2002 年度研究成果報告書概要

植物の澱粉生合成関連酵素の機能変換と遺伝子発現制御機構の解明

研究課題

研究課題/領域番号	13660071
研究種目	基盤研究(C)
配分区分	補助金
応募区分	一般
研究分野	応用微生物学・応用生物化学
研究機関	北海道大学
研究代表者	伊藤浩之北海道大学, 大学院・農学研究科, 助教授 (10241366)
研究期間 (年度)	2001 – 2002
キーワード	アロステリック調節 / 形質転換植物 / ADPglucose pyrophosphorylase / 澱粉生合成 / 変異酵素 / プロモーター解析
研究概要	澱粉生合成における唯一のグルコース供与体はADP-グルコースであり,ADP-glucose pyrophosphorylase (AGPase)によって合成される.植物生体内において,AGPaseはアロステリックに調節され,澱粉生合成速度を支配していると考えられている.本研究では,AGPaseのアロステリック特性が澱粉合成量に与える影響を詳細に解析し,最終的な植物の生産性(収量)の増加をめざし,以下に示す成果を得た. (1)葉におけるAGPaseを欠損したシロイヌナズナ(TL46株)に,アロステリック感受性の低下したAGPaseをコードする遺伝子を導入した形質転換植物を作成・解析した. (2)形質転換植物の葉におけるAGPase活性は,TL46株の10倍以上に,また,野生株の1.5倍以上に増加した. (3)形質転換植物葉の澱粉蓄積量は,野生株の1.3倍程度であったが,その代謝回転率は1.8倍に増加した.また,葉の乾物重量ならびに面積も野生株に比べ1.8倍に増加した. (4)形質転換植物の種子の収量は,野生株の1.3倍に増大した. 以上の結果より,AGPaseのアロステリック特性は,澱粉合成速度を調節するだけでなく,植物の生育や収量にも影響し,アロステリック感受性の低下は澱粉合成量,代謝回転ならびに収量の増加をもたらすことが示された.

研究成果

(25件)

すべてその他

すべて文献書誌 (25件)

[文献書誌] K.Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotechnol. Biochem. 65. 1141-1148 (2001)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] T.Mizuno et al.: "Isolation and sequence of a putative α-glucosidase gene from Brevibacterium fuscum var. dextranlyticum strain 0407"J. Appl. Glycosci.. 48. 287-291 (2001)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] V.K.Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161. 467-476 (2001)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] Y.Kakuta et al.: "1-Aminocyclopropane-1-carboxylate synthase of Penicillium citrinum : primary structure and expression in Escherichia coli and Saccharomyces cerevisiae"Biosci. Biotech. Biochem.. 65. 1511-1518 (2001)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] S.Hamada et al.: "Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359. 23-34 (2001)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] K.Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70. 89-109 (2002)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] T.W.Okita et al.: "Increasing rice productivity and yield by manipulation of starch synthesis"Rice Biotechnology : Improving Yield, Stress Tolerance and Grain Quality. (John Wiley and Sons, New York). 18 (2001)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] S.Hamada et al.: "Differential characteristics and subcellular localization of two starch-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L"J. Biol. Chem.. 277. 16538-16546 (2002)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] I.H.Kavakli et al.: "Generation, characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215. 430-439 (2002)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] H.Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stages of development"Carbohydr. Res.. 338. 463-469 (2003)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] 伊藤裕之, 松井博和: "Starch branching enzymeの酵素特性と細胞内局在性"日本農芸化学会誌(ミニレビュー). 77(4). (2003)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] N.Isono et al.: "Molecular cloning of a cDNA encoding granule-bound starch synthase I from kidney bean seeds and expression of Escherichia coli"J. Appl. Glycosci.. (in press). (2003)
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] D.Omoto et al.: "Isolation and characterization of two cDNAs for large and small subunits of ADP-glucose pyrophosphorylase from kidney bean"J. Appl. Glycosci.. (in press).
- 説明
  「研究成果報告書概要(和文)」より
[文献書誌] K. Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotech. Biochem.. 65(5). 1141-1148 (2001)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] T. Mizuno et al.: "Isolation and sequence of a putative α-glucosidase gene from Brevibacterium fuscom var. dextranlyticum strain 0407"J. Appl. Glycosci.. 48(3). 287-291 (2001)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] V.K. Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161(3). 467-476 (2001)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] Y. Kakuta et al.: "1-Aminocyclopropane-1-carboxylate synthase of Penicillium citrinum : Primary structure and expression in Escherichia coil and Saccharomyces cerevisiae"Biosci. Biotech. Biochem.. 65(7). 1511-1518 (2001)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] S. Hamada et al.: "Two starch-branching enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359(1). 23-34 (2001)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] K. Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70(2). 89-109 (2002)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] S. Hamada et al.: "Differential characteristics and subcellular localization of two strach-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L"J. Biol. Chem.. 277(19). 16538-16546 (2002)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] I.H. Kavakli et al.: "Generation , characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215(3). 430-439 (2002)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] H. Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stages of development"Carbohydr. Res.. 338(5). 463-469 (2003)
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] N. Isono et al.: "Molecular cloning of a cDNA encoding granulebound starch synthase I from kidney bean seeds and expression in Escherichia coil"J. Appl. Glycosci.. in press.
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] H. Ito, H. Matsui: "Enzymatic properties and subcellular localization of starch branching enzymes from Phaseolus vulgaris L"Nippon Nogelkagaku Kaishi (Mini Review ; Japanese). in press.
- 説明
  「研究成果報告書概要(欧文)」より
[文献書誌] T.W. Okita et al., eds. By J. A. Goode and D. Chadwick: "Increasing rice productivity and yield by manipulation of starch synthesis, Rice Biotechnology : Improving Yield, Stress Tolerance and Grain Quality"John Wiley and Sons.
- 説明
  「研究成果報告書概要(欧文)」より

2002 年度 研究成果報告書概要

植物の澱粉生合成関連酵素の機能変換と遺伝子発現制御機構の解明

研究代表者

伊藤 浩之 北海道大学, 大学院・農学研究科, 助教授 (10241366)

研究成果

[文献書誌] K.Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotechnol. Biochem. 65. 1141-1148 (2001)

説明

[文献書誌] T.Mizuno et al.: "Isolation and sequence of a putative α-glucosidase gene from Brevibacterium fuscum var. dextranlyticum strain 0407"J. Appl. Glycosci.. 48. 287-291 (2001)

説明

[文献書誌] V.K.Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161. 467-476 (2001)

説明

[文献書誌] Y.Kakuta et al.: "1-Aminocyclopropane-1-carboxylate synthase of Penicillium citrinum : primary structure and expression in Escherichia coli and Saccharomyces cerevisiae"Biosci. Biotech. Biochem.. 65. 1511-1518 (2001)

説明

[文献書誌] S.Hamada et al.: "Two starch-branching-enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359. 23-34 (2001)

説明

[文献書誌] K.Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70. 89-109 (2002)

説明

[文献書誌] T.W.Okita et al.: "Increasing rice productivity and yield by manipulation of starch synthesis"Rice Biotechnology : Improving Yield, Stress Tolerance and Grain Quality. (John Wiley and Sons, New York). 18 (2001)

説明

[文献書誌] S.Hamada et al.: "Differential characteristics and subcellular localization of two starch-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L"J. Biol. Chem.. 277. 16538-16546 (2002)

説明

[文献書誌] I.H.Kavakli et al.: "Generation, characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215. 430-439 (2002)

説明

[文献書誌] H.Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stages of development"Carbohydr. Res.. 338. 463-469 (2003)

説明

[文献書誌] 伊藤裕之, 松井博和: "Starch branching enzymeの酵素特性と細胞内局在性"日本農芸化学会誌(ミニレビュー). 77(4). (2003)

説明

[文献書誌] N.Isono et al.: "Molecular cloning of a cDNA encoding granule-bound starch synthase I from kidney bean seeds and expression of Escherichia coli"J. Appl. Glycosci.. (in press). (2003)

説明

[文献書誌] D.Omoto et al.: "Isolation and characterization of two cDNAs for large and small subunits of ADP-glucose pyrophosphorylase from kidney bean"J. Appl. Glycosci.. (in press).

説明

[文献書誌] K. Nozaki et al.: "Major isoforms of starch branching enzymes in premature seeds of kidney bean (Phaseolus vulgaris L.)"Biosci. Biotech. Biochem.. 65(5). 1141-1148 (2001)

説明

[文献書誌] T. Mizuno et al.: "Isolation and sequence of a putative α-glucosidase gene from Brevibacterium fuscom var. dextranlyticum strain 0407"J. Appl. Glycosci.. 48(3). 287-291 (2001)

説明

[文献書誌] V.K. Sikka et al.: "Subcellular compartmentation and allosteric regulation of the rice endosperm ADPglucose pyrophosphorylase"Plant Sci.. 161(3). 467-476 (2001)

説明

[文献書誌] Y. Kakuta et al.: "1-Aminocyclopropane-1-carboxylate synthase of Penicillium citrinum : Primary structure and expression in Escherichia coil and Saccharomyces cerevisiae"Biosci. Biotech. Biochem.. 65(7). 1511-1518 (2001)

説明

[文献書誌] S. Hamada et al.: "Two starch-branching enzyme isoforms occur in different fractions of developing seeds of kidney bean"Biochem. J.. 359(1). 23-34 (2001)

説明

[文献書誌] K. Nozaki et al.: "Heterogeneity of the morphology of growing starch granules at the developing seeds of kidney bean (Phaseolus vulgaris L.)"J. Grad. Sch. Agr. Hokkaido Univ.. 70(2). 89-109 (2002)

説明

[文献書誌] S. Hamada et al.: "Differential characteristics and subcellular localization of two strach-branching enzyme isoforms encoded by a single gene in Phaseolus vulgaris L"J. Biol. Chem.. 277(19). 16538-16546 (2002)

説明

[文献書誌] I.H. Kavakli et al.: "Generation , characterization, and heterologous expression of wild-type and up-regulated forms of Arabidopsis thaliana leaf ADP-glucose pyrophosphorylase"Planta. 215(3). 430-439 (2002)

説明

[文献書誌] H. Yoshida et al.: "Structure and physicochemical properties of starches from kidney bean seeds at immature, premature and mature stages of development"Carbohydr. Res.. 338(5). 463-469 (2003)

説明

[文献書誌] N. Isono et al.: "Molecular cloning of a cDNA encoding granulebound starch synthase I from kidney bean seeds and expression in Escherichia coil"J. Appl. Glycosci.. in press.

説明

[文献書誌] H. Ito, H. Matsui: "Enzymatic properties and subcellular localization of starch branching enzymes from Phaseolus vulgaris L"Nippon Nogelkagaku Kaishi (Mini Review ; Japanese). in press.

説明

[文献書誌] T.W. Okita et al., eds. By J. A. Goode and D. Chadwick: "Increasing rice productivity and yield by manipulation of starch synthesis, Rice Biotechnology : Improving Yield, Stress Tolerance and Grain Quality"John Wiley and Sons.

説明

2002 年度研究成果報告書概要

伊藤浩之北海道大学, 大学院・農学研究科, 助教授 (10241366)