2001 年度実績報告書

活性酸素種の生成・検出・反応制御に関する電気化学的アプローチ

研究課題

研究課題/領域番号	10305064
研究機関	東京工業大学
研究代表者	大坂武男東京工業大学, 大学院・総合理工学研究科, 教授 (80152099)
研究分担者	岡島武義東京工業大学, 大学院・総合理工学研究科, 助手 (70194347) 北村房男東京工業大学, 大学院・総合理工学研究科, 助教授 (00224973)
キーワード	活性酵素種 / スーパーオキシドイオン / バイオセンサ / 過酸化水素 / 酵素 / 電気化学 / 自己組織化単分子膜 / スーパーオキシドジスムターゼ
研究概要	本研究では、様々な生命現象に密接に関連している活性酸素種(スーパーオキシドイオン、過酸化水素、一酸化窒素など)の電解生成、その場検出、反応制御、反応特異性の解明を目的として行われ、本年度は次の成果を得た。 1.電子移動プロモターの自己組織化単分子膜修飾電極に、さらにスーパーオキシドジスムターゼ(SOD)酵素を配向・固定した"SOD電極"を構築し、スーパーオキシドイオンの検出に適用し、第3世代のスパーオキシドイオンバイオセンサーの開発に成功した。 2.ポリアニリン及びポリアニリンスルホン酸修飾電極を用いて、スーパーオキシドイオンの電解生成に成功し、その生成反応機構を明らかにした。 3.自己組織化単分子膜修飾電極を用いて、生体内の代表的な抗酸化物質であるアスコルビン酸、尿酸、神経伝達物質およびその代謝物の同時分析・定量に成功した。 4.過酢酸と過酸化水素の電気化学的同時検出法を確立した。

研究成果
(29件)

すべてその他

すべて文献書誌 (29件)

[文献書誌] M. S. SAHA: "Current Oscillation Behavior of O_2/O_2￣Redox Couple at HMDE in Non-aquecus Aprotic Media"J. Electroanal. Chem.. 496・1〜2. 61-68 (2001)
[文献書誌] K. ARIHARA: "Characterization of the Adsorption State of Carboanate Ions at the Au(111) Electrode Surface…"J. Electroanal. Chem.. 510. 128-135 (2001)
[文献書誌] C. R. RAJ: "Electroanalysis of Ascorbate and Dopamine at Gold Electrode Modified with Positively charged"J. Electroanal. Chem.. 496・1〜2. 44-49 (2001)
[文献書誌] N.WAKABAYASHI: "Effect of Adsorbed Anions on the Outer-Sphere Electron-Transfer Reactions of Cobalt Complexes…"J. Electroanal. Chem.. 499. 161-168 (2001)
[文献書誌] F.MATSUMOTO: "Electrochemical Reduction of Molecular Oxygen at Hg Adatom-Modified Au Electrode"J. Electroanal. Chem.. 505. 150-158 (2001)
[文献書誌] M.I.AWAD: "Simultaneous Electoroanalysis of Peroxyacetic Acid and Hydrogen Peroxide"Anal. chem.. 73・8. 1839-1843 (2001)
[文献書誌] C. R.RAJ: "facilitated Electrochemical Oxidation of NADH and its Model Compound at Gold Electrode"Bioelectrochemistry. 53. 251-256 (2001)
[文献書誌] C. R.RAJ: "Electroanatytical Applications of Cationic Self-assembled Monolayers"Bioelectrochemistry. 53. 183-191 (2001)
[文献書誌] 上杉慎治: "水銀アドアトム修飾電極上での過酸化水素の還元反応における電気化学振動現象"Electrochemistry. 69・9. 681-685 (2001)
[文献書誌] C. R.RAJ: "Simultaneous Electroanalysys of Ascorbic Acid, Dihydroxyphenylacetic Acid, Homovanilic Acid…"Chem.Lett.. 670-671 (2001)
[文献書誌] MS. S. AWAD: "Electroanalysis of Peracetic Acid in the Presence of a Large Excess of Hydrogen Peroxide"Anal.Lett.. 34・7. 1215-1221 (2001)
[文献書誌] C. R.RAJ: "Electrocatalytic sensing of NADH at an in situ Functionalized Self-assembled Monalayer on…"Electrochem. Commun.. 3. 633-638 (2001)
[文献書誌] C. R.RAJ: "Electrochemical and in situ FTIR Spectroscopic Investigation on the Electrochemical Transformation…"Langmuir. 17. 7378-7386 (2001)
[文献書誌] H.ONO: "Electrogeneration of Superoxide Ion at Polyaniline-modified Electrodes in Aqueous Media"Electrochemistry. 69・12. 953-955 (2001)
[文献書誌] K.ARIHARA: "In situ Monitoring of the Mercury Electrode Surface in HClO_4 Slutions by Means of Infrared"J. Electroanal. chem.. 518. 139-143 (2002)
[文献書誌] 大木成政: "アルカリ性水溶液中におけるポリアニリンスルホン酸修飾電極を用いたスーパーオキシドイオンの電極生成"Electrochemistry. 70・1. 23-29 (2002)
[文献書誌] C.R.RAJ: "Analytical Application of Functionalized Self-assembled Monolayers on Gold Electrodes"Electroanalysis. (発表予定). (2000)
[文献書誌] T.OHSAKA: "Superoxide Ion Sensor"Anal. Sci.. (発表予定). (2002)
[文献書誌] L.MAO.: "Electrochemical Characterization of Catalytic Activites of Manganese Oxides to Oxygen…"J. Electrochem. Soc.. 149・40(発表予定). (2002)
[文献書誌] M.S.SAHA: "Interferometric Measurement of the Current Oscillation in the Redox Reaction of the O_2/O^-_2"J. Phys. Chem.. (発表予定). (2002)
[文献書誌] Y.TIAN: "A Facilitated Electron-Transfer of Copper-Zinc superoxide Disumutase (SOD) Based on a Cysteine"Biochem. Biophys. Acta. (発表予定). (2002)
[文献書誌] T.OKAJIMA: "Chemiluminescense of Indole and Its Derivatives induced by Electrogeneradted Superoxide Ion…"Electrochem. Acta. (発表予定). (2002)
[文献書誌] M. S. El-DEAB: "An Extraordinary Electrocatalytic Reduction of Oxygen on Gold Nanoparticles-electrodeposited"Electrchem. Commun.. 4(発表予定). (2002)
[文献書誌] D.ZHANG: "Electrochemical Reduction of Dioxygen on Mercury Electrode in Alkaline Aqueous Media"Jpn. J. Deuterium Sci.. (発表予定). (2002)
[文献書誌] T.OKAJIMA: "Chemiluminescence of 3-Methylindole Based on Electrogenercution of Superoxide Ion…"J. Electroanal. Chem.. (発表予定). (2002)
[文献書誌] C.R.RAJ: "Detection of Uric Acid in the Presence of Ascorbic Acid at Gold Electrode Modified with Self-assembled…"Anal. Chem.. (発表予定). (2002)
[文献書誌] K.ARIHARA: "Characteristic Cyclic Voltammograms of Alkyl Viologens at Single Crystal Gold Electrodes"Phys. Chem. Chem. Phys.. (発表予定). (2002)
[文献書誌] 大坂武雄(分担): "界面ハンドブック"エヌ・ティー・エス. 1208 (2001)
[文献書誌] 大坂武雄(分担): "電気化学測定マニュアル"丸善(株)(電気化学会編)(発表予定). (2002)

2001 年度 実績報告書

活性酸素種の生成・検出・反応制御に関する電気化学的アプローチ

研究代表者

大坂 武男 東京工業大学, 大学院・総合理工学研究科, 教授 (80152099)

研究成果

[文献書誌] M. S. SAHA: "Current Oscillation Behavior of O_2/O_2￣Redox Couple at HMDE in Non-aquecus Aprotic Media"J. Electroanal. Chem.. 496・1〜2. 61-68 (2001)

[文献書誌] K. ARIHARA: "Characterization of the Adsorption State of Carboanate Ions at the Au(111) Electrode Surface…"J. Electroanal. Chem.. 510. 128-135 (2001)

[文献書誌] C. R. RAJ: "Electroanalysis of Ascorbate and Dopamine at Gold Electrode Modified with Positively charged"J. Electroanal. Chem.. 496・1〜2. 44-49 (2001)

[文献書誌] N.WAKABAYASHI: "Effect of Adsorbed Anions on the Outer-Sphere Electron-Transfer Reactions of Cobalt Complexes…"J. Electroanal. Chem.. 499. 161-168 (2001)

[文献書誌] F.MATSUMOTO: "Electrochemical Reduction of Molecular Oxygen at Hg Adatom-Modified Au Electrode"J. Electroanal. Chem.. 505. 150-158 (2001)

[文献書誌] M.I.AWAD: "Simultaneous Electoroanalysis of Peroxyacetic Acid and Hydrogen Peroxide"Anal. chem.. 73・8. 1839-1843 (2001)

[文献書誌] C. R.RAJ: "facilitated Electrochemical Oxidation of NADH and its Model Compound at Gold Electrode"Bioelectrochemistry. 53. 251-256 (2001)

[文献書誌] C. R.RAJ: "Electroanatytical Applications of Cationic Self-assembled Monolayers"Bioelectrochemistry. 53. 183-191 (2001)

[文献書誌] 上杉慎治: "水銀アドアトム修飾電極上での過酸化水素の還元反応における電気化学振動現象"Electrochemistry. 69・9. 681-685 (2001)

[文献書誌] C. R.RAJ: "Simultaneous Electroanalysys of Ascorbic Acid, Dihydroxyphenylacetic Acid, Homovanilic Acid…"Chem.Lett.. 670-671 (2001)

[文献書誌] MS. S. AWAD: "Electroanalysis of Peracetic Acid in the Presence of a Large Excess of Hydrogen Peroxide"Anal.Lett.. 34・7. 1215-1221 (2001)

[文献書誌] C. R.RAJ: "Electrocatalytic sensing of NADH at an in situ Functionalized Self-assembled Monalayer on…"Electrochem. Commun.. 3. 633-638 (2001)

[文献書誌] C. R.RAJ: "Electrochemical and in situ FTIR Spectroscopic Investigation on the Electrochemical Transformation…"Langmuir. 17. 7378-7386 (2001)

[文献書誌] H.ONO: "Electrogeneration of Superoxide Ion at Polyaniline-modified Electrodes in Aqueous Media"Electrochemistry. 69・12. 953-955 (2001)

[文献書誌] K.ARIHARA: "In situ Monitoring of the Mercury Electrode Surface in HClO_4 Slutions by Means of Infrared"J. Electroanal. chem.. 518. 139-143 (2002)

[文献書誌] 大木成政: "アルカリ性水溶液中におけるポリアニリンスルホン酸修飾電極を用いたスーパーオキシドイオンの電極生成"Electrochemistry. 70・1. 23-29 (2002)

[文献書誌] C.R.RAJ: "Analytical Application of Functionalized Self-assembled Monolayers on Gold Electrodes"Electroanalysis. (発表予定). (2000)

[文献書誌] T.OHSAKA: "Superoxide Ion Sensor"Anal. Sci.. (発表予定). (2002)

[文献書誌] L.MAO.: "Electrochemical Characterization of Catalytic Activites of Manganese Oxides to Oxygen…"J. Electrochem. Soc.. 149・40(発表予定). (2002)

[文献書誌] M.S.SAHA: "Interferometric Measurement of the Current Oscillation in the Redox Reaction of the O_2/O^-_2"J. Phys. Chem.. (発表予定). (2002)

[文献書誌] Y.TIAN: "A Facilitated Electron-Transfer of Copper-Zinc superoxide Disumutase (SOD) Based on a Cysteine"Biochem. Biophys. Acta. (発表予定). (2002)

[文献書誌] T.OKAJIMA: "Chemiluminescense of Indole and Its Derivatives induced by Electrogeneradted Superoxide Ion…"Electrochem. Acta. (発表予定). (2002)

[文献書誌] M. S. El-DEAB: "An Extraordinary Electrocatalytic Reduction of Oxygen on Gold Nanoparticles-electrodeposited"Electrchem. Commun.. 4(発表予定). (2002)

[文献書誌] D.ZHANG: "Electrochemical Reduction of Dioxygen on Mercury Electrode in Alkaline Aqueous Media"Jpn. J. Deuterium Sci.. (発表予定). (2002)

[文献書誌] T.OKAJIMA: "Chemiluminescence of 3-Methylindole Based on Electrogenercution of Superoxide Ion…"J. Electroanal. Chem.. (発表予定). (2002)

[文献書誌] C.R.RAJ: "Detection of Uric Acid in the Presence of Ascorbic Acid at Gold Electrode Modified with Self-assembled…"Anal. Chem.. (発表予定). (2002)

[文献書誌] K.ARIHARA: "Characteristic Cyclic Voltammograms of Alkyl Viologens at Single Crystal Gold Electrodes"Phys. Chem. Chem. Phys.. (発表予定). (2002)

[文献書誌] 大坂武雄(分担): "界面ハンドブック"エヌ・ティー・エス. 1208 (2001)

[文献書誌] 大坂武雄(分担): "電気化学測定マニュアル"丸善(株)(電気化学会編)(発表予定). (2002)

2001 年度実績報告書

大坂武男東京工業大学, 大学院・総合理工学研究科, 教授 (80152099)