2001 年度実績報告書

ウナギ属魚類の起源と進化に関する研究

研究課題

研究課題/領域番号	11691177
研究機関	東京大学
研究代表者	塚本勝巳東京大学, 海洋研究所, 教授 (10090474)
研究分担者	小川和夫東京大学, 農学生命科学研究科, 教授 (20092174) 金子豊二東京大学, 海洋研究所, 助教授 (70221190) 西田睦東京大学, 海洋研究所, 教授 (90136896) 青山潤東京大学, 海洋研究所, 助手大竹ニ雄三重大学, 生物資源学部, 教授 (20160525)
キーワード	ウナギ / 回遊 / 進化 / 適応機構 / 分子系統 / 塩類細胞 / 耳石 / mtDNA
研究概要	本年度は以下の3項目について計3ヶ国で8回の海外調査を実施し,次の結果を得た。 1.熱帯ウナギの回遊生態:インドネシア・スラウェシ島一周航海を行い,計2,592尾のレプトケファルスを得た。この内,ウナギ属は53尾でAnguilla interioris, A. bornensis, A. celebesensis, A. marmorata, A. bicolor pacificaの計5種が採集された。仔魚の分布とサイズの解析からA. bornencess とA. celebecensisの産卵場がそれぞれ,セレベス海とトミニ湾にあることが明らかになった。2.大西洋ウナギの種分化:北アメリカに分布するA.rostrataとヨーロッパのA.anguillaが同所的に分布するアイスランドにおいてシラスウナギの採集調査を実施したところ,これら2種の接岸日齢はともに約1年と等しく,サルガッソー海で孵化した両種がほぼ同様の輸送過程を経てアイスランドに加入するものと考えられた。これら大西洋群2種が分化したのは両者の変態時期のズレが原因で,分子系統解析より,それは今から約100万年前と推定された。3.ウナギと寄生虫の共進化:リボゾームRNA遺伝子のITS2領域を用いて解析したところ,汽水域の天然ウナギ鰓弁に寄生する新種,Pseudodactylogyrus kamegaiiは淡水域のウナギに寄生するP.biniやP.anguillaeより早く分岐していたことから、Pseudodactylogyrusは本来海起源で,宿主の淡水への適応進化に伴って種分化したことが示唆された。この他,本研究最終年の今年度は,研究の総まとめとして2001年9月に国際シンポジウム「Advances in Eel Biology」を東京大学において開催し,計79件の研究発表を行った。また,計22編の原著論文,3編の総説なども出版した。

研究成果
(29件)

すべてその他

すべて文献書誌 (29件)

[文献書誌] SASAI, Seiji et al.: "Occurrence of migrating silver eels Anguilla japonica in the East China Sea"Marine Ecology of Progress Series. 212. 305-310 (2001)
[文献書誌] ISHIKAWA, Satoshi et al.: "Population structure of the Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing"Fiseries Science. 67. 246-253 (2001)
[文献書誌] 塚本勝巳: "ウナギの産卵生態"海洋と生物. 133. 123-129 (2001)
[文献書誌] 大竹二雄: "ウナギの初期生活史"海洋と生物. 133. 137-145 (2001)
[文献書誌] 青山潤: "ウナギの分子系統"海洋と生物. 133. 146-152 (2001)
[文献書誌] INOUE, Jun et al.: "Complete Mitochondrial DNA Sequence of the Japanese Eel, Anguilla japonica"Fisheries Science. 67. 118-125 (2001)
[文献書誌] ARAI, Takaomi et al.: "Early life history of tropical Angilla leptocephali in the Western Pacific Ocean"Marino Biology. 138. 887-895 (2001)
[文献書誌] MARUI, Miho et al.: "Comparison of early life history between New Zealand temperate eels and Pacific tropical eels revealed by otolith microsructure and microchemistry"Marine Ecology of Progress Series. 213. 273-284 (2001)
[文献書誌] ARAI, Takaomi et al.: "Recruitment mechanisms ot tropical eels Anguilla spp. and implications for the evolutn of oceanic migration in the genus Anguilla"Marina Ecology Progress Series. 216. 253-264 (2001)
[文献書誌] AOYAMA, Jun et al.: "Molecular approach for species identification of eggs with respect to determination of the spawning site of the Japanese eel Anguilla japonica"Fisheries Science. 67. 761-763 (2001)
[文献書誌] AOYAMA, Jun et al.: "Molecular phylogeny and evolution of the freshwater eel, genus Anguilla"Molecular Phylogenetics and Evolution. 20・3. 450-459 (2001)
[文献書誌] TSUKAMOTO, Katsumi, Takaomi ARAI: "Facultative catadromy of the eel, Anguilla japonica, between freshwater and seawater habitats"Marine Ecology of Progress Series. 220. 265-276 (2001)
[文献書誌] SUGEHA, Hagi, Yulia et al.: "Validation of otolith daily increments in the tropical eel Ariguilla marmorata"Marine Ecology Progress Series. 220. 291-294 (2001)
[文献書誌] SUGEHA, Hagi, Yulia et al.: "Inshore migration of the tropical eels, Anguilla spp. recruiting to the Poigar River estuary on north Sulawesi Island"Marine Ecology Progress Series. 221. 233-243 (2001)
[文献書誌] ISHIKAWA, Satoshi et al.: "Spawning time and place of the Japanese eel Anguilla japonica in the North Equatorial Current of the western North Pacific Ocean"Fisheries Science. 67. 1097-1103 (2001)
[文献書誌] Hayward, C.J. et al.: "First report of the invasive eel pest, Pseudodactylogyrus bini, in North America and in wild American eels"Diseases of Aquatic Organisms. 44・1. 53-60 (2001)
[文献書誌] ARAI, Takaomi et al.: "Inshore migration of a tropical eel, Anguilla marmorata, from Taiwanese and Japanese coasts"Fisheries Science. 68. 152-157 (2002)
[文献書誌] MILLER, Michael J., Katsumi TSUKAMOTO: "Evidence of a spawning area of Anguilla marmorata in the North Equatorial Current of the Western North Pacific"Journal of Taiwan Fisheries Research. (in press). (2002)
[文献書誌] AOYAMA, Jun et al.: "The first tracking studies on yellow and silver eels, Anguilla japonica, in the estuarine habitat"Journal of Taiwan Fisheries Research. (in press). (2002)
[文献書誌] ISHIKAWA, Satoshi et al.: "Utility of microsatellite markers for eel study"Journal of Taiwan Fisheries Research. (in press). (2002)
[文献書誌] ISHIKAWA, Satoshi et al.: "Characterization of microsatellite loci from the Japanese eel Anguilla japonica"Molecular Ecology Notes. (in press). (2002)
[文献書誌] MILLER, Michael J. et al.: "Evidence of a spawning area of Anguilla marmorata in the western North Pacific"Marine Biology. (in press). (2002)
[文献書誌] MILLER, Michael J. et al.: "Distribution of leptocephali in the Kuroshia Current and East China Sea"Marine Ecology of Progress Series. (in press). (2002)
[文献書誌] ARAI, Takaomi et al.: "Growth history and inshore migration of the tropical eel, Anguilla marmorata, in the Pacific"Marine Biology. (in press). (2002)
[文献書誌] AOYAMA, Jun et al.: "Preliminary study on the movements of yellow and silver eels in the river mouth of the Fukui River, Japan, revealed by accoustic tracking"Hydrobiologia. (in press). (2002)
[文献書誌] TSUKAMOTO Katsumi et al.: "Spawning ground of the Japanese eel"Springer-Verlag (Eel Biology)(in press). (2002)
[文献書誌] NISHIDA Mutsumi: "Population structure and speciation in the Pacific eels"Springer-Verlag (Eel Biology)(in press). (2002)
[文献書誌] OTAKE Tsuguo: "Metamorphosis"Springer-Verlag (Eel Biology)(in press). (2002)
[文献書誌] AOYAMA Jun: "Origin and evolution of Anguillid eels"Springer-Verlag (Eel Biology)(in press). (2002)

2001 年度 実績報告書

ウナギ属魚類の起源と進化に関する研究

研究代表者

塚本 勝巳 東京大学, 海洋研究所, 教授 (10090474)

研究成果

[文献書誌] SASAI, Seiji et al.: "Occurrence of migrating silver eels Anguilla japonica in the East China Sea"Marine Ecology of Progress Series. 212. 305-310 (2001)

[文献書誌] ISHIKAWA, Satoshi et al.: "Population structure of the Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing"Fiseries Science. 67. 246-253 (2001)

[文献書誌] 塚本勝巳: "ウナギの産卵生態"海洋と生物. 133. 123-129 (2001)

[文献書誌] 大竹二雄: "ウナギの初期生活史"海洋と生物. 133. 137-145 (2001)

[文献書誌] 青山潤: "ウナギの分子系統"海洋と生物. 133. 146-152 (2001)

[文献書誌] INOUE, Jun et al.: "Complete Mitochondrial DNA Sequence of the Japanese Eel, Anguilla japonica"Fisheries Science. 67. 118-125 (2001)

[文献書誌] ARAI, Takaomi et al.: "Early life history of tropical Angilla leptocephali in the Western Pacific Ocean"Marino Biology. 138. 887-895 (2001)

[文献書誌] MARUI, Miho et al.: "Comparison of early life history between New Zealand temperate eels and Pacific tropical eels revealed by otolith microsructure and microchemistry"Marine Ecology of Progress Series. 213. 273-284 (2001)

[文献書誌] ARAI, Takaomi et al.: "Recruitment mechanisms ot tropical eels Anguilla spp. and implications for the evolutn of oceanic migration in the genus Anguilla"Marina Ecology Progress Series. 216. 253-264 (2001)

[文献書誌] AOYAMA, Jun et al.: "Molecular approach for species identification of eggs with respect to determination of the spawning site of the Japanese eel Anguilla japonica"Fisheries Science. 67. 761-763 (2001)

[文献書誌] AOYAMA, Jun et al.: "Molecular phylogeny and evolution of the freshwater eel, genus Anguilla"Molecular Phylogenetics and Evolution. 20・3. 450-459 (2001)

[文献書誌] TSUKAMOTO, Katsumi, Takaomi ARAI: "Facultative catadromy of the eel, Anguilla japonica, between freshwater and seawater habitats"Marine Ecology of Progress Series. 220. 265-276 (2001)

[文献書誌] SUGEHA, Hagi, Yulia et al.: "Validation of otolith daily increments in the tropical eel Ariguilla marmorata"Marine Ecology Progress Series. 220. 291-294 (2001)

[文献書誌] SUGEHA, Hagi, Yulia et al.: "Inshore migration of the tropical eels, Anguilla spp. recruiting to the Poigar River estuary on north Sulawesi Island"Marine Ecology Progress Series. 221. 233-243 (2001)

[文献書誌] ISHIKAWA, Satoshi et al.: "Spawning time and place of the Japanese eel Anguilla japonica in the North Equatorial Current of the western North Pacific Ocean"Fisheries Science. 67. 1097-1103 (2001)

[文献書誌] Hayward, C.J. et al.: "First report of the invasive eel pest, Pseudodactylogyrus bini, in North America and in wild American eels"Diseases of Aquatic Organisms. 44・1. 53-60 (2001)

[文献書誌] ARAI, Takaomi et al.: "Inshore migration of a tropical eel, Anguilla marmorata, from Taiwanese and Japanese coasts"Fisheries Science. 68. 152-157 (2002)

[文献書誌] MILLER, Michael J., Katsumi TSUKAMOTO: "Evidence of a spawning area of Anguilla marmorata in the North Equatorial Current of the Western North Pacific"Journal of Taiwan Fisheries Research. (in press). (2002)

[文献書誌] AOYAMA, Jun et al.: "The first tracking studies on yellow and silver eels, Anguilla japonica, in the estuarine habitat"Journal of Taiwan Fisheries Research. (in press). (2002)

[文献書誌] ISHIKAWA, Satoshi et al.: "Utility of microsatellite markers for eel study"Journal of Taiwan Fisheries Research. (in press). (2002)

[文献書誌] ISHIKAWA, Satoshi et al.: "Characterization of microsatellite loci from the Japanese eel Anguilla japonica"Molecular Ecology Notes. (in press). (2002)

[文献書誌] MILLER, Michael J. et al.: "Evidence of a spawning area of Anguilla marmorata in the western North Pacific"Marine Biology. (in press). (2002)

[文献書誌] MILLER, Michael J. et al.: "Distribution of leptocephali in the Kuroshia Current and East China Sea"Marine Ecology of Progress Series. (in press). (2002)

[文献書誌] ARAI, Takaomi et al.: "Growth history and inshore migration of the tropical eel, Anguilla marmorata, in the Pacific"Marine Biology. (in press). (2002)

[文献書誌] AOYAMA, Jun et al.: "Preliminary study on the movements of yellow and silver eels in the river mouth of the Fukui River, Japan, revealed by accoustic tracking"Hydrobiologia. (in press). (2002)

[文献書誌] TSUKAMOTO Katsumi et al.: "Spawning ground of the Japanese eel"Springer-Verlag (Eel Biology)(in press). (2002)

[文献書誌] NISHIDA Mutsumi: "Population structure and speciation in the Pacific eels"Springer-Verlag (Eel Biology)(in press). (2002)

[文献書誌] OTAKE Tsuguo: "Metamorphosis"Springer-Verlag (Eel Biology)(in press). (2002)

[文献書誌] AOYAMA Jun: "Origin and evolution of Anguillid eels"Springer-Verlag (Eel Biology)(in press). (2002)

2001 年度実績報告書

塚本勝巳東京大学, 海洋研究所, 教授 (10090474)