-fosの発現低下が深く関与していた. 8.歯髄および歯肉血流の測定法を確立し,その血流値には加齢に伴う変化が生じていた. 9.慢性歯周炎におけるIL-17産生は,加齢による変化よりもむしろ個体の持つ免疫学的な背景に大きく影響されていた. 10.有歯顎者の腰椎および下顎骨皮質骨骨密度は無歯顎者に比べて高く,特に有歯顎者においては下顎骨骨代謝が歯の存在に伴う局所因子に強く影響されていた.

研究成果
(18件)

すべてその他

すべて文献書誌 (18件)

[文献書誌] Matsui Y: "Age-Related Changes in the Reflex-Evoked Jaw Muscle Activities by Tooth Pulp Noxious Chemical Stimulation"Japanese Journal of Oral Brology. (in press). (2000)
[文献書誌] Ikawa M: "The effects of thermal and mechanical stimulation on blood flow in healthy and inflamed gingiva in man."Archs of Oral Brol. 43. 127-132 (1998)
[文献書誌] Ikawa M: "Scattering of laser light directed onto the labial surface of extracted human upper central incisors."J Endocl. 25. 483-485 (1999)
[文献書誌] Bando K: "Bone mineral density in periodontally heaithy and edentulous postmenopausal women."Annals of Periodontology. 3. 321-326 (1998)
[文献書誌] Nishimura F: "Periodontal disease as complication of diabetes mellitus."Annals of Periodontology. 3. 20-29 (1998)
[文献書誌] Ohyama H: "T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis."Hum Immunol. 59. 635-643 (1998)
[文献書誌] Naruishi K: "Role of soluble interleukin-6 receptor in inflamed gingiva for binding of interleukin-6 to gingival fibroblasts."J Periodontal Res. 34. 296-300 (1999)
[文献書誌] Sawada K: "Identification of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis by subtractive hybridization."Infect Immun. 67. 5621-5625 (1999)
[文献書誌] Yamada H: "Phenytoin and cyclosporin-A supress the expression of MMP-1,TIMP-1 and cathepsin L, but not that of cathepson-B in cultured gingival fibroblasts."J Periodontol. in press. (2000)
[文献書誌] Sakata M: "Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells."J Bone Miner Res.. 14. 1486-1492 (1999)
[文献書誌] Kataoka M: "Cyclosporin A decreases the degradation of Type I collagen in rat gingival overgrowth."J Cellular Physrology. 182(in press). (2000)
[文献書誌] Nagai A: "Interleukin-17 production in adult periodontitis."J Dent Res.. 78. 184 (1999)
[文献書誌] Ito H: "Haptotactic migration of pancreatic cancer cells induced by bioactive components in bovine liver extract."J Surg Oncol.. 68. 153-158 (1998)
[文献書誌] Sawa T: "In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesion by exogeneous Fas-ligand."Infect Immun. 67. 1450-1454 (1999)
[文献書誌] Katsuragi K: "Immunopathological findings of an early-onset periodontitis patient with decreased CD18 expression."J Int Acad Periodontol. 1. 35-44 (1999)
[文献書誌] Asahara Y: "Chemotactic response of periodontal ligament cells decreases with donor age: association with reduced expression of c-fos."Oral Diseases. 5. 337-343 (1999)
[文献書誌] Nishimura F: "Migration inhibitory factor related protein-8 (MRP-8) is an autocrine chemotactic factor for human periodontal ligament cells."J Dent Res.. 78. 1251-1255 (1999)
[文献書誌] Sawada S: "Phylogenetic characterization of Centipeda Periodontii, Selenomonas sputigena Selenomonas species by 16S rRNA gene sequence analysis."Microbios. 98. 133-140 (1999)

1999 年度 実績報告書

歯髄・歯周組織の老化機構の研究

研究代表者

須田 英明 東京医科歯科大学, 歯学部, 教授 (00114760)

研究成果

[文献書誌] Matsui Y: "Age-Related Changes in the Reflex-Evoked Jaw Muscle Activities by Tooth Pulp Noxious Chemical Stimulation"Japanese Journal of Oral Brology. (in press). (2000)

[文献書誌] Ikawa M: "The effects of thermal and mechanical stimulation on blood flow in healthy and inflamed gingiva in man."Archs of Oral Brol. 43. 127-132 (1998)

[文献書誌] Ikawa M: "Scattering of laser light directed onto the labial surface of extracted human upper central incisors."J Endocl. 25. 483-485 (1999)

[文献書誌] Bando K: "Bone mineral density in periodontally heaithy and edentulous postmenopausal women."Annals of Periodontology. 3. 321-326 (1998)

[文献書誌] Nishimura F: "Periodontal disease as complication of diabetes mellitus."Annals of Periodontology. 3. 20-29 (1998)

[文献書誌] Ohyama H: "T cell responses to 53-kDa outer membrane protein of Porphyromonas gingivalis in humans with early-onset periodontitis."Hum Immunol. 59. 635-643 (1998)

[文献書誌] Naruishi K: "Role of soluble interleukin-6 receptor in inflamed gingiva for binding of interleukin-6 to gingival fibroblasts."J Periodontal Res. 34. 296-300 (1999)

[文献書誌] Sawada K: "Identification of a novel insertion sequence specific for virulent strains of Porphyromonas gingivalis by subtractive hybridization."Infect Immun. 67. 5621-5625 (1999)

[文献書誌] Yamada H: "Phenytoin and cyclosporin-A supress the expression of MMP-1,TIMP-1 and cathepsin L, but not that of cathepson-B in cultured gingival fibroblasts."J Periodontol. in press. (2000)

[文献書誌] Sakata M: "Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells."J Bone Miner Res.. 14. 1486-1492 (1999)

[文献書誌] Kataoka M: "Cyclosporin A decreases the degradation of Type I collagen in rat gingival overgrowth."J Cellular Physrology. 182(in press). (2000)

[文献書誌] Nagai A: "Interleukin-17 production in adult periodontitis."J Dent Res.. 78. 184 (1999)

[文献書誌] Ito H: "Haptotactic migration of pancreatic cancer cells induced by bioactive components in bovine liver extract."J Surg Oncol.. 68. 153-158 (1998)

[文献書誌] Sawa T: "In vitro induction of activation-induced cell death in lymphocytes from chronic periodontal lesion by exogeneous Fas-ligand."Infect Immun. 67. 1450-1454 (1999)

[文献書誌] Katsuragi K: "Immunopathological findings of an early-onset periodontitis patient with decreased CD18 expression."J Int Acad Periodontol. 1. 35-44 (1999)

[文献書誌] Asahara Y: "Chemotactic response of periodontal ligament cells decreases with donor age: association with reduced expression of c-fos."Oral Diseases. 5. 337-343 (1999)

[文献書誌] Nishimura F: "Migration inhibitory factor related protein-8 (MRP-8) is an autocrine chemotactic factor for human periodontal ligament cells."J Dent Res.. 78. 1251-1255 (1999)

[文献書誌] Sawada S: "Phylogenetic characterization of Centipeda Periodontii, Selenomonas sputigena Selenomonas species by 16S rRNA gene sequence analysis."Microbios. 98. 133-140 (1999)

1999 年度実績報告書

須田英明東京医科歯科大学, 歯学部, 教授 (00114760)