2001 年度実績報告書

皮膚樹状細胞の活性化・遊走シグナルの解析と修飾、およびその治療への応用

研究課題

研究課題/領域番号	11470183
研究機関	九州大学
研究代表者	古江増隆九州大学, 大学院・医学研究院, 教授 (70134583)
研究分担者	寺尾浩九州大学, 医学部・附属病院, 助手 (80274447)
キーワード	樹状細胞 / ランゲルハンス細胞 / サイトカイン / ケモカイン / 乾癬 / 真菌乾癬 / 抗酸化剤
研究概要	皮膚樹状細胞(ランゲルハンス細胞)の活性化と遊走シグナルは密に関連して合目的に制御されていると考えられる。11年度はハプテン外用後に生ずるランゲルハンス細胞の活性化の病態(J Invest Dermatol(Synp Proc)4:164,1999)、ランゲルハンス細胞の真皮から表皮内への移入におけるIL6とTNF-αの役割(J Dermatol Sci 19:166,1999)について明らかにした。12年度は、表皮内有棘細胞癌であるボーエン病におけるCD1a陽性樹状細胞と表皮内CD3,CD4,CD68各陽性細胞数との動態と腫瘍細胞との関連を明らかにした(Brit J Dermatol 143:1211,2000)。また抗酸化剤の一つであるCX-659Sがマウスならびにモルモットの接触皮膚炎のいくつかのモデルを抑制すること、局所のIL-1bやTNF-a産生を抑制することを明らかにした(Int. Arch.Anergy Immnol 123:341,2000)。現在、表皮細胞のGM-CSF産生とランゲルハンス細胞の活性化に対する抑制効果を検討している。さらに黒色腫におけるマクロファージの動態とTNF-a、IL-8、VEGFの産生の関連性を明らかにした(Int. J Cancer85,182,2000,Jap J Cancer Res91:906,2000)。13年度は、樹状細胞の場を形成する表皮細胞のケモカイン・サイトカインの動態とIL-13 receptorの局在を観察し(J Dermatol Sci 25:229-235,2001,Cytokine 13:75-84,2001,J Dermatol Sci, in press)、真菌感染症や乾癬におけるIFN-gやIL-8産生細胞の局在を明らかにした(Eur J Dermatol 11:105, 2001,J Demmtol Sci 26:119,2001,Eur J Demmtol, in press, Dermatology, in press)。また腫瘍消腿や真菌性肉芽腫、皮膚リンパ濾胞形成における樹状細胞の関与を指摘した(Dendriti Cells 11:13,2001,Clin Exp Dermatol 26:705,2001,Eur J Dermatol,11:527,2001)。

研究成果

(28件)

すべてその他

すべて文献書誌 (28件)

[文献書誌] M Furue, H Duan, H Uchi, et al.: "Multiple spontaneous regression of seborrheic keratosis associated with nasal carcinoma"Clin Exp Dermatol. (in press).
[文献書誌] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical localization of activated and mature CD83+ dendritic cells in granulomas of sprotrichosis"Eur J Dermatol. (in press).
[文献書誌] Koga T, Duan H, Urabe K, Furue M: "In situ localization of IFN-γ positive cells in psoriatic lesional epidermis"Eur J Dermatol. (in press).
[文献書誌] Koga T, Duan H, Urabe K, Furue M: "In situ localization of CD83-positive dendritic cells in psoriatic lesions"Dermatology. (in press).
[文献書誌] Kohda F, Koga T, Uchi H, Urabe K, Furue M: "Histamine-induced IL-6 and IL-8 production are differentially modulated by IFN-γ and IL-4 in human keratinocytes"J Dermatol Sci. (in press).
[文献書誌] Wakugawa M, Nakamura K, Tamaki K, Furue M et al.: "Expression of CC chemokine receptor on human keratinocytes in vivo and in vitro -upregulation by RANTES-"J Dermatol Sci. 25. 229-235 (2001)
[文献書誌] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical detection of interferon-γ -producing cells in dermatophytosis"Eur J Dermatol. 11. 105-107 (2001)
[文献書誌] Duan H, Koga T, Kohda F, Hara H, Urabe K, Furue M: "Interleukin-8-positive neutrophils in psoriasis"J Dermatol Sci. 26. 119-124 (2001)
[文献書誌] Akikawa M, Yu B, Umeshita-Suyama R, Furue M et al.: "Localization of interleukin 13 receptor in non-heamatopoietic cells"Cytokine. 13. 75-84 (2001)
[文献書誌] Yoshida Y, Duan H, Koga T, Furue M: "Immunohistochemical study on cutaneous lymphoid follicles in lymphadenosis benigna cutis, angiolymphoid hyperplasia with eosinophilia, and Kimura's disease in comparison with normal human lymph nodes"Dendriti Cells. 11. 13-16 (2001)
[文献書誌] Juichiro Nakayama, Hiroshi Terao, Tetsuya Koga, Masutaka Furue: "Induction of CD54and CD58 expression in cultured human endothelial cells by β-interferon with or without hyperthermia in vitro"Journal of Dermatological Science. 26. 19-24 (2001)
[文献書誌] Yoshinori Ohnishi, Katsuya Hirano, Junji Nishimura, Masutaka Furue, Hideo Kanaide: "Inhibitory effects of brefeldin A, a membrane transport blocker, on the bradykinin-induced hyperpolarization-mediated relaxation in the porcine coronary artery"British Journal of Pharmacology. 134. 168-178 (2001)
[文献書誌] H Duan, T Koga, T Masuda, H Kiryu, M Furue et al.: "CD1a-H, CD3+, CD4+, CDS+, CD68+ and CLA+ cells in Bowen's disease"Brit J Dermatol. 143. 1211-1216 (2000)
[文献書誌] Goto Y, Inoue Y, Tsuchida M, Isobe M, Ueno T, Uchi H, Furue M et al.: "Suppressive Effect of Topically Applied CX-659S, a Novel Diaminouracil Derivative, on the Contact Hypersensitivity Reaction in Various Animal Models"Int.Arch.Allergy Immunol. 123. 341-348 (2000)
[文献書誌] Hitoe TORISU, Mayumi ONO, Hiromaro KIRYU, Masutaka FURUE: "Macrophage infiltration correlates with tumor stage and angiogenesis in human malignant melanoma : Possible involvement of TNF α and IL-1 α"Int J Cancer. 85. 182-188 (2000)
[文献書誌] Hitoe TORISU-ITAKURA, Masutaka FURUE et al.: "Co-expression of tymidine phosphorylase and heme oxygenase-1 in macrophages in human malignant vertical growth melanomas"Jap J Cancer Res. 91. 906-910 (2000)
[文献書誌] Izumi Horiuchi, Yuji Kawano, Kenji Yamasaki, Motozumi minohara, Masutaka Furue et al.: "Th1 dominance in HAM/TSP and the optico-spinal form of multiple sclerosis versus Th2 dominance in mite antigen-specific IgE myelitis"J Neurol Sci. 172. 17-24 (2000)
[文献書誌] Wakugawa M, Nakamura K, Hino H, Furue M et al.: "Elevated levels of eotaxin and interleukin-5 in blister fluid of bullous pemphigoid : correlation with eosinophilia"Brit J Dermatol. 143. 112-116 (2000)
[文献書誌] Yasunobu Abe, Koichiro Muta, Koichi Oshima, Masutaka Furue, et al.: "Subcutaneous panniculitis by Epstein-Barr virus-infected natural killer (NK) cell proliferation terminating in aggressive subcutaneous NK cell lymphma"Am J Hematol. 64. 221-225 (2000)
[文献書誌] Araki M, Imafuku S, Furue M et al.: "Activation pattern of Langerhans cells in the afferent and efferent phases of contact hypersensitivity"J Invest Dermatol (Synp Proc). 4. 164-168 (1999)
[文献書誌] Saitoh A, Yasaka N, Osada A, Nakamura K, Furue M et al.: "Migration of Langerhans cells in an in vitro organ culture system : IL-6 and TNF-a are partially responsible for migration Into the epidermis"J Dermatol Sci. 19. 166-174 (1999)
[文献書誌] Duan H, Imafuku S, Sueishi K, Hori Y, Furue M et al.: "The proliferative properties of tumor cells differentially correlate with the host immune responses in anogenital Bowen's disease"J Dermatol Sci. 20. 53-62 (1999)
[文献書誌] Furue M, Koga T, Yamashita N: "Soluble E-selectin and eosinophil cationic protein are distinct serum markers that differentially represent clinical features of atopic dermatitis"Brit J Dermatol. 140. 67-72 (1999)
[文献書誌] Kira J, Kawano Y, Furue M et al.: "Clinical, immunological and MRL features of myelitis with atopic dermatitis (atopic myelitis)"J Neurol Sci. 162. 56-61 (1999)
[文献書誌] Toshitani A, Yoshinaga T, Furue M: "Reduced amount of secretory component of IgA secretion in tears of patients with atopic dermatitis"J Dermatol Sci. 19. 134-138 (1999)
[文献書誌] Nakayarna J, Kiryu H, Urabe K, Matsuo S, Koga T, Furue M: "Vitamin D3 analogues improve cafe-au lait spots in patients with von Recklinghausen's disease : experimental and clinical studies"Eur J Dermatol. 9. 202-206 (1999)
[文献書誌] Jun Matsunaga, Miwako Dakeishi-Hara, Muneo Tanita, Kanako Kikuchi, Masutaka Furue et al.: "A splicing mutation of the tyrosinase gene causes yellow oculocutaneous albinism in a Japanese patient with a pigmented phenotype"Dermatology. 199. 124-129 (1999)
[文献書誌] Katsunori Fujii, Toshiyuki Miyashita, Jun-ichi Takahashi, Katsuo Sugita, Masutaka Furue: "Gamma-irradiarion deregulates cell cycle control and apoptosis in nevoid basal cell carcinoma syndrome-derived cells"Jpn J Cancer Res. 90. 1351-1357 (1999)

2001 年度 実績報告書

皮膚樹状細胞の活性化・遊走シグナルの解析と修飾、およびその治療への応用

研究代表者

古江 増隆 九州大学, 大学院・医学研究院, 教授 (70134583)

研究成果

[文献書誌] M Furue, H Duan, H Uchi, et al.: "Multiple spontaneous regression of seborrheic keratosis associated with nasal carcinoma"Clin Exp Dermatol. (in press).

[文献書誌] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical localization of activated and mature CD83+ dendritic cells in granulomas of sprotrichosis"Eur J Dermatol. (in press).

[文献書誌] Koga T, Duan H, Urabe K, Furue M: "In situ localization of IFN-γ positive cells in psoriatic lesional epidermis"Eur J Dermatol. (in press).

[文献書誌] Koga T, Duan H, Urabe K, Furue M: "In situ localization of CD83-positive dendritic cells in psoriatic lesions"Dermatology. (in press).

[文献書誌] Kohda F, Koga T, Uchi H, Urabe K, Furue M: "Histamine-induced IL-6 and IL-8 production are differentially modulated by IFN-γ and IL-4 in human keratinocytes"J Dermatol Sci. (in press).

[文献書誌] Wakugawa M, Nakamura K, Tamaki K, Furue M et al.: "Expression of CC chemokine receptor on human keratinocytes in vivo and in vitro -upregulation by RANTES-"J Dermatol Sci. 25. 229-235 (2001)

[文献書誌] Koga T, Duan H, Urabe K, Furue M: "Immunohistochemical detection of interferon-γ -producing cells in dermatophytosis"Eur J Dermatol. 11. 105-107 (2001)

[文献書誌] Duan H, Koga T, Kohda F, Hara H, Urabe K, Furue M: "Interleukin-8-positive neutrophils in psoriasis"J Dermatol Sci. 26. 119-124 (2001)

[文献書誌] Akikawa M, Yu B, Umeshita-Suyama R, Furue M et al.: "Localization of interleukin 13 receptor in non-heamatopoietic cells"Cytokine. 13. 75-84 (2001)

[文献書誌] Yoshida Y, Duan H, Koga T, Furue M: "Immunohistochemical study on cutaneous lymphoid follicles in lymphadenosis benigna cutis, angiolymphoid hyperplasia with eosinophilia, and Kimura's disease in comparison with normal human lymph nodes"Dendriti Cells. 11. 13-16 (2001)

[文献書誌] Juichiro Nakayama, Hiroshi Terao, Tetsuya Koga, Masutaka Furue: "Induction of CD54and CD58 expression in cultured human endothelial cells by β-interferon with or without hyperthermia in vitro"Journal of Dermatological Science. 26. 19-24 (2001)

[文献書誌] H Duan, T Koga, T Masuda, H Kiryu, M Furue et al.: "CD1a-H, CD3+, CD4+, CDS+, CD68+ and CLA+ cells in Bowen's disease"Brit J Dermatol. 143. 1211-1216 (2000)

[文献書誌] Goto Y, Inoue Y, Tsuchida M, Isobe M, Ueno T, Uchi H, Furue M et al.: "Suppressive Effect of Topically Applied CX-659S, a Novel Diaminouracil Derivative, on the Contact Hypersensitivity Reaction in Various Animal Models"Int.Arch.Allergy Immunol. 123. 341-348 (2000)

[文献書誌] Hitoe TORISU, Mayumi ONO, Hiromaro KIRYU, Masutaka FURUE: "Macrophage infiltration correlates with tumor stage and angiogenesis in human malignant melanoma : Possible involvement of TNF α and IL-1 α"Int J Cancer. 85. 182-188 (2000)

[文献書誌] Hitoe TORISU-ITAKURA, Masutaka FURUE et al.: "Co-expression of tymidine phosphorylase and heme oxygenase-1 in macrophages in human malignant vertical growth melanomas"Jap J Cancer Res. 91. 906-910 (2000)

[文献書誌] Izumi Horiuchi, Yuji Kawano, Kenji Yamasaki, Motozumi minohara, Masutaka Furue et al.: "Th1 dominance in HAM/TSP and the optico-spinal form of multiple sclerosis versus Th2 dominance in mite antigen-specific IgE myelitis"J Neurol Sci. 172. 17-24 (2000)

[文献書誌] Wakugawa M, Nakamura K, Hino H, Furue M et al.: "Elevated levels of eotaxin and interleukin-5 in blister fluid of bullous pemphigoid : correlation with eosinophilia"Brit J Dermatol. 143. 112-116 (2000)

[文献書誌] Yasunobu Abe, Koichiro Muta, Koichi Oshima, Masutaka Furue, et al.: "Subcutaneous panniculitis by Epstein-Barr virus-infected natural killer (NK) cell proliferation terminating in aggressive subcutaneous NK cell lymphma"Am J Hematol. 64. 221-225 (2000)

[文献書誌] Araki M, Imafuku S, Furue M et al.: "Activation pattern of Langerhans cells in the afferent and efferent phases of contact hypersensitivity"J Invest Dermatol (Synp Proc). 4. 164-168 (1999)

[文献書誌] Saitoh A, Yasaka N, Osada A, Nakamura K, Furue M et al.: "Migration of Langerhans cells in an in vitro organ culture system : IL-6 and TNF-a are partially responsible for migration Into the epidermis"J Dermatol Sci. 19. 166-174 (1999)

[文献書誌] Duan H, Imafuku S, Sueishi K, Hori Y, Furue M et al.: "The proliferative properties of tumor cells differentially correlate with the host immune responses in anogenital Bowen's disease"J Dermatol Sci. 20. 53-62 (1999)

[文献書誌] Furue M, Koga T, Yamashita N: "Soluble E-selectin and eosinophil cationic protein are distinct serum markers that differentially represent clinical features of atopic dermatitis"Brit J Dermatol. 140. 67-72 (1999)

[文献書誌] Kira J, Kawano Y, Furue M et al.: "Clinical, immunological and MRL features of myelitis with atopic dermatitis (atopic myelitis)"J Neurol Sci. 162. 56-61 (1999)

[文献書誌] Toshitani A, Yoshinaga T, Furue M: "Reduced amount of secretory component of IgA secretion in tears of patients with atopic dermatitis"J Dermatol Sci. 19. 134-138 (1999)

[文献書誌] Nakayarna J, Kiryu H, Urabe K, Matsuo S, Koga T, Furue M: "Vitamin D3 analogues improve cafe-au lait spots in patients with von Recklinghausen's disease : experimental and clinical studies"Eur J Dermatol. 9. 202-206 (1999)

[文献書誌] Jun Matsunaga, Miwako Dakeishi-Hara, Muneo Tanita, Kanako Kikuchi, Masutaka Furue et al.: "A splicing mutation of the tyrosinase gene causes yellow oculocutaneous albinism in a Japanese patient with a pigmented phenotype"Dermatology. 199. 124-129 (1999)

[文献書誌] Katsunori Fujii, Toshiyuki Miyashita, Jun-ichi Takahashi, Katsuo Sugita, Masutaka Furue: "Gamma-irradiarion deregulates cell cycle control and apoptosis in nevoid basal cell carcinoma syndrome-derived cells"Jpn J Cancer Res. 90. 1351-1357 (1999)

2001 年度実績報告書

古江増隆九州大学, 大学院・医学研究院, 教授 (70134583)