1999 年度実績報告書

硫酸化多糖による蛇毒中和効果の証明

研究課題

研究課題/領域番号	11121223
研究機関	鳥取大学
研究代表者	南三郎鳥取大学, 農学部, 教授 (70032307)
キーワード	硫酸化多糖 / カードラン / 毒蛇中和効果 / 生体防御 / 補体活性 / 皮下出血 / ガラガラヘビ / 兎
研究概要	硫酸カードランによる補体活性の阻止効果犬血清中のC3を活性の指標として、放射一元免疫拡散法によって判定した。補体の活性剤としてはキトサンおよびガラガラヘビ毒(Crotalus V.Viridis,Sigma,USA)を10mg/mlの濃度で用いた。硫酸カードラン(味の素、東京)の分子量は79+/-0.6kDaで、硫酸化度は15.2+/-0.3%である。硫酸カードランはキトサンおよびガラガラヘビ毒による補体の活性を完全にブロックした。毒蛇による皮下出血の阻止効果出血阻止効果実験は兎を用い、毒の投与量を0.02mgとしてCRDSを0.01,0.02,0.04,0.08,0.16,0.32mgまで変化させた。その結果、CRDSの量が毒蛇の1/2および等量の場合でも明らかな出血阻止効果が認められた。CRDSが2倍以上の例では同部位に時間をおいてCRDSを投入し、その効果を検討した。皮膚区分は頭側の2カ所が毒のみ、尾側の2カ所をCRDSのみのコントロ-ルとした。安楽死を毒投与後4時間目に実施し、出血の有無を確認した。その結果、30分後および1時間後にCRDSを投与したNO.5では出血は見とめられるものの、コントロールと比較して明瞭な阻止効果が認められた。以上の検討によってカードラン硫酸はキトサンおよび毒蛇による補対の活性作用をブロックする機能があることを証明し、ヘビの咬傷の治療薬として極めて期待できる物質であることを明らかにした。

研究成果

(31件)

すべてその他

すべて文献書誌 (31件)

[文献書誌] Yoshinori TANAKA: "Effects chitin and chitosan particles on BALB/c mice by oral parenteral administration"Biomaterials. 15. 947-951 (1997)
[文献書誌] 南三郎: "乳汁食細胞に及ぼすキトサン乳槽内投与の効果"日獣会誌. 50. 143-146 (1997)
[文献書誌] Yoshiharu OKAMOTO: "Effect of chitin on nonwoven fabric implant in tendon healing"Carbhydr.Polym.. 33. 33-38 (1997)
[文献書誌] Hiroyuki SAIMOTO: "Bioderadation of chitin with enzymes and vital components"Macromol.Symp.. 120. 11-18 (1997)
[文献書誌] Saburo MINAMI: "Systemic effect of chitin by intravenous administration in dog"Carbhydr.Polym.. 33. 243-249 (1997)
[文献書誌] Saburo MINAMI: "Subcutaneous injected shitosan induces systemic activation in dog"Carbhydr.Polym.. 33. 285-294 (1997)
[文献書誌] Yasuyuki USAMI: "Influence of shain length of N-acetyl-D-glucosamine residues direct and complement-mediated shemotactic for canine polymorphonuclear cells"Carbhydr.Polym.. 32. 115-122 (1997)
[文献書誌] 重政好弘: "キチン及びキトサンの医学領域への応用"高分子加工. 46. 75-81 (1997)
[文献書誌] 南三郎: "キチン及びその誘導体の医学・獣医学応用"獣医畜産新報. 50. 1033-1052 (1997)
[文献書誌] Saburo MINAMI: "Chitin and chitosan activate complement via the alternative pathway"Carbhydr.Polym.. 36. 151-155 (1998)
[文献書誌] Yasuyuki USAMI: "Effect of N-acetyl-D-glucosamine and D-glucosamine on canine polymorphonuclear cell in vitro"Carbhydr.Polym.. 36. 137-141 (1998)
[文献書誌] Kazuo OKAJIMA: "Collagen typing of granulation tissue induced by chitin and chitosan"Carbhydr.Polym.. 37. 109-113 (1998)
[文献書誌] Saburo MINAMI: "Bocking effect of curdlan sulfate on subcutaneous hermorrhage inducing by Crotalus V.Virdis"Abstruct of 19th ICS. Supplement. CP049 (1998)
[文献書誌] Yasuyuki USAMI: "Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2"J.Biomed.Master.Res.. 42. 517-522 (1998)
[文献書誌] Yoshiharu OKAMOTO: "Scanning electron microscopic and cytochemical analysis and peritoneal macrophages of mice,dogs and cats"J.Vet.Med.Sci.. 60. 1145-1148 (1997)
[文献書誌] 南三郎: "動物繊維キチンおよびその誘導体の生体活性効果と動物臨床応用"Sen'I Gakkaishi. 54. 266-271 (1998)
[文献書誌] Hitoshi SASHIWA: "Antimicrobial and wound healing activity of partially deacetylated chitin-isodine suspension"Chitin and Chitosan Research. 4. 18-24 (1998)
[文献書誌] Katsuyuki MIYATAKE: "Conputerized digital image processing on radiographs of canine filariosis"J.Vet.Med.Sci.. 61. 245-250 (1999)
[文献書誌] 南三郎: "キチン質の創傷癒促進進機構"チキン・キトサン研究. 5. 1-13 (1999)
[文献書誌] Y.CHOI: "Molecular structure of canine LINE-1 elements in canine transmissible venereal tumor"Animal Genetics. 30. 51-53 (1999)
[文献書誌] Xuebing LI: "Synthesis of Chitosan-Sugar Hybrid and Evaluation of its Bioactivity"Polymers for Advanced Technologies. 10. 455-458 (1999)
[文献書誌] Y.Suzuki: "Influence of physico-chemical properties of chitin and chitosan on complement activation"Carbhydr.Polym.. (in printing). (1999)
[文献書誌] Saburo MINAMI: "Drug delivery effects of oxytetracycline-hydrochloride/chitin tablet on dog"Chitin and Chitosan Research. 5. 271-278 (1999)
[文献書誌] Saburo MINAMI: "Veterinary practice with chitin and chitosan"Chitin and Chitinases. 265-277 (1999)
[文献書誌] Minoru MORIMOTO: "Synthesis of water-soluble oxidized chitosan derivatives and their biological activity"Chemistry Letters. 1285-1286 (1999)
[文献書誌] Saburo MINAMI: "Advanced in Chitin Science Vol.2"Jacques Andre Pup.,Lyon,France. 962 (1997)
[文献書誌] Yoshiharu OKAMOTO: "Advanced in Chitin Science Vol.2"Jacques Andre Pup.,Lyon,France. 962 (1997)
[文献書誌] Saburo MINAMI: "Advanced in Chitin Science Vol.3"Rita advertising Co.,Ltd. 516 (1998)
[文献書誌] Yoshiharu OKAMOTO: "Advanced in Chitin Science Vol.3"Rita advertising Co.,Ltd. 516 (1998)
[文献書誌] Saburo MINAMI: "23ed Aystralasian Polymer Symposium"Deakin University. 530 (1999)
[文献書誌] Yoshiharu OKAMOTO: "23ed Aystralasian Polymer Symposium"Deakin University. 530 (1999)

1999 年度 実績報告書

硫酸化多糖による蛇毒中和効果の証明

研究代表者

南 三郎 鳥取大学, 農学部, 教授 (70032307)

研究成果

[文献書誌] Yoshinori TANAKA: "Effects chitin and chitosan particles on BALB/c mice by oral parenteral administration"Biomaterials. 15. 947-951 (1997)

[文献書誌] 南 三郎: "乳汁食細胞に及ぼすキトサン乳槽内投与の効果"日獣会誌. 50. 143-146 (1997)

[文献書誌] Yoshiharu OKAMOTO: "Effect of chitin on nonwoven fabric implant in tendon healing"Carbhydr.Polym.. 33. 33-38 (1997)

[文献書誌] Hiroyuki SAIMOTO: "Bioderadation of chitin with enzymes and vital components"Macromol.Symp.. 120. 11-18 (1997)

[文献書誌] Saburo MINAMI: "Systemic effect of chitin by intravenous administration in dog"Carbhydr.Polym.. 33. 243-249 (1997)

[文献書誌] Saburo MINAMI: "Subcutaneous injected shitosan induces systemic activation in dog"Carbhydr.Polym.. 33. 285-294 (1997)

[文献書誌] Yasuyuki USAMI: "Influence of shain length of N-acetyl-D-glucosamine residues direct and complement-mediated shemotactic for canine polymorphonuclear cells"Carbhydr.Polym.. 32. 115-122 (1997)

[文献書誌] 重政 好弘: "キチン及びキトサンの医学領域への応用"高分子加工. 46. 75-81 (1997)

[文献書誌] 南 三郎: "キチン及びその誘導体の医学・獣医学応用"獣医畜産新報. 50. 1033-1052 (1997)

[文献書誌] Saburo MINAMI: "Chitin and chitosan activate complement via the alternative pathway"Carbhydr.Polym.. 36. 151-155 (1998)

[文献書誌] Yasuyuki USAMI: "Effect of N-acetyl-D-glucosamine and D-glucosamine on canine polymorphonuclear cell in vitro"Carbhydr.Polym.. 36. 137-141 (1998)

[文献書誌] Kazuo OKAJIMA: "Collagen typing of granulation tissue induced by chitin and chitosan"Carbhydr.Polym.. 37. 109-113 (1998)

[文献書誌] Saburo MINAMI: "Bocking effect of curdlan sulfate on subcutaneous hermorrhage inducing by Crotalus V.Virdis"Abstruct of 19th ICS. Supplement. CP049 (1998)

[文献書誌] Yasuyuki USAMI: "Chitin and chitosan stimulate canine polymorphonuclear cells to release leukotriene B4 and prostaglandin E2"J.Biomed.Master.Res.. 42. 517-522 (1998)

[文献書誌] Yoshiharu OKAMOTO: "Scanning electron microscopic and cytochemical analysis and peritoneal macrophages of mice,dogs and cats"J.Vet.Med.Sci.. 60. 1145-1148 (1997)

[文献書誌] 南 三郎: "動物繊維キチンおよびその誘導体の生体活性効果と動物臨床応用"Sen'I Gakkaishi. 54. 266-271 (1998)

[文献書誌] Hitoshi SASHIWA: "Antimicrobial and wound healing activity of partially deacetylated chitin-isodine suspension"Chitin and Chitosan Research. 4. 18-24 (1998)

[文献書誌] Katsuyuki MIYATAKE: "Conputerized digital image processing on radiographs of canine filariosis"J.Vet.Med.Sci.. 61. 245-250 (1999)

[文献書誌] 南 三郎: "キチン質の創傷癒促進進機構"チキン・キトサン研究. 5. 1-13 (1999)

[文献書誌] Y.CHOI: "Molecular structure of canine LINE-1 elements in canine transmissible venereal tumor"Animal Genetics. 30. 51-53 (1999)

[文献書誌] Xuebing LI: "Synthesis of Chitosan-Sugar Hybrid and Evaluation of its Bioactivity"Polymers for Advanced Technologies. 10. 455-458 (1999)

[文献書誌] Y.Suzuki: "Influence of physico-chemical properties of chitin and chitosan on complement activation"Carbhydr.Polym.. (in printing). (1999)

[文献書誌] Saburo MINAMI: "Drug delivery effects of oxytetracycline-hydrochloride/chitin tablet on dog"Chitin and Chitosan Research. 5. 271-278 (1999)

[文献書誌] Saburo MINAMI: "Veterinary practice with chitin and chitosan"Chitin and Chitinases. 265-277 (1999)

[文献書誌] Minoru MORIMOTO: "Synthesis of water-soluble oxidized chitosan derivatives and their biological activity"Chemistry Letters. 1285-1286 (1999)

[文献書誌] Saburo MINAMI: "Advanced in Chitin Science Vol.2"Jacques Andre Pup.,Lyon,France. 962 (1997)

[文献書誌] Yoshiharu OKAMOTO: "Advanced in Chitin Science Vol.2"Jacques Andre Pup.,Lyon,France. 962 (1997)

[文献書誌] Saburo MINAMI: "Advanced in Chitin Science Vol.3"Rita advertising Co.,Ltd. 516 (1998)

[文献書誌] Yoshiharu OKAMOTO: "Advanced in Chitin Science Vol.3"Rita advertising Co.,Ltd. 516 (1998)

[文献書誌] Saburo MINAMI: "23ed Aystralasian Polymer Symposium"Deakin University. 530 (1999)

[文献書誌] Yoshiharu OKAMOTO: "23ed Aystralasian Polymer Symposium"Deakin University. 530 (1999)

1999 年度実績報告書

南三郎鳥取大学, 農学部, 教授 (70032307)

[文献書誌] 南三郎: "乳汁食細胞に及ぼすキトサン乳槽内投与の効果"日獣会誌. 50. 143-146 (1997)

[文献書誌] 重政好弘: "キチン及びキトサンの医学領域への応用"高分子加工. 46. 75-81 (1997)

[文献書誌] 南三郎: "キチン及びその誘導体の医学・獣医学応用"獣医畜産新報. 50. 1033-1052 (1997)

[文献書誌] 南三郎: "動物繊維キチンおよびその誘導体の生体活性効果と動物臨床応用"Sen'I Gakkaishi. 54. 266-271 (1998)

[文献書誌] 南三郎: "キチン質の創傷癒促進進機構"チキン・キトサン研究. 5. 1-13 (1999)