1999 年度実績報告書

HGFによる形態形成および器官形成・再生の分子生物的解析

研究課題

研究課題/領域番号	11308025
研究種目	基盤研究(A)
研究機関	大阪大学
研究代表者	中村敏一大阪大学, 医学系研究科, 教授 (00049397)
研究分担者	船越洋大阪大学, 医学系研究科, 助手 (40273685) 小清水右一大阪大学, 医学系研究科, 助手 (50281126) 松本邦夫大阪大学, 医学系研究科, 助教授 (90201780)
キーワード	HGF / c-met / トランスジェニックマウス / 神経 / HGFアンタゴニスト / 筋萎縮性側索硬化症 / ノックアウトマウス / ES細胞
研究概要	1.神経特異的HGF発現動物の作製とその解析成体神経組織に特異的な発現を呈するneuron-specific enolaseプロモーターの下流にHGF cDNAを連結し、トランスジェニック(Tg)マウスの作製を行った。遺伝子導入が確認できた9系統中、6系統で神経特異的なHGF発現・産生が認められた。胎児および成体の脳・神経組織には特に異常はみられなかった。現在、HGFの神経栄養因子としての機能を検討するため、これらのマウスの筋萎縮性側索硬化症(ALS)モデルのSOD-1 Tgマウスと交配し、神経の組織形態および機能に及ぼす影響を経時的に解析している。 2.ケラチノサイト特異的HGF発現動物の作製とその解析ケラチン14プロモーターの下流でHGFを高発現するTgマウスを作製した。このTgマウスでは皮膚や毛には特に異常を認められなかったが、メラノサイトとの異所性増殖がみられた。胎児および成体皮膚組織の詳細な解析により、HGFはメラノサイトの遊走および増殖を促進することが明らかになった。 3.NK4発現動物の作製 HGFの分子内断面はNK4はHGFアンタゴニストとしての活性を有している。メタロチオネインプロモーターの下流でNK4を発現するTgマウスの作製を行った。遺伝子導入が確認できた15系統中、10系統でZn^<2+>投与によるNK4の高い発現誘導がみられた。その主な産生臓器は肝臓、腎臓、小腸であり、系統によっては筋や脳、肺でも高発現がみられた。現在、各種疾患モデル実験系を用いてNK4の器官・組織構築および修復に及ぼす影響を検討している。 4.C-metノックアウトマウスの作製 Cre-LoxPシステムを用いたc-metノックアウトマウスを作製するため、c-metゲノム遺伝子のクローニングを行い、キナーゼ領域の近傍(エクソン14〜18)を含むゲノム断片を基にターゲティングベクターを構築した。現在、このベクターをES細胞に導入して相同組み換え体の作製を進めている。

研究成果
(53件)

すべてその他

すべて文献書誌 (53件)

[文献書誌] T.Ueki,et al.: "Hepatocyte growth factor gene therapy of liver cirrhosis in rats"Nature Medicine. 5. 226-230 (1999)
[文献書誌] Y.Liu,et al.: "Upregulation of hepatocyte growth factor receptor : an amplification and targeting mechanism for hepatocyte growth factor action in acute renal failure"Kidney Intern. 55. 442-453 (1999)
[文献書誌] M.Tahara, K.Matsumoto, T.Nukiwa, and T.Nakamura: "Hepatocyte gowth factor leads to recovery from alcohol-induced fatty liver in rats"J.Clin.Invest.. 103. 313-320 (1999)
[文献書誌] J.Wojta, et al.: "Hepatocyte growth factor increases expression of vascular endothelial growth factor and plasminogen activator inhibitor-1 in human keratinocytes and the vascular endothelial growth factor receptor flk-1 in human endothelial cells. Evidence for a paracrine loop in wound healing"Lab.Invest. 79. 427-438 (1999)
[文献書誌] Z.Jiao, et al.: "Effect of D-galactosamine hydrochloride and partial hepatectomy on spontaneous hepatic injury and hepatocarcinogenesis in Long Evans Cinamon rats"Jpn.J. Cancer Res.. 90. 496-504 (1999)
[文献書誌] H.Azuma,et al.: "Effect of hepatocyte growth factor on chronic rejection in rat renal allografts"Transplat.Proc.. 31. 854-855 (1999)
[文献書誌] S.Takada,et al.: "Effect of hepatocyte growth factor on tacrolimus-induced nephrotoxicity in spontaneously hypertensive rats"Transpl.Int.. 12. 27-32 (1999)
[文献書誌] H.Ueda,et al.: "Gene transfection of hepatocyte growth factor attenuates reperfusion injury in the heart"Annals.Thoracic Surg.. 67. 1726-1731 (1999)
[文献書誌] K.Kosai,K,Matsumoto, and T.Nakamura: "Hepatocyte growth factor prevents endotoxin-induced lethal hepatic failure in mice"Hepatology. 30. 151-159 (1999)
[文献書誌] R.Hasina,et al.: "Autocrine and paracrine motility factors and their involvement in invasiveness in a human oral carcinoma cell line"Brit.J.Cancer. 80. 1708-1717 (1999)
[文献書誌] K.Kitajima,U.Koshimizu and T.Nakamura: "Expression of a novel type of classic cadherin, PB-cadherin in developing brain and limb buds"Develop.Dyn.. 215. 206 (1999)
[文献書誌] N.Taniura-Matsumoto,K.Matsumoto and T.Nakamura: "Prostaglandin production in mouse mammary carcinoma cells confers invasive growth potential by inducing hepatocyte growth factor in fibroblasts"Brit.J.Cancer. 81. 194-202 (1999)
[文献書誌] M.Kato,Y.Kato,T.Nakamura and Y.Sugiyama: "Efficient extraction by the liver governs the overall elimination of hepatocyte growth factor in rats"J.Pharmacol.Exp.Therap.. 290. 373-379 (1999)
[文献書誌] Morishita,et al.: "Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as a cytokine supplement therapy"Hypertention. 33. 1379-1384 (1999)
[文献書誌] W.G.Jiang,et al.: "Hepatocyte growth factor/scatter factor decreases the ixpression of occuludin and transendothelial resistance(TER) and increased paracellular permeability in human vascular endothelial cells"J.Cell.Physiol.. 181. 319-329 (1999)
[文献書誌] C.C.Fehlner-Gardiner,et al.: "Characterization of a functional relationship between hepatocyte growth factor and mouse bone marrow-derived mast cells"Differentiation. 65. 27-42 (1999)
[文献書誌] S.Deleu,et al.: "Immediate early gene expression in dog thyrocytes in response to growth proliferation and differentiation stimuli"J.Cell.Physiol.. 181. 342-354 (1999)
[文献書誌] S.Deleu,et al.: "IGF-1 or insulin,and the TSH cyclic AMP cascade separately control dog and human thyroid cell growth and DNA synthesis, and complement each other in inducing mitogenesis"Mole.Cell.Endocrin.. 149. 41-51 (1999)
[文献書誌] S.Hayashi,et al.: "Potential role of hepatocyte growth factor, a novel angiogenic growth factor in peripheral arterial disease : Downregulation of HGF in response to hypoxia in vascular cells"Circulation. 100. II301-II308 (1999)
[文献書誌] T.Nagahori,et al.: "Interferon-gamma upregulates the c-Met/HGF receptor expression in alvedar epithelial cells"Am.J.Respir.Cell Mol.Biol.. 21. 490-497 (1999)
[文献書誌] O.Amano,U.Koshimizu,T.Nakamura and S.Iseki: "Enhancement by hepatocyte growth factor (HGF) of bone and cartilage formation during embryonic mouse mandibular development in vitro"Arch.Oral Biol.. 44. 935-946 (1999)
[文献書誌] K.Nishimura,et al.: "Prostate stromal cell-derived hepatocyte growth factor induces invasion of prostate cancer cellls DU 145 through Tumor-stromal interaction"Prostate. 41. 145-153 (1999)
[文献書誌] W.Sun,H.Funakoshi and T.Nakamura: "Differential expression of hepatocyte growth factor and its receptor, c-Met in the rat retina during development"Brain Res.. 851. 46-53 (1999)
[文献書誌] A.Uchiyama,et al.: "Hepatocyte growth factor and invasion-stimulatory activity are induced in pleural fluid by surgery in lung cancer patients"Br.J.Cancer. 81. 721-726 (1999)
[文献書誌] K.Matsumoto,et al.: "Prevention of renal damage by Angiotensin II blockade, accompanied by increased renal hepatocyte growth factor in experimental hypertensive rats"Hypertension. 34. 279-284 (1999)
[文献書誌] Y.Okura,et al.: "Analysis of neurotrophic factor of hepatocyte growth factor (HGF) in the adult hypoglossal nerve axotomy model"Eur J.Neurosci.. 11. 4139-4144 (1999)
[文献書誌] T.Sumi,K.Matsumoto,Y.Takai and T.Nakamura: "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by Rho-and Cdc42-activated LIM-kinase2"J.Cell Biol.. 147. 1519-1532 (1999)
[文献書誌] MK.Jones,et al.: "HGF triggers activation of Cox-2 gene in rat gastric epithelial cells Action mediated through the ERK2 signalling pathway"FASEB J.. 13. 2186-2194 (1999)
[文献書誌] Y.Kawano,et al.: "Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo"J.Cell Biol.. 147. 1023-1037 (1999)
[文献書誌] F.Tamura,et al.: "FK506 promotes liver regeneration by suppressing natural killer cell activity"J.Gastroenterol.and Hepatol.. (in press). (2000)
[文献書誌] A.Uchiyama,et al.: "Significance of locally-secreted hepatocyte growth factor after thoracic cancer surgery as a stimulator for postoperative cancer invasion."Jpn.J.Clin.Oncol.. (in press). (2000)
[文献書誌] W.G.Jiang,et al.: "Antagonistic effect of NK4,a novel HGF variant, on in vitro angiogenesis of human vascular endothelial cells"Clin.Cancer Res.. (in press). (2000)
[文献書誌] S.Hiscox,et al.: "Inhibition of HGF/SF-induced breast cancer cell matility and invasion by the HGF/SF variant, NK4."Breast Cancer Res.& Treat.. (in press). (2000)
[文献書誌] M.Nakano,et al.: "Hepatocyte growth factor is essential for amelioration of hyperglycemia in strepto zotocin-induced diabetic mice receiving a marginal mass of intrahepatic islet grafts"Transplantation. (in press). (2000)
[文献書誌] T.Yamada,et al.: "Enhanced expression of hepatocyte growth factor by pulmonary ischemia reperfusion injury in the rat"Am.J.Resp.Crit Care Med.. (in press). (2000)
[文献書誌] S.Mizuno,et al.: "Reciprocal balance between HGF and TGF-β a key determinant for pathological phenotypes in chronic renal disease"Kidney Intern.. (in press). (2000)
[文献書誌] C.Parr,et al.: "NK4, a new HGF/SF variant,is an antagonist to the influence of HGF/SF on the matility and invasion of colon cancer cells"Intern.J.Cancer. (in press). (2000)
[文献書誌] 中村敏一: "新しい腫瘍血管新生抑制物質-malignostatin(NK4)."細胞工学. 18. 223-225 (1999)
[文献書誌] 久場敬司、松本邦夫、中村敏一: "HGFによる血管新生とその分子内クリングル断片(NK4)による腫瘍血管新生阻害"実験医学. 17. 734-740 (1999)
[文献書誌] 水野信哉、中村敏一: "HGFによる急性および慢性腎不全の治療"腎と透析. 46. 494-498 (1999)
[文献書誌] 久場敬司、中村敏一: "HGFと癌の浸潤・転移"Surgery Frontier 6(No2):27-38. 6. 27-38 (1999)
[文献書誌] 小清水右一、中村敏一: "HGF-肝細胞癌に対するHGFの抗腫瘍作用"血液・免疫・腫瘍. 4. 40-45 (1999)
[文献書誌] 中村隆弘、中村敏一: "肝再生の分子機序"別冊:医学のあゆみ、消化器疾患. 2. 68-73 (1999)
[文献書誌] 久場敬司、中村敏一: "医学研究その15:血管新生(Angiogenesis)"The Lung. 7. 94-99 (1999)
[文献書誌] 水野信哉、中村敏一: "肝細胞増殖因子による腎再生"最新医学. 54. 59-69 (1999)
[文献書誌] 角智行、中村敏一: "細胞遊走における増殖因子の役割"医学のあゆみ. 191. 137-142 (1999)
[文献書誌] 水野信哉、中村敏一: "肝再生とHGF"現代医療. 31. 69-78 (1999)
[文献書誌] 松本邦夫、中村敏一: "HGFの臨床応用に向けて"Medical Academy News. 720/721. 12-12 (1999)
[文献書誌] 船越洋、中村敏一: "肝細胞増殖因子(HGF)の検査値と各種疾患"日本臨牀. 37. 821-826 (1999)
[文献書誌] 小清水右一中村敏一: "形質転換成長因子(HGF)"日本臨牀. 37. 802-804 (1999)
[文献書誌] 小清水右一、大熊勝治: "肝細胞の培養法"細胞生物学実験法. 1. 27-47 (1999)
[文献書誌] 中村敏一、小池克郎、関谷剛男、近藤寿人: "タンパク質の分析法"分子生物学プロトコール改訂2版. 11章. 269-284 (1999)
[文献書誌] 中村敏一、長澤俊彦: "HGFによる腎疾患の治療"Annual Review 腎臓 2000. XII. 233-240 (2000)

1999 年度 実績報告書

HGFによる形態形成および器官形成・再生の分子生物的解析

研究代表者

中村 敏一 大阪大学, 医学系研究科, 教授 (00049397)

研究成果

[文献書誌] T.Ueki,et al.: "Hepatocyte growth factor gene therapy of liver cirrhosis in rats"Nature Medicine. 5. 226-230 (1999)

[文献書誌] Y.Liu,et al.: "Upregulation of hepatocyte growth factor receptor : an amplification and targeting mechanism for hepatocyte growth factor action in acute renal failure"Kidney Intern. 55. 442-453 (1999)

[文献書誌] M.Tahara, K.Matsumoto, T.Nukiwa, and T.Nakamura: "Hepatocyte gowth factor leads to recovery from alcohol-induced fatty liver in rats"J.Clin.Invest.. 103. 313-320 (1999)

[文献書誌] Z.Jiao, et al.: "Effect of D-galactosamine hydrochloride and partial hepatectomy on spontaneous hepatic injury and hepatocarcinogenesis in Long Evans Cinamon rats"Jpn.J. Cancer Res.. 90. 496-504 (1999)

[文献書誌] H.Azuma,et al.: "Effect of hepatocyte growth factor on chronic rejection in rat renal allografts"Transplat.Proc.. 31. 854-855 (1999)

[文献書誌] S.Takada,et al.: "Effect of hepatocyte growth factor on tacrolimus-induced nephrotoxicity in spontaneously hypertensive rats"Transpl.Int.. 12. 27-32 (1999)

[文献書誌] H.Ueda,et al.: "Gene transfection of hepatocyte growth factor attenuates reperfusion injury in the heart"Annals.Thoracic Surg.. 67. 1726-1731 (1999)

[文献書誌] K.Kosai,K,Matsumoto, and T.Nakamura: "Hepatocyte growth factor prevents endotoxin-induced lethal hepatic failure in mice"Hepatology. 30. 151-159 (1999)

[文献書誌] R.Hasina,et al.: "Autocrine and paracrine motility factors and their involvement in invasiveness in a human oral carcinoma cell line"Brit.J.Cancer. 80. 1708-1717 (1999)

[文献書誌] K.Kitajima,U.Koshimizu and T.Nakamura: "Expression of a novel type of classic cadherin, PB-cadherin in developing brain and limb buds"Develop.Dyn.. 215. 206 (1999)

[文献書誌] N.Taniura-Matsumoto,K.Matsumoto and T.Nakamura: "Prostaglandin production in mouse mammary carcinoma cells confers invasive growth potential by inducing hepatocyte growth factor in fibroblasts"Brit.J.Cancer. 81. 194-202 (1999)

[文献書誌] M.Kato,Y.Kato,T.Nakamura and Y.Sugiyama: "Efficient extraction by the liver governs the overall elimination of hepatocyte growth factor in rats"J.Pharmacol.Exp.Therap.. 290. 373-379 (1999)

[文献書誌] Morishita,et al.: "Therapeutic angiogenesis induced by human recombinant hepatocyte growth factor in rabbit hind limb ischemia model as a cytokine supplement therapy"Hypertention. 33. 1379-1384 (1999)

[文献書誌] W.G.Jiang,et al.: "Hepatocyte growth factor/scatter factor decreases the ixpression of occuludin and transendothelial resistance(TER) and increased paracellular permeability in human vascular endothelial cells"J.Cell.Physiol.. 181. 319-329 (1999)

[文献書誌] C.C.Fehlner-Gardiner,et al.: "Characterization of a functional relationship between hepatocyte growth factor and mouse bone marrow-derived mast cells"Differentiation. 65. 27-42 (1999)

[文献書誌] S.Deleu,et al.: "Immediate early gene expression in dog thyrocytes in response to growth proliferation and differentiation stimuli"J.Cell.Physiol.. 181. 342-354 (1999)

[文献書誌] S.Deleu,et al.: "IGF-1 or insulin,and the TSH cyclic AMP cascade separately control dog and human thyroid cell growth and DNA synthesis, and complement each other in inducing mitogenesis"Mole.Cell.Endocrin.. 149. 41-51 (1999)

[文献書誌] S.Hayashi,et al.: "Potential role of hepatocyte growth factor, a novel angiogenic growth factor in peripheral arterial disease : Downregulation of HGF in response to hypoxia in vascular cells"Circulation. 100. II301-II308 (1999)

[文献書誌] T.Nagahori,et al.: "Interferon-gamma upregulates the c-Met/HGF receptor expression in alvedar epithelial cells"Am.J.Respir.Cell Mol.Biol.. 21. 490-497 (1999)

[文献書誌] O.Amano,U.Koshimizu,T.Nakamura and S.Iseki: "Enhancement by hepatocyte growth factor (HGF) of bone and cartilage formation during embryonic mouse mandibular development in vitro"Arch.Oral Biol.. 44. 935-946 (1999)

[文献書誌] K.Nishimura,et al.: "Prostate stromal cell-derived hepatocyte growth factor induces invasion of prostate cancer cellls DU 145 through Tumor-stromal interaction"Prostate. 41. 145-153 (1999)

[文献書誌] W.Sun,H.Funakoshi and T.Nakamura: "Differential expression of hepatocyte growth factor and its receptor, c-Met in the rat retina during development"Brain Res.. 851. 46-53 (1999)

[文献書誌] A.Uchiyama,et al.: "Hepatocyte growth factor and invasion-stimulatory activity are induced in pleural fluid by surgery in lung cancer patients"Br.J.Cancer. 81. 721-726 (1999)

[文献書誌] K.Matsumoto,et al.: "Prevention of renal damage by Angiotensin II blockade, accompanied by increased renal hepatocyte growth factor in experimental hypertensive rats"Hypertension. 34. 279-284 (1999)

[文献書誌] Y.Okura,et al.: "Analysis of neurotrophic factor of hepatocyte growth factor (HGF) in the adult hypoglossal nerve axotomy model"Eur J.Neurosci.. 11. 4139-4144 (1999)

[文献書誌] T.Sumi,K.Matsumoto,Y.Takai and T.Nakamura: "Cofilin phosphorylation and actin cytoskeletal dynamics regulated by Rho-and Cdc42-activated LIM-kinase2"J.Cell Biol.. 147. 1519-1532 (1999)

[文献書誌] MK.Jones,et al.: "HGF triggers activation of Cox-2 gene in rat gastric epithelial cells Action mediated through the ERK2 signalling pathway"FASEB J.. 13. 2186-2194 (1999)

[文献書誌] Y.Kawano,et al.: "Phosphorylation of myosin-binding subunit (MBS) of myosin phosphatase by Rho-kinase in vivo"J.Cell Biol.. 147. 1023-1037 (1999)

[文献書誌] F.Tamura,et al.: "FK506 promotes liver regeneration by suppressing natural killer cell activity"J.Gastroenterol.and Hepatol.. (in press). (2000)

[文献書誌] A.Uchiyama,et al.: "Significance of locally-secreted hepatocyte growth factor after thoracic cancer surgery as a stimulator for postoperative cancer invasion."Jpn.J.Clin.Oncol.. (in press). (2000)

[文献書誌] W.G.Jiang,et al.: "Antagonistic effect of NK4,a novel HGF variant, on in vitro angiogenesis of human vascular endothelial cells"Clin.Cancer Res.. (in press). (2000)

[文献書誌] S.Hiscox,et al.: "Inhibition of HGF/SF-induced breast cancer cell matility and invasion by the HGF/SF variant, NK4."Breast Cancer Res.& Treat.. (in press). (2000)

[文献書誌] M.Nakano,et al.: "Hepatocyte growth factor is essential for amelioration of hyperglycemia in strepto zotocin-induced diabetic mice receiving a marginal mass of intrahepatic islet grafts"Transplantation. (in press). (2000)

[文献書誌] T.Yamada,et al.: "Enhanced expression of hepatocyte growth factor by pulmonary ischemia reperfusion injury in the rat"Am.J.Resp.Crit Care Med.. (in press). (2000)

[文献書誌] S.Mizuno,et al.: "Reciprocal balance between HGF and TGF-β a key determinant for pathological phenotypes in chronic renal disease"Kidney Intern.. (in press). (2000)

[文献書誌] C.Parr,et al.: "NK4, a new HGF/SF variant,is an antagonist to the influence of HGF/SF on the matility and invasion of colon cancer cells"Intern.J.Cancer. (in press). (2000)

[文献書誌] 中村敏一: "新しい腫瘍血管新生抑制物質-malignostatin(NK4)."細胞工学. 18. 223-225 (1999)

[文献書誌] 久場敬司、松本邦夫、中村敏一: "HGFによる血管新生とその分子内クリングル断片(NK4)による腫瘍血管新生阻害"実験医学. 17. 734-740 (1999)

[文献書誌] 水野信哉、中村敏一: "HGFによる急性および慢性腎不全の治療"腎と透析. 46. 494-498 (1999)

[文献書誌] 久場敬司、中村敏一: "HGFと癌の浸潤・転移"Surgery Frontier 6(No2):27-38. 6. 27-38 (1999)

[文献書誌] 小清水右一、中村敏一: "HGF-肝細胞癌に対するHGFの抗腫瘍作用"血液・免疫・腫瘍. 4. 40-45 (1999)

[文献書誌] 中村隆弘、中村敏一: "肝再生の分子機序"別冊:医学のあゆみ、消化器疾患. 2. 68-73 (1999)

[文献書誌] 久場敬司、中村敏一: "医学研究その15:血管新生(Angiogenesis)"The Lung. 7. 94-99 (1999)

[文献書誌] 水野信哉、中村敏一: "肝細胞増殖因子による腎再生"最新医学. 54. 59-69 (1999)

[文献書誌] 角 智行、中村敏一: "細胞遊走における増殖因子の役割"医学のあゆみ. 191. 137-142 (1999)

[文献書誌] 水野信哉、中村敏一: "肝再生とHGF"現代医療. 31. 69-78 (1999)

[文献書誌] 松本邦夫、中村敏一: "HGFの臨床応用に向けて"Medical Academy News. 720/721. 12-12 (1999)

[文献書誌] 船越 洋、中村敏一: "肝細胞増殖因子(HGF)の検査値と各種疾患"日本臨牀. 37. 821-826 (1999)

[文献書誌] 小清水右一中村敏一: "形質転換成長因子(HGF)"日本 臨牀. 37. 802-804 (1999)

[文献書誌] 小清水右一、大熊勝治: "肝細胞の培養法"細胞生物学実験法. 1. 27-47 (1999)

[文献書誌] 中村敏一、小池克郎、関谷剛男、近藤寿人: "タンパク質の分析法"分子生物学プロトコール 改訂2版. 11章. 269-284 (1999)

[文献書誌] 中村敏一、長澤俊彦: "HGFによる腎疾患の治療"Annual Review 腎臓 2000. XII. 233-240 (2000)

1999 年度実績報告書

中村敏一大阪大学, 医学系研究科, 教授 (00049397)

[文献書誌] 角智行、中村敏一: "細胞遊走における増殖因子の役割"医学のあゆみ. 191. 137-142 (1999)

[文献書誌] 船越洋、中村敏一: "肝細胞増殖因子(HGF)の検査値と各種疾患"日本臨牀. 37. 821-826 (1999)

[文献書誌] 小清水右一中村敏一: "形質転換成長因子(HGF)"日本臨牀. 37. 802-804 (1999)

[文献書誌] 中村敏一、小池克郎、関谷剛男、近藤寿人: "タンパク質の分析法"分子生物学プロトコール改訂2版. 11章. 269-284 (1999)