1998 年度実績報告書

組織・器官分化の分子機構

研究課題

研究課題/領域番号	10182102
研究機関	京都大学
研究代表者	町田泰則京都大学, 大学院・理学研究科, 教授 (80175596)
研究分担者	西村いくこ岡崎国立共同研究機構, 基礎生物学研究所, 助教授 (00241232) 西谷和彦東北大学, 大学院・理学研究科, 教授 (60164555) 中村研三名古屋大学, 大学院・生命農学研究科, 教授 (80164292) 塚谷裕一東京大学, 分子細胞生物学研究所, 助手 (90260512) 島本功奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10263427)
キーワード	NPK1 / MAPKKK / キネシン様タンパク質 / MAPKK / MAPキナーゼカスケード / M期 / 細胞周期 / 植物細胞
研究概要	本計画研究班の目的は、メリステムからの植物器官・組織の発生分化の分子的背景を様々な角度から研究することである。町田は、葉の扁平性と左右対称性について、シロイヌナズナの変異体であるale1とas2を用いて研究した。その結果、前者では表皮細胞の分化が、後者では維管束の分化が異常になっていることがわかった。ale1については遺伝子クローニングを行い、その塩基配列からサチライシンファミリー,に属するセリンプロテアーゼをコードしている可能性が示唆された。AS2遺伝子については、独自の方法により染色体歩行を行い、約25kbの中の6つのORFにまで絞り込んだ。河内は、栄養成長から生殖成長への転換の分子機構を知るために、低発現遺伝子のライブラリーから花序分裂組織特異的遺伝子のcDNAを選別した。それらのうちZinc-fingerタンパク質遺伝子のT-DNAによる破壊株などを単離・解析した結果、花の異常を見いだした。島本は、短日植物であるイネの光周性を研究し、光周性を完全になくした変異体se-5の原因遺伝子がHY1である可能性を示した。塚谷は、葉の長軸方向と横軸方向の成長の制御機構を研究してきた。クローニングしたROT遺伝子の発現系を用いて、これが葉の長軸方向への伸長に関わっていることを示した。中村は、しょ糖に対する植物の応答と器官分化の関連を研究するために、7500系統のアクテイベイションタギングライブラリーを作製し、その中に、糖に対する応答が異常になった変異体を見い出した。また、しよ糖誘導性遺伝子の最小プロモーターに存在する配列を調べ、糖に応答する結合タンパク質と複数のシスエレメント(10bp,SP8a,SP8b)を検出した。西谷は、タバコ培養細胞BY-2にEXGT cDNAアンティセンスを導入し、増殖速度の遅延と細胞形態の異常を見つけた。西村は、液胞へのタンパク質輸送系を研究して、これに関与している小胞(PAC)に存在するPV100がプロセシングされ複数の機能分子になることを示した。また、栄養器官型VPEが活性を持った分子として液胞で機能していることがわかった。

研究成果
(40件)

すべてその他

すべて文献書誌 (40件)

[文献書誌] Nakashima,M.: "The expression pattern of the gene for NPK1 protein kinase related to mitogen-activated protein kinase kinase kinase (MAPKKK) in a tobacco plant: Correlation with cell proliferation." Plant and Cell Physiology. 39. 690-700 (1998)
[文献書誌] Ito,M.: "M-phase specific transcription of plant B-type cycline genes is regulated by a cell-cycle dependent cis-acting activator element similar to mammalian Myb binding sites." The Plant Cell. 10. 331-341 (1998)
[文献書誌] Machida,Y.: "MAPKKK-related protein kinase NPK1: Regulation of the M phase of plant cell cycle." ournal of Plant Research. 111. 243-246 (1998)
[文献書誌] Bhalerao,R.P.: "Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases." Proc.Natl.Acad.Scie.USA. (受理). (1999)
[文献書誌] Muramoto,T.: "The Arabidopsis photomorphogenic mutant hy1 is deficient in phytochrome chromophore biosynthesis as a result of a mutation in a plastid heme oxygenase." Plant Cell. (印刷中). (1999)
[文献書誌] Kyozuka,J.: "Downregulation of RLF, the FLO/LFY homolog of rice, accompanied with panicle branching initiation." Proc.Natl.Acad.Sci.USA. 95. 1979-1982 (1998)
[文献書誌] Isshiki,M.: "A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron." Plant J.15. 133-138 (1998)
[文献書誌] Kyozuka,J.: "Role of RFL, the FLO/LFY homolog of rice (Oryza sativa L.) in panicle development." Flower.Newslett.26. 11-18 (1998)
[文献書誌] Morino,K.: "Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene." Plant J.17. 275-285 (1999)
[文献書誌] Takahashi,A.: "Lesion-mimic mutants of rice with alterations in early signaling events of defense." Plant J.(印刷中). (1999)
[文献書誌] Shimamoto,K.: "Programmed cell death in plants." Plant Biotech.(印刷中). (1999)
[文献書誌] Kim,G.-T.: "The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P450 family that is required for the regulated polar elongation of leaf cells." Genes & Dev.12. 2381-2391 (1998)
[文献書誌] Kim,G.-T.: "The CURLY LEAF gene controls both division and elongation of cells during the expansion of the leaf blade in Arabidopsis thaliana." Planta.206. 175-183 (1998)
[文献書誌] Okada,H.: "A new species of Schismatoglottis (SCHISMATOGLOTTIDINAE,ARACEAE) from West Kalimantan and observations of its peculiar bulbil development." Syst.Bot.(印刷中). (1999)
[文献書誌] Tsukaya,H.: "Genetic evidence for polarities that regulate leaf morphogenesis." J.Plant Res.111. 113-119 (1998)
[文献書誌] Tsukaya,H.: "Molecular mechanisms related to the evolution of leaf shape." Proceedings of IIAS International Symposium on “Biodiversity". (印刷中). (1999)
[文献書誌] 塚谷裕一: "葉の形作りのメカニズム" Academia. 175. 54-58 (1998)
[文献書誌] Imanishi,S.: "An mRNA of tobacco cell, which is rapidly inducible by methyl jasmonate in the presence of cycloheximide, codes for a putative glycosyltransferase." Plant Cell Physiol.39. 202-211 (1998)
[文献書誌] Iwata,Y.: "Characterization of a calcium-dependent protein kinase of tobacco leaves that is associated with the plasma membrane and is inducible bv sucrose." Plant Cell Physiol.39. 1176-1183 (1998)
[文献書誌] Mita,S.: "Differential expression of genes involved in the biosynthesis and perception of ethylene during ripening of passion fruit (Passiflora edulis Sims)." Plant Cell Physiol.39. 1209-1217 (1998)
[文献書誌] Imanishi,S.: "Jasmonate-induced accumulation of nicotine in tobacco cells occurs by non-coordinated induction of expression of genes for ornithine decarboxylase and other enzymes." Plant Mol.Biol.38. 1101-1111 (1998)
[文献書誌] Nishitani,K.: "Construction and restructuring of the cellulose-xyloglucan framework in the apoplast as mediated by the xyloglucan-related protein family- A hypothetical scheme" J.Plant Res.111. 159-166 (1998)
[文献書誌] Takano,M.: "Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots" Plant Cell Physiol.40. 135-142 (1999)
[文献書誌] Hara-Nishimura,I.: "Vacuolar processing enzyme in protein storage vacuoles and lytic vacuoles." J.Plant Physiol.152. 668-674 (1998)
[文献書誌] Hara-Nishimura,I.: "Transport of storage proteins to protein storage vacuoles is mediated by large precursor-accumulating vesicles." Plant Cell. 10. 825-836 (1998)
[文献書誌] Yamada,K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor by vacuolar processing enzyme." J.Biol.Chem.274. 2563-2570 (1998)
[文献書誌] Kato,A.: "Glyoxysomal malate dehydrogenase in pumpkin: cloning of a cDNA and functional analysis of its prosequence." Plant Cell Physiol.39. 186-195 (1998)
[文献書誌] Hiraiwa,N.: "Vacuolar processing enzyme is self-catalytically activated by sequential removal of the C-terminal and N-terminal propeptides." FEBS Let.(印刷中). (1999)
[文献書誌] Kinoshita,T.: "Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions." Plant J.(印刷中). (1999)
[文献書誌] Hayashi,M.: "Accumulation of a fusion protein containing 2S albumin induces novel vesicles in vegetative cells of Arabidopsis." Plant Cell Physiol.(印刷中). (1999)
[文献書誌] Koumoto,Y.: "Chloroplast Cpn20 forms a tetrameric structure in Arabidopsis thaliana." Plant J.(印刷中). (1999)
[文献書誌] Hara-Nishimura,I.: "Asparaginyl endopeptidase. In Handbook of Proteolytic Enzymes, edited by A.J.Barrett, N.D.Rawlings and J.F.Woessner" Academic Press, London, UK. 746-749 (1998)
[文献書誌] Machida,C.: "Use of the R-RS site-specific recombination system in plants.(印刷中)" Plant Molecular Biology Manual (ed.by Gelvin, S.B.) published by Kluwer Academic Publishers (Netherland), (1999)
[文献書誌] Nishihama,R.: "The MAP kinase cascade that includes MAPKKK-related protein kinase NPK1 controls a mitotic process in plant cells.(印刷中)" Plant MAP kinase cascades (ed.by Heri Hirt) published by Springer-Verlag Heidelberg (Germany), (1999)
[文献書誌] 町田泰則: "葉の形づくりにかかわる遺伝子、「植物の生長:遺伝子から何が見えるか」" 第12回「大学と科学」公開シンポジウム組織委員会編(クバプロ発行), 8 (1998)
[文献書誌] Shimamoto,K.: "Molecular Biology of Rice" Springer Verlag,(Berlin), (1999)
[文献書誌] Izawa,T.: "Ac/Ds as tools for rice molecular biology." Springer Verlag,(Berlin), 18 (1999)
[文献書誌] 塚谷裕一: "葉から花へ---花の進化と形態形成の遺伝子群" 大原雅編著『花の自然誌』/北大図書刊行会、第10章, 15 (1999)
[文献書誌] 西村いくこ: "種子ができるとき-変身する液胞,「植物の生長遺伝子から何が見えるか」" 第12回「大学と科学」公開シンポジウム組織委員会編(クバプロ発行), 9 (1998)
[文献書誌] 西村いくこ: "貯蔵たん白質の細胞内輸送と成熟化の機構の解析、大豆たん白質研究" 財団法人大豆たん白質研究振興財団(京都), 6 (1998)

1998 年度 実績報告書

組織・器官分化の分子機構

研究代表者

町田 泰則 京都大学, 大学院・理学研究科, 教授 (80175596)

研究成果

[文献書誌] Nakashima,M.: "The expression pattern of the gene for NPK1 protein kinase related to mitogen-activated protein kinase kinase kinase (MAPKKK) in a tobacco plant: Correlation with cell proliferation." Plant and Cell Physiology. 39. 690-700 (1998)

[文献書誌] Ito,M.: "M-phase specific transcription of plant B-type cycline genes is regulated by a cell-cycle dependent cis-acting activator element similar to mammalian Myb binding sites." The Plant Cell. 10. 331-341 (1998)

[文献書誌] Machida,Y.: "MAPKKK-related protein kinase NPK1: Regulation of the M phase of plant cell cycle." ournal of Plant Research. 111. 243-246 (1998)

[文献書誌] Bhalerao,R.P.: "Regulatory interaction of PRL1 WD protein with Arabidopsis SNF1-like protein kinases." Proc.Natl.Acad.Scie.USA. (受理). (1999)

[文献書誌] Muramoto,T.: "The Arabidopsis photomorphogenic mutant hy1 is deficient in phytochrome chromophore biosynthesis as a result of a mutation in a plastid heme oxygenase." Plant Cell. (印刷中). (1999)

[文献書誌] Kyozuka,J.: "Downregulation of RLF, the FLO/LFY homolog of rice, accompanied with panicle branching initiation." Proc.Natl.Acad.Sci.USA. 95. 1979-1982 (1998)

[文献書誌] Isshiki,M.: "A naturally occurring functional allele of the rice waxy locus has a GT to TT mutation at the 5' splice site of the first intron." Plant J.15. 133-138 (1998)

[文献書誌] Kyozuka,J.: "Role of RFL, the FLO/LFY homolog of rice (Oryza sativa L.) in panicle development." Flower.Newslett.26. 11-18 (1998)

[文献書誌] Morino,K.: "Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene." Plant J.17. 275-285 (1999)

[文献書誌] Takahashi,A.: "Lesion-mimic mutants of rice with alterations in early signaling events of defense." Plant J.(印刷中). (1999)

[文献書誌] Shimamoto,K.: "Programmed cell death in plants." Plant Biotech.(印刷中). (1999)

[文献書誌] Kim,G.-T.: "The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P450 family that is required for the regulated polar elongation of leaf cells." Genes & Dev.12. 2381-2391 (1998)

[文献書誌] Kim,G.-T.: "The CURLY LEAF gene controls both division and elongation of cells during the expansion of the leaf blade in Arabidopsis thaliana." Planta.206. 175-183 (1998)

[文献書誌] Okada,H.: "A new species of Schismatoglottis (SCHISMATOGLOTTIDINAE,ARACEAE) from West Kalimantan and observations of its peculiar bulbil development." Syst.Bot.(印刷中). (1999)

[文献書誌] Tsukaya,H.: "Genetic evidence for polarities that regulate leaf morphogenesis." J.Plant Res.111. 113-119 (1998)

[文献書誌] Tsukaya,H.: "Molecular mechanisms related to the evolution of leaf shape." Proceedings of IIAS International Symposium on “Biodiversity". (印刷中). (1999)

[文献書誌] 塚谷 裕一: "葉の形作りのメカニズム" Academia. 175. 54-58 (1998)

[文献書誌] Imanishi,S.: "An mRNA of tobacco cell, which is rapidly inducible by methyl jasmonate in the presence of cycloheximide, codes for a putative glycosyltransferase." Plant Cell Physiol.39. 202-211 (1998)

[文献書誌] Iwata,Y.: "Characterization of a calcium-dependent protein kinase of tobacco leaves that is associated with the plasma membrane and is inducible bv sucrose." Plant Cell Physiol.39. 1176-1183 (1998)

[文献書誌] Mita,S.: "Differential expression of genes involved in the biosynthesis and perception of ethylene during ripening of passion fruit (Passiflora edulis Sims)." Plant Cell Physiol.39. 1209-1217 (1998)

[文献書誌] Imanishi,S.: "Jasmonate-induced accumulation of nicotine in tobacco cells occurs by non-coordinated induction of expression of genes for ornithine decarboxylase and other enzymes." Plant Mol.Biol.38. 1101-1111 (1998)

[文献書誌] Nishitani,K.: "Construction and restructuring of the cellulose-xyloglucan framework in the apoplast as mediated by the xyloglucan-related protein family- A hypothetical scheme" J.Plant Res.111. 159-166 (1998)

[文献書誌] Takano,M.: "Endoxyloglucan transferase cDNA isolated from pea roots and its fluctuating expression in hydrotropically responding roots" Plant Cell Physiol.40. 135-142 (1999)

[文献書誌] Hara-Nishimura,I.: "Vacuolar processing enzyme in protein storage vacuoles and lytic vacuoles." J.Plant Physiol.152. 668-674 (1998)

[文献書誌] Hara-Nishimura,I.: "Transport of storage proteins to protein storage vacuoles is mediated by large precursor-accumulating vesicles." Plant Cell. 10. 825-836 (1998)

[文献書誌] Yamada,K.: "Multiple functional proteins are produced by cleaving Asn-Gln bonds of a single precursor by vacuolar processing enzyme." J.Biol.Chem.274. 2563-2570 (1998)

[文献書誌] Kato,A.: "Glyoxysomal malate dehydrogenase in pumpkin: cloning of a cDNA and functional analysis of its prosequence." Plant Cell Physiol.39. 186-195 (1998)

[文献書誌] Hiraiwa,N.: "Vacuolar processing enzyme is self-catalytically activated by sequential removal of the C-terminal and N-terminal propeptides." FEBS Let.(印刷中). (1999)

[文献書誌] Kinoshita,T.: "Vacuolar processing enzyme is up-regulated in the lytic vacuoles of vegetative tissues during senescence and under various stressed conditions." Plant J.(印刷中). (1999)

[文献書誌] Hayashi,M.: "Accumulation of a fusion protein containing 2S albumin induces novel vesicles in vegetative cells of Arabidopsis." Plant Cell Physiol.(印刷中). (1999)

[文献書誌] Koumoto,Y.: "Chloroplast Cpn20 forms a tetrameric structure in Arabidopsis thaliana." Plant J.(印刷中). (1999)

[文献書誌] Hara-Nishimura,I.: "Asparaginyl endopeptidase. In Handbook of Proteolytic Enzymes, edited by A.J.Barrett, N.D.Rawlings and J.F.Woessner" Academic Press, London, UK. 746-749 (1998)

[文献書誌] Machida,C.: "Use of the R-RS site-specific recombination system in plants.(印刷中)" Plant Molecular Biology Manual (ed.by Gelvin, S.B.) published by Kluwer Academic Publishers (Netherland), (1999)

[文献書誌] Nishihama,R.: "The MAP kinase cascade that includes MAPKKK-related protein kinase NPK1 controls a mitotic process in plant cells.(印刷中)" Plant MAP kinase cascades (ed.by Heri Hirt) published by Springer-Verlag Heidelberg (Germany), (1999)

[文献書誌] 町田 泰則: "葉の形づくりにかかわる遺伝子、「植物の生長:遺伝子から何が見えるか」" 第12回「大学と科学」公開シンポジウム組織委員会編(クバプロ発行), 8 (1998)

[文献書誌] Shimamoto,K.: "Molecular Biology of Rice" Springer Verlag,(Berlin), (1999)

[文献書誌] Izawa,T.: "Ac/Ds as tools for rice molecular biology." Springer Verlag,(Berlin), 18 (1999)

[文献書誌] 塚谷 裕一: "葉から花へ---花の進化と形態形成の遺伝子群" 大原雅 編著『花の自然誌』/北大図書刊行会、第10章, 15 (1999)

[文献書誌] 西村 いくこ: "種子ができるとき-変身する液胞,「植物の生長 遺伝子から何が見えるか」" 第12回「大学と科学」公開シンポジウム組織委員会編(クバプロ発行), 9 (1998)

[文献書誌] 西村 いくこ: "貯蔵たん白質の細胞内輸送と成熟化の機構の解析、大豆たん白質研究" 財団法人大豆たん白質研究振興財団(京都), 6 (1998)

1998 年度実績報告書

町田泰則京都大学, 大学院・理学研究科, 教授 (80175596)

[文献書誌] 塚谷裕一: "葉の形作りのメカニズム" Academia. 175. 54-58 (1998)

[文献書誌] 町田泰則: "葉の形づくりにかかわる遺伝子、「植物の生長:遺伝子から何が見えるか」" 第12回「大学と科学」公開シンポジウム組織委員会編(クバプロ発行), 8 (1998)

[文献書誌] 塚谷裕一: "葉から花へ---花の進化と形態形成の遺伝子群" 大原雅編著『花の自然誌』/北大図書刊行会、第10章, 15 (1999)

[文献書誌] 西村いくこ: "種子ができるとき-変身する液胞,「植物の生長遺伝子から何が見えるか」" 第12回「大学と科学」公開シンポジウム組織委員会編(クバプロ発行), 9 (1998)

[文献書誌] 西村いくこ: "貯蔵たん白質の細胞内輸送と成熟化の機構の解析、大豆たん白質研究" 財団法人大豆たん白質研究振興財団(京都), 6 (1998)