1999 年度実績報告書

遺伝的組換えの新展開:DNAの切断と再結合

研究課題

研究課題/領域番号	10216101
研究機関	(社)北里研究所
研究代表者	池田日出男 (社)北里研究所, 基礎研究所, 部長 (10012775)
研究分担者	小川英行岩手女子看護短期大学, 教授 (70028207) 関口睦夫福岡歯科大学, 歯学部, 教授 (00037342) 志村令郎北里研究所, 生物分子光学研究所, 所長 (60025426) 飯田滋北里研究所, 基礎生物学研究所, 教授 (30012777) 大坪栄一東京大学, 分子細胞生物学研究所, 教授 (10158800)
キーワード	DNAエンドジョイニング / 遺伝的組換え / DNA切断再結合 / DNAヘリカーゼ / シャフロン / エンドヌクレアーゼ / トランスポゾン / 遺伝子ホーミング
研究概要	本特定領域研究は、非相同的組換えに焦点を絞り、染色体のエンドジョイニングやトランスポゾンを含むいわゆる動く遺伝子の転移機構を「DNAの切断と再結合」という観点から総合的に理解しようとするものである。本年度は、11月に公開国際シンポジウム、2月に班会議を開催し、各計画研究間の連携を諮った。公開国際シンポジウムは、「非相同組換えの多様性:生物学から医科学へ」というタイトルのもとに国際的に活躍している3名の外国人研究者と3名の日本人研究者を迎えて、話題提供と活発な討論が行われた。参加者が100名を越える盛況となり、意義のある国際集会となった。また、班会議では、計画班員に加えて班友の小松賢志、能美健彦、総括班の関口睦夫によって最近の業績の紹介が行われた。昨年に比べて各班員の研究は著しく進展しており、この特定領域研究がこの領域の研究の発展に寄与しつつあることが明らかになった。この班会議と前後して、班員、或いは、班友の間でいくつかの共同研究も計画され、その一部も班会議において紹介された。班員の研究推進のために大型機器が購入され、今年度は北里研究所に設置された。今年度も、班員間の連絡のためにサーキュラーを発行し、また、研究業績の公開のためにホームページを更新し、公開している。今後も、班会議、メールによる連絡の強化、ホームページによる公開に加え、機械があればまた新しいテーマで公開シンポジウムを企画し、研究業績の公開を行いたいと考えている。

研究成果

(27件)

すべてその他

すべて文献書誌 (27件)

[文献書誌] Onda M., Hanada, K., Kawachi, H. and Ikeda, H.: "Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress"Genetics. 151. 439-446 (1999)
[文献書誌] Ashizawa, Y., Yokochi, T., Ogata, Y., Shobuike, Y., Kato, J., and Ikada, H.: "Mechanism of DNA gyrase-mediated illegitimate recombination : Characterization of Escherichia coli gyrA mutations that confer hyperrecombination phenotype"J. Mol. Biol.. 289. 447-458 (1999)
[文献書誌] Heo, S.-J., Tatebayashi, K. and Ikeda, H.: "The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC"Curr. Genet.. 36. 329-338 (1999)
[文献書誌] Yamashita, T., Hanada, K., Iwasaki, M., Yamaguchi, H. and Ikeda, H.: "Illegitimate recombination induced by overproduction of DnaB helicase in Eschierichia coli"J. Bacteriol.. 181. 4549-4553 (1999)
[文献書誌] Nohmi, T., Suzuki, M., Masumura, K., Yamada, M., Matsui, K., Ueda, O., Suzuki, H., Katoh, M., Ikeda, H., Sofuni, T.: "Spi(-) selection : An efficient method to detect gamma-ray-induced deletions in transgenic mice"Environ. Mol. Mutagen. 34. 9-15 (1999)
[文献書誌] Heo, S.-J., Tatebayashi, K., Ohsugi, I., Shimamoto, A., Furuichi, Y. and Ikeda, H.: "Bloom's syndrome gene suppresses premature aging caused by Sgs1 deficiency in yeast"Genes Cells. 4. 619-625 (1999)
[文献書誌] Yamaguchi, H., Haneda, K., Asami,Y., Kato, J. and Ikeda, H.: "Control of genetic stability in Escherichia coli : the SbcB 3'-5' exonuclease suppresses illegitimate recombination promoted by the RecE 5'-3' exonuclease"Genes Cells. 5. 101-109 (2000)
[文献書誌] Kang, S.-W. and Ikeda, H.: "Werner syndrome : Implications for aging and cancer. RN homologues in non-human systems"GANN MONOGRAPH ON CANCER RESEARCH. (印刷中).
[文献書誌] Kanaya, E., Nakajima, N., Morikawa, K., Okada, K., and Shimura, Y.: "Characterization of the transcription activator CBF1 from Arabidopsis thaliana"J. Biol. Chem.. 274. 16068-16076 (1999)
[文献書誌] Hanada, N., Nureki, O., Kurimoto, K., Kim, I., Sakamoto, H., Shimura, Y., Muto, Y., and Yokoyama, S.: "Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein"Nature. 398. 576-585 (1999)
[文献書誌] Sawa, S., Ito, T., Shimuka, Y., and Okada, K.: "FIL AMENTOUS FLOWER controls the formation and development of Arabidopsis inflorescences and floral meristems"Plant Cell. 11. 69-86 (1999)
[文献書誌] Kawate, H., Itoh, R., Sakumi, K., Nakabeppu, Y., Tsuzuki, T., Ide, F., Ishikawa., T., Noda, T., Nawata, H., and Sekiguchi, M.: "A defect in a single allele of the Mlh1 gene causes dissociation of the killing and tumorigenic actions of an alkylating carcinogen in methyltransferase-deficient mice"Carcinogenesis. 21. 301-305 (2000)
[文献書誌] Fujii, Y., Shimokawa, H., Sekiguchi, M., and Nakabeppu, Y.: "Functional significance of the conserved residues for the 23-residue module among MTH1 and MutT family proteins"J. Biol. Chem.. 274. 38251-38259 (1999)
[文献書誌] Oda, H., Taketomi, A., Maruyama, R., Itoh, R., Nishioka, K., Yakushiji, H., Suzuki, T., Sekiguchi, M., and Nakabeppu, Y.: "Multiforms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism"Nucleic Acids Res.. 27. 4335-4343 (1999)
[文献書誌] Sekiguchi, M., and Sanada, M.: "Alkylation carcinogenesis in mice with altered levels of DNA repair methyltransferase"Prog. Exp. Tumor Res.. 35. 25-36 (1999)
[文献書誌] Hayakawa, H., Hofer, A., Thelander, L., Kitajima, S., Cai, Y., Oshiro, S., Yakushiji, H., Nakebeppu, Y., Kuwano, M., and Sekiguchi, M.: "Metabolic fate of exidized guanine ribonucleotides in mammalian cells"Biochemistry. 38. 3610-3614 (1999)
[文献書誌] Nakagawa, T. and Ogawa, H.: "The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis"EMBO J.. 18. 5714-5723 (1999)
[文献書誌] Shiga, Y., Sekine, Y., and Ohtsubo, E.: "Transposition of IS1 circles"Genes Cells. 4. 551-561 (1999)
[文献書誌] Sekine, Y., Aihara, K., and Ohtsubo, E.: "Linearization and transposistion of circular molecules of insertion sequence IS3"J. Mol. Biol.. 294. 21-34 (1999)
[文献書誌] Noma, K., Ohtsubo, E., and Ohtsubo, H.: "Non-LTR retrotransposons (LINEs) as ubiquitous components of plant genomes"Mol. Gen. Genet.. 261. 71-79 (1999)
[文献書誌] Kumekawa, N., Ohtsubo, H., Horiuchi., T., and Ohtsubo, E.: "Identification and characterization of novel retrotransposons of the gypsy type in rice"Mol. Gen. Genet.. 260. 593-602 (1999)
[文献書誌] Hasebe, A. and Iida, S.: "The novel insertion sequences IS1417, IS1418 and IS1419 from Burkholderia glumae and their strain distribution"Plasmid. (印刷中).
[文献書誌] Iida, S., Hoshino, A., Johzuka-Hisatomi, Y., Habu Y. and Inagaki, Y.: "Floricultural traits and transposable elements in the Japanese and common morning glories"Annal. New York Acad. Sci.. 870. 265-274 (1999)
[文献書誌] Iida S. and Hoshino, A.: "Spontaneous mutagenesis and transposable elements in the Japanese morning glory"Gamma Field Symposia. (印刷中).
[文献書誌] Inagaki, Y., Johzuka-Hisatomi, Y., Mori, T., Takahashi, S., Hayakawa, Y., Peyachoknagul, S., Ozeki Y. and IIda, S.: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories"Gene. 226. 181-188 (1999)
[文献書誌] Johzuka-Hisatomi, Y., Hoshino, A., Mori, T., Habu Y. and IIda, S.: "Characterization of the chalcone synthase genes expressed in flowers of the common and Japanese morning glories"Genes Genet. Syst.. 74. 141-147 (1999)
[文献書誌] Shiokawa, K., Inagaki, Y., Morita, H., Hsu, T.-J., Iida S. and Noguchi,H.: "The functional expression of the CHS-D and CHS-E genes of the common morning glory (Ipomoea purpurea) in Escherichia coli and characterization of their gene products"Plant Biotechnology. (印刷中).

1999 年度 実績報告書

遺伝的組換えの新展開:DNAの切断と再結合

研究代表者

池田 日出男 (社)北里研究所, 基礎研究所, 部長 (10012775)

研究成果

[文献書誌] Onda M., Hanada, K., Kawachi, H. and Ikeda, H.: "Escherichia coli MutM suppresses illegitimate recombination induced by oxidative stress"Genetics. 151. 439-446 (1999)

[文献書誌] Ashizawa, Y., Yokochi, T., Ogata, Y., Shobuike, Y., Kato, J., and Ikada, H.: "Mechanism of DNA gyrase-mediated illegitimate recombination : Characterization of Escherichia coli gyrA mutations that confer hyperrecombination phenotype"J. Mol. Biol.. 289. 447-458 (1999)

[文献書誌] Heo, S.-J., Tatebayashi, K. and Ikeda, H.: "The budding yeast cohesin gene SCC1/MCD1/RHC21 genetically interacts with PKA, CDK and APC"Curr. Genet.. 36. 329-338 (1999)

[文献書誌] Yamashita, T., Hanada, K., Iwasaki, M., Yamaguchi, H. and Ikeda, H.: "Illegitimate recombination induced by overproduction of DnaB helicase in Eschierichia coli"J. Bacteriol.. 181. 4549-4553 (1999)

[文献書誌] Nohmi, T., Suzuki, M., Masumura, K., Yamada, M., Matsui, K., Ueda, O., Suzuki, H., Katoh, M., Ikeda, H., Sofuni, T.: "Spi(-) selection : An efficient method to detect gamma-ray-induced deletions in transgenic mice"Environ. Mol. Mutagen. 34. 9-15 (1999)

[文献書誌] Heo, S.-J., Tatebayashi, K., Ohsugi, I., Shimamoto, A., Furuichi, Y. and Ikeda, H.: "Bloom's syndrome gene suppresses premature aging caused by Sgs1 deficiency in yeast"Genes Cells. 4. 619-625 (1999)

[文献書誌] Yamaguchi, H., Haneda, K., Asami,Y., Kato, J. and Ikeda, H.: "Control of genetic stability in Escherichia coli : the SbcB 3'-5' exonuclease suppresses illegitimate recombination promoted by the RecE 5'-3' exonuclease"Genes Cells. 5. 101-109 (2000)

[文献書誌] Kang, S.-W. and Ikeda, H.: "Werner syndrome : Implications for aging and cancer. RN homologues in non-human systems"GANN MONOGRAPH ON CANCER RESEARCH. (印刷中).

[文献書誌] Kanaya, E., Nakajima, N., Morikawa, K., Okada, K., and Shimura, Y.: "Characterization of the transcription activator CBF1 from Arabidopsis thaliana"J. Biol. Chem.. 274. 16068-16076 (1999)

[文献書誌] Hanada, N., Nureki, O., Kurimoto, K., Kim, I., Sakamoto, H., Shimura, Y., Muto, Y., and Yokoyama, S.: "Structural basis for recognition of the tra mRNA precursor by the Sex-lethal protein"Nature. 398. 576-585 (1999)

[文献書誌] Sawa, S., Ito, T., Shimuka, Y., and Okada, K.: "FIL AMENTOUS FLOWER controls the formation and development of Arabidopsis inflorescences and floral meristems"Plant Cell. 11. 69-86 (1999)

[文献書誌] Fujii, Y., Shimokawa, H., Sekiguchi, M., and Nakabeppu, Y.: "Functional significance of the conserved residues for the 23-residue module among MTH1 and MutT family proteins"J. Biol. Chem.. 274. 38251-38259 (1999)

[文献書誌] Oda, H., Taketomi, A., Maruyama, R., Itoh, R., Nishioka, K., Yakushiji, H., Suzuki, T., Sekiguchi, M., and Nakabeppu, Y.: "Multiforms of human MTH1 polypeptides produced by alternative translation initiation and single nucleotide polymorphism"Nucleic Acids Res.. 27. 4335-4343 (1999)

[文献書誌] Sekiguchi, M., and Sanada, M.: "Alkylation carcinogenesis in mice with altered levels of DNA repair methyltransferase"Prog. Exp. Tumor Res.. 35. 25-36 (1999)

[文献書誌] Hayakawa, H., Hofer, A., Thelander, L., Kitajima, S., Cai, Y., Oshiro, S., Yakushiji, H., Nakebeppu, Y., Kuwano, M., and Sekiguchi, M.: "Metabolic fate of exidized guanine ribonucleotides in mammalian cells"Biochemistry. 38. 3610-3614 (1999)

[文献書誌] Nakagawa, T. and Ogawa, H.: "The Saccharomyces cerevisiae MER3 gene, encoding a novel helicase-like protein, is required for crossover control in meiosis"EMBO J.. 18. 5714-5723 (1999)

[文献書誌] Shiga, Y., Sekine, Y., and Ohtsubo, E.: "Transposition of IS1 circles"Genes Cells. 4. 551-561 (1999)

[文献書誌] Sekine, Y., Aihara, K., and Ohtsubo, E.: "Linearization and transposistion of circular molecules of insertion sequence IS3"J. Mol. Biol.. 294. 21-34 (1999)

[文献書誌] Noma, K., Ohtsubo, E., and Ohtsubo, H.: "Non-LTR retrotransposons (LINEs) as ubiquitous components of plant genomes"Mol. Gen. Genet.. 261. 71-79 (1999)

[文献書誌] Kumekawa, N., Ohtsubo, H., Horiuchi., T., and Ohtsubo, E.: "Identification and characterization of novel retrotransposons of the gypsy type in rice"Mol. Gen. Genet.. 260. 593-602 (1999)

[文献書誌] Hasebe, A. and Iida, S.: "The novel insertion sequences IS1417, IS1418 and IS1419 from Burkholderia glumae and their strain distribution"Plasmid. (印刷中).

[文献書誌] Iida, S., Hoshino, A., Johzuka-Hisatomi, Y., Habu Y. and Inagaki, Y.: "Floricultural traits and transposable elements in the Japanese and common morning glories"Annal. New York Acad. Sci.. 870. 265-274 (1999)

[文献書誌] Iida S. and Hoshino, A.: "Spontaneous mutagenesis and transposable elements in the Japanese morning glory"Gamma Field Symposia. (印刷中).

[文献書誌] Inagaki, Y., Johzuka-Hisatomi, Y., Mori, T., Takahashi, S., Hayakawa, Y., Peyachoknagul, S., Ozeki Y. and IIda, S.: "Genomic organization of the genes encoding dihydroflavonol 4-reductase for flower pigmentation in the Japanese and common morning glories"Gene. 226. 181-188 (1999)

[文献書誌] Johzuka-Hisatomi, Y., Hoshino, A., Mori, T., Habu Y. and IIda, S.: "Characterization of the chalcone synthase genes expressed in flowers of the common and Japanese morning glories"Genes Genet. Syst.. 74. 141-147 (1999)

[文献書誌] Shiokawa, K., Inagaki, Y., Morita, H., Hsu, T.-J., Iida S. and Noguchi,H.: "The functional expression of the CHS-D and CHS-E genes of the common morning glory (Ipomoea purpurea) in Escherichia coli and characterization of their gene products"Plant Biotechnology. (印刷中).

1999 年度実績報告書

池田日出男 (社)北里研究所, 基礎研究所, 部長 (10012775)