1999 年度実績報告書

高等植物の自家不和合性における自他識別機構の分子的解析

研究課題

研究課題/領域番号	11238101
研究種目	特定領域研究(B)
研究機関	奈良先端科学技術大学院大学
研究代表者	磯貝彰奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)
研究分担者	鈴木昭憲秋田県立大学, 学長 (90011907) 崎山文夫大阪大学, 名誉教授 (40029947) 神山康夫三重大学, 生物資源学部, 教授 (80024579) 日向康吉東北大学, 名誉教授 (00005589) 村田紀夫岡崎基礎生物学研究所, 教授 (90011569)
キーワード	自家不和合性 / 自他識別 / S遺伝子 / 胞子体型 / 配偶体型
研究概要	本研究は、特定領域研究(B)「高等植物の自家不和合性における自他識別機構の分子的解析」5つの計画研究を統括し、調整し、推進するために策定された。そのための方策として、まず、各研究課題の研究の現状と研究の目的を、本研究に携わる研究者があらためて相互に理解し、情報や技術の交換をはかり、協同研究体制を作っていく必要がある。そこで、第1回の総括班会議では、総括班員と個別研究課題の代表者が合同で、この研究プロジェクトの今後の予定、研究の進め方について議論した。また、総括班会議に続いて、本プロジェクトにかかわる研究者薬60名の参加の下に、研究発表会を行い、活発な討論を行った。また、総括班の評価委員からも研究の進め方、研究内容なとについて講評があった。特に、本研究の最初の課題であるアブラナ科植物の自家不和合性に関与する花粉因子の研究の現状については、近々の内に一定の結論が得られるものと評価され、それは、これまでの実質的な協同研究の大きな成果であるとされた。事実、総括班会議後まもなく、花粉因子の同定のための最終的な実験結果が得られ、本年度の主要な研究成果として論文に印刷された。なお、総括班としては、本年度の成果のとりまとめとして、個別の研究の成果の概要と印刷された論文集をまとめた成果報告書を作成し、総括班員、研究代表者、研究分担者、共同研究者および関係各方面に配布して、その成果について、広く公表することとした。来年度以降の総括班としての計画としては、年2回の総括班会議、研究発表会、及び、若手研究者の研修会を予定し、研究発表会の機会を利用して海外の研究者を招聘し、交流を図ることが決定された。

研究成果
(25件)

すべてその他

すべて文献書誌 (25件)

[文献書誌] T.Entani: "Centromeric localization of an S-RNase gene in Petunia hybrida Vilm"Theopr.Appl.Genet.,. 99. 391-397 (1999)
[文献書誌] M.watanabe: "Molecular biology of self-incompatibility in Brassica species"PlantBiotechnol.,. 16(4). 263-272 (1999)
[文献書誌] G.Suzuki: "Genomic oraganization of the S locus: Identification and charasterization of genes in SLG/SRK region of S9 haplotype of Brassica campestris(syn.rapa)"Genetics. 153. 391-400 (1999)
[文献書誌] T.takasaki: "Introduction of SLG(S locus glycoprotein) alters the phenotype of endogenous S haplotype,but confers no new S haplotype sepcificity in Brassica rapa L."Plant Mol.Biol.,. 40. 659-668 (1999)
[文献書誌] M.watanabe: "Two anther-expressed genes downstream of SLG9: Identification of a novel S-linked gene specifically expressed in anthers at the uninucleate of Brassica campestris(syn.rapa)L"Sex.Plant Reprod.. 12. 127-134 (1999)
[文献書誌] T.Entani: "Relationship between polyploydy and pollen self-incompatibility phenotype in Petunis hybrida Vilm"Biosci.Biotechnol.Biochem.,. 63(11). 1882-1888 (1999)
[文献書誌] T.Takasaki: "The S receptor Kinase determines self-incompatibility in Brassica stigma"Nature. (in press).
[文献書誌] S.Takayama: "The pollen determinant of self-incompatibility in Brassica compestris"Proc.Natl.Acad.Sci.USA. (in press).
[文献書誌] M.Iwano: "X-ray microanalysis of papillar cells and pollen grains in the pollination process in Brassica using variable-pressure scanning electron microscope"J.Electron Microsc.. (in press).
[文献書誌] S.Takayama: "Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S-locus realted glycoprotein 1 involved in pollen-stigma adhesion"Proc.Natl Acad.Sci.USA. (in press).
[文献書誌] Akiko Sasaki: "Partial purification and characterization of a novel endo-b-mannosidase acting on N-linked sugar chains from Lilium longflorum Thumb"J.Biochem.. 125. 363-367 (1999)
[文献書誌] Takeshi Ishimizu: "PCR-based method for determination of S-genotype of Japanese pear"Theor.Appl.Genet.. 98. 961-967 (1999)
[文献書誌] Takeshi Ishimizu: "Presence of Asn-linked N-acetylglucosamine and chitobiose in Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility"Eur.J.Biochem.. 263. 624-634 (1999)
[文献書誌] Kusaba M.: "Sequence and structural diversity of the S locus genes from different lines with the same self-recognition specificities in Brassica oleracea"Genetics. 154. 413-420 (2000)
[文献書誌] Nishio T.: "Sequence diversity of SLG and SRK in Brassica oleracea L"Ann.Bot.. (in press).
[文献書誌] Suzuki G.: "physical distances between S-locus genes in various S haplotypes of Brassica rapa and B. oleracea"Theor.Appl.Genet.. (in press).
[文献書誌] Sakamoto K.: "Single seed PCR-RFLP analysis for identification of S haplotypes in commercial F1 hybrid cultivars of broccoli and cabbage"Plant Cell Rep.. (in press).
[文献書誌] Okazaki K.: "Characterization of S tester lines in Brassica oleracea: polymorphism of restriction fragment length of SLG homologues and isoelectric points of S-locus glycoproteins"Theor.Appl.Genet.. 98. 1329-1334 (1999)
[文献書誌] Jordan,N.D.: "S-protein mutants indicate a functional role for SBP in the self-incompatibility reaction of Papaver rhoeas"Plant J.. 20. 119-125 (1999)
[文献書誌] Tomimoto Y.: "A pistil-specific PR-1 like protein Camellia,its expression,sequence and gene-alogical position"Breed.Sci. 40. 97-104 (1999)
[文献書誌] Watanabe I.: "Diversity of Azolla japonica,analyzed by random amplified polymorphic DNA"J.Japan.Bot.. 74. 142-149 (1999)
[文献書誌] Hashizume F.: "Efficient Agrobacterium-mediated transformation and the usefulness of a synthetic GFP reporter gene in leading varieties of Japanica rice"Plant Biotechnol. 16. 397-401 (1999)
[文献書誌] Hobo T.: "ACGT-containing abscisic acid response element(ABRE) and coupling element 3(CE3) are functionally equivalent"Plant J.. 19. 679-689 (1999)
[文献書誌] Hobo T.: "A bZIP facter,TRAB1,interacts with VP1 and mediates abscisic acid-induced trans-cription"Proc.Natl.Acad.Sci.USA. 96. 15348-15353 (1999)
[文献書誌] Kowyama Y.: "Sporophytic self-incompatibility of Ipomoea rifida,a close relative to sweet potato"Ann.Bot.. (in press).

1999 年度 実績報告書

高等植物の自家不和合性における自他識別機構の分子的解析

研究代表者

磯貝 彰 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)

研究成果

[文献書誌] T.Entani: "Centromeric localization of an S-RNase gene in Petunia hybrida Vilm"Theopr.Appl.Genet.,. 99. 391-397 (1999)

[文献書誌] M.watanabe: "Molecular biology of self-incompatibility in Brassica species"PlantBiotechnol.,. 16(4). 263-272 (1999)

[文献書誌] G.Suzuki: "Genomic oraganization of the S locus: Identification and charasterization of genes in SLG/SRK region of S9 haplotype of Brassica campestris(syn.rapa)"Genetics. 153. 391-400 (1999)

[文献書誌] T.takasaki: "Introduction of SLG(S locus glycoprotein) alters the phenotype of endogenous S haplotype,but confers no new S haplotype sepcificity in Brassica rapa L."Plant Mol.Biol.,. 40. 659-668 (1999)

[文献書誌] M.watanabe: "Two anther-expressed genes downstream of SLG9: Identification of a novel S-linked gene specifically expressed in anthers at the uninucleate of Brassica campestris(syn.rapa)L"Sex.Plant Reprod.. 12. 127-134 (1999)

[文献書誌] T.Entani: "Relationship between polyploydy and pollen self-incompatibility phenotype in Petunis hybrida Vilm"Biosci.Biotechnol.Biochem.,. 63(11). 1882-1888 (1999)

[文献書誌] T.Takasaki: "The S receptor Kinase determines self-incompatibility in Brassica stigma"Nature. (in press).

[文献書誌] S.Takayama: "The pollen determinant of self-incompatibility in Brassica compestris"Proc.Natl.Acad.Sci.USA. (in press).

[文献書誌] M.Iwano: "X-ray microanalysis of papillar cells and pollen grains in the pollination process in Brassica using variable-pressure scanning electron microscope"J.Electron Microsc.. (in press).

[文献書誌] S.Takayama: "Isolation and characterization of pollen coat proteins of Brassica campestris that interact with S-locus realted glycoprotein 1 involved in pollen-stigma adhesion"Proc.Natl Acad.Sci.USA. (in press).

[文献書誌] Akiko Sasaki: "Partial purification and characterization of a novel endo-b-mannosidase acting on N-linked sugar chains from Lilium longflorum Thumb"J.Biochem.. 125. 363-367 (1999)

[文献書誌] Takeshi Ishimizu: "PCR-based method for determination of S-genotype of Japanese pear"Theor.Appl.Genet.. 98. 961-967 (1999)

[文献書誌] Takeshi Ishimizu: "Presence of Asn-linked N-acetylglucosamine and chitobiose in Pyrus pyrifolia S-RNases associated with gametophytic self-incompatibility"Eur.J.Biochem.. 263. 624-634 (1999)

[文献書誌] Kusaba M.: "Sequence and structural diversity of the S locus genes from different lines with the same self-recognition specificities in Brassica oleracea"Genetics. 154. 413-420 (2000)

[文献書誌] Nishio T.: "Sequence diversity of SLG and SRK in Brassica oleracea L"Ann.Bot.. (in press).

[文献書誌] Suzuki G.: "physical distances between S-locus genes in various S haplotypes of Brassica rapa and B. oleracea"Theor.Appl.Genet.. (in press).

[文献書誌] Sakamoto K.: "Single seed PCR-RFLP analysis for identification of S haplotypes in commercial F1 hybrid cultivars of broccoli and cabbage"Plant Cell Rep.. (in press).

[文献書誌] Okazaki K.: "Characterization of S tester lines in Brassica oleracea: polymorphism of restriction fragment length of SLG homologues and isoelectric points of S-locus glycoproteins"Theor.Appl.Genet.. 98. 1329-1334 (1999)

[文献書誌] Jordan,N.D.: "S-protein mutants indicate a functional role for SBP in the self-incompatibility reaction of Papaver rhoeas"Plant J.. 20. 119-125 (1999)

[文献書誌] Tomimoto Y.: "A pistil-specific PR-1 like protein Camellia,its expression,sequence and gene-alogical position"Breed.Sci. 40. 97-104 (1999)

[文献書誌] Watanabe I.: "Diversity of Azolla japonica,analyzed by random amplified polymorphic DNA"J.Japan.Bot.. 74. 142-149 (1999)

[文献書誌] Hashizume F.: "Efficient Agrobacterium-mediated transformation and the usefulness of a synthetic GFP reporter gene in leading varieties of Japanica rice"Plant Biotechnol. 16. 397-401 (1999)

[文献書誌] Hobo T.: "ACGT-containing abscisic acid response element(ABRE) and coupling element 3(CE3) are functionally equivalent"Plant J.. 19. 679-689 (1999)

[文献書誌] Hobo T.: "A bZIP facter,TRAB1,interacts with VP1 and mediates abscisic acid-induced trans-cription"Proc.Natl.Acad.Sci.USA. 96. 15348-15353 (1999)

[文献書誌] Kowyama Y.: "Sporophytic self-incompatibility of Ipomoea rifida,a close relative to sweet potato"Ann.Bot.. (in press).

1999 年度実績報告書

磯貝彰奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (20011992)