1997 年度実績報告書

発生・形態形成の個体レベルにおける分子生物学的研究

研究課題

研究課題/領域番号	09NP0401
研究機関	京都大学
研究代表者	竹市雅俊京都大学, 大学院・理学研究科, 教授 (00025454)
研究分担者	高田慎治京都大学, 理学部, 助教授 (60206753) 能瀬聡直岡崎国立共同研究機構, 基礎生物学研究所, 助手 (30260037) 近藤寿人大阪大学, 細胞生体工学センター, 教授 (70127083) 安田國雄国立奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (30025473) 岡野栄之大阪大学, 医学部, 教授 (60160694)
キーワード	発生 / 遺伝子ターゲッティング / 形態形成 / 細胞分化 / 細胞認識 / 神経発生 / 遺伝子トラッピング / ES細胞
研究概要	1.神経系カドヘリンの機能解析を行い、カドヘリン11は海馬のLTPの抑制に関与し、ショウジョウバエDN-カドヘリンは、成虫脳全体のオ-ガニゼーションのために必須であること、などを明らかにした。また、Capriciousについて機能欠失株における運動神経投射パターンなどを解析し、シナプス形成過程における役割を明らかにした。 2.L-Mafの強制発現により外胚葉が水晶体に分化することなどをみつけ、水晶体分化のマスター遺伝子であることを示した。また、頭部側方外胚葉では、眼胞によってSox2/Sox3の発現が誘導され、この領域が水晶体プラコードとなることがわかった。肢芽における異所的なHox発現が、筋肉塊の増殖や順次断裂によるパターン形成を阻害することを示した。Notchの細胞内シグナル機構を探り、Notch→Hes1/Hes5経路の存在を明らかにした。Islet-2機能を阻害した胚では、この遺伝子を発現する感覚神経細胞と運動神経細胞の軸索伸展に特異的な異常が見られた。マウスエピブラストを、上側と含まない下側に切り分けて初代培養すると、上半分の断片からのみ始原生殖細胞が分化することを明らかにした。Wnt-1/Wnt-3a両遺伝子は神経冠細胞の形成の他に、皮筋節の内側部の形成にも必須であることがわかった。 jmj変異体の表現型を解析し、血球系幹細胞、心筋細胞の発生に深く関わることを示した。胚様体形成をスクリーニング系として、loxPをトラップベクターに組込んだシステムで遺伝子トラップを行なった結果、この方法が極めて効率のよいことが判明した。mouse-musashi-1遺伝子のノックアウトでは、胎生後期に約50%が致死となり、残りの50%は頭部の形態異常やグリア系の細胞の分化異常などの表現型を示した。

研究成果
(76件)

すべてその他

すべて文献書誌 (76件)

[文献書誌] Oda,H.,Uemura,T.,Takeichi,M.: "Phenotypic analysis of null mutants for DE-cadherin and Armadillo in Drosophila ovaries reveals distinct aspects of their functions in cell adhesion and cytoskeletal organization." Genes to Cells. 2. 29-40
[文献書誌] Inoue,T.,Chisaka,O.,Takeichi,M.et al.: "Cadherin-6 expression transiently delineates specific rhombomeres,other neural tube subdivisions and neural crest subpopulations in mouse embryos." Develop.Biol.183. 183-194
[文献書誌] Fushimi,D.,Arndt,K.,Takeichi,M.et al.: "Cloning and expression analysis of cadherin-10 in the CNS.of the chicken embryos." Develop.Dynamics. 209. 1-17
[文献書誌] Nose,A.,Umeda,T.,Takeichi,M.: "Neuromuscular target recognition by a homophilc inteaction of Connectin cell adhesion molecules in Drosophila." Development. 124. 1433-1441
[文献書誌] Radice,G.L.,Rayburn,H.,Takeichi,M.et al.: "Developmental defects in mouse embryos lacking N-cadherin." Develop.Biol.181. 64-78
[文献書誌] Umemiya,T.,Takeichi,M.,Nose,A.: "M-spondin,a novel ECM protein highly homologous to vertebrate F-spondin,is localized at the muscle attachment sites in the Drosophila embryos." Develop.Biol.186. 165-176
[文献書誌] Nakagawa,S.,Takeichi,M.: "N-cadherin is crucial for hear formation in the chick embryo." Develop.Growth Differ.39. 451-455
[文献書誌] Iwai,Y.,Usui,T.,Takeichi,M.et al.: "Axon patterning requires DN-cadherin,a novel neuronal adhesion receptor,in the Drosophila embryonic CNS." Neuron. 19. 77-89
[文献書誌] Isshiki,T.,Takeichi,M.,Nose,A.: "The role of the msh homeobox gene during neurogenesis:implication for the dorsoventral specification of the neuroectoderm." Development. 124. 3099-3109
[文献書誌] Suzuki,S.C.,Inoue,T.,Takeichi,M.et al.: "Neuronal circuits are subdivided by differential expression of type-II classic cadherins in postnatal mouse brains." Mol.Cell.Neuroscience. 9. 433-447
[文献書誌] Nose,A.,Isshiki,T.,Takeichi,M.: "Regional specification of muscle progenitors in Drosophila:the role of the msh homeobox gene." Development. 125. 215-223
[文献書誌] Inoue,T.,Tanaka,T.,Takeichi,M.et al.: "Cadherin-6 in the developing mouse brain:Expression along restricted connection systems and synaptic localization suggest a potential role in neuronal circuitry." Develop.Dynamics. (in press).
[文献書誌] Arndt,K.,Nakagawa,S.,Takeichi,M.et al.: "Cadherin-defined segment and parasagittal cell ribbons in the developing chicken cerebellum." Mol.Cell.Neuroscience. (in press).
[文献書誌] 能瀬聡直、梅田達夫、竹市雅俊: "Neuromuscular target recognition by a homophilic interaction of Connectin cell adhesion molecules in Drosophila." Development. 124. 1433-1441 (1997)
[文献書誌] 梅宮猛、竹市雅俊、能瀬聡直: "M-spondin,a novel ECM Protein highly homologous to vertebrate F-spondin is localized at the muscle attachment sites in the Drosophila embryo." Developmental Biology. 186. 165-175 (1997)
[文献書誌] 一色孝子、竹市雅俊、能瀬聡直: "The role of the msh homeobox gene during Drosophila neurogenesis;implication for the dorsoventral specification of the CNS." Development. 124. 3099-3109 (1997)
[文献書誌] 能瀬聡直、一色孝子、竹市雅俊: "Regional specification of muscle progenitors in Drosophila:the role of the msh homeobox gene." Development. 125. 215-223 (1998)
[文献書誌] 岡本仁: "脳の複雑化と多様化" 現代化学. 11月号. 53-59 (1997)
[文献書誌] Kikuchi,Y.,Segawa,H.,Tokumoto,M.,Ttsubokawa,T.,Hotta,Y.,Uyemura,K.,and Okamoto,H.: "Ocular and cerebellar defects in zebrafish induced by overexpression of the LIM domain of the Islet-3 LIM/homeodomain protein." Neuron. 18. 369-382 (1997)
[文献書誌] Higashijima,S.,Nose,A.,Eguchi,G.,Hotta,Y.,and Okamoto,H.: "Mindin/F-spondin family:novel ECM proteins expressed in the zebrafish embryonic axis." Developmental Biology. 192. 211-277 (1997)
[文献書誌] Higashijima,S.,Okamoto,H.,Ueno,N.,Hotta,Y.,and Eguchi G.: "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using prooter of zebrafish origin." Developmental Biology. 192. 289-299 (1998)
[文献書誌] Hajime Ogino,Kunio Yasuda: "Induction of lens differntiation by activation of a bZIP transcription factor L-Maf" Science. (in press).
[文献書誌] Takuya Murata,Masahiro Nitta,Kunio Yasuda: "Involvement of the CP2 transcription factor family in lens-specific expression of the chicken A-crystallin gene." (submitted).
[文献書誌] Masahi Kitamura,Kumi Miyauchi,Kunio Yasuda: "Purification and immunological analyses of a nuclear factor,CEF1,that binds to a lens-specific enhnacer of the chicken A-crystallin" (submitted).
[文献書誌] Takebayashi,K.,Takahashi,S.,Yokota,C.,Tsuda,H.,Nakanishi,S.,Asashima,M.,and Kageyama,R.: "Conversion of ectoderm into a neural fate by ATH-3,a vertebrate basic helix-loop-helix gene homologous to Drosophila proneural gene atonal." EMBO J.16. 384-395 (1997)
[文献書誌] Matsue,M.,Kageyama,R.,Denhardt,D.T.,and Noda,M.: "Helix-loop-helix-type transcription factor(HES-1)is expressed in osteoblastic cells,suppressed by 1,25(OH)2 vitamin D3,and modulates 1,25(OH)2 vitamin D3 enhancement of osteopontin gene expression." Bone. 20. 329-334 (1997)
[文献書誌] Kageyama,R.and Nakanishi,S.: "Helix-loop-helix factors in growth and differentiation of the nervous system." Curr.Op.Genet.Dev.7. 659-665 (1997)
[文献書誌] Kageyama,R.,Ishibashi,M.,Takebayashi,K.,and Tomita,K.: "bHLH transcription factors and mammalian neuronal differentiation." Int.J.Biochem.Cell Biol.(in press). (1998)
[文献書誌] Ikeya,M.,Lee,S.M.K.,Johnson,J.E.,McMahon,A.P.& Takada,s.: "Wnt signalling required for expansion of neural crest and CNS progenitors." Nature. 389. 966-970 (1997)
[文献書誌] Yoshikawa,Y.,Fujimori,T.,McMahon,A.P.& Takada,S.: "Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse." Dev.Biol.183. 234-242 (1997)
[文献書誌] Nagai,T.,Aruga,J.,Takada,S.,Gunther,T.,Sporle,R.,Schughart,K.& Mikoshiba,K.: "The expression of the mouse Zic1,Zic2,and Zic3 gene suggests an essential role for Zic genes in body pattern formation." Dev.Biol.182. 299-313 (1997)
[文献書誌] Tsuda,H.,Takebayashi,K.,Nakanishi,S.,and Kageyama,R.: "Structure and promoter analysis of Math3 gene,a mouse homolog of Drosophila proneural gene atonal:neural-specific expression by dual promoter elements." J.Biol.Chem.(in press.). (1998)
[文献書誌] Nishimura,M.,Isaka,F.,Ishibashi,M.,Tomita,K.,Tsuda,H.,Nakanishi,S.,and Kageyama,R.: "Structure,chromosomal locus and promoter of mouse Hes2 gene,a homologue of Drosophila hairy and Enhancer of split." Genomics. (in press.). (1998)
[文献書誌] Ohtsuka,T.,Asahi,M.,Matsuura,N.,Kikuchi,H.,Hojo,M.,Kageyama,R.,Ohkubo,H.,and Hoshimaru,M.: "Regulated expression of neurogenic basic helix-loop-helix transcription factors during differentiation of the immortalized neuronal progenitor cell line HC2S2 into neurons." Cell Tissue Res.(in press.). (1998)
[文献書誌] 松居靖久: "生殖細胞の増殖、生存の制御機構" Human Cell. 10. 63-68 (1997)
[文献書誌] 松居靖久: "生殖細胞の永遠性を支えるメカニズム" 細胞工学. 16. 1768-1774 (1997)
[文献書誌] 松居靖久: "哺乳類生殖細胞の発生機構" 蛋白質核酸酵素. (印刷中).
[文献書誌] Watanabe,T.et al.: "Assignment of the murine protein kinase gene DLK to Chromosome 15 in the vicinity of bt/Koa locus by genetic linkage analysis." Genomics. 40. 375-376 (1997)
[文献書誌] Hidai,C.et al.: "Cloning and characterizaton of developmental endothelial locus-1:An embryonic endothelial cell protein that binds the avb3 integrin receptor." Gene Dev.12. 21-33 (1998)
[文献書誌] Matsui,Y.: "Regulation of germ cell death in mammalian gonads." APMIS. (印刷中).
[文献書誌] Matsui,Y.: "Developmental fate of the mouse germ cells." Int.J.Dev.Biol.(印刷中).
[文献書誌] Yamamoto,M.et al.: "Coordinated expression of Hoxa-11 and Hoxa-13 during limb muscle patterning." Development. 125. 1325-1335 (1998)
[文献書誌] Tamura,K.et al.: "Retinoic acid changes the proximodistal developmental competence and affinity of distal cells in the developing chick limb bud" Dev Biol. 188. 224-234 (1997)
[文献書誌] T.Takeuchi.: "A gene trap approach to identify genes that control development." Dev.Growth.Differ.39. 127-134 (1997)
[文献書誌] J.Motoyama,K.Kitajima,M.Kojima,S.Kondo & T.Takeuchi.: "Organogenesis of the liver,thymus,and spleen is affected in jumonji mutant mice." Mech.Dev.66. 27-37 (1997)
[文献書誌] T.Takeuchi.: "Mapping to mouse chromosome 13 of the jumonji gene." Genomics. 45. 240-242 (1997)
[文献書誌] K.Kitajima,M.Kojima,K.Nakajima,S.Kondo & T.Takeuchi: "Definitive but not primitive hematopoiesis is impaired in jumonji mutant mice." (Submitted.).
[文献書誌] Araki,K.et al.: "Targeted integration of DNA using mutant lox sites in embryonic stem cells." Nucleic Acid Res.25. 868-872 (1997)
[文献書誌] Kimura,S.et al.: "2.1 kb 5'-flanking region of the brain type dystrophin gene directs the expression of lacZ in the cerebral cortex,but not in the hippocampus." J.Neurol.Sci.147. 13-20 (1997)
[文献書誌] Araki,K.et al.: "Efficinecy of recombination by Cre transient expression in embryonic stem cells:comparison of various promoters." J.Biochem.122. 977-982 (1997)
[文献書誌] Sekimoto,T.et al.: "Region specific expression of murine Hox genes implies the Hox code-mediated patterning of the digestive tract." Genes to Cells.(in press). (1997)
[文献書誌] Higashi,Y.et al.: "Impairment of Tcell development in δ EF1 mutant mice." Journal of Experimental Medicine. 185. 1467-1479 (1997)
[文献書誌] Wakamatsu,Y.et al.: "Regulation of the neural crest cell fate by N-myc." Development. 124. 1953-1962 (1997)
[文献書誌] Sekido,R.et al.: "Two mechanisms in the action of repressor δ EF1." Genes to Cells. 2(印刷中). (1997)
[文献書誌] Takagi,T.et al.: "δ EF1 is required for normal skeleton Patterning." Development. 125. 21-32 (1998)
[文献書誌] Kurihara,Y.,Nagata,T.,Imai,T.,Hiwatashi,A.,Horiuchi,M.,Sakakibara,S.,Katahira,M.,Okano,H.and Uesugi,S.: "Structural properties and RNA-binding activities of two RNA-Recognition Motifs of a mouse neural RNA binding protein,mouse-Musashi-1." Gene. 186. 21-27 (1997)
[文献書誌] Okabe,M.and Okano,H.: "Two-step induction of chordotonal organ precursors in Drosophila embryogenesis." Development. 124. 1045-1053 (1997)
[文献書誌] Kume,S.,Yamamoto,A.,Inoue,T.,Muto,A.,Okano,H.and Mikoshiba,K.: "Developmental expression of lnositol 1,4,5-trisphosphate receptor and structural changes in the endoplasmic reticulum during oogenesis and meiotic maturation of Xenopus laevis." Dev.Biol.182. 228-239 (1997)
[文献書誌] Okabe.M.,Sawamoto,K.,Imai,T.,Sakakibara.S.,Yoshikawa,S.and Okano,H.: "Instinsic and extrinsic determinants regulating cell fate decision in developing nervous system." Dev.Neurosci.19. 9-16 (1997)
[文献書誌] Hisahara,S.,Shoji,S.,Okano,H.and Miura,M.: "ICE/CED-3 family executes oligodendrocyte apoptosis by tumor necrosis factor." J.Neurochem.69. 10-20 (1997)
[文献書誌] Konishi,Y.,Kobayashi,Y.,Kishimoto,T.,Makino,Y.,Miyawaki,A.,Furuichi,T.,Okano,H.,Mikoshiba,K.,and Tamura,T.: "Demonstration of an E-box and its CNS-related binding factors for transcriptional regulation of the mouse type1 inositol 1,4,5-triphosphate receptor gene." J.Neurochem.69. 476-484 (1997)
[文献書誌] Kume,S.,Muto,A.,Okano,H.and Mikoshiba,K.: "Developmental expression of the inositol 1,4,5-trisphosphate receptor and a concentrated localization of inositol 1,4,5-trisphosphate during early embryogensis in Xenopus Laevis." Mech.Dev.66. 157-168 (1997)
[文献書誌] Sakakibara,S.and Okano,H.: "Expression of neural RNA-binding proteins in the post-natal CNS:implication of their roles in neural and glial cells development." J.Neurosci.17. 8300-8312 (1997)
[文献書誌] Kume,S.,Muto,A.,Inoue,T.,Suga,H.,Okano,H.and Mikoshiba,K.: "Role of inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos." Science. 278. 1940-1943 (1997)
[文献書誌] Shiraiwa,N.,Okano,H.and Miura,M.: "Bcl-2 prevents cell death downstream of CPP32 processing." Biomedical Journal. 18. 405-411 (1997)
[文献書誌] Sawamoto,K.,Taguchi,A.,Jin,M.,Yamada,C.and Okano,H.: "Argos induces programmed cell death in the developing Drosophila eye by inhibition of the Ras pathway." Cell Death and Differentiation in press. (印刷中). (1998)
[文献書誌] Hamada,S.,Senzaki,K.,Tabuchi,K.,Hamaguchi-Hamada,K.,Yamamoto,H.,Yamamoto,T.,Yoshikawa,S.,Okano,H.and Okado,N.: "Localization of 5-HT2A receptor in rat cerebral cortex and olfactory system revealed by immunohistochemistry using two antibodies raised in rabbit and chicken." Mol.Brain Res.54. 199-211 (1998)
[文献書誌] Pincus,D.W.,Keyoung,H.,Edgar,M.,Restelli,C.,Goodman,R.R.,Sakakibara,S.,Okano,H.,Fraser,R.A.R.Nedergaard,M.and Goldman,S.A.: "FGF2/BDNF-responsive neuronal progenitor cells in the adult human subependyma." Annals of Neurology in press. (印刷中). (1998)
[文献書誌] Hisahara,S.,Shoji,S.,Yoshikawa,S.,Okano,H.and Miura,M.: "Functional expression of bcl-2 family gene in Drosophila cells." J.Cell Sci.111. 667-673 (1998)
[文献書誌] Okano,H.,Okabe,M.,Taguchi,A.and Sawamoto,K.: "Evolutionarily conserved mechanisms of the regulation of neural development:lessons from the development of Drosophila peripheral nervous system." Human Cell. 10. 139-149 (1997)
[文献書誌] Miura,M.,Hisahara,S.,Araki,T.and Okano,H.: "Execution mechanisms of programmed cell death by caspase(ICE/CED-3)family proteases." Heart Vessels. 12. 66-70 (1997)
[文献書誌] Kikuchi,Y.,Segawa,H.,Tokumoto,M.,Tsubokawa,T.,Hotta,Y.,Uyemura,K.,and Okamoto,H.: "Functional analysis of Islet-3 LIM/homeodomain protein in zebrafish brain development.p73-87 in Molecular Basis of Axon Growth and Nerve Pattern Formation." Japan Scientific Societies Press, 280 (1997)
[文献書誌] Ikeya,M.,Lee,S.M.K.,McMahon,A.P.& Takada,S.: "in "Frontiers of Neural Development"" Springer-Verlag(in press),
[文献書誌] Okano,H.,Kaneko,Y.,Nakamura,Y.,Tabuchi,K.,Taguchi,A.,Okabe,M.,Akamatsu,W.,Yuasa,Y.,Imai,T.,Sakakibara,S.and Sawamoto,K.: "Frontiers of Neural Development" Springer-Verlag Tokyo,in press., (1998)
[文献書誌] Matsuno,K.,Ito,M.,Kishi,N.,Suzuki,S.,Artavanis-Tsakonas,S.and Okano,H.: "Frontiers of Neural Development" Springer-Verlag Tokyo,in press., (1998)
[文献書誌] Sawa,H.and Okano,H.: "Frontiers of Neural Development" Springer-Verlag Tokyo,in press., (1998)

1997 年度 実績報告書

発生・形態形成の個体レベルにおける分子生物学的研究

研究代表者

竹市 雅俊 京都大学, 大学院・理学研究科, 教授 (00025454)

研究成果

[文献書誌] Oda,H.,Uemura,T.,Takeichi,M.: "Phenotypic analysis of null mutants for DE-cadherin and Armadillo in Drosophila ovaries reveals distinct aspects of their functions in cell adhesion and cytoskeletal organization." Genes to Cells. 2. 29-40

[文献書誌] Inoue,T.,Chisaka,O.,Takeichi,M.et al.: "Cadherin-6 expression transiently delineates specific rhombomeres,other neural tube subdivisions and neural crest subpopulations in mouse embryos." Develop.Biol.183. 183-194

[文献書誌] Fushimi,D.,Arndt,K.,Takeichi,M.et al.: "Cloning and expression analysis of cadherin-10 in the CNS.of the chicken embryos." Develop.Dynamics. 209. 1-17

[文献書誌] Nose,A.,Umeda,T.,Takeichi,M.: "Neuromuscular target recognition by a homophilc inteaction of Connectin cell adhesion molecules in Drosophila." Development. 124. 1433-1441

[文献書誌] Radice,G.L.,Rayburn,H.,Takeichi,M.et al.: "Developmental defects in mouse embryos lacking N-cadherin." Develop.Biol.181. 64-78

[文献書誌] Umemiya,T.,Takeichi,M.,Nose,A.: "M-spondin,a novel ECM protein highly homologous to vertebrate F-spondin,is localized at the muscle attachment sites in the Drosophila embryos." Develop.Biol.186. 165-176

[文献書誌] Nakagawa,S.,Takeichi,M.: "N-cadherin is crucial for hear formation in the chick embryo." Develop.Growth Differ.39. 451-455

[文献書誌] Iwai,Y.,Usui,T.,Takeichi,M.et al.: "Axon patterning requires DN-cadherin,a novel neuronal adhesion receptor,in the Drosophila embryonic CNS." Neuron. 19. 77-89

[文献書誌] Isshiki,T.,Takeichi,M.,Nose,A.: "The role of the msh homeobox gene during neurogenesis:implication for the dorsoventral specification of the neuroectoderm." Development. 124. 3099-3109

[文献書誌] Suzuki,S.C.,Inoue,T.,Takeichi,M.et al.: "Neuronal circuits are subdivided by differential expression of type-II classic cadherins in postnatal mouse brains." Mol.Cell.Neuroscience. 9. 433-447

[文献書誌] Nose,A.,Isshiki,T.,Takeichi,M.: "Regional specification of muscle progenitors in Drosophila:the role of the msh homeobox gene." Development. 125. 215-223

[文献書誌] Inoue,T.,Tanaka,T.,Takeichi,M.et al.: "Cadherin-6 in the developing mouse brain:Expression along restricted connection systems and synaptic localization suggest a potential role in neuronal circuitry." Develop.Dynamics. (in press).

[文献書誌] Arndt,K.,Nakagawa,S.,Takeichi,M.et al.: "Cadherin-defined segment and parasagittal cell ribbons in the developing chicken cerebellum." Mol.Cell.Neuroscience. (in press).

[文献書誌] 能瀬聡直、梅田達夫、竹市雅俊: "Neuromuscular target recognition by a homophilic interaction of Connectin cell adhesion molecules in Drosophila." Development. 124. 1433-1441 (1997)

[文献書誌] 梅宮猛、竹市雅俊、能瀬聡直: "M-spondin,a novel ECM Protein highly homologous to vertebrate F-spondin is localized at the muscle attachment sites in the Drosophila embryo." Developmental Biology. 186. 165-175 (1997)

[文献書誌] 一色孝子、竹市雅俊、能瀬聡直: "The role of the msh homeobox gene during Drosophila neurogenesis;implication for the dorsoventral specification of the CNS." Development. 124. 3099-3109 (1997)

[文献書誌] 能瀬聡直、一色孝子、竹市雅俊: "Regional specification of muscle progenitors in Drosophila:the role of the msh homeobox gene." Development. 125. 215-223 (1998)

[文献書誌] 岡本 仁: "脳の複雑化と多様化" 現代化学. 11月号. 53-59 (1997)

[文献書誌] Kikuchi,Y.,Segawa,H.,Tokumoto,M.,Ttsubokawa,T.,Hotta,Y.,Uyemura,K.,and Okamoto,H.: "Ocular and cerebellar defects in zebrafish induced by overexpression of the LIM domain of the Islet-3 LIM/homeodomain protein." Neuron. 18. 369-382 (1997)

[文献書誌] Higashijima,S.,Nose,A.,Eguchi,G.,Hotta,Y.,and Okamoto,H.: "Mindin/F-spondin family:novel ECM proteins expressed in the zebrafish embryonic axis." Developmental Biology. 192. 211-277 (1997)

[文献書誌] Higashijima,S.,Okamoto,H.,Ueno,N.,Hotta,Y.,and Eguchi G.: "High-frequency generation of transgenic zebrafish which reliably express GFP in whole muscles or the whole body by using prooter of zebrafish origin." Developmental Biology. 192. 289-299 (1998)

[文献書誌] Hajime Ogino,Kunio Yasuda: "Induction of lens differntiation by activation of a bZIP transcription factor L-Maf" Science. (in press).

[文献書誌] Takuya Murata,Masahiro Nitta,Kunio Yasuda: "Involvement of the CP2 transcription factor family in lens-specific expression of the chicken A-crystallin gene." (submitted).

[文献書誌] Masahi Kitamura,Kumi Miyauchi,Kunio Yasuda: "Purification and immunological analyses of a nuclear factor,CEF1,that binds to a lens-specific enhnacer of the chicken A-crystallin" (submitted).

[文献書誌] Takebayashi,K.,Takahashi,S.,Yokota,C.,Tsuda,H.,Nakanishi,S.,Asashima,M.,and Kageyama,R.: "Conversion of ectoderm into a neural fate by ATH-3,a vertebrate basic helix-loop-helix gene homologous to Drosophila proneural gene atonal." EMBO J.16. 384-395 (1997)

[文献書誌] Matsue,M.,Kageyama,R.,Denhardt,D.T.,and Noda,M.: "Helix-loop-helix-type transcription factor(HES-1)is expressed in osteoblastic cells,suppressed by 1,25(OH)2 vitamin D3,and modulates 1,25(OH)2 vitamin D3 enhancement of osteopontin gene expression." Bone. 20. 329-334 (1997)

[文献書誌] Kageyama,R.and Nakanishi,S.: "Helix-loop-helix factors in growth and differentiation of the nervous system." Curr.Op.Genet.Dev.7. 659-665 (1997)

[文献書誌] Kageyama,R.,Ishibashi,M.,Takebayashi,K.,and Tomita,K.: "bHLH transcription factors and mammalian neuronal differentiation." Int.J.Biochem.Cell Biol.(in press). (1998)

[文献書誌] Ikeya,M.,Lee,S.M.K.,Johnson,J.E.,McMahon,A.P.& Takada,s.: "Wnt signalling required for expansion of neural crest and CNS progenitors." Nature. 389. 966-970 (1997)

[文献書誌] Yoshikawa,Y.,Fujimori,T.,McMahon,A.P.& Takada,S.: "Evidence that absence of Wnt-3a signaling promotes neuralization instead of paraxial mesoderm development in the mouse." Dev.Biol.183. 234-242 (1997)

[文献書誌] Nagai,T.,Aruga,J.,Takada,S.,Gunther,T.,Sporle,R.,Schughart,K.& Mikoshiba,K.: "The expression of the mouse Zic1,Zic2,and Zic3 gene suggests an essential role for Zic genes in body pattern formation." Dev.Biol.182. 299-313 (1997)

[文献書誌] Tsuda,H.,Takebayashi,K.,Nakanishi,S.,and Kageyama,R.: "Structure and promoter analysis of Math3 gene,a mouse homolog of Drosophila proneural gene atonal:neural-specific expression by dual promoter elements." J.Biol.Chem.(in press.). (1998)

[文献書誌] Nishimura,M.,Isaka,F.,Ishibashi,M.,Tomita,K.,Tsuda,H.,Nakanishi,S.,and Kageyama,R.: "Structure,chromosomal locus and promoter of mouse Hes2 gene,a homologue of Drosophila hairy and Enhancer of split." Genomics. (in press.). (1998)

[文献書誌] 松居靖久: "生殖細胞の増殖、生存の制御機構" Human Cell. 10. 63-68 (1997)

[文献書誌] 松居靖久: "生殖細胞の永遠性を支えるメカニズム" 細胞工学. 16. 1768-1774 (1997)

[文献書誌] 松居靖久: "哺乳類生殖細胞の発生機構" 蛋白質核酸酵素. (印刷中).

[文献書誌] Watanabe,T.et al.: "Assignment of the murine protein kinase gene DLK to Chromosome 15 in the vicinity of bt/Koa locus by genetic linkage analysis." Genomics. 40. 375-376 (1997)

[文献書誌] Hidai,C.et al.: "Cloning and characterizaton of developmental endothelial locus-1:An embryonic endothelial cell protein that binds the avb3 integrin receptor." Gene Dev.12. 21-33 (1998)

[文献書誌] Matsui,Y.: "Regulation of germ cell death in mammalian gonads." APMIS. (印刷中).

[文献書誌] Matsui,Y.: "Developmental fate of the mouse germ cells." Int.J.Dev.Biol.(印刷中).

[文献書誌] Yamamoto,M.et al.: "Coordinated expression of Hoxa-11 and Hoxa-13 during limb muscle patterning." Development. 125. 1325-1335 (1998)

[文献書誌] Tamura,K.et al.: "Retinoic acid changes the proximodistal developmental competence and affinity of distal cells in the developing chick limb bud" Dev Biol. 188. 224-234 (1997)

[文献書誌] T.Takeuchi.: "A gene trap approach to identify genes that control development." Dev.Growth.Differ.39. 127-134 (1997)

[文献書誌] J.Motoyama,K.Kitajima,M.Kojima,S.Kondo & T.Takeuchi.: "Organogenesis of the liver,thymus,and spleen is affected in jumonji mutant mice." Mech.Dev.66. 27-37 (1997)

[文献書誌] T.Takeuchi.: "Mapping to mouse chromosome 13 of the jumonji gene." Genomics. 45. 240-242 (1997)

[文献書誌] K.Kitajima,M.Kojima,K.Nakajima,S.Kondo & T.Takeuchi: "Definitive but not primitive hematopoiesis is impaired in jumonji mutant mice." (Submitted.).

[文献書誌] Araki,K.et al.: "Targeted integration of DNA using mutant lox sites in embryonic stem cells." Nucleic Acid Res.25. 868-872 (1997)

[文献書誌] Kimura,S.et al.: "2.1 kb 5'-flanking region of the brain type dystrophin gene directs the expression of lacZ in the cerebral cortex,but not in the hippocampus." J.Neurol.Sci.147. 13-20 (1997)

[文献書誌] Araki,K.et al.: "Efficinecy of recombination by Cre transient expression in embryonic stem cells:comparison of various promoters." J.Biochem.122. 977-982 (1997)

[文献書誌] Sekimoto,T.et al.: "Region specific expression of murine Hox genes implies the Hox code-mediated patterning of the digestive tract." Genes to Cells.(in press). (1997)

[文献書誌] Higashi,Y.et al.: "Impairment of Tcell development in δ EF1 mutant mice." Journal of Experimental Medicine. 185. 1467-1479 (1997)

[文献書誌] Wakamatsu,Y.et al.: "Regulation of the neural crest cell fate by N-myc." Development. 124. 1953-1962 (1997)

[文献書誌] Sekido,R.et al.: "Two mechanisms in the action of repressor δ EF1." Genes to Cells. 2(印刷中). (1997)

[文献書誌] Takagi,T.et al.: "δ EF1 is required for normal skeleton Patterning." Development. 125. 21-32 (1998)

[文献書誌] Kurihara,Y.,Nagata,T.,Imai,T.,Hiwatashi,A.,Horiuchi,M.,Sakakibara,S.,Katahira,M.,Okano,H.and Uesugi,S.: "Structural properties and RNA-binding activities of two RNA-Recognition Motifs of a mouse neural RNA binding protein,mouse-Musashi-1." Gene. 186. 21-27 (1997)

[文献書誌] Okabe,M.and Okano,H.: "Two-step induction of chordotonal organ precursors in Drosophila embryogenesis." Development. 124. 1045-1053 (1997)

[文献書誌] Kume,S.,Yamamoto,A.,Inoue,T.,Muto,A.,Okano,H.and Mikoshiba,K.: "Developmental expression of lnositol 1,4,5-trisphosphate receptor and structural changes in the endoplasmic reticulum during oogenesis and meiotic maturation of Xenopus laevis." Dev.Biol.182. 228-239 (1997)

[文献書誌] Okabe.M.,Sawamoto,K.,Imai,T.,Sakakibara.S.,Yoshikawa,S.and Okano,H.: "Instinsic and extrinsic determinants regulating cell fate decision in developing nervous system." Dev.Neurosci.19. 9-16 (1997)

[文献書誌] Hisahara,S.,Shoji,S.,Okano,H.and Miura,M.: "ICE/CED-3 family executes oligodendrocyte apoptosis by tumor necrosis factor." J.Neurochem.69. 10-20 (1997)

[文献書誌] Kume,S.,Muto,A.,Okano,H.and Mikoshiba,K.: "Developmental expression of the inositol 1,4,5-trisphosphate receptor and a concentrated localization of inositol 1,4,5-trisphosphate during early embryogensis in Xenopus Laevis." Mech.Dev.66. 157-168 (1997)

[文献書誌] Sakakibara,S.and Okano,H.: "Expression of neural RNA-binding proteins in the post-natal CNS:implication of their roles in neural and glial cells development." J.Neurosci.17. 8300-8312 (1997)

[文献書誌] Kume,S.,Muto,A.,Inoue,T.,Suga,H.,Okano,H.and Mikoshiba,K.: "Role of inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos." Science. 278. 1940-1943 (1997)

[文献書誌] Shiraiwa,N.,Okano,H.and Miura,M.: "Bcl-2 prevents cell death downstream of CPP32 processing." Biomedical Journal. 18. 405-411 (1997)

[文献書誌] Sawamoto,K.,Taguchi,A.,Jin,M.,Yamada,C.and Okano,H.: "Argos induces programmed cell death in the developing Drosophila eye by inhibition of the Ras pathway." Cell Death and Differentiation in press. (印刷中). (1998)

[文献書誌] Pincus,D.W.,Keyoung,H.,Edgar,M.,Restelli,C.,Goodman,R.R.,Sakakibara,S.,Okano,H.,Fraser,R.A.R.Nedergaard,M.and Goldman,S.A.: "FGF2/BDNF-responsive neuronal progenitor cells in the adult human subependyma." Annals of Neurology in press. (印刷中). (1998)

[文献書誌] Hisahara,S.,Shoji,S.,Yoshikawa,S.,Okano,H.and Miura,M.: "Functional expression of bcl-2 family gene in Drosophila cells." J.Cell Sci.111. 667-673 (1998)

[文献書誌] Okano,H.,Okabe,M.,Taguchi,A.and Sawamoto,K.: "Evolutionarily conserved mechanisms of the regulation of neural development:lessons from the development of Drosophila peripheral nervous system." Human Cell. 10. 139-149 (1997)

[文献書誌] Miura,M.,Hisahara,S.,Araki,T.and Okano,H.: "Execution mechanisms of programmed cell death by caspase(ICE/CED-3)family proteases." Heart Vessels. 12. 66-70 (1997)

[文献書誌] Kikuchi,Y.,Segawa,H.,Tokumoto,M.,Tsubokawa,T.,Hotta,Y.,Uyemura,K.,and Okamoto,H.: "Functional analysis of Islet-3 LIM/homeodomain protein in zebrafish brain development.p73-87 in Molecular Basis of Axon Growth and Nerve Pattern Formation." Japan Scientific Societies Press, 280 (1997)

[文献書誌] Ikeya,M.,Lee,S.M.K.,McMahon,A.P.& Takada,S.: "in "Frontiers of Neural Development"" Springer-Verlag(in press),

[文献書誌] Okano,H.,Kaneko,Y.,Nakamura,Y.,Tabuchi,K.,Taguchi,A.,Okabe,M.,Akamatsu,W.,Yuasa,Y.,Imai,T.,Sakakibara,S.and Sawamoto,K.: "Frontiers of Neural Development" Springer-Verlag Tokyo,in press., (1998)

[文献書誌] Matsuno,K.,Ito,M.,Kishi,N.,Suzuki,S.,Artavanis-Tsakonas,S.and Okano,H.: "Frontiers of Neural Development" Springer-Verlag Tokyo,in press., (1998)

[文献書誌] Sawa,H.and Okano,H.: "Frontiers of Neural Development" Springer-Verlag Tokyo,in press., (1998)

1997 年度実績報告書

竹市雅俊京都大学, 大学院・理学研究科, 教授 (00025454)

[文献書誌] 岡本仁: "脳の複雑化と多様化" 現代化学. 11月号. 53-59 (1997)