2001 年度実績報告書

体外肝組織再構築モデルを用いた胆汁うっ滞解析と細胞移植による肝不全治療法の研究

研究課題

研究課題/領域番号	12470265
研究機関	札幌医科大学
研究代表者	平田公一札幌医科大学, 医学部, 教授 (50136959)
研究分担者	桂巻正札幌医科大学, 医学部, 助教授 (50253993) 三高俊広札幌医科大学, 医学部, 教授 (50231618) 望月洋一札幌医科大学, 附属がん研究所, 教授 (40045381) 本間敏男札幌医科大学, 医学部, 助手 (30315494) 古畑智久札幌医科大学, 医学部, 助手
キーワード	小型肝細胞 / 分化能 / 冷凍保存 / 肝細胞形質 / 正常肝細胞
研究概要	札幌医科大学付属病院において、肝疾患のため肝切除が必要とされる症例の中で肝硬変症、ウイルス感染肝炎のいずれをも認めず切除された、正常肝組織部分からの肝組織採取が可能な症例において、肝切除手術時に腫瘍と共に切除される正常肝組織から一部を採取し、これを研究に利用することについて、札幌医科大学の「倫理委員会」の許可を得た。その後、肝組織片にコラゲナーゼ灌流法を用いて肝細胞を採取する研究を行っているが、couplet細胞、small hepatocyteの分離は繰り返し成されるが、研究を進展させるviabilityを得る段階にない。なぜなら、これらの細胞については採取され得るが、目的の期間の培養に耐えられない状況にあるのが実情である。一方、ラットの肝臓からsmall hepatocyteを採取、培養し、colonyまで増殖させても肝細胞の分化能を維持すること、および冷凍保存していた類肝細胞の形質変化について、培養後の組織学的検討(免疫染色・in situ hybridization・免疫電顕)、肝特異的酵素・タンパク質のmRNA・タンパク質の発現(Northern blot法、Western blot法)などについて確認することはできた。次年度へと本研究は発展可能と考える。

研究成果
(19件)

すべてその他

すべて文献書誌 (19件)

[文献書誌] Nishimori H, Yasoshima T, Hata F, Denno R, Yanai Y, et al.: "A Novel Nude Mouse Model of Liver Metastasis and Peritoneal Dissemination from the Same Human Pancreatic Cancer Line"Pancreas. (In press). (2002)
[文献書誌] M.Meguro, T katsuramaki, M Nagayama, H Kimura et al.: "A novel inhibitor of inducible nitric oxide synthase (ONO-1714) prevents critical warm ischemia-reperfusion injury in the pig liver"Transplantation Proceeding. (In press). (2002)
[文献書誌] T.Katsuramaki, K Hirata, T Furuhata et al.: "Preoperative Estimation of Risk in Hepatectomy Using Technetium-99m-Galactosyl Human Serum Albumin Receptor Amount by Nonlinear 3-Compartment Model (01641)"Hepato-Gastroenterology. (In press). (2002)
[文献書誌] T.Katsuramaki, K Hirata, Y Kimura et al.: "Changes in the serum levels of apolipoprotein A-1 as an indicator of protein metabolism after hepatectomy"Wound repair and Regenerution. (In press). (2002)
[文献書誌] Katsura N, Ikai I, Mitaka T, Shiotani T, Matsushita T, et al.: "Long-term culture of primary human hepatocytes with preservation of proliferative capacity and differentiated functions"J Surg Res. (In press). (2002)
[文献書誌] Mizuguchi T, Palm K, Hui T, Aoki T, Mochizuki Y, Mitaka T, et al.: "Effects of bone marrow stromal cells on the structural and functional polarity of primary rat heptocytes"In Vitro Cell Dev Biol. (In press). (2002)
[文献書誌] Takagi C, Ueki K, Ikeuchi H, Kuroiwa T, Kaneko Y, et al.: "Increased Expression of Cell Adhesion kinase β in Human and Rat Crescentic Glomerulonephritis"Am J Kid Dis. 39(1). 174-182 (2002)
[文献書誌] K.Hirata: "Sepsis and cholestasis : basic findings in the sinusoid and bile canaliculus"J Hepatobiliary Pancreat Surg. 8. 20-26 (2001)
[文献書誌] Mitaka T, Sato F, Ikeda S, Sugimoto S, Higaki N, et al.: "Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells"Cell Tissue Res.. 306. 467-471 (2001)
[文献書誌] Mizuguchi T, Hui T, Palm K, Suglyama N, Mitaka T, et al.: "Enhanced proliferation and differentiation of rat hepatocytes cultured with bone marrow stromal cells"J. Cell. Physiol. 189(1). 106-119 (2001)
[文献書誌] Yanai Y, Furuhata T, Kimura Y, Yamaguchi K, Yasoshima T, et al.: "Vascular endothelial growth factor C promotes human gastric carcinoma lymph node metastasis in mice"J. Exp. Clin. Cancer Res.. 20(3). 419-428 (2001)
[文献書誌] Nomura, H., Nishimori H., Yasoshima, T., Hata, F et al.: "A new experimental mouse model of peritoneal dissemination of human gastric cancer cells : analysis of the mechanism of peritoneal dissemination using cDNA macroarrays"Jpn. J. Cancer Res.. 92. 748-754 (2001)
[文献書誌] R.Fukui, F.Hata, T.Yasoshima, T.Honma, F.Nakajima, et al.: "Adenosquamous carcinoma of the colorectum : Report of two cases"J. Exp. Clin. Cancer Res.. 20. 293-296 (2001)
[文献書誌] Nishimori, H.Yasoshima, Y.Dennno, R.Shishido et al.: "Kawaguchi, S. Kamiguchi, K. Isomura, H. Sato, N. and Hirata, K. A New Peritoneal Dissemination Model Established from the Human Pancreatic Cancer Cell Line"Pancreas. 22. 348-356 (2001)
[文献書誌] 桂巻正, 木村康利, 目黒誠, 永山稔, 木村仁, 磯部将人, 他: "肝切除術における肝機能指標としての総分枝鎖アミノ酸/チロシンモル比(BTR)の変動…その有用性と問題点…"外科と代謝・栄養. 35(2). 25-32 (2001)
[文献書誌] N Higaki, T Mitaka, F Sato, K Hirata, Y Mochizuki: "Maintenance of connexin 32 and 26 Expression in primary cultured rat hepatocyteds treated with 3-acetylpyridine"Journal of Gastroenterology and Hepatology. 16. 806-815 (2001)
[文献書誌] Mizuguchi T, Kamohara Y, Hui T, Neuman T, Mitaka T, et al.: "Regulation of c-met expression in rats with acute hepatic failure"J. Surg. Res. 99(2). 385-396 (2001)
[文献書誌] Higaki N, Mitaka T, Sato F, Hirata K, Mochizuki Y.: "Maintenance of connexin 32 and 26 expression in primary cultured rat hepatocytes treated with 3-acetylpyridine"J Gastroenterol Hepatol. 16(7). 806-815 (2001)
[文献書誌] 平田公一, 桂巻正: "クリティカルケア SIRS・ショック・MODS"(株)医学書院. 277 (2001)

2001 年度 実績報告書

体外肝組織再構築モデルを用いた胆汁うっ滞解析と細胞移植による肝不全治療法の研究

研究代表者

平田 公一 札幌医科大学, 医学部, 教授 (50136959)

研究成果

[文献書誌] Nishimori H, Yasoshima T, Hata F, Denno R, Yanai Y, et al.: "A Novel Nude Mouse Model of Liver Metastasis and Peritoneal Dissemination from the Same Human Pancreatic Cancer Line"Pancreas. (In press). (2002)

[文献書誌] M.Meguro, T katsuramaki, M Nagayama, H Kimura et al.: "A novel inhibitor of inducible nitric oxide synthase (ONO-1714) prevents critical warm ischemia-reperfusion injury in the pig liver"Transplantation Proceeding. (In press). (2002)

[文献書誌] T.Katsuramaki, K Hirata, T Furuhata et al.: "Preoperative Estimation of Risk in Hepatectomy Using Technetium-99m-Galactosyl Human Serum Albumin Receptor Amount by Nonlinear 3-Compartment Model (01641)"Hepato-Gastroenterology. (In press). (2002)

[文献書誌] T.Katsuramaki, K Hirata, Y Kimura et al.: "Changes in the serum levels of apolipoprotein A-1 as an indicator of protein metabolism after hepatectomy"Wound repair and Regenerution. (In press). (2002)

[文献書誌] Katsura N, Ikai I, Mitaka T, Shiotani T, Matsushita T, et al.: "Long-term culture of primary human hepatocytes with preservation of proliferative capacity and differentiated functions"J Surg Res. (In press). (2002)

[文献書誌] Mizuguchi T, Palm K, Hui T, Aoki T, Mochizuki Y, Mitaka T, et al.: "Effects of bone marrow stromal cells on the structural and functional polarity of primary rat heptocytes"In Vitro Cell Dev Biol. (In press). (2002)

[文献書誌] Takagi C, Ueki K, Ikeuchi H, Kuroiwa T, Kaneko Y, et al.: "Increased Expression of Cell Adhesion kinase β in Human and Rat Crescentic Glomerulonephritis"Am J Kid Dis. 39(1). 174-182 (2002)

[文献書誌] K.Hirata: "Sepsis and cholestasis : basic findings in the sinusoid and bile canaliculus"J Hepatobiliary Pancreat Surg. 8. 20-26 (2001)

[文献書誌] Mitaka T, Sato F, Ikeda S, Sugimoto S, Higaki N, et al.: "Expression of carbamoylphosphate synthetase I and glutamine synthetase in hepatic organoids reconstructed by rat small hepatocytes and hepatic nonparenchymal cells"Cell Tissue Res.. 306. 467-471 (2001)

[文献書誌] Mizuguchi T, Hui T, Palm K, Suglyama N, Mitaka T, et al.: "Enhanced proliferation and differentiation of rat hepatocytes cultured with bone marrow stromal cells"J. Cell. Physiol. 189(1). 106-119 (2001)

[文献書誌] Yanai Y, Furuhata T, Kimura Y, Yamaguchi K, Yasoshima T, et al.: "Vascular endothelial growth factor C promotes human gastric carcinoma lymph node metastasis in mice"J. Exp. Clin. Cancer Res.. 20(3). 419-428 (2001)

[文献書誌] Nomura, H., Nishimori H., Yasoshima, T., Hata, F et al.: "A new experimental mouse model of peritoneal dissemination of human gastric cancer cells : analysis of the mechanism of peritoneal dissemination using cDNA macroarrays"Jpn. J. Cancer Res.. 92. 748-754 (2001)

[文献書誌] R.Fukui, F.Hata, T.Yasoshima, T.Honma, F.Nakajima, et al.: "Adenosquamous carcinoma of the colorectum : Report of two cases"J. Exp. Clin. Cancer Res.. 20. 293-296 (2001)

[文献書誌] Nishimori, H.Yasoshima, Y.Dennno, R.Shishido et al.: "Kawaguchi, S. Kamiguchi, K. Isomura, H. Sato, N. and Hirata, K. A New Peritoneal Dissemination Model Established from the Human Pancreatic Cancer Cell Line"Pancreas. 22. 348-356 (2001)

[文献書誌] 桂巻正, 木村康利, 目黒誠, 永山稔, 木村仁, 磯部将人, 他: "肝切除術における肝機能指標としての総分枝鎖アミノ酸/チロシンモル比(BTR)の変動…その有用性と問題点…"外科と代謝・栄養. 35(2). 25-32 (2001)

[文献書誌] N Higaki, T Mitaka, F Sato, K Hirata, Y Mochizuki: "Maintenance of connexin 32 and 26 Expression in primary cultured rat hepatocyteds treated with 3-acetylpyridine"Journal of Gastroenterology and Hepatology. 16. 806-815 (2001)

[文献書誌] Mizuguchi T, Kamohara Y, Hui T, Neuman T, Mitaka T, et al.: "Regulation of c-met expression in rats with acute hepatic failure"J. Surg. Res. 99(2). 385-396 (2001)

[文献書誌] Higaki N, Mitaka T, Sato F, Hirata K, Mochizuki Y.: "Maintenance of connexin 32 and 26 expression in primary cultured rat hepatocytes treated with 3-acetylpyridine"J Gastroenterol Hepatol. 16(7). 806-815 (2001)

[文献書誌] 平田公一, 桂巻 正: "クリティカルケア SIRS・ショック・MODS"(株)医学書院. 277 (2001)

2001 年度実績報告書

平田公一札幌医科大学, 医学部, 教授 (50136959)

[文献書誌] 平田公一, 桂巻正: "クリティカルケア SIRS・ショック・MODS"(株)医学書院. 277 (2001)