2000 年度実績報告書

神経可塑性の発生・分化におけるIP3受容体Ca^<2+>シグナリングの役割

研究課題

研究課題/領域番号	11308032
研究機関	東京大学
研究代表者	御子柴克彦東京大学, 医科学研究所, 教授 (30051840)
キーワード	レーザー光 / マラカイトグリーン / Ca2+ / IP3
研究概要	レーザー光を利用した機能分子不活性化法[CALI (chromophore assisted laser inactivation)]による神経成長円錐の形成及び脳の発生・分化のメカニズムの解析を進めた。特異抗体(これにマラカイトグリーンという色素をラベルする)を細胞内へ導入して、レーザー光照射により、ラジカルを放出させて、特異的に標的である抗原分子の破壊を試みた。これは細胞内の一部に照射する方法のみならず、ビッグレーザーを利用して、レーザー光を利用して、組織レベル及び個体レベルへ応用した。 IP3吸収分子(IP3受容体のIP3結合ドメインに変異を加えることにより結合親和性が1000倍高い)の過剰発現を利用してIP3の発生・分化及び生理機能への役割をニューロン内へ導入した際の細胞内Ca2+動態と、機能・形態への影響、発生過程でのシュペーマン形成体の役割を明らかにし、具体的には各細胞群へ導入することによりCa2+と神経誘導、発生・分化との関連を調べた。

研究成果
(20件)

すべてその他

すべて文献書誌 (20件)

[文献書誌] Takei,K.,: "Regulation of nearve drowth mediated by inositol 1,4,5-trisphosphate receptors in growth cones."Science. 282. 1705-1708 (1998)
[文献書誌] Zhao,H.,: "Functional expression of a mammalian odorant receptor."Science. 279. 237-242 (1998)
[文献書誌] Kume,S.,: "Role of inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos."Science. 278. 1940-1943 (1997)
[文献書誌] Del Rio,J.A.,: "A role for Cajal-Retzius cells and reelin in the development of hippocampal connections."Nature. 385. 70-74 (1997)
[文献書誌] Sheldon,M.,: "scrambler and yotari : spontaneous mutations that disrupt the mouse disabled-1 gene and lead to a reeler-like phenotype."Nature. 389. 730-733 (1997)
[文献書誌] Umemori,H.,: "Activation of the G Protein Gq/11 Through Tyrosine Phosphorylation of the α Subunit."J.Neurosci. 17. 23-31 (1997)
[文献書誌] Matsumoto,M.,: "Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor."Nature. 379. 168-171 (1996)
[文献書誌] Fukuda,M.,: "Phospholipid composition dependenceCa^<2+>-dependent phospholipid binding to the C2A domain of synaptotagmin IV."J.Biol.Chem. 271. 8430-8434 (1996)
[文献書誌] Fukuda,M.: "Structure-function relationships of the mouse Gap1^m (Determination of the inositol 1,3,4,5-tetrakisphosphate-binding domain)."J.Biol.Chem. 271. 18838-18842 (1996)
[文献書誌] Yamaguchi,Y.,: "Myelin proteolipid protein (PLP), but not DM-20, is an inositol hexakisphosphate binding protein."J.Biol.Chem. 271. 27838-27846 (1996)
[文献書誌] Monkawa,T.,: "Heterotetrameric complex formation of inositol 1,4,5-trisphosphate receptor subunits."J.Biol.Chem. 270. 14700-14704 (1995)
[文献書誌] Honda,Z.,: "Gq Pathway Desensitizes Chemotactic Receptor-induced Calcium Signaling via Inositol Trisphosphate Receptor Down-regulation"J.Biol.Chem. 270. 4840-4844 (1995)
[文献書誌] Fukuda,M.,: "Functional versity of C2 domains of synaptotagmin family."J.Biol.Chem. 270. 26523-26527 (1995)
[文献書誌] Fukuda,M.,: "Role of the C2B domain of synaptotagmin in vesicular release and recycling as determined by specific antibody injection into the squid giant synapse preterminal."Proc.Natl.Acad.Sci.USA,. 92. 10708-10712 (1995)
[文献書誌] Mikoshiba,K.,: "Role of the C2A domain of synaptotagmin in transmitter release as determined by specific antibody injection into the squid giant synapse preterminal."Proc.Natl.Acad.Sci.USA,92. 92. 10703-10707 (1995)
[文献書誌] Yamamoto-Hino,M.,: "Cloning and characterization of human type 2 and type 3 inositol 1,4,5-trisphosphate receptors."Receptors and Channels.,. 2. 9-22 (1994)
[文献書誌] Nakade,S.,: "Cyclic AMP-depender phosphorylation of an immunoaffinity-purified homotetrameric inositol 1,4,5-trisphosphate receptor (type I) increases Ca^<2+> flux in reconstituted lipid vesicles."J.Biol.Chem.,. 269. 6735-6742 (1994)
[文献書誌] Furuichi,T.,: "Multiple types of ryanodine receptor/Ca^<2+> release channels are differentially expressed in rabbit brain."J.Neurosci.,. 14. 4794-4805 (1994)
[文献書誌] Sasaki,T.,: "Apically localized IP_3 receptors control chloride current in airway gland acinar cells."Am.J.Physiol.,. 267. L152-L158 (1994)
[文献書誌] Kume,S.,: "The xenopus IP_3 receptor : structure, function, and localization in oocvtes and eggs."Cell,. 73. 555-570 (1993)

2000 年度 実績報告書

神経可塑性の発生・分化におけるIP3受容体Ca^<2+>シグナリングの役割

研究代表者

御子柴 克彦 東京大学, 医科学研究所, 教授 (30051840)

研究成果

[文献書誌] Takei,K.,: "Regulation of nearve drowth mediated by inositol 1,4,5-trisphosphate receptors in growth cones."Science. 282. 1705-1708 (1998)

[文献書誌] Zhao,H.,: "Functional expression of a mammalian odorant receptor."Science. 279. 237-242 (1998)

[文献書誌] Kume,S.,: "Role of inositol 1,4,5-trisphosphate receptor in ventral signaling in Xenopus embryos."Science. 278. 1940-1943 (1997)

[文献書誌] Del Rio,J.A.,: "A role for Cajal-Retzius cells and reelin in the development of hippocampal connections."Nature. 385. 70-74 (1997)

[文献書誌] Sheldon,M.,: "scrambler and yotari : spontaneous mutations that disrupt the mouse disabled-1 gene and lead to a reeler-like phenotype."Nature. 389. 730-733 (1997)

[文献書誌] Umemori,H.,: "Activation of the G Protein Gq/11 Through Tyrosine Phosphorylation of the α Subunit."J.Neurosci. 17. 23-31 (1997)

[文献書誌] Matsumoto,M.,: "Ataxia and epileptic seizures in mice lacking type 1 inositol 1,4,5-trisphosphate receptor."Nature. 379. 168-171 (1996)

[文献書誌] Fukuda,M.,: "Phospholipid composition dependenceCa^<2+>-dependent phospholipid binding to the C2A domain of synaptotagmin IV."J.Biol.Chem. 271. 8430-8434 (1996)

[文献書誌] Fukuda,M.: "Structure-function relationships of the mouse Gap1^m (Determination of the inositol 1,3,4,5-tetrakisphosphate-binding domain)."J.Biol.Chem. 271. 18838-18842 (1996)

[文献書誌] Yamaguchi,Y.,: "Myelin proteolipid protein (PLP), but not DM-20, is an inositol hexakisphosphate binding protein."J.Biol.Chem. 271. 27838-27846 (1996)

[文献書誌] Monkawa,T.,: "Heterotetrameric complex formation of inositol 1,4,5-trisphosphate receptor subunits."J.Biol.Chem. 270. 14700-14704 (1995)

[文献書誌] Honda,Z.,: "Gq Pathway Desensitizes Chemotactic Receptor-induced Calcium Signaling via Inositol Trisphosphate Receptor Down-regulation"J.Biol.Chem. 270. 4840-4844 (1995)

[文献書誌] Fukuda,M.,: "Functional versity of C2 domains of synaptotagmin family."J.Biol.Chem. 270. 26523-26527 (1995)

[文献書誌] Fukuda,M.,: "Role of the C2B domain of synaptotagmin in vesicular release and recycling as determined by specific antibody injection into the squid giant synapse preterminal."Proc.Natl.Acad.Sci.USA,. 92. 10708-10712 (1995)

[文献書誌] Mikoshiba,K.,: "Role of the C2A domain of synaptotagmin in transmitter release as determined by specific antibody injection into the squid giant synapse preterminal."Proc.Natl.Acad.Sci.USA,92. 92. 10703-10707 (1995)

[文献書誌] Yamamoto-Hino,M.,: "Cloning and characterization of human type 2 and type 3 inositol 1,4,5-trisphosphate receptors."Receptors and Channels.,. 2. 9-22 (1994)

[文献書誌] Nakade,S.,: "Cyclic AMP-depender phosphorylation of an immunoaffinity-purified homotetrameric inositol 1,4,5-trisphosphate receptor (type I) increases Ca^<2+> flux in reconstituted lipid vesicles."J.Biol.Chem.,. 269. 6735-6742 (1994)

[文献書誌] Furuichi,T.,: "Multiple types of ryanodine receptor/Ca^<2+> release channels are differentially expressed in rabbit brain."J.Neurosci.,. 14. 4794-4805 (1994)

[文献書誌] Sasaki,T.,: "Apically localized IP_3 receptors control chloride current in airway gland acinar cells."Am.J.Physiol.,. 267. L152-L158 (1994)

[文献書誌] Kume,S.,: "The xenopus IP_3 receptor : structure, function, and localization in oocvtes and eggs."Cell,. 73. 555-570 (1993)

2000 年度実績報告書

御子柴克彦東京大学, 医科学研究所, 教授 (30051840)