1998 年度実績報告書

IP_3受容体の細胞内Ca^<2+>制御機構と情報伝達及び発生・分化と可塑性における役割

研究課題

研究課題/領域番号	09308030
研究機関	東京大学
研究代表者	御子柴克彦東京大学, 医科学研究所, 教授 (30051840)
研究分担者	道川貴章東京大学, 医科学研究所, 助手 (90282516) 古市貞一東京大学, 医科学研究所, 助教授 (50219094)
キーワード	IP3受容体 / Ca2+動態 / 神経突起伸長 / 成長円錐 / 神経伝達物質 / LTP / 胚軸形成 / LTD
研究概要	(1) 神経突起伸長と成長円錐の形態変化を、各部位でIP3受容体、各種骨格蛋白質、カルモジュリン関連蛋白質をレーザー光により欠落させた際の変化の解析を行った。 (2) IP3受容体の単一イオンチャネル解析。形質発現させた受容体蛋白質を、特異抗体をカップルさせたアフィニティ・カラムを用いて精製した。 (3) 蛍光測光・画像処理システムを用いた細胞内Ca2+動態を解析。タイプ別に発現する細胞種を用いて、ホルモン・神経伝達物質・成長因子などの刺激を与えたときのCa2+蛍光指示薬を用いたCa2+動態解析をした。 (4) IP3受容体をノックアウトしたマウスの各種細胞生物学的解析を行う。 (5) IP3受容体、PI代謝回転の阻害の胚軸形成に対する効果の検討;以下に述べるIP3受容体、PI-代謝回転、Protein Kinase C(PKC)のブロッカーを用いて、初期胚背側あるいは腹側の細胞に注入し、発生に対する影響を検討中である。すでにIP3レセプター抗体はアフリカツメガエルの受精、卵の活性化やその他のIP3依存性の現象を抑制することを明らかにしている。各々のブロッカーの作用を検討することにより、胚軸形成における、PI-シグナル系のIP3カスケードとPKCカスケードの各々の役割を、より明確にできる。

研究成果

(23件)

すべてその他

すべて文献書誌 (23件)

[文献書誌] Aruga, J.et.al.: "Mouse Zic1 is involved in cerebeller development." J.Neurosci.18. 284-293 (1998)
[文献書誌] Monkawa, T.et.al.: "Localization of inositol 1,4,5-trisphophate receptors in the rat kidney." Kidney lnt.53. 296-301 (1998)
[文献書誌] Zhao, H.et.al.: "Functional expression of a mammalian odor ant receptor." Science. 279. 237-242 (1998)
[文献書誌] Sato, Y.et.al.: "Adenophostin, a potent agonist of the inositol 1,4,5-trisphosphate receptor, is useful for fertilization of mouse oocytes injected with round spermatids leading to normal offspring" Biology of Reproduction. 58. 867-873 (1998)
[文献書誌] Miyamura, T.et.al.: "Metabolic labeling of a subset of glia cells by UDP-galactose : implication for astrocyte lineage diversity." J.Neurosci. Res.52. 173-183 (1998)
[文献書誌] Yamamoto-Hino, M.: "Apical vesicles bearing inositol 1,4,5-trisphospate receptors in the Ca2+ initiation site of ductal epithelium of submandibular gland." J.Cell.Biol.141. 135-142 (1998)
[文献書誌] Ibata, K.et.al.: "Inositol 1,3,4,5-Tetrakisphosphate binding activities of neural and non-neural synaptotagmins." J.Biol.Chem.273. 12267-12273 (1998)
[文献書誌] Inoue, T.et.al.: "Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar purkinje neurons" J.Neurosci.18. 5366-5373 (1998)
[文献書誌] Mathias, R.S.et.al.: "IP3 receptor blockade fails to prevent intracellular Ca2+ release by ET-1 and a-thrombin." Am.J.Physiol.274. C1456-C1465 (1998)
[文献書誌] Hirota, J.et.al.: "T-cell receptor signalling in the inositol 1,4,5-trisphosphate receptor (IP3R) type-1-deficient mice : is IP3R type 1 essential for T-cell-receptor signalling?" Biochem. J.333. 615-619 (1998)
[文献書誌] Fukuzono, S.et.al.: "Overproduction and immuno-affinity purification of myelin proteolipid protein (PLP), aninositol hexakisphosphate binding protein, in a bacul ovirus expression system." Biochem.Biophys.Res.Commun.249. 66-72 (1998)
[文献書誌] Muto, A.et.al.: "Activation of inositol 1,4,5-trisphosphate receptors induces transient changes in cell shape of fertilized Xenopus eggs." Cell Motility and the Cytoskeleton. 39. 201-208 (1998)
[文献書誌] Nakata, K.et.al.: "Xenopus Zic family and its role in neural and neural crest development." Mechanisms of Development. 75. 43-51 (1998)
[文献書誌] Nakayama, T.et.al.: "Hemizygous deletion of the HPC-1/syntaxin 1A gene (STX1A) in patient with Williams syndrome." Cytogenet Cell Genet.82. 49-51 (1998)
[文献書誌] Yoshida, M.et.al.: "Role of two series of Ca2+ oscillations in activation of ascidian eggs." Developmenatal Biology. 203. 122-133 (1998)
[文献書誌] Futatsugi, A.et.al.: "Muscle-specific mRNA isoform encodes a protein composed mainly of the N-terminal 175 residues of type 2 ins (1,4,5) P3 receptor." Biochem.J.334. 559-563 (1998)
[文献書誌] Hata, R.et.al.: "Brainstem auditory evoked potentials during brainstem ischemia and reperfusion in gerbils." Neuroscience. 83. 201-213 (1998)
[文献書誌] Takei, K.et.al.: "Regulation of nerve growth mediated by inositol 1,4,5-trisphosphate receptor in growth cones." Science. 282. 1705-1708 (1998)
[文献書誌] Tamura, H.et.al.: "Molecular cloning and expression of cDNA encoding an olfactory-specific mouse phenol sulphotransferase." Biochem.J.331. 953-958 (1998)
[文献書誌] Yoshikawa, F.et.al.: "Cooperative for mation of the ligand-binding site of the inositol 1,4,5-trisphosphate receptor by two separable domains." J.Biol.Chem.274. 328-334 (1999)
[文献書誌] Yoshikawa, F.et.al.: "Trypsinized cer ebellar inositol 1,4,5-trisphosphate receptor." J.Biol.Chem.274. 316-327 (1999)
[文献書誌] Kabayama, H.et.al.: "Functional involvement of synaptotagmin I/II C2A domain in neurite outgrowth of chick dorsal root ganglion neuron." Neuroscience. 88. 999-1003 (1999)
[文献書誌] Zhu, C.et.al.: "Inosiotl 1,4,5-trisphosphate receptor down-regulation is activated directly by inositol 1,4,5-trisphosphate binding." J.Biol.Chem.274. 3476-3484 (1999)

1998 年度 実績報告書

IP_3受容体の細胞内Ca^<2+>制御機構と情報伝達及び発生・分化と可塑性における役割

研究代表者

御子柴 克彦 東京大学, 医科学研究所, 教授 (30051840)

研究成果

[文献書誌] Aruga, J.et.al.: "Mouse Zic1 is involved in cerebeller development." J.Neurosci.18. 284-293 (1998)

[文献書誌] Monkawa, T.et.al.: "Localization of inositol 1,4,5-trisphophate receptors in the rat kidney." Kidney lnt.53. 296-301 (1998)

[文献書誌] Zhao, H.et.al.: "Functional expression of a mammalian odor ant receptor." Science. 279. 237-242 (1998)

[文献書誌] Sato, Y.et.al.: "Adenophostin, a potent agonist of the inositol 1,4,5-trisphosphate receptor, is useful for fertilization of mouse oocytes injected with round spermatids leading to normal offspring" Biology of Reproduction. 58. 867-873 (1998)

[文献書誌] Miyamura, T.et.al.: "Metabolic labeling of a subset of glia cells by UDP-galactose : implication for astrocyte lineage diversity." J.Neurosci. Res.52. 173-183 (1998)

[文献書誌] Yamamoto-Hino, M.: "Apical vesicles bearing inositol 1,4,5-trisphospate receptors in the Ca2+ initiation site of ductal epithelium of submandibular gland." J.Cell.Biol.141. 135-142 (1998)

[文献書誌] Ibata, K.et.al.: "Inositol 1,3,4,5-Tetrakisphosphate binding activities of neural and non-neural synaptotagmins." J.Biol.Chem.273. 12267-12273 (1998)

[文献書誌] Inoue, T.et.al.: "Type 1 inositol 1,4,5-trisphosphate receptor is required for induction of long-term depression in cerebellar purkinje neurons" J.Neurosci.18. 5366-5373 (1998)

[文献書誌] Mathias, R.S.et.al.: "IP3 receptor blockade fails to prevent intracellular Ca2+ release by ET-1 and a-thrombin." Am.J.Physiol.274. C1456-C1465 (1998)

[文献書誌] Hirota, J.et.al.: "T-cell receptor signalling in the inositol 1,4,5-trisphosphate receptor (IP3R) type-1-deficient mice : is IP3R type 1 essential for T-cell-receptor signalling?" Biochem. J.333. 615-619 (1998)

[文献書誌] Fukuzono, S.et.al.: "Overproduction and immuno-affinity purification of myelin proteolipid protein (PLP), aninositol hexakisphosphate binding protein, in a bacul ovirus expression system." Biochem.Biophys.Res.Commun.249. 66-72 (1998)

[文献書誌] Muto, A.et.al.: "Activation of inositol 1,4,5-trisphosphate receptors induces transient changes in cell shape of fertilized Xenopus eggs." Cell Motility and the Cytoskeleton. 39. 201-208 (1998)

[文献書誌] Nakata, K.et.al.: "Xenopus Zic family and its role in neural and neural crest development." Mechanisms of Development. 75. 43-51 (1998)

[文献書誌] Nakayama, T.et.al.: "Hemizygous deletion of the HPC-1/syntaxin 1A gene (STX1A) in patient with Williams syndrome." Cytogenet Cell Genet.82. 49-51 (1998)

[文献書誌] Yoshida, M.et.al.: "Role of two series of Ca2+ oscillations in activation of ascidian eggs." Developmenatal Biology. 203. 122-133 (1998)

[文献書誌] Futatsugi, A.et.al.: "Muscle-specific mRNA isoform encodes a protein composed mainly of the N-terminal 175 residues of type 2 ins (1,4,5) P3 receptor." Biochem.J.334. 559-563 (1998)

[文献書誌] Hata, R.et.al.: "Brainstem auditory evoked potentials during brainstem ischemia and reperfusion in gerbils." Neuroscience. 83. 201-213 (1998)

[文献書誌] Takei, K.et.al.: "Regulation of nerve growth mediated by inositol 1,4,5-trisphosphate receptor in growth cones." Science. 282. 1705-1708 (1998)

[文献書誌] Tamura, H.et.al.: "Molecular cloning and expression of cDNA encoding an olfactory-specific mouse phenol sulphotransferase." Biochem.J.331. 953-958 (1998)

[文献書誌] Yoshikawa, F.et.al.: "Cooperative for mation of the ligand-binding site of the inositol 1,4,5-trisphosphate receptor by two separable domains." J.Biol.Chem.274. 328-334 (1999)

[文献書誌] Yoshikawa, F.et.al.: "Trypsinized cer ebellar inositol 1,4,5-trisphosphate receptor." J.Biol.Chem.274. 316-327 (1999)

[文献書誌] Kabayama, H.et.al.: "Functional involvement of synaptotagmin I/II C2A domain in neurite outgrowth of chick dorsal root ganglion neuron." Neuroscience. 88. 999-1003 (1999)

[文献書誌] Zhu, C.et.al.: "Inosiotl 1,4,5-trisphosphate receptor down-regulation is activated directly by inositol 1,4,5-trisphosphate binding." J.Biol.Chem.274. 3476-3484 (1999)

1998 年度実績報告書

御子柴克彦東京大学, 医科学研究所, 教授 (30051840)