2001 年度実績報告書

造血細胞の遺伝子操作

研究課題

研究課題/領域番号	10181105
研究機関	自治医科大学
研究代表者	小澤敬也自治医科大学, 医学部, 教授 (30137707)
研究分担者	吉田進昭東京大学, 医科学研究科, 教授 (10250341) 北村俊雄東京大学, 医科学研究科, 教授 (20282527) 谷憲三朗九州大学, 生体防御医学研究所, 教授 (00183864) 竹田潤二大阪大学, 医学部, 教授 (50163407) 生田宏一京都大学, 医学研究科, 助教授 (90193177)
キーワード	遺伝子治療 / 造血幹細胞 / 選択的増幅遺伝子 / コモンマーモセット / レトロウイルスベクター / 突然変異マウス / サイトカインレセプター / トランスポゾンシステム
研究概要	体細胞あるいは生殖系列細胞の遺伝子操作を軸とした造血システムの研究を行った。 [造血幹細胞の直接的遺伝子操作] 1. 遺伝子導入造血幹細胞の選択的増幅システムの開発(小澤):新しいタイプの選択的増幅遺伝子として、分子スイッチにエリスロポエチン受容体を用いるものを開発し、従来型より効率が良いことを確認した。 2. 小型霊長類造血幹細胞の特徴化と遺伝子導入(谷):抗コモンマーモセットCD34単クローン抗体をクローン化した。GFP発現HIV(VSV)シュードタイプレンチウィルスベクターを用いて、コモンマーモセットCD34陽性細胞に遺伝子導入し、NOD/SCIDマウスへの移植実験を行った。 3. 幹細胞への効率の良い遺伝子導入法の開発(北村):ネコ内因性レトロウイルスRD114のenvを利用した新しいパッケイジング細胞PLAT-Fを樹立した。この系で作製したウイルスベクターで臍帯血由来造血細胞に遺伝子導入し、NOD/SCIDマウスへの移植実験を行った。 [発生工学的アプローチによる造血システムの解析] 1. 発生工学的手法を用いた造血・血管系システムの解析(吉田):リンパ浮腫を呈する突然変異マウスにおける血管からリンパ管への血流の移行に関して、小腸粘膜絨毛の毛細血管から中心リンパ管への移行に端を発している可能性が高いことが判明した。 2. IL-7Rのリンパ球増殖・分化シグナル(生田):IL-7レセプターのシグナル伝達分子のSTAT5が5'Jg1プロモーター領域に転写共役因子をリクルートし、ヒストンのアセチル化を介してTCRγ遺伝子座のクロマチン構造変換を促し、転写とDNA組換えを誘導することを示した。 3. マウストランスポゾンシステムを利用した造血系新規遺伝子の探索(竹田):個体内で効率よく移動するマウストランスポゾンを利用して網羅的遺伝子機能解析システムの樹立を推進した。

研究成果

(65件)

すべてその他

すべて文献書誌 (65件)

[文献書誌] Kirito, K.: "Identification of the human erythropoietin receptor region required for Stat1 and Stat3 activation"Blood. (in press).
[文献書誌] Kirito, K.: "Constitutive activation of Stat1 and Stat3 in primary erythroleukemia cells"Int.J.Hematol.. (in press).
[文献書誌] Kirito, K.: "TPO regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol-3-kinase activation pathways"J.Biol.Chem.. (in press).
[文献書誌] Kishino, K.: "Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation"Leuk.Res.. 26. 13-17 (2002)
[文献書誌] Ohtsuki, T.: "Fanconi anemia protein, FANCA, associates with BRG1, a component of the human SWI/SNF complex"Hum.Mol.Genet.. 10. 2651-2660 (2001)
[文献書誌] Yamashita, Y.: "Sak serine/threonine kinase acts as an effector of Tec tyrosine kinase"J.Biol.Chem.. 276. 39012-39020 (2001)
[文献書誌] Muramatsu, M.: "Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells"Biochem.Biophys.Res.Commun.. 27. 891-896 (2001)
[文献書誌] Miyazato, A.: "Identification of myelodysplastic syndrome-specific genes by DNA microarray analysis with purified hematopoietic stem cell fraction"Blood. 98. 422-427 (2001)
[文献書誌] Kogure, K.: "Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components"Int.J.Hematol.. 73. 469-475 (2001)
[文献書誌] Mori, M.: "Antitumor effect of beta2-microglobulin in leukemic cell-bearing mice via apoptosis-inducing activity : activation of caspase-3 and nuclear factor-kappaB"Cancer Res.. 61. 4414-4417 (2001)
[文献書誌] Tanaka, M.: "Forkhead family transcription factor FKHRL1 is expressed in human megakaryocytes and regulates cell cycling as a downstream molecule of TPO signaling"J.Biol.Chem.. 276. 15082-15089 (2001)
[文献書誌] Hanazono, Y.: "Gene transfer into nonhuman primate hematopoietic stem cells : implications for gene therapy"Stem Cells. 19. 12-23 (2001)
[文献書誌] Uchida, M.: "A functional role of mitogen-activated protein kinases, Erk1 and Erk2, in the differentiation of a human leukemia cell line, UT-7/GM : A possible key factor for cell fate determination toward erythroid and megakaryocytic lineages"Int.J.Hematol.. 73. 78-83 (2001)
[文献書誌] Shinjyo, T.: "Downregulation of Bim, a proapoptotic relative of Bcl-2, is a pivotal step in cytokine-initiated survival signaling in murine hematopoietic progenitors"Mol.Cell.Biol.. 21. 854-864 (2001)
[文献書誌] Tabata, M.: "Stimulation of GATA-2 as a mechanism of hydrogen peroxide suppression in hypoxia-induced erythropoietin gene expression"J.Cell.Physiol.. 186. 260-267 (2001)
[文献書誌] Ooi, J.: "Unrelated cord blood translplantation for adult patients with MDS-related secondary acute myeloid leukaemia"Br.J.Haematol.. (in press).
[文献書誌] Ooi, J.: "Successful unrelated cord blood transplantation for relapse after autologous transplantation in non-Hodgkin's lymphoma"Leuk.Lymphoma. (in press).
[文献書誌] Tomonari, A.: "Severe autoimmune thrombocytopenia after allogeneic bone marrow transplantation for aplastic anemia"Int.J.Hematol.. (in press).
[文献書誌] Duda, D.G.: "Overexpression of the p53-induced brain angiogenesis inhibitor 1 suppresses efficiently tumor angiogenesis"Br.J.Cancer. (in press).
[文献書誌] Nagayama, H.: "Transient hematopoietic stem cell rescue using umbilical cord blood for a lethally irradiated nuclear accident victim"Bone Marrow Transplant.. (in press).
[文献書誌] Kawai, K.: "Clinical course of an advanced renal cell carcinoma patient treated with GM-CSF gene therapy(GVAX) : The first experience of cancer gene therapy in Japan"Int.J.Urol.. (in press).
[文献書誌] Ohata, J.: "Differential graft-versus-leukemia effect by CD28 and CD40 costimulatory blockade after graft versus-host disease prophylaxis"Clin.Exp.Immunol.. (in press).
[文献書誌] Hibino, H.: "Haematopoietic progenitor cells from common marmoset as targets of gene transduction by retroviral and adenoviral vectors"Eur.J.Haematol.. 66. 272-280 (2001)
[文献書誌] Inazawa, T.: "Glucorticoid-regulated expression of exogenous human growth hormone gene in rats"Mol.Ther.. 4. 267-272 (2001)
[文献書誌] Kuwabara, T.: "Allosterically controllable maxizyme-mediated suppression of progression of leukemia in mice"Biomacromolecules. 2. 1220-1228 (2001)
[文献書誌] Ooi, J.: "Successful allogeneic bone marrow transplantation for hepatosplenic γδ T cell lymphoma"Hematologica. 86. E25 (2001)
[文献書誌] Tulin, E.E.: "Inhibition of human endothelial cell proliferation by ShIF, a vacuolar H+-ATPAse-like protein"Oncogene. (in press).
[文献書誌] Nosaka, T.: "Pim-1 expression is sufficient to induce cytokine independence in murin hematopoietic cells, but is dispensable for BCR-ABL-mediated transformation"Exp.Hematol.. (in press).
[文献書誌] Nakamura, T.: "Cytokine receptor common b subunit-mediated STAT5 activation confers NFkB activation through IkB-independent mechanism in a murine pro B cell line Ba/F3"J.Biol.Chem.. (in press).
[文献書誌] Kitamura, T.: "Role of MgcRacGAP/CYK4 as a regulator of the small GTPase Rho family in cytokinesis and cell differentiation"Cell Structure and Function. (in press).
[文献書誌] Kagami, S.: "Role of stat5a in T helper cell differentiation"Blood. 97. 2358-2365 (2001)
[文献書誌] Hirose, K.: "MgcRacGAP is involved in cytokinesis through associating with mitotic spindle and midbody"J.Biol.Chem.. 276. 5821-5828 (2001)
[文献書誌] Kamizono, S.: "The SOCS box of SOCS-1 accelerates ubiquitin-dependent proteolysis of TEL-JAK2"J.Biol.Chem.. 276. 12530-12538 (2001)
[文献書誌] Kawashima, T.: "STAT5 induces macrophage differentiation of M1 leukemia cells through activation of IL-6 production mediated by NF-kB p65"J.Immunol.. 167. 3652-3660 (2001)
[文献書誌] Tonozuka, Y.: "Molecular cloning of a human novel type I cytokine receptor related to Delta1"Cytogenet.Cell Genet.. 93. 23-25 (2001)
[文献書誌] Ishido, M.: "Cholesterol requirement for receptor transport from multivesicular late endosomes to the Golgi"J.Cell Science. 114. 1965-1976 (2001)
[文献書誌] Tulin, E.E.: "A novel secreted form of immune suppressor factor with high homology to vacuolar ATPase identified by forward genetic approach of functional screening based on cell proliferation"J.Biol.Chem.. 276. 27519-27526 (2001)
[文献書誌] Kimura, M.: "Regulation of Th2 cell differentiation by mel-18, a mammalian polycomb group gene"Immunity. 15. 275-287 (2001)
[文献書誌] Senga, T.: "JAK/Stat3 dependent activation of RalGDS/Ral pathway in M1 mouse myeloid leukemic cells"J.Biol.Chem.. 276. 32678-32681 (2001)
[文献書誌] Frasor, J.: "Differential roles for Stat5a and Stat5b in prolactin stimulation of estrogen receptor a and b transcription"Mol.Endcrinol.. 15. 2172-2181 (2001)
[文献書誌] Yamamoto, S.: "Proleferation of parenchymal neural progenitors in response to injury in the adult rat spinal cord"Exp.Neurol.. 172. 115-127 (2001)
[文献書誌] Tokoro, Y.: "Molecular cloning and characterization of mouse tspan-3, a novel member of the tetraspanin superfamily, expressed on resting dendritic cells"Biochem.Biophys.Res.Commun.. 288. 178-183 (2001)
[文献書誌] Ueno, M.: "Identification of an embryonic dorsal aorta specific gene, endomucin, and its inhibitory activity on cell adhesion"Biochem.Biophys.Res.Commun.. 287. 501-506 (2001)
[文献書誌] Yamamoto, S.: "Transcription factor expression and Notch-dependent regulation of neural progenitors in the adult rat spinal cord"J.Neurosci.. 21. 9814-9823 (2001)
[文献書誌] Tulin, E.E.: "SF20, a novel bone marrow stroma-derived factor that binds to mouse TSA-1 and supports lymphoid cell proliferation"J.Immunol.. 167. 6338-6347 (2001)
[文献書誌] Arakawa, M.: "Negamycin can restore dystrophin in mdx sskeletal muscle"Acta Myologica. 20. 154-158 (2001)
[文献書誌] Tulin, E.E.: "Genetic approach and phenotype-based complementation screening for identification of stromal cell-derived proteins involved in cell proliferation"Exp.Cell Res.. 272. 23-31 (2002)
[文献書誌] Perez, O.D.: "Acitivation of the PKB/AKT pathway by ICAM-2"Immunity. 16. 51-65 (2002)
[文献書誌] Honke, K.: "Paranodal junction formation and spermatogenesis require sulfoglycolipids"Proc.Natl.Acad.Sci.USA. (in press).
[文献書誌] Ohtsuka, S.: "SH2-B is Required for both Male and Female Reproduction"Mol.Cell.Biol.. (in press).
[文献書誌] Fukuyama, H.: "Requirement of Fas expression in B cells for tolerance induction"Eur.J.Immunol.. 32. 223-230 (2002)
[文献書誌] Fukami, K.: "Requirement of phosholipase Cd4 for the first step of fertilization"Science. 292. 920-923 (2001)
[文献書誌] Hirata, A.: "Abnormalities of Synapses and Neurons in the Hippocampus of Neuropsin-Deficient Mice"Mol.Cell.Neurosci.. 17. 600-610 (2001)
[文献書誌] Lee, H.-C.: "Seeding of dendritic epidermal T cells in the neonatal skin is reduced in 129 strain of mice"Immunol.Lett.. (in press).
[文献書誌] Honda, K.: "Molecular basis for hematopoietic/mesenchymal interaction during initiation of Peyer's patch organogenesis"J.Exp.Med.. 193. 621-630 (2001)
[文献書誌] Agata, Y.: "Histone acetylation determines the developmentally regulated accessibility for T cell receptor-γ gene recombination"J.Exp.Med.. 193. 873-879 (2001)
[文献書誌] Lee, H.-C.: "Induction of germline transcription in the human T cell receptor γ locus by STAT5"J.Immunol.. 167. 320-326 (2001)
[文献書誌] Ohba, Y.: "Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis"EMBO J.. 20. 3333-3341 (2001)
[文献書誌] Ye, S.-K.: "Differential roles of cytokine receptors in the development of epidermal γ δ T cells"J.Immunol.. 167. 1929-1934 (2001)
[文献書誌] Fagarasan, S.: "In situ class switching and differentiation to IgA-producing cells in the gut lamina propria"Nature. 413. 639-643 (2001)
[文献書誌] Ye, S.-K.: "The IL-7 receptor controls the accessibility of the TCRγ locus by Stat5 and histone acetylation"Immunity. 15. 813-823 (2001)
[文献書誌] Nishimura, J.: "Efficient retrovirus-mediated PIG-A gene transfer and stable restoration of GPI-anchored protein expression in cells with the PNH phenotype"Blood. 97. 3004-3010 (2001)
[文献書誌] Kawane, K.: "Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver"Science. 292. 1546-1549 (2001)
[文献書誌] Horie, K.: "Efficient chromosomal transposition of a Tc1/mariner-like transposon Sleeping Beauty in mice"Proc.Natl.Acad.Sci.USA. 98. 9191-9196 (2001)
[文献書誌] Sano, S.: "Stat3 in thymic epithelial cells is essential for postnatal maintainance of thymic architecture and thymocyte survival"Immunity. 15. 261-273 (2001)

2001 年度 実績報告書

造血細胞の遺伝子操作

研究代表者

小澤 敬也 自治医科大学, 医学部, 教授 (30137707)

研究成果

[文献書誌] Kirito, K.: "Identification of the human erythropoietin receptor region required for Stat1 and Stat3 activation"Blood. (in press).

[文献書誌] Kirito, K.: "Constitutive activation of Stat1 and Stat3 in primary erythroleukemia cells"Int.J.Hematol.. (in press).

[文献書誌] Kirito, K.: "TPO regulates Bcl-xL gene expression through Stat5 and phosphatidylinositol-3-kinase activation pathways"J.Biol.Chem.. (in press).

[文献書誌] Kishino, K.: "Evaluation of engraftment by ABO genotypic analysis of erythroid burst-forming units after bone marrow transplantation"Leuk.Res.. 26. 13-17 (2002)

[文献書誌] Ohtsuki, T.: "Fanconi anemia protein, FANCA, associates with BRG1, a component of the human SWI/SNF complex"Hum.Mol.Genet.. 10. 2651-2660 (2001)

[文献書誌] Yamashita, Y.: "Sak serine/threonine kinase acts as an effector of Tec tyrosine kinase"J.Biol.Chem.. 276. 39012-39020 (2001)

[文献書誌] Muramatsu, M.: "Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells"Biochem.Biophys.Res.Commun.. 27. 891-896 (2001)

[文献書誌] Miyazato, A.: "Identification of myelodysplastic syndrome-specific genes by DNA microarray analysis with purified hematopoietic stem cell fraction"Blood. 98. 422-427 (2001)

[文献書誌] Kogure, K.: "Targeted integration of foreign DNA into a defined locus on chromosome 19 in K562 cells using AAV-derived components"Int.J.Hematol.. 73. 469-475 (2001)

[文献書誌] Mori, M.: "Antitumor effect of beta2-microglobulin in leukemic cell-bearing mice via apoptosis-inducing activity : activation of caspase-3 and nuclear factor-kappaB"Cancer Res.. 61. 4414-4417 (2001)

[文献書誌] Tanaka, M.: "Forkhead family transcription factor FKHRL1 is expressed in human megakaryocytes and regulates cell cycling as a downstream molecule of TPO signaling"J.Biol.Chem.. 276. 15082-15089 (2001)

[文献書誌] Hanazono, Y.: "Gene transfer into nonhuman primate hematopoietic stem cells : implications for gene therapy"Stem Cells. 19. 12-23 (2001)

[文献書誌] Uchida, M.: "A functional role of mitogen-activated protein kinases, Erk1 and Erk2, in the differentiation of a human leukemia cell line, UT-7/GM : A possible key factor for cell fate determination toward erythroid and megakaryocytic lineages"Int.J.Hematol.. 73. 78-83 (2001)

[文献書誌] Shinjyo, T.: "Downregulation of Bim, a proapoptotic relative of Bcl-2, is a pivotal step in cytokine-initiated survival signaling in murine hematopoietic progenitors"Mol.Cell.Biol.. 21. 854-864 (2001)

[文献書誌] Tabata, M.: "Stimulation of GATA-2 as a mechanism of hydrogen peroxide suppression in hypoxia-induced erythropoietin gene expression"J.Cell.Physiol.. 186. 260-267 (2001)

[文献書誌] Ooi, J.: "Unrelated cord blood translplantation for adult patients with MDS-related secondary acute myeloid leukaemia"Br.J.Haematol.. (in press).

[文献書誌] Ooi, J.: "Successful unrelated cord blood transplantation for relapse after autologous transplantation in non-Hodgkin's lymphoma"Leuk.Lymphoma. (in press).

[文献書誌] Tomonari, A.: "Severe autoimmune thrombocytopenia after allogeneic bone marrow transplantation for aplastic anemia"Int.J.Hematol.. (in press).

[文献書誌] Duda, D.G.: "Overexpression of the p53-induced brain angiogenesis inhibitor 1 suppresses efficiently tumor angiogenesis"Br.J.Cancer. (in press).

[文献書誌] Nagayama, H.: "Transient hematopoietic stem cell rescue using umbilical cord blood for a lethally irradiated nuclear accident victim"Bone Marrow Transplant.. (in press).

[文献書誌] Kawai, K.: "Clinical course of an advanced renal cell carcinoma patient treated with GM-CSF gene therapy(GVAX) : The first experience of cancer gene therapy in Japan"Int.J.Urol.. (in press).

[文献書誌] Ohata, J.: "Differential graft-versus-leukemia effect by CD28 and CD40 costimulatory blockade after graft versus-host disease prophylaxis"Clin.Exp.Immunol.. (in press).

[文献書誌] Hibino, H.: "Haematopoietic progenitor cells from common marmoset as targets of gene transduction by retroviral and adenoviral vectors"Eur.J.Haematol.. 66. 272-280 (2001)

[文献書誌] Inazawa, T.: "Glucorticoid-regulated expression of exogenous human growth hormone gene in rats"Mol.Ther.. 4. 267-272 (2001)

[文献書誌] Kuwabara, T.: "Allosterically controllable maxizyme-mediated suppression of progression of leukemia in mice"Biomacromolecules. 2. 1220-1228 (2001)

[文献書誌] Ooi, J.: "Successful allogeneic bone marrow transplantation for hepatosplenic γδ T cell lymphoma"Hematologica. 86. E25 (2001)

[文献書誌] Tulin, E.E.: "Inhibition of human endothelial cell proliferation by ShIF, a vacuolar H+-ATPAse-like protein"Oncogene. (in press).

[文献書誌] Nosaka, T.: "Pim-1 expression is sufficient to induce cytokine independence in murin hematopoietic cells, but is dispensable for BCR-ABL-mediated transformation"Exp.Hematol.. (in press).

[文献書誌] Nakamura, T.: "Cytokine receptor common b subunit-mediated STAT5 activation confers NFkB activation through IkB-independent mechanism in a murine pro B cell line Ba/F3"J.Biol.Chem.. (in press).

[文献書誌] Kitamura, T.: "Role of MgcRacGAP/CYK4 as a regulator of the small GTPase Rho family in cytokinesis and cell differentiation"Cell Structure and Function. (in press).

[文献書誌] Kagami, S.: "Role of stat5a in T helper cell differentiation"Blood. 97. 2358-2365 (2001)

[文献書誌] Hirose, K.: "MgcRacGAP is involved in cytokinesis through associating with mitotic spindle and midbody"J.Biol.Chem.. 276. 5821-5828 (2001)

[文献書誌] Kamizono, S.: "The SOCS box of SOCS-1 accelerates ubiquitin-dependent proteolysis of TEL-JAK2"J.Biol.Chem.. 276. 12530-12538 (2001)

[文献書誌] Kawashima, T.: "STAT5 induces macrophage differentiation of M1 leukemia cells through activation of IL-6 production mediated by NF-kB p65"J.Immunol.. 167. 3652-3660 (2001)

[文献書誌] Tonozuka, Y.: "Molecular cloning of a human novel type I cytokine receptor related to Delta1"Cytogenet.Cell Genet.. 93. 23-25 (2001)

[文献書誌] Ishido, M.: "Cholesterol requirement for receptor transport from multivesicular late endosomes to the Golgi"J.Cell Science. 114. 1965-1976 (2001)

[文献書誌] Tulin, E.E.: "A novel secreted form of immune suppressor factor with high homology to vacuolar ATPase identified by forward genetic approach of functional screening based on cell proliferation"J.Biol.Chem.. 276. 27519-27526 (2001)

[文献書誌] Kimura, M.: "Regulation of Th2 cell differentiation by mel-18, a mammalian polycomb group gene"Immunity. 15. 275-287 (2001)

[文献書誌] Senga, T.: "JAK/Stat3 dependent activation of RalGDS/Ral pathway in M1 mouse myeloid leukemic cells"J.Biol.Chem.. 276. 32678-32681 (2001)

[文献書誌] Frasor, J.: "Differential roles for Stat5a and Stat5b in prolactin stimulation of estrogen receptor a and b transcription"Mol.Endcrinol.. 15. 2172-2181 (2001)

[文献書誌] Yamamoto, S.: "Proleferation of parenchymal neural progenitors in response to injury in the adult rat spinal cord"Exp.Neurol.. 172. 115-127 (2001)

[文献書誌] Tokoro, Y.: "Molecular cloning and characterization of mouse tspan-3, a novel member of the tetraspanin superfamily, expressed on resting dendritic cells"Biochem.Biophys.Res.Commun.. 288. 178-183 (2001)

[文献書誌] Ueno, M.: "Identification of an embryonic dorsal aorta specific gene, endomucin, and its inhibitory activity on cell adhesion"Biochem.Biophys.Res.Commun.. 287. 501-506 (2001)

[文献書誌] Yamamoto, S.: "Transcription factor expression and Notch-dependent regulation of neural progenitors in the adult rat spinal cord"J.Neurosci.. 21. 9814-9823 (2001)

[文献書誌] Tulin, E.E.: "SF20, a novel bone marrow stroma-derived factor that binds to mouse TSA-1 and supports lymphoid cell proliferation"J.Immunol.. 167. 6338-6347 (2001)

[文献書誌] Arakawa, M.: "Negamycin can restore dystrophin in mdx sskeletal muscle"Acta Myologica. 20. 154-158 (2001)

[文献書誌] Tulin, E.E.: "Genetic approach and phenotype-based complementation screening for identification of stromal cell-derived proteins involved in cell proliferation"Exp.Cell Res.. 272. 23-31 (2002)

[文献書誌] Perez, O.D.: "Acitivation of the PKB/AKT pathway by ICAM-2"Immunity. 16. 51-65 (2002)

[文献書誌] Honke, K.: "Paranodal junction formation and spermatogenesis require sulfoglycolipids"Proc.Natl.Acad.Sci.USA. (in press).

[文献書誌] Ohtsuka, S.: "SH2-B is Required for both Male and Female Reproduction"Mol.Cell.Biol.. (in press).

[文献書誌] Fukuyama, H.: "Requirement of Fas expression in B cells for tolerance induction"Eur.J.Immunol.. 32. 223-230 (2002)

[文献書誌] Fukami, K.: "Requirement of phosholipase Cd4 for the first step of fertilization"Science. 292. 920-923 (2001)

[文献書誌] Hirata, A.: "Abnormalities of Synapses and Neurons in the Hippocampus of Neuropsin-Deficient Mice"Mol.Cell.Neurosci.. 17. 600-610 (2001)

[文献書誌] Lee, H.-C.: "Seeding of dendritic epidermal T cells in the neonatal skin is reduced in 129 strain of mice"Immunol.Lett.. (in press).

[文献書誌] Honda, K.: "Molecular basis for hematopoietic/mesenchymal interaction during initiation of Peyer's patch organogenesis"J.Exp.Med.. 193. 621-630 (2001)

[文献書誌] Agata, Y.: "Histone acetylation determines the developmentally regulated accessibility for T cell receptor-γ gene recombination"J.Exp.Med.. 193. 873-879 (2001)

[文献書誌] Lee, H.-C.: "Induction of germline transcription in the human T cell receptor γ locus by STAT5"J.Immunol.. 167. 320-326 (2001)

[文献書誌] Ohba, Y.: "Requirement for C3G-dependent Rap1 activation for cell adhesion and embryogenesis"EMBO J.. 20. 3333-3341 (2001)

[文献書誌] Ye, S.-K.: "Differential roles of cytokine receptors in the development of epidermal γ δ T cells"J.Immunol.. 167. 1929-1934 (2001)

[文献書誌] Fagarasan, S.: "In situ class switching and differentiation to IgA-producing cells in the gut lamina propria"Nature. 413. 639-643 (2001)

[文献書誌] Ye, S.-K.: "The IL-7 receptor controls the accessibility of the TCRγ locus by Stat5 and histone acetylation"Immunity. 15. 813-823 (2001)

[文献書誌] Nishimura, J.: "Efficient retrovirus-mediated PIG-A gene transfer and stable restoration of GPI-anchored protein expression in cells with the PNH phenotype"Blood. 97. 3004-3010 (2001)

[文献書誌] Kawane, K.: "Requirement of DNase II for definitive erythropoiesis in the mouse fetal liver"Science. 292. 1546-1549 (2001)

[文献書誌] Horie, K.: "Efficient chromosomal transposition of a Tc1/mariner-like transposon Sleeping Beauty in mice"Proc.Natl.Acad.Sci.USA. 98. 9191-9196 (2001)

[文献書誌] Sano, S.: "Stat3 in thymic epithelial cells is essential for postnatal maintainance of thymic architecture and thymocyte survival"Immunity. 15. 261-273 (2001)

2001 年度実績報告書

小澤敬也自治医科大学, 医学部, 教授 (30137707)