1998 年度実績報告書

外分泌における水電解質輸送と開口分泌の協調機構

研究課題

研究課題/領域番号	10044334
研究種目	国際学術研究
応募区分	共同研究
研究機関	岡崎国立共同研究機構
研究代表者	村上政隆岡崎国立共同研究機構, 生理学研究所, 助教授 (10104275)
研究分担者	TURNER R.Jam アメリカ国立衛生院(NIH), 主幹 DISSING Stee コペンハーゲン大学, 医学部, 助教授 YOUNG John A シドニー大学, 医学部, 助教授瀬川彰久北里大学, 医学部, 講師 (50154638) 杉谷博士日本大学, 松戸歯学部, 助教授 (20050114)
キーワード	水分泌 / 電解質輸送 / 蛋白分泌 / 開口放出 / 唾液腺 / 輸送体 / チャンネル / 細胞内情報伝達
研究概要	本研究は、大量の水分泌と開口分泌を観察できる唾液腺に対象を限定、小胞輸送により運ばれた分泌顆粒が細胞膜に融合し開口放出を起こすプロセスについて共同研究者の各実験サイトで分子生理的、形態学的に水電解質輸送と開口分泌を計測、水輸送・開口分泌両システム間の協調機構を検討することを目的とする。平成10年度、 A. 分泌経過チーム:酵素開口分泌(漿液分泌)のモデルとして、耳下腺の血管潅流・単離細胞標本を作製し、カルバコール(ムスカリニック刺激)イソプロテレノール(βアドレナジック刺激)について水電解質分泌・アミラーゼ分泌・酸素消費の時間経過を測定、水分泌と開口分泌が、前者は主として細胞内Ca信号によりコントロールされ、後者はCaとcyclic AMP両者の組み合わせで制御を受けることが明らかになった。 B. 形態チームは、レーザー・走査電子顕微鏡を用い、Aのプロトコルで、耳下腺の血管潅流・単離細胞標本の「分泌顆粒、小胞輸送、膜融合、開口放出、膜の回収」の時間経過に沿って水経路の形態の時間的変化とアミラーゼ開口分泌の微小形態の変化を観察した。その結果カルバコール刺激では大きな形態変化は無く、細胞間小管の拡大がみられるのみであった、一方開口分泌像はカルバコールとイドプロテレノール両者の刺激で多く観察され、貯蔵された分泌顆粒もその数を減少させた。 C. 開口放出関連蛋白チームは、耳下腺分泌顆粒膜の安定化を検討した。また、ターゲットの細胞膜における局在を、形態グループと協力して実施するために、新しいSEM法の開発に着手した。 D. 分泌物質の運動性グループは、本年は、水分泌経路のサイズの測定のため研究協力者ヒル博士(ケンブリッジ大と共同実験を行い標識デキストランの腺房上皮通過を測定した。本年度、アミラーゼ分泌増加と対応した酸素消費増加分を蛋白分泌に必要なエネルギー要求として測定できた。

研究成果
(42件)

すべてその他

すべて文献書誌 (42件)

[文献書誌] Yoshimura,K: "Cyclic AMP potentiates substance P-induced amylase secretion by augmenting the effect of calcium in the rat parotid acinar cells." Biochim Biophys Acta. 1402. 171-187 (1998)
[文献書誌] Murakami M: "Energy Consumption associated with fluid and electrolyte transport in the mandibular and parotid glands." Eur J Morphology. 36 Suppl. 121-131 (1998)
[文献書誌] Furuyama F: "Differences in Thermal salivation between the FOK rat (a model of genotypic heat adaptation) and three other rat strains." Physiology & Behavior. 63. 787-793 (1998)
[文献書誌] Fukuoka M: "Magnetic resonance imaging of experimental subacute spinal cord compression." SPINE. 23. 1540-1549 (1998)
[文献書誌] Seo Y: "NMR studies of salivary glands : from energy metabolism to morphology." Eur J Morphology. 36 Suppl. 98-102 (1998)
[文献書誌] Steward MC: "Cell volume regulation and diffusion of intracellular water in salivary acinar cells." Eur J Morphology. 36 Suppl. 103-106 (1998)
[文献書誌] A Riva: "Human salivary gland parenchymal cells seen by SEM from the cytoplasmic side using a new osmium maceration method." Eur J Morphology. 36 Suppl. 35-40 (1998)
[文献書誌] A Segawa: "Cytoskeletal regulation of human salivary secretion studied by high resolution electron microscopy and confocal laser microscopy." Eur J Morphology. 36 Suppl. 41-45 (1998)
[文献書誌] A Segawa: "Exocytosis in human salivary glands visualized by high-resolution scanning electron microscopy." Cell Tissue Res. 291. 325-336 (1998)
[文献書誌] A Segawa: "Exocytosis-removal cycle single secretory granules visualized by confocal laser microscopy." Bloimages. 5. 153-155 (1997)
[文献書誌] M Yamamoto-Hino: "Apical vesicles bearing inositol 1,4,5-triphosphate receptors in the Ca2+ initiation site of ductal epithlium of submandibular gland." J Cell Biol. 141. 135-142 (1998)
[文献書誌] Michikawa H: "cGMP production is coupled to Ca2+-dependent nitric oxide generation in rabbit parotid acinar cells." Cell Calcium. 23. 405-412 (1998)
[文献書誌] Dohke Y: "Translocation of Arf1 to the secretory granules in rat parotid acinar cells." Arch.Biochem.Biophys.357. 147-154 (1998)
[文献書誌] Fujita-Yoshigaki J: "SNARE proteins essential for cylic AMP-regulated exocytosis in salivary glands." Eur.J.Morphol.36suppl. 46-49 (1998)
[文献書誌] Dohke Y: "ADP-ribosylation factors in rat parotid acinar cells." Eur.J.Morphol.36suppl. 186-189 (1998)
[文献書誌] Sugiya H: "Ca2+-nitric oxide-cGMP signaling in rabbit parotid acinar cells." Eur.J.Morphol.36suppl. 194-197 (1998)
[文献書誌] Ogata Y: "Involvement of prostaglandins in histamine H1 receptor-operated Ca2+ entry in human gingival fibroblasts." Life Sci.64. 71-77 (1999)
[文献書誌] Y.Sharf: "Mapping strain on blood vessel walls using deuterium double-quantum-filtered MRI." Proc.Natl.Acad.Soc.USA. 95. 4108-4112 (1998)
[文献書誌] H.Yamada: "Selective detection of biexponential relaxation in isotropic solutions by mixed coherence filtering NMR." J.Magn.Reson.135. 99-103 (1998)
[文献書誌] L.Tsoref: "Proton double-quantum filtered MRI : A novel method for imaging ordered tissues." Magn.Reso.Med.40. 720-726 (1998)
[文献書誌] Y.Seo: "Anisotropic and restricted diffusion of water in sciatic nerve : A 2H double quantum filtered NMR study." Magn.Reso.Med.in press (1999)
[文献書誌] D.I.Cook: "Control of Na(+) transport in salivary duct epithelial cells by cytosolic Cl(-) and Na(+)" Eur.J.Morphol.36. 67-73 (1998)
[文献書誌] P.Komwatana: "Activators of epithelial Na(+) channels inhibit cytosolic feedback controls. Evidence for the existence of a G protein coupled receptor for cytosolic Na(+)" J.Membr.Biol.162. 225-232 (1998)
[文献書誌] A.Dinudom: "Nedd4 mediates control of an epithelial Na(+) channnel by cytosolic Na(+)" Proc.Natl.Acad.Sci.U.S.A.95. 7169-7173 (1998)
[文献書誌] D.W.Johnson: "Physiological changes in extracellular sodium directly control human proximal tubule growth and transport" Pfluegers Arch.435. 211-218 (1998)
[文献書誌] M.L.Day: "A cytoplasmic cell cycle controls the activity of a K(+) channel in preimplantation mouse embryos" EMBO J.17. 1952-1960 (1998)
[文献書誌] D.W.Johnson: "TGFbeta1 dissociates human proximal tubule cell growth and Na(+)-H(+) exchange activity" Kidney International. 53. 1601-1607 (1998)
[文献書誌] M.L.Day: "Cell cycle regulation of a T-type calcium current in early mouse embryos" Pfluegers Arch.436. 834-842 (1998)
[文献書誌] P.Poronnik: "Use of replication deficient adenoviruses to investigate the role of G proteins in Ca(2+) signalling in epithelial cells" Cell Calcium. 24. 97-103 (1998)
[文献書誌] Ishiguro H.: "Fluid secretion in interlobular ducts isolated from guinea-pig" J.Physiol.511. 407-422 (1998)
[文献書誌] Gresz V.: "Expression of aquaporin 1 (AQP1) water channels in human abial salivary glands." Arch.Oral Biol.in press. (1998)
[文献書誌] Seo J.T.: "Caffeine does not inhibit substance P-evoked intracellular Ca2+ mobilization in rat salivary acinar cells." Amer.J.Physiol.in press. (1998)
[文献書誌] Moore-Hoon,M.L.: "Molecular characterization of the cation-chloride cotransporter family." Eur.J.Morphology. 36Suppl. 137-141 (1998)
[文献書誌] Evans,R.L.: "New insights into upreguration and function of the salivary Na+-K+-2Cl contransporter." Eur.J.Morphology. 36Suppl. 142-146 (1998)
[文献書誌] Moore-Hoon,M.L.: "Increased expression of the secretory Na+-K+-2Cl- contransporter with differentiation of a human intestinal cell line." Biochem.Biophys.Res.Comm.244. 15-19 (1998)
[文献書誌] Evans,R.L.: "Evidence for a physiological role of NH4+ transport on the secretory Na+-K+-2Cl- cotranspoter." Biochem.Biophys.Res.Comm.245. 301-306 (1998)
[文献書誌] Seki Y: "Fast MR imaging of esophageal motility. in “Spatially Resolved Magnetic Resonance"" (eds.Bluemler P, Bluemich RB, Fukushima E), (395-401) (1998)
[文献書誌] Segawa A: "The dynamics of exocytosis of preformed secretory granules fro salivary glands. in “Glandular Mechanisms of Salivary Secretion"" (Garrett JR, Ekstrom J, Anderson LC eds) Frontiers of Oral Biology Vol 10, Karger (Basel), (89-100) (1998)
[文献書誌] A Segawa: "Exocytosis in living salivary glands. in “Microscopy, Image Analysis, and 3D Reconstruction CD-ROM"Vol1" (Pawley JW, Robinson Jp eds) Purdue University Cytometry Laboratories(USA), (1998)
[文献書誌] A Segawa: "Measurement of secretion. in Methods in Enzymology" Academic Press (in press), (1999)
[文献書誌] Y.Seo: "MMR imaging of rigid biological tissues. in : Spatially Resolved Magnetic Resonance" (edited by P.Bluemler, B.Bluemich, R.Botto, E.Fukushima) Weiley-VCH, Weinheim, (445-457) (1998)
[文献書誌] H.Shinar: "2H double quantum filtered NMR histology and diffusion measur isolated nerves and blood vessels.in : Spatially Resolved Magnetic Resonance" (edited by P.Bluemler, B.Bluemich, R.Botto, E.Fukushima) Weiley-VCH, Weinheim, (307-321) (1998)

1998 年度 実績報告書

外分泌における水電解質輸送と開口分泌の協調機構

研究代表者

村上 政隆 岡崎国立共同研究機構, 生理学研究所, 助教授 (10104275)

研究成果

[文献書誌] Yoshimura,K: "Cyclic AMP potentiates substance P-induced amylase secretion by augmenting the effect of calcium in the rat parotid acinar cells." Biochim Biophys Acta. 1402. 171-187 (1998)

[文献書誌] Murakami M: "Energy Consumption associated with fluid and electrolyte transport in the mandibular and parotid glands." Eur J Morphology. 36 Suppl. 121-131 (1998)

[文献書誌] Furuyama F: "Differences in Thermal salivation between the FOK rat (a model of genotypic heat adaptation) and three other rat strains." Physiology & Behavior. 63. 787-793 (1998)

[文献書誌] Fukuoka M: "Magnetic resonance imaging of experimental subacute spinal cord compression." SPINE. 23. 1540-1549 (1998)

[文献書誌] Seo Y: "NMR studies of salivary glands : from energy metabolism to morphology." Eur J Morphology. 36 Suppl. 98-102 (1998)

[文献書誌] Steward MC: "Cell volume regulation and diffusion of intracellular water in salivary acinar cells." Eur J Morphology. 36 Suppl. 103-106 (1998)

[文献書誌] A Riva: "Human salivary gland parenchymal cells seen by SEM from the cytoplasmic side using a new osmium maceration method." Eur J Morphology. 36 Suppl. 35-40 (1998)

[文献書誌] A Segawa: "Cytoskeletal regulation of human salivary secretion studied by high resolution electron microscopy and confocal laser microscopy." Eur J Morphology. 36 Suppl. 41-45 (1998)

[文献書誌] A Segawa: "Exocytosis in human salivary glands visualized by high-resolution scanning electron microscopy." Cell Tissue Res. 291. 325-336 (1998)

[文献書誌] A Segawa: "Exocytosis-removal cycle single secretory granules visualized by confocal laser microscopy." Bloimages. 5. 153-155 (1997)

[文献書誌] M Yamamoto-Hino: "Apical vesicles bearing inositol 1,4,5-triphosphate receptors in the Ca2+ initiation site of ductal epithlium of submandibular gland." J Cell Biol. 141. 135-142 (1998)

[文献書誌] Michikawa H: "cGMP production is coupled to Ca2+-dependent nitric oxide generation in rabbit parotid acinar cells." Cell Calcium. 23. 405-412 (1998)

[文献書誌] Dohke Y: "Translocation of Arf1 to the secretory granules in rat parotid acinar cells." Arch.Biochem.Biophys.357. 147-154 (1998)

[文献書誌] Fujita-Yoshigaki J: "SNARE proteins essential for cylic AMP-regulated exocytosis in salivary glands." Eur.J.Morphol.36suppl. 46-49 (1998)

[文献書誌] Dohke Y: "ADP-ribosylation factors in rat parotid acinar cells." Eur.J.Morphol.36suppl. 186-189 (1998)

[文献書誌] Sugiya H: "Ca2+-nitric oxide-cGMP signaling in rabbit parotid acinar cells." Eur.J.Morphol.36suppl. 194-197 (1998)

[文献書誌] Ogata Y: "Involvement of prostaglandins in histamine H1 receptor-operated Ca2+ entry in human gingival fibroblasts." Life Sci.64. 71-77 (1999)

[文献書誌] Y.Sharf: "Mapping strain on blood vessel walls using deuterium double-quantum-filtered MRI." Proc.Natl.Acad.Soc.USA. 95. 4108-4112 (1998)

[文献書誌] H.Yamada: "Selective detection of biexponential relaxation in isotropic solutions by mixed coherence filtering NMR." J.Magn.Reson.135. 99-103 (1998)

[文献書誌] L.Tsoref: "Proton double-quantum filtered MRI : A novel method for imaging ordered tissues." Magn.Reso.Med.40. 720-726 (1998)

[文献書誌] Y.Seo: "Anisotropic and restricted diffusion of water in sciatic nerve : A 2H double quantum filtered NMR study." Magn.Reso.Med.in press (1999)

[文献書誌] D.I.Cook: "Control of Na(+) transport in salivary duct epithelial cells by cytosolic Cl(-) and Na(+)" Eur.J.Morphol.36. 67-73 (1998)

[文献書誌] P.Komwatana: "Activators of epithelial Na(+) channels inhibit cytosolic feedback controls. Evidence for the existence of a G protein coupled receptor for cytosolic Na(+)" J.Membr.Biol.162. 225-232 (1998)

[文献書誌] A.Dinudom: "Nedd4 mediates control of an epithelial Na(+) channnel by cytosolic Na(+)" Proc.Natl.Acad.Sci.U.S.A.95. 7169-7173 (1998)

[文献書誌] D.W.Johnson: "Physiological changes in extracellular sodium directly control human proximal tubule growth and transport" Pfluegers Arch.435. 211-218 (1998)

[文献書誌] M.L.Day: "A cytoplasmic cell cycle controls the activity of a K(+) channel in preimplantation mouse embryos" EMBO J.17. 1952-1960 (1998)

[文献書誌] D.W.Johnson: "TGFbeta1 dissociates human proximal tubule cell growth and Na(+)-H(+) exchange activity" Kidney International. 53. 1601-1607 (1998)

[文献書誌] M.L.Day: "Cell cycle regulation of a T-type calcium current in early mouse embryos" Pfluegers Arch.436. 834-842 (1998)

[文献書誌] P.Poronnik: "Use of replication deficient adenoviruses to investigate the role of G proteins in Ca(2+) signalling in epithelial cells" Cell Calcium. 24. 97-103 (1998)

[文献書誌] Ishiguro H.: "Fluid secretion in interlobular ducts isolated from guinea-pig" J.Physiol.511. 407-422 (1998)

[文献書誌] Gresz V.: "Expression of aquaporin 1 (AQP1) water channels in human abial salivary glands." Arch.Oral Biol.in press. (1998)

[文献書誌] Seo J.T.: "Caffeine does not inhibit substance P-evoked intracellular Ca2+ mobilization in rat salivary acinar cells." Amer.J.Physiol.in press. (1998)

[文献書誌] Moore-Hoon,M.L.: "Molecular characterization of the cation-chloride cotransporter family." Eur.J.Morphology. 36Suppl. 137-141 (1998)

[文献書誌] Evans,R.L.: "New insights into upreguration and function of the salivary Na+-K+-2Cl contransporter." Eur.J.Morphology. 36Suppl. 142-146 (1998)

[文献書誌] Moore-Hoon,M.L.: "Increased expression of the secretory Na+-K+-2Cl- contransporter with differentiation of a human intestinal cell line." Biochem.Biophys.Res.Comm.244. 15-19 (1998)

[文献書誌] Evans,R.L.: "Evidence for a physiological role of NH4+ transport on the secretory Na+-K+-2Cl- cotranspoter." Biochem.Biophys.Res.Comm.245. 301-306 (1998)

[文献書誌] Seki Y: "Fast MR imaging of esophageal motility. in “Spatially Resolved Magnetic Resonance"" (eds.Bluemler P, Bluemich RB, Fukushima E), (395-401) (1998)

[文献書誌] Segawa A: "The dynamics of exocytosis of preformed secretory granules fro salivary glands. in “Glandular Mechanisms of Salivary Secretion"" (Garrett JR, Ekstrom J, Anderson LC eds) Frontiers of Oral Biology Vol 10, Karger (Basel), (89-100) (1998)

[文献書誌] A Segawa: "Exocytosis in living salivary glands. in “Microscopy, Image Analysis, and 3D Reconstruction CD-ROM"Vol1" (Pawley JW, Robinson Jp eds) Purdue University Cytometry Laboratories(USA), (1998)

[文献書誌] A Segawa: "Measurement of secretion. in Methods in Enzymology" Academic Press (in press), (1999)

[文献書誌] Y.Seo: "MMR imaging of rigid biological tissues. in : Spatially Resolved Magnetic Resonance" (edited by P.Bluemler, B.Bluemich, R.Botto, E.Fukushima) Weiley-VCH, Weinheim, (445-457) (1998)

[文献書誌] H.Shinar: "2H double quantum filtered NMR histology and diffusion measur isolated nerves and blood vessels.in : Spatially Resolved Magnetic Resonance" (edited by P.Bluemler, B.Bluemich, R.Botto, E.Fukushima) Weiley-VCH, Weinheim, (307-321) (1998)

1998 年度実績報告書

村上政隆岡崎国立共同研究機構, 生理学研究所, 助教授 (10104275)