1999 年度実績報告書

免疫不全マウスを用いた人体寄生テニア科条虫感染病態(有鉤襄虫症)モデルの開発

研究課題

研究課題/領域番号	10480235
研究機関	旭川医科大学
研究代表者	伊藤亮旭川医科大学, 医学部・寄生虫学, 教授 (70054020)
研究分担者	迫康仁旭川医科大学, 医学部・寄生虫学, 助手 (40312459) 中尾稔旭川医科大学, 医学部・寄生虫学, 助手 (70155670) 伊藤守実験動物中央研究所, 免疫研究室, 室長 (00176364) 中谷和宏旭川医科大学, 医学部・動物実験施設, 教務職員 (70109388)
キーワード	免疫不全マウス / 有鉤襄虫症 / エキノコックス症 / 病態モデル動物 / テニア科条虫 / 無鉤条虫 / 有鉤条虫
研究概要	新興・再興感染症として世界的規模で流行、汚染地域拡大が年々深刻化してきている寄生虫病にテニア科条虫の幼虫が人体寄生することによって惹起される襄虫症、包虫症がある。無鉤条虫(Taenia saginata)、有鉤条虫(T.solium)、それに東南アジアを中心に問題になっているAsian Taenia(T.asiatica)を含め、一応3種類(T.saginata, T.asiatica, T.solium)がヒトを終宿主とするテニア科条虫として知られている。これら3種類の条虫は「中間宿主としてウシ、ブタなどの大型家畜動物あるいはヒトを必要とし、この中間宿主寄生が非常に重篤な病害を与える」こと、また「終宿主がヒトであるため、患者から虫卵を入手しなければならない」ことから、研究が非常に困難である。本研究では家畜ならびにヒトの代わりに実験動物を中間宿主動物として利用する、疾患モデル動物作製が目的である。本研究では世界各国から入手できたテニア科条虫3種虫卵を用いた感染実験から人体寄生テニア科条虫の中間宿主実験動物モデルとしてNOD-SCIDマウスが格段に感受性が高いという成績が得られている。また最も難治性であるエキノコックス症についての病態動物モデル、治療薬検定動物モデルの作製も試みてきている。病態動物モデル作製ではマウス、ラットを用い、肝、肺、脳に病巣を実験的に作出する方法を確立している。さらにマウスを終宿主、昆虫を中間宿主とするHymenolepis microsotma の虫卵をNMRI-nude マウスならびにNOD-SCID マウスに経口投与することによりマウス体内では絶対に幼虫の発育は起こらないとされていた定説を覆す新しい発見があった。以上の世界で最初の成績により、実験解析が可能な時代になったと総括される。

研究成果

(23件)

すべてその他

すべて文献書誌 (23件)

[文献書誌] Ito A.,Nakao M.,Sako Y.,Nakaya K.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health. (in press). (2000)
[文献書誌] Wandra T.,Subahar R.,Simaniuntak GM,,Margono SS.,Suroso T,Okamoto M.,Nakao M.,Sako Y.,Nakaya K.,Schantz PM.,Ito A.: "Resurgence of cases of epileptic seizures and burns associated with cysticercosis in Assologaima, Jayawijaya, Irian Jaya, Indonesia, 1991-95"Transactions of the Royal Society of Tropical Medicine and Hygiene. 94. 1-5 (2000)
[文献書誌] Ito A.,Plancarte A.,Nakao M.,Nakaya K.,Ikejima T.,Piao ZX.,Kanazawa T.,Margono SS.: "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium"Journal of Helminthology. 73. 363-365 (1999)
[文献書誌] Qiu JM.,Liu FJ.,Wang H..,Ito A.,Schantz PM.: "A survey of hydatid disease (echinococcosis) in Tibetan populations in China : Correlation of ultrasound and radiologic imaging and serologic results"International Archives of Hydatidosis. 33. 211-213 (1999)
[文献書誌] Ito A.: "Differential serodiagnosis for larval cestode infections : cystic ehinococcosis, alveolar echinococcosis and neurocysticercosis"International Archives of Hydatidosis. 33. 166-170 (1999)
[文献書誌] Kimura M.,Nakamura T.,Iwamoto A.,Nishimura Y.,Egawa T.,Ito A.: "Cystic echinococcosis in a Jordanian patient : albendazole in a short-term immigrant"Journal of Travel Medicine. 6. 249-253 (1999)
[文献書誌] Wang IC.,Ma YX.,Guo JX.,Chung WC.,Lu SC.,Ito A.,Fan PC.: "Oncospheres of Taenia solium and T. saginata asiatica develop into metacestodes in normal and immunosuppressed mice"Journal of Helminthology. 73. 183-186 (1999)
[文献書誌] Willingham III AL.,Johansen MV.,Bogh HO.,Ito A.,Andreassen J.,Lindberg R.,Christensen NO.,Nansen P.: "Congenital transmission of Schistosoma japonicum in pigs"American Journal of Tropical Medicine and Hygiene. 60. 311-312 (1999)
[文献書誌] Ito A.,Ito M.: "Human Taenia in Severe Combined Immunodeficiency (SCID) mice"Parasitology Today. 15. 64-67 (1999)
[文献書誌] Ohsaki Y.,Matsumoto A.,Miyamoto K.,Kondoh N.,Araki K.,Ito A.,Kikuchi K.: "Neurocysticercosis without detectable specific antibody"Ineternal Medicine. 38. 67-70 (1999)
[文献書誌] Ito A.,Nakao M.,Ito Y.,Yuzawa I.,Morishima H.,Kawano N.,Fujii K.: "Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection"Parasitology International. 48. 95-99 (1999)
[文献書誌] Ito A.,Ma L.,Schantz PM.,Gottstein B.,Liu YH.,Chai JJ.,Abder-Hafez SK.,Altintas N.,Joshi DD.,Lightowlers MW.,Pawlowski ZS.: "Differential serodiagnosis for cystic and alveolar echinococcosis using fractions of Echinococcus granulosus cyst fluid (antigen B) and E. multilocularis protoscolex (Em18)"American Journal of Tropical Medicine and Hygiene. 60. 188-192 (1999)
[文献書誌] Ito A.,Plancarte A.,Ma L.,Kong Y.Flisser A.,Cho YS.,Liu YH.,Kamhawi S.,Lightowlers MW.,Schantz PM.: "Novel antigens for neurocysticercosis : simple method for preparation and evaluation for serodiagnosis"American Journal of Tropical Medicine and Hygiene. 59. 291-294 (1998)
[文献書誌] Niwa A.,Asano K.,Ito A.: "Eosinophil chemotactic factors from cysticercoids of Hymenolepis nana"Journal of Helminthology. 20. 483-488 (1998)
[文献書誌] Yang HJ, Chung JY, Yun DH, Kong Y, Ito A, Ma L, Liu YH, Lee SC, Kang SY, Cho SY: "Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes"Parasite Immunology. 20. 483-488 (1998)
[文献書誌] Kong Y.,Ito A.,Yang HJ.,Chung YB.,Kasuya S.,Kobayashi M.,Liu YH.,Cho SY.: "Immunoglobulin G (IgG) subclass and IgE responses in human paragonimiases caused by three different species"Clinical and Diagnostic Laboratory Immunology. 5. 474-478 (1998)
[文献書誌] Ito A.,Ma L.,Paul M.,Stefaniak J.,Pawlowski ZS.: "Evaluatioan of Em18-, Em16-, Antigen B-Western blots, Em2^<plus>-ELISA and four other tests for differential serodiagnosis of alveolar and cystic echinococcosis patients in Poland"Parasitology International. 47. 95-99 (1998)
[文献書誌] Ito A.,Okamoto M.,Ishiguro T.,Ma L., Suzuki H.,Yasui A.,Ahigeta H.,Matsuura T.,Hosokawa T.,Chai JJ.: "An imported case of cystic echinococcosis in Japan diagnosed by imaging and serology with confirmation of Echinococcus granulosus-specific DNA sequences"American Journal of Tropical Medicine and Hygiene. 58. 790-792 (1998)
[文献書誌] Ito A.,Sakakibara Y.,Ma L.,Asano K.,Takiguchi M.,Yasuda J.,Hashimoto A.: "Ultrasonographic and serologic studies of experimental cysticercosis in rats infected with Taenia taeniaeformis"Parasite Immunology. 20. 105-110 (1998)
[文献書誌] Nakaya K.,Oomori Y.,Kutsumi H.,Nakao M.: "Morphological changes of larval Echinococcus multilocularis in mice treated with albendazole or mebendazole"Journal of Helminthology. 72. 349-354 (1998)
[文献書誌] Sato Y.,Nakao M.,Nakaya K.,Ito A.: "Experimental infecition of larval Echinococcus multilocularis in the rodent brain as a model for cerebral alveolar echinococcosis"Journal of Helminthology. 72. 59-64 (1998)
[文献書誌] 伊藤亮: "日本における寄生虫学の研究第7巻6章"目黒寄生虫館. 684 (1999)
[文献書誌] 辻守康、近藤力王至、伊藤亮: "日本における寄生虫学の研究第6巻5章"目黒寄生虫館. 672 (1999)

1999 年度 実績報告書

免疫不全マウスを用いた人体寄生テニア科条虫感染病態(有鉤襄虫症)モデルの開発

研究代表者

伊藤 亮 旭川医科大学, 医学部・寄生虫学, 教授 (70054020)

研究成果

[文献書誌] Ito A.,Nakao M.,Sako Y.,Nakaya K.: "Neurocysticercosis and echinococcosis in Asia : Recent advances in the establishment of highly reliable differential serodiagnosis for international collaboration"Southeast Asian Journal of Tropical Medicine and Public Health. (in press). (2000)

[文献書誌] Ito A.,Plancarte A.,Nakao M.,Nakaya K.,Ikejima T.,Piao ZX.,Kanazawa T.,Margono SS.: "ELISA and immunoblot using purified glycoproteins for serodiagnosis of cysticercosis in pigs naturally infected with Taenia solium"Journal of Helminthology. 73. 363-365 (1999)

[文献書誌] Qiu JM.,Liu FJ.,Wang H..,Ito A.,Schantz PM.: "A survey of hydatid disease (echinococcosis) in Tibetan populations in China : Correlation of ultrasound and radiologic imaging and serologic results"International Archives of Hydatidosis. 33. 211-213 (1999)

[文献書誌] Ito A.: "Differential serodiagnosis for larval cestode infections : cystic ehinococcosis, alveolar echinococcosis and neurocysticercosis"International Archives of Hydatidosis. 33. 166-170 (1999)

[文献書誌] Kimura M.,Nakamura T.,Iwamoto A.,Nishimura Y.,Egawa T.,Ito A.: "Cystic echinococcosis in a Jordanian patient : albendazole in a short-term immigrant"Journal of Travel Medicine. 6. 249-253 (1999)

[文献書誌] Wang IC.,Ma YX.,Guo JX.,Chung WC.,Lu SC.,Ito A.,Fan PC.: "Oncospheres of Taenia solium and T. saginata asiatica develop into metacestodes in normal and immunosuppressed mice"Journal of Helminthology. 73. 183-186 (1999)

[文献書誌] Willingham III AL.,Johansen MV.,Bogh HO.,Ito A.,Andreassen J.,Lindberg R.,Christensen NO.,Nansen P.: "Congenital transmission of Schistosoma japonicum in pigs"American Journal of Tropical Medicine and Hygiene. 60. 311-312 (1999)

[文献書誌] Ito A.,Ito M.: "Human Taenia in Severe Combined Immunodeficiency (SCID) mice"Parasitology Today. 15. 64-67 (1999)

[文献書誌] Ohsaki Y.,Matsumoto A.,Miyamoto K.,Kondoh N.,Araki K.,Ito A.,Kikuchi K.: "Neurocysticercosis without detectable specific antibody"Ineternal Medicine. 38. 67-70 (1999)

[文献書誌] Ito A.,Nakao M.,Ito Y.,Yuzawa I.,Morishima H.,Kawano N.,Fujii K.: "Neurocysticercosis case with a single cyst in the brain showing dramatic drop in specific antibody titers within 1 year after curative surgical resection"Parasitology International. 48. 95-99 (1999)

[文献書誌] Ito A.,Plancarte A.,Ma L.,Kong Y.Flisser A.,Cho YS.,Liu YH.,Kamhawi S.,Lightowlers MW.,Schantz PM.: "Novel antigens for neurocysticercosis : simple method for preparation and evaluation for serodiagnosis"American Journal of Tropical Medicine and Hygiene. 59. 291-294 (1998)

[文献書誌] Niwa A.,Asano K.,Ito A.: "Eosinophil chemotactic factors from cysticercoids of Hymenolepis nana"Journal of Helminthology. 20. 483-488 (1998)

[文献書誌] Yang HJ, Chung JY, Yun DH, Kong Y, Ito A, Ma L, Liu YH, Lee SC, Kang SY, Cho SY: "Immunoblot analysis of a 10 kDa antigen in cyst fluid of Taenia solium metacestodes"Parasite Immunology. 20. 483-488 (1998)

[文献書誌] Kong Y.,Ito A.,Yang HJ.,Chung YB.,Kasuya S.,Kobayashi M.,Liu YH.,Cho SY.: "Immunoglobulin G (IgG) subclass and IgE responses in human paragonimiases caused by three different species"Clinical and Diagnostic Laboratory Immunology. 5. 474-478 (1998)

[文献書誌] Ito A.,Ma L.,Paul M.,Stefaniak J.,Pawlowski ZS.: "Evaluatioan of Em18-, Em16-, Antigen B-Western blots, Em2^<plus>-ELISA and four other tests for differential serodiagnosis of alveolar and cystic echinococcosis patients in Poland"Parasitology International. 47. 95-99 (1998)

[文献書誌] Ito A.,Sakakibara Y.,Ma L.,Asano K.,Takiguchi M.,Yasuda J.,Hashimoto A.: "Ultrasonographic and serologic studies of experimental cysticercosis in rats infected with Taenia taeniaeformis"Parasite Immunology. 20. 105-110 (1998)

[文献書誌] Nakaya K.,Oomori Y.,Kutsumi H.,Nakao M.: "Morphological changes of larval Echinococcus multilocularis in mice treated with albendazole or mebendazole"Journal of Helminthology. 72. 349-354 (1998)

[文献書誌] Sato Y.,Nakao M.,Nakaya K.,Ito A.: "Experimental infecition of larval Echinococcus multilocularis in the rodent brain as a model for cerebral alveolar echinococcosis"Journal of Helminthology. 72. 59-64 (1998)

[文献書誌] 伊藤 亮: "日本における寄生虫学の研究 第7巻6章"目黒寄生虫館. 684 (1999)

[文献書誌] 辻守康、近藤力王至、伊藤 亮: "日本における寄生虫学の研究 第6巻5章"目黒寄生虫館. 672 (1999)

1999 年度実績報告書

伊藤亮旭川医科大学, 医学部・寄生虫学, 教授 (70054020)

[文献書誌] 伊藤亮: "日本における寄生虫学の研究第7巻6章"目黒寄生虫館. 684 (1999)

[文献書誌] 辻守康、近藤力王至、伊藤亮: "日本における寄生虫学の研究第6巻5章"目黒寄生虫館. 672 (1999)