| 研究実績の概要 |
For the FY2014, we have completed the main bioinformatics analyses required for this project, using the collected transcriptome data from multiple Drosophila cell types at different stages of development using the CAGE experimental protocol. We decided to focus on: 1) Class IV da neuron development time course, and 2) specification of Class IV vs. the Class I-III cell types. We performed the following analyses:
1. Characterization of the expressed promoters based on their sequence composition and shapes (whether they are sharply peaked or broadly spread), and how they are differentially utilized.
2. Collecting and clustering of Drosophila-specific transcription factor binding site motifs and predicting their positions on the Drosophila genome. Due to the lack of available Drosophila-specific binding motifs, we collected and merged motifs based on their similarity.
3. Regulatory analysis of the differentially expressed promoters based on the collected motif set and available ChIP-seq and ChIP-chip data. We have come up with a list of candidate regulatory factors and are in the process of validating them experimentally.
4. Analysis of the repeat transcriptome for the analyzed samples. We found that certain repeats are differentially expressed according to developmental stages or cell types, and we are trying to determine what regulatory controls are behind these differences.
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