1999 年度実績報告書

血中酸化LDL定量法による新しい体外診断薬への展開

研究課題

研究課題/領域番号	10557225
研究機関	帝京大学
研究代表者	高野達哉帝京大学, 薬学部, 教授 (40124995)
研究分担者	東祐輔帝京大学, 薬学部, 助手 (60286979) 森雅博帝京大学, 薬学部, 助手 (00230079) 板部洋之帝京大学, 薬学部, 講師 (30203079)
キーワード	動脈硬化 / 腎疾患 / 体外診断薬 / 酸化リポタンパク質 / 過酸化脂質
研究概要	われわれは、酸化LDLをより定量的に評価することを目的として、DLH3抗体を利用した酸化LDLの高感度定量法を開発した。この方法で、硫酸銅処理した酸化LDLを標準物質とすると、酸化LDLの蛋白量で10mg程度まで直線的に測定できる。多数の健常者、およびいくつかの疾患患者についての測定結果をまとめてみると、健常者では、LDL5μgあたり0-52ngの硫酸銅処理酸化LDLと同等の反応性が得られた。一方、人工透析患者では約8倍、家族性LCAT欠損症では、健常者の約3.5倍と有意に高い値が得られた。しかしながら、血漿総コレステロール値が有意に高い高脂血症患者ではLDL酸化値の上昇は認められない。さらに、約100名の循環器疾患患者、人工透析患者の血漿LDL酸化値を測定したところ、これら疾患患者の約半数以上は高値を示し、なかには著しく高い値を示す場合があった。すなわち、酸化LDL値はコレステロールとは独立した循環器疾患の危険因子である可能性が考えられる。

研究成果
(16件)

すべてその他

すべて文献書誌 (16件)

[文献書誌] Itabe,H.: "Detection of Oxidized Phosphatidylcholines in Human Atherosclerotic Lesions and in Plasma LDL Fractions."Pathophysiology of Lipid Peroxides and Related Free Radicals. 13-24 (1998)
[文献書誌] Itabe,H.: "Appearance of Cross Linked Proteins in Human Atheroma and Rat Pre-fibrotic Liver Detected by a New Monoclonal Antibody"Biochimica et Biophysica Acta. 1406. 28-39 (1998)
[文献書誌] Takahashi,Y.: "Spatial Pattern of Smooth Muscle Differentiation Is Specified by the Epithelium in the Stomach of Mouse Embryo."Developmental Dynamics. 212. 448-460 (1998)
[文献書誌] Tanabe,S.: "Presence of Oxidized LDL in Transplant Arteriosclerotic Lesions Obtained from Renal Transplant Recipients with Hypercholesterolemia"Transplantation Proceedings. 30. 116-118 (1998)
[文献書誌] Itabe,H.: "Oxidized Phospholipids as a New Landmark in Atherosclerosis."Progress in Lipid Research. 37(2/3). 181-207 (1998)
[文献書誌] Kawabe,Y.: "Characterization of Two Chinese Hamster Ovary Cell Lines Expressing the COOH-terminal Domains of Sterol Regulatory Element-Binding Protein(SREBP)-1."Cell Structure and Function. 23. 187-192 (1998)
[文献書誌] Jimi,S.: "Deposition of Oxidized Low-Density Lipoprotein and Collagenosis Occur Coincidentally in Humen Coronary Stenosis:an Immunohistochemical Study of Atherectomy."Coronary Artery Disease. 9(9). 551-557 (1998)
[文献書誌] Sakata,N.: "Immunohistochemical Localization of Different Epitopes of Advanced Glycation End Products in Human Atherosclerotic Lesions."Atherosclerosis. 141. 61-75 (1998)
[文献書誌] Jimi,S.: "Possible Induction of Renal Dysfunction in Patients with Lecithin:Cholesterol Acyltransferase Deficiency by Oxidized Phosphatidylcholine in Glomeruli."Arteriosclerosis Thrombosis Vascular Biology. 19. 794-801 (1999)
[文献書誌] Imanaka,T.: "Characterization of the 70-kDa Peroxisomal Membrane Protein,an ATP Binding Cassette Transporter"The Journal of Biological Chemistry. 274(17). 11968-11976 (1999)
[文献書誌] Itabe,H.: "Metabolism of Oxidized Phosphatidylcholines Formed in Oxidized Low Density Lipoprotein by Lecithin-Cholesterol Acyltransferase"Journal of Biochemistry. 126(1). 153-161 (1999)
[文献書誌] Mori,M.: "Presence of Phospholipid-Neutral Lipid Complex Structures in Atherosclerotic Lesions as Detected by a Novel Monoclonal Antibody"The Journal of Biological Chemistry. 274(35). 24828-24837 (1999)
[文献書誌] 高野: "腎臓21(3)"動脈硬化と過酸化脂質そして腎疾患へ. 6 (1998)
[文献書誌] 板部: "動脈硬化26(1)"酸化LDLの修飾構造と代謝につい. 5 (1998)
[文献書誌] 板部: "治療学32(4)"酸化LDLの測定. 4 (1998)
[文献書誌] 板部: "The Lipid 10(3)"リポ蛋白「酸化」の生化学とリポ蛋白代謝. 6 (1999)

1999 年度 実績報告書

血中酸化LDL定量法による新しい体外診断薬への展開

研究代表者

高野 達哉 帝京大学, 薬学部, 教授 (40124995)

研究成果

[文献書誌] Itabe,H.: "Detection of Oxidized Phosphatidylcholines in Human Atherosclerotic Lesions and in Plasma LDL Fractions."Pathophysiology of Lipid Peroxides and Related Free Radicals. 13-24 (1998)

[文献書誌] Itabe,H.: "Appearance of Cross Linked Proteins in Human Atheroma and Rat Pre-fibrotic Liver Detected by a New Monoclonal Antibody"Biochimica et Biophysica Acta. 1406. 28-39 (1998)

[文献書誌] Takahashi,Y.: "Spatial Pattern of Smooth Muscle Differentiation Is Specified by the Epithelium in the Stomach of Mouse Embryo."Developmental Dynamics. 212. 448-460 (1998)

[文献書誌] Tanabe,S.: "Presence of Oxidized LDL in Transplant Arteriosclerotic Lesions Obtained from Renal Transplant Recipients with Hypercholesterolemia"Transplantation Proceedings. 30. 116-118 (1998)

[文献書誌] Itabe,H.: "Oxidized Phospholipids as a New Landmark in Atherosclerosis."Progress in Lipid Research. 37(2/3). 181-207 (1998)

[文献書誌] Kawabe,Y.: "Characterization of Two Chinese Hamster Ovary Cell Lines Expressing the COOH-terminal Domains of Sterol Regulatory Element-Binding Protein(SREBP)-1."Cell Structure and Function. 23. 187-192 (1998)

[文献書誌] Jimi,S.: "Deposition of Oxidized Low-Density Lipoprotein and Collagenosis Occur Coincidentally in Humen Coronary Stenosis:an Immunohistochemical Study of Atherectomy."Coronary Artery Disease. 9(9). 551-557 (1998)

[文献書誌] Sakata,N.: "Immunohistochemical Localization of Different Epitopes of Advanced Glycation End Products in Human Atherosclerotic Lesions."Atherosclerosis. 141. 61-75 (1998)

[文献書誌] Jimi,S.: "Possible Induction of Renal Dysfunction in Patients with Lecithin:Cholesterol Acyltransferase Deficiency by Oxidized Phosphatidylcholine in Glomeruli."Arteriosclerosis Thrombosis Vascular Biology. 19. 794-801 (1999)

[文献書誌] Imanaka,T.: "Characterization of the 70-kDa Peroxisomal Membrane Protein,an ATP Binding Cassette Transporter"The Journal of Biological Chemistry. 274(17). 11968-11976 (1999)

[文献書誌] Itabe,H.: "Metabolism of Oxidized Phosphatidylcholines Formed in Oxidized Low Density Lipoprotein by Lecithin-Cholesterol Acyltransferase"Journal of Biochemistry. 126(1). 153-161 (1999)

[文献書誌] Mori,M.: "Presence of Phospholipid-Neutral Lipid Complex Structures in Atherosclerotic Lesions as Detected by a Novel Monoclonal Antibody"The Journal of Biological Chemistry. 274(35). 24828-24837 (1999)

[文献書誌] 高野: "腎臓21(3)"動脈硬化と過酸化脂質 そして腎疾患へ. 6 (1998)

[文献書誌] 板部: "動脈硬化26(1)"酸化LDLの修飾構造と代謝につい. 5 (1998)

[文献書誌] 板部: "治療学32(4)"酸化LDLの測定. 4 (1998)

[文献書誌] 板部: "The Lipid 10(3)"リポ蛋白「酸化」の生化学とリポ蛋白代謝. 6 (1999)

1999 年度実績報告書

高野達哉帝京大学, 薬学部, 教授 (40124995)

[文献書誌] 高野: "腎臓21(3)"動脈硬化と過酸化脂質そして腎疾患へ. 6 (1998)