2000 年度実績報告書

魚類のミトコンドリア・ゲノミクス:mtDNA全塩基配列決定に基づく解析

研究課題

研究課題/領域番号	12460083
研究機関	東京大学
研究代表者	西田睦東京大学, 海洋研究所, 教授 (90136896)
研究分担者	熊澤慶伯名古屋大学, 大学院・理学研究科, 講師 (60221941)
キーワード	ミトコンドリア DNA / 魚類 / 分子進化 / 集団構造 / 系統解析 / ミトコンドリアゲノミクス
研究概要	核ゲノムに比べてサイズ的にもコンパクトなミトコンドリアゲノム(mtゲノム)は,動物の集団構造や系統のDNAレベルでの解析のための第1次的な分析対象として注目されている。しかし,魚類のmtゲノムに関する知見はまだ非常に限られているため,遺伝マーカーとしての有用性がまだ十分活かせない状況にある。そこで,1)魚類の主要なグループを代表する約30種について,mtゲノムの全塩基配列を決定し,2)その結果を詳細に比較分析することにより,魚類のミトコンドリアゲノムの実態と進化動態を明らかにすることを目的に本研究を開始した。われわれ自身が最近開発した効率的手法を活用した結果,今年度の目標分析種数(30種)のmtゲノムの全塩基配列を決定することができた。とくに今年度は,現生魚類の大部分を占め,かつ有用水産資源の過半を占める真骨類を中心に取り上げたが,中でも"低位"魚類に力点をおいて研究を進めた。また,得られた塩基配列データの解析を行っているが,脊椎動物で初めて見出された遺伝子配置の変動がいくつか見つかった。さらに,塩基配列データを基に,予備的な分子系統学的解析を行った結果,ウナギなどが属するカライワシ類が従来想定されていたよりも系統的により"低位"に位置することなど,新たな知見が得られた。以上のように,魚類のミトコンドリアゲノムの実態と進化動態の解明に資する情報の蓄積が着実に進んでいる。

研究成果
(24件)

すべてその他

すべて文献書誌 (24件)

[文献書誌] Takahashi,Kazuhiko: "Retroposition of the AFC family of SINEs before and during the adaptive radiation of cichlid fishes in Lake Malawi and their usage for phylogenetic inference."Journal of Molecular Evolution. (in press). (2001)
[文献書誌] Inoue,Jun G.: "A mitogenomic perspective on the basal teleostean phylogeny : resolving higher-level relationships with longer DNA sequences."Molecular Phylogenetics and Evolution. (in press). (2001)
[文献書誌] Ishikawa,Satoshi: "Charactarization of microsatellite loci from the Japanese eel Anguilla japonica."Molecular Ecology Notes. (in press). (2001)
[文献書誌] Suzuki,Nobuaki: "The phylogenetic position of the genus Atheresthes (Pleuronectidae) and its classification : A molecular phylogenetic approach using mitochondrial sequence data."Bulletin of Faculty of Fisheries Hokkaido University. (in press). (2001)
[文献書誌] Kawaguchi,Akira: "Complete mitochondrial DNA Sequence of Aulopus japonicus (Teleostei : Anlopiformes), a basal Eurypterygii : Longer DNA sequences and higher-level relationships."Ichthyological Research. (in press). (2001)
[文献書誌] Ishiguro,Naoya: "Complete mitochondrial DNA sequence of ayu, Plecoglossus altivelis"Fisheries Science. (in press). (2001)
[文献書誌] Inoue,Jun G.: "The Complete mitochondrial DNA sequences of the Japanese archory, Engraulis japonicus."Fisheries Science. (in press). (2001)
[文献書誌] Inoue,Jun G.: "Complete mitochondrial DNA sequence of Longer miriaster : Novel gene order for vertebrate mitochondrial genoms and the phylogenetics implications for Anguilli : form families."Journal of Molecular Evolution. (in press). (2001)
[文献書誌] Watanabe,Katsutoshi: "Isolation and characterization of microsatellite loci from the endangered bagrid catfish, Pseudobagrus ichikawai."Molecular Ecology Notes. (in press). (2001)
[文献書誌] Ishikawa,Satoshi: "Population structure of Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing."Fisheries Science. (in press). (2001)
[文献書誌] Inoue,Jun G.: "The complete mitochondrial DNA sequence of Japanese eel Anguilla japonica."Fisheries Science. (in press). (2001)
[文献書誌] Kecichi Matsuura: "Fishes from the inside moats of the Imperial Palace, Tokyo, Japan."Memoir of the National Science Museum, Tokyo. 35. 57-68 (2000)
[文献書誌] Aoyama,Jun: "The European eel, Anguilla anguilla (L.), in Japanese waters."Dana. 12. 1-5 (2000)
[文献書誌] Iguchi,Kei-ichiro: "Genetic biogeography among insular populations of the amphidromous fish Plecoglossus altivelis assessed from mitochondrial DNA analysis."Conservation Genetics. 1. 147-156 (2000)
[文献書誌] Kumazawa,Yoshinori: "Molecular phylogeny of osteogrossoids : a new model for Gondwanian origin and plate tectonic transportation of the Asia arowana."Molecular Biology and Evolution. 17. 1869-1878 (2000)
[文献書誌] Miya,Masaki: "Use of mitogenomic information in teleostean molecular phylogenetics. A tree-based exploration under the maximum-parsimory optimality criterion."Molecular phylogenetics and Evolution. 17. 437-455 (2000)
[文献書誌] Ishikawa,Satoshi: "Gene rearrangement around the control region in the mitochondrial genome of conger eel Conger myriaster."Fisheries Science. 66. 1187-1190 (2000)
[文献書誌] Inoue,Jun G.: "Complete mitochondrial DNA sequence of the Japanese sardine, Sardinops melanostictus."Fisheries Science. 66. 924-932 (2000)
[文献書誌] Aoyama,Jun: "Discrimination of catadromous eels of genus Anguilla using polymerase chain reaction-restriction fragment length polymorphism analysis of the mitochondrial 16S ribosomal RNA domain."Transaction of the American Fisheries Society. 129. 873-878 (2000)
[文献書誌] Aoyama,Jun: "Are morphological characters distinctive enough to discriminate between two species of freshwater eels Anguilla celebesensis and A.interioris?"Ichthyological Research. 47. 157-161 (2000)
[文献書誌] Yamaguchi,Motoomi: "Molecular phylogeny and larval morphological diversity of the lanternfish genus Hygophum."Molecular Phylogenetics and Evolution. 15. 103-114 (2000)
[文献書誌] Miya,Masaki: "Molecular systematis of the deep-sea fish genus Gonostoma : two paraphyletic clades and resurrection of Sigmops."Copeia. 2000. 378-389 (2000)
[文献書誌] Watanabe,Katsutoshi: "Phylogenetic relationships of the Japanese minnows, Pseudorasbora, as inferred from mitochondrial 16 SrRNA gene sequences."Ichthyological Research. 47. 43-50 (2000)
[文献書誌] Kumazawa,Yoshinori: "Molecular phylogenetic analysis of vertebrate radiations. in IIAS Reports (ed.K.Iwatsuki)"International Institute for Advanced Studies, Kyoto. (2000)

2000 年度 実績報告書

魚類のミトコンドリア・ゲノミクス:mtDNA全塩基配列決定に基づく解析

研究代表者

西田 睦 東京大学, 海洋研究所, 教授 (90136896)

研究成果

[文献書誌] Takahashi,Kazuhiko: "Retroposition of the AFC family of SINEs before and during the adaptive radiation of cichlid fishes in Lake Malawi and their usage for phylogenetic inference."Journal of Molecular Evolution. (in press). (2001)

[文献書誌] Inoue,Jun G.: "A mitogenomic perspective on the basal teleostean phylogeny : resolving higher-level relationships with longer DNA sequences."Molecular Phylogenetics and Evolution. (in press). (2001)

[文献書誌] Ishikawa,Satoshi: "Charactarization of microsatellite loci from the Japanese eel Anguilla japonica."Molecular Ecology Notes. (in press). (2001)

[文献書誌] Suzuki,Nobuaki: "The phylogenetic position of the genus Atheresthes (Pleuronectidae) and its classification : A molecular phylogenetic approach using mitochondrial sequence data."Bulletin of Faculty of Fisheries Hokkaido University. (in press). (2001)

[文献書誌] Kawaguchi,Akira: "Complete mitochondrial DNA Sequence of Aulopus japonicus (Teleostei : Anlopiformes), a basal Eurypterygii : Longer DNA sequences and higher-level relationships."Ichthyological Research. (in press). (2001)

[文献書誌] Ishiguro,Naoya: "Complete mitochondrial DNA sequence of ayu, Plecoglossus altivelis"Fisheries Science. (in press). (2001)

[文献書誌] Inoue,Jun G.: "The Complete mitochondrial DNA sequences of the Japanese archory, Engraulis japonicus."Fisheries Science. (in press). (2001)

[文献書誌] Inoue,Jun G.: "Complete mitochondrial DNA sequence of Longer miriaster : Novel gene order for vertebrate mitochondrial genoms and the phylogenetics implications for Anguilli : form families."Journal of Molecular Evolution. (in press). (2001)

[文献書誌] Watanabe,Katsutoshi: "Isolation and characterization of microsatellite loci from the endangered bagrid catfish, Pseudobagrus ichikawai."Molecular Ecology Notes. (in press). (2001)

[文献書誌] Ishikawa,Satoshi: "Population structure of Japanese eel Anguilla japonica as examined by mitochondrial DNA sequencing."Fisheries Science. (in press). (2001)

[文献書誌] Inoue,Jun G.: "The complete mitochondrial DNA sequence of Japanese eel Anguilla japonica."Fisheries Science. (in press). (2001)

[文献書誌] Kecichi Matsuura: "Fishes from the inside moats of the Imperial Palace, Tokyo, Japan."Memoir of the National Science Museum, Tokyo. 35. 57-68 (2000)

[文献書誌] Aoyama,Jun: "The European eel, Anguilla anguilla (L.), in Japanese waters."Dana. 12. 1-5 (2000)

[文献書誌] Iguchi,Kei-ichiro: "Genetic biogeography among insular populations of the amphidromous fish Plecoglossus altivelis assessed from mitochondrial DNA analysis."Conservation Genetics. 1. 147-156 (2000)

[文献書誌] Kumazawa,Yoshinori: "Molecular phylogeny of osteogrossoids : a new model for Gondwanian origin and plate tectonic transportation of the Asia arowana."Molecular Biology and Evolution. 17. 1869-1878 (2000)

[文献書誌] Miya,Masaki: "Use of mitogenomic information in teleostean molecular phylogenetics. A tree-based exploration under the maximum-parsimory optimality criterion."Molecular phylogenetics and Evolution. 17. 437-455 (2000)

[文献書誌] Ishikawa,Satoshi: "Gene rearrangement around the control region in the mitochondrial genome of conger eel Conger myriaster."Fisheries Science. 66. 1187-1190 (2000)

[文献書誌] Inoue,Jun G.: "Complete mitochondrial DNA sequence of the Japanese sardine, Sardinops melanostictus."Fisheries Science. 66. 924-932 (2000)

[文献書誌] Aoyama,Jun: "Discrimination of catadromous eels of genus Anguilla using polymerase chain reaction-restriction fragment length polymorphism analysis of the mitochondrial 16S ribosomal RNA domain."Transaction of the American Fisheries Society. 129. 873-878 (2000)

[文献書誌] Aoyama,Jun: "Are morphological characters distinctive enough to discriminate between two species of freshwater eels Anguilla celebesensis and A.interioris?"Ichthyological Research. 47. 157-161 (2000)

[文献書誌] Yamaguchi,Motoomi: "Molecular phylogeny and larval morphological diversity of the lanternfish genus Hygophum."Molecular Phylogenetics and Evolution. 15. 103-114 (2000)

[文献書誌] Miya,Masaki: "Molecular systematis of the deep-sea fish genus Gonostoma : two paraphyletic clades and resurrection of Sigmops."Copeia. 2000. 378-389 (2000)

[文献書誌] Watanabe,Katsutoshi: "Phylogenetic relationships of the Japanese minnows, Pseudorasbora, as inferred from mitochondrial 16 SrRNA gene sequences."Ichthyological Research. 47. 43-50 (2000)

[文献書誌] Kumazawa,Yoshinori: "Molecular phylogenetic analysis of vertebrate radiations. in IIAS Reports (ed.K.Iwatsuki)"International Institute for Advanced Studies, Kyoto. (2000)

2000 年度実績報告書

西田睦東京大学, 海洋研究所, 教授 (90136896)