2001 年度実績報告書

哺乳類卵胞の選択的死滅制御機構とその人為支配による潜在的卵巣卵の利用

研究課題

研究課題/領域番号	13GS0008
研究種目	創成的基礎研究費
研究機関	京都大学
研究代表者	眞鍋昇京都大学, 農学研究科, 助教授 (80243070)
研究分担者	杉本実紀京都大学, 農学研究科, 助手 (20243074) 宮野隆神戸大学, 農学部, 助教授 (80200195) 酒巻和弘京都大学, 生命科学研究科, 助教授 (20271017) 中山瑞穂日本学術振興会, 特別研究員(PD)
キーワード	哺乳類 / 卵巣 / 卵胞 / 選択的死滅 / アポトーシス / 細胞死受容体 / カスパーゼ / 異種移植
研究概要	サケのように一生に一度だけ全卵子を排卵する動物と異なり、哺乳類は性周期毎に一定数を成熟・排卵することを繰り返す。哺乳類の片側卵巣には、胎児期に減数分裂前期後半のディプロテン期で休止した卵母細胞が5〜50万個含まれている。出産後性成熟期に達すると、性周期毎に卵母細胞はそれを取り囲む卵胞の発達とともに発育する。この時卵巣内では最終的に排卵にいたる卵子の100倍以上の卵胞が発達する。この卵胞発達とそれに引き続いて起こる卵母細胞の成熟の過程で、99%以上の卵母細胞は選択的に死滅し、ごく一部の選抜された卵子のみが排卵にいたる。この卵胞の選択的死滅は、より優秀で強靭な子孫を残す戦略として重要であるが、この選択的死滅を制御している分子機構は未だ解明されていない。本研究は、未解明な哺乳類卵巣において発育・成熟中の卵母細胞を含む卵胞の選択的死滅を制御している分子機構を解明し、この知見をもとに細胞死を制御している遺伝子の発現をin vivo制御することで卵胞の発達を人為支配し、卵巣内に潜在する卵母細胞の有効利用を実現することが目的である。今年度は、新規な細胞死受容体を認識するユニークなモノクロナル抗体を作製し、それを用いて、新たに囮(デコイ)受容体を発見できた。また、卵巣で発現していることが知られている細胞死受容体であるFasの細胞内シグナル伝達系を担うカスパーゼをノックアウトしたマウスを用いて、卵巣におけるアポトーシスシグナル伝達系の分子制御機構を解明した。一方でウシ、ブタなどの大型哺乳類の未成熟な卵胞を免疫不全マウスの腎に移植(異種移植)し、発育・成熟する技術システムを確立した。

研究成果
(34件)

すべてその他

すべて文献書誌 (34件)

[文献書誌] Satoko Wada, Noboru Manabe et al.: "TRAIL-decoy receptor-1 disappears in granulosa cells of atretic follicles in porcine ovaries"Journal of Reproduction and Devevelopment. 48・2. 167-173 (2002)
[文献書誌] Satoko Wada, Noboru Manabe et al.: "TRADD is involved in apoptosis induction in granulosa cells during atresia in pig ovaries"Journal of Reproduction and Devevelopment. 48・2. 175-181 (2002)
[文献書誌] Hitoshi Okamura, Noboru Manabe et al.: "Protein tyrosine kinase expression in the porcine ovary"Molecular Human Reproduction. 7・6. 723-729 (2001)
[文献書誌] Eiichi Hondo, Noboru Manabe et al.: "Molecular cloning and expression of suppressor of potassium transport defect 3 (SKD3) in rat testis"Journal of Reproduction and Devevelopment. 47・2. 173-180 (2001)
[文献書誌] Minako Kiso, Noboru Manabe et al.: "Abnormal accumulation of luteal bodies in ovaries of the senescence accelerated mouse (SAM)"Journal of Reproduction and Devevelopment. 47・2. 153-164 (2001)
[文献書誌] Mizuho Nakayama, Noboru Manabe et al.: "Changes in localization of type I, III and IV collagens and their mRNA expression during follicular atresia in bovine and porcine ovaries"Journal of Reproduction and Devevelopment. 47・1. 27-31 (2001)
[文献書誌] Noboru Manabe et al.: "A novel in vivo NMR technique to assess halocarbon induced hepatic failure in mice"Water Science and Technology. 17・1. 32-38 (2001)
[文献書誌] Noboru Manabe et al.: "In vivo noninvasive nuclear magnetic resonance (NMR) technique for assessment of teratogenicity induced by chemicals"Chemical Engineering. 47・1. 110-114 (2002)
[文献書誌] Miki Sugimoto, Noboru Manabe et al.: "Update on cryopreservation of mammalian ovaries"Obstetrics and Gynecology. 68・2. 207-212 (2001)
[文献書誌] Noboru Manabe et al.: "A novel cell death receptor and a decoy receptor on follicular granulose cells of porcine ovaries"Japanese Journal of Embryo Transfer. 23・1. 52-60 (2001)
[文献書誌] Naoko Inoue, Noboru Manabe et al.: "Elongation of cell cycle of early stage tetraploid murine embryos induced by cytochalasin B"Japanese Journal of Embryo Transfer. 23・1. 44-51 (2001)
[文献書誌] Noboru Manabe et al.: "Physiological roles of apoptosis in granulosa cells of atretic follicles in porcine ovaries"Japanese Journal of Embryo Transfer. 23・1. 1-10 (2001)
[文献書誌] Noboru Manabe et al.: "Monoclonal antibodies recognize a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles"Journal of Mammalian Ova Research. 18・1. 1-13 (2001)
[文献書誌] Noboru Manabe et al.: "Immunohistochemical Quantification of fast-myosin in upper hind limp muscles of the pig"Animal Science Journal. 72・2. 154-163 (2001)
[文献書誌] Hideki Tonomura, Nobor Manabe et al.: "Detection of rat model useful for genetic analysis of intramascular fat accumulation"Animal Genetic Journal. 2・1. 3-10 (2001)
[文献書誌] Brussow K-P, Noboru Manabe et al.: "Biotechnological aspects of embryo transfer in swine"Hungarian Animal Breeding and Nutrition. 50・4. 481-493 (2001)
[文献書誌] Noboru Manabe et al.: "Changes in quantitative profile of extracellular matrix components in the kidneys of rats with adriamycin-induced nephropathy"Journal of Veterinary Medical Science. 61・1. 125-133 (2001)
[文献書誌] Misuzu Yamaguchi, Noboru Manabe et al.: "Localization of proliferative and apoptptic cells in the kidneys of ICR-derives glomerulonephritis (ICGN) mice"Journal of Veterinary Medical Science. 62・6. 781-787 (2001)
[文献書誌] Kozue Uchio-Yamada, Noboru Manabe et al.: "Localization of extracellular matrix receptors in ICGN mice, a strain of mice with hereditary nephrotic syndrome"Journal of Veterinary Medical Science. 62・11. 1171-1178 (2002)
[文献書誌] Malki Morita, Noboru Manabe et al.: "Changes in morphology of mouse oviduct ampulla during early pregnancy"Biology of Reproduction. 64・2. 325 (2001)
[文献書誌] Noboru Manabe et al.: "Teratosis induced by retinoites to apoptotic cell death in mouse embryos"Miyamoto H, Manabe N (eds), Reproductive biotechnology update and its related physiology Hokuto Publishing Co., (Kyoto, Japan). 424 (2001)
[文献書誌] Takako Tanaka, Noboru Manabet al.: "Effects of endocrine disruptersrts on the expression of nuclear receptors in murine embryos"Miyamoto H, Manabe N (eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Miki Sugimoto, Noboru Manabe et al.: "Uilization of NMR microscopy in developmental toxicology : assessment of mouse embryo malformations induced by maternal administration of retinoic acid"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Noboru Manabe et al.: "Quantitative estimation of physiological properties by histochemical techniques"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Noboru Manabe et al.: "In vivo 31P-nuclear magnetic resonance technique for estibation of liver energy metabolism in mice"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Miki SUGIMOTO, Noboru Manabe et al.: "Histological and biochemical profiles of granulosa cell apoptosis in follicular atresia of porcine ovaries"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Noboru Manabe et al.: "Cell death receptor dependent regulation of granulosa cell apoptosis in porcine ovarian follicle atresia"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Mizuho Nakayama, Noboru Manabe et al.: "Histochemical studies for comparison of regulatory mechanisms of follicular development in mammmalian ovaries"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Namiko Kiso, Noboru Manabe et al.: "Abnormal accumulation of luteal bodies in ovaries of the senescence accelerated mouse(SAM)"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Maki Morita, Noboru Manabe et al.: "Functions of mammalian oviducts and their relevance to morphology"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Kozue Yamada-Uchio, Noboru Manabe et al.: "Abnormalities of extracellular matrix metabolisms in the kidney of ICGN mice, a strain of mice with hereditary nephrotic syndrome"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Misuzu Yamaguchi, Noboru Manabe et al.: "Renal anemia in ICR-derived glomerulonephritis (ICGN) mice"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] Miki Sugimoto, Noboru Manabe et al.: "Cryopreservation of mammalian ovarian tissue"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)
[文献書誌] 眞鍋昇: "雌性生殖器官"獣医組織学第2版,日本獣医解剖学会編集(学窓社.東京.). 448 (2001)

2001 年度 実績報告書

哺乳類卵胞の選択的死滅制御機構とその人為支配による潜在的卵巣卵の利用

研究代表者

眞鍋 昇 京都大学, 農学研究科, 助教授 (80243070)

研究成果

[文献書誌] Satoko Wada, Noboru Manabe et al.: "TRAIL-decoy receptor-1 disappears in granulosa cells of atretic follicles in porcine ovaries"Journal of Reproduction and Devevelopment. 48・2. 167-173 (2002)

[文献書誌] Satoko Wada, Noboru Manabe et al.: "TRADD is involved in apoptosis induction in granulosa cells during atresia in pig ovaries"Journal of Reproduction and Devevelopment. 48・2. 175-181 (2002)

[文献書誌] Hitoshi Okamura, Noboru Manabe et al.: "Protein tyrosine kinase expression in the porcine ovary"Molecular Human Reproduction. 7・6. 723-729 (2001)

[文献書誌] Eiichi Hondo, Noboru Manabe et al.: "Molecular cloning and expression of suppressor of potassium transport defect 3 (SKD3) in rat testis"Journal of Reproduction and Devevelopment. 47・2. 173-180 (2001)

[文献書誌] Minako Kiso, Noboru Manabe et al.: "Abnormal accumulation of luteal bodies in ovaries of the senescence accelerated mouse (SAM)"Journal of Reproduction and Devevelopment. 47・2. 153-164 (2001)

[文献書誌] Mizuho Nakayama, Noboru Manabe et al.: "Changes in localization of type I, III and IV collagens and their mRNA expression during follicular atresia in bovine and porcine ovaries"Journal of Reproduction and Devevelopment. 47・1. 27-31 (2001)

[文献書誌] Noboru Manabe et al.: "A novel in vivo NMR technique to assess halocarbon induced hepatic failure in mice"Water Science and Technology. 17・1. 32-38 (2001)

[文献書誌] Noboru Manabe et al.: "In vivo noninvasive nuclear magnetic resonance (NMR) technique for assessment of teratogenicity induced by chemicals"Chemical Engineering. 47・1. 110-114 (2002)

[文献書誌] Miki Sugimoto, Noboru Manabe et al.: "Update on cryopreservation of mammalian ovaries"Obstetrics and Gynecology. 68・2. 207-212 (2001)

[文献書誌] Noboru Manabe et al.: "A novel cell death receptor and a decoy receptor on follicular granulose cells of porcine ovaries"Japanese Journal of Embryo Transfer. 23・1. 52-60 (2001)

[文献書誌] Naoko Inoue, Noboru Manabe et al.: "Elongation of cell cycle of early stage tetraploid murine embryos induced by cytochalasin B"Japanese Journal of Embryo Transfer. 23・1. 44-51 (2001)

[文献書誌] Noboru Manabe et al.: "Physiological roles of apoptosis in granulosa cells of atretic follicles in porcine ovaries"Japanese Journal of Embryo Transfer. 23・1. 1-10 (2001)

[文献書誌] Noboru Manabe et al.: "Monoclonal antibodies recognize a novel cell death receptor and a decoy receptor on granulosa cells of porcine ovarian follicles"Journal of Mammalian Ova Research. 18・1. 1-13 (2001)

[文献書誌] Noboru Manabe et al.: "Immunohistochemical Quantification of fast-myosin in upper hind limp muscles of the pig"Animal Science Journal. 72・2. 154-163 (2001)

[文献書誌] Hideki Tonomura, Nobor Manabe et al.: "Detection of rat model useful for genetic analysis of intramascular fat accumulation"Animal Genetic Journal. 2・1. 3-10 (2001)

[文献書誌] Brussow K-P, Noboru Manabe et al.: "Biotechnological aspects of embryo transfer in swine"Hungarian Animal Breeding and Nutrition. 50・4. 481-493 (2001)

[文献書誌] Noboru Manabe et al.: "Changes in quantitative profile of extracellular matrix components in the kidneys of rats with adriamycin-induced nephropathy"Journal of Veterinary Medical Science. 61・1. 125-133 (2001)

[文献書誌] Misuzu Yamaguchi, Noboru Manabe et al.: "Localization of proliferative and apoptptic cells in the kidneys of ICR-derives glomerulonephritis (ICGN) mice"Journal of Veterinary Medical Science. 62・6. 781-787 (2001)

[文献書誌] Kozue Uchio-Yamada, Noboru Manabe et al.: "Localization of extracellular matrix receptors in ICGN mice, a strain of mice with hereditary nephrotic syndrome"Journal of Veterinary Medical Science. 62・11. 1171-1178 (2002)

[文献書誌] Malki Morita, Noboru Manabe et al.: "Changes in morphology of mouse oviduct ampulla during early pregnancy"Biology of Reproduction. 64・2. 325 (2001)

[文献書誌] Noboru Manabe et al.: "Teratosis induced by retinoites to apoptotic cell death in mouse embryos"Miyamoto H, Manabe N (eds), Reproductive biotechnology update and its related physiology Hokuto Publishing Co., (Kyoto, Japan). 424 (2001)

[文献書誌] Noboru Manabe et al.: "Quantitative estimation of physiological properties by histochemical techniques"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)

[文献書誌] Maki Morita, Noboru Manabe et al.: "Functions of mammalian oviducts and their relevance to morphology"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)

[文献書誌] Misuzu Yamaguchi, Noboru Manabe et al.: "Renal anemia in ICR-derived glomerulonephritis (ICGN) mice"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)

[文献書誌] Miki Sugimoto, Noboru Manabe et al.: "Cryopreservation of mammalian ovarian tissue"Miyamoto H, Manabe N(eds), Reproductive Biotechnology : Reproductive biotechnology update and its related physiology(Hokuto Publishing Co., (Kyoto, Japan)). 424 (2001)

[文献書誌] 眞鍋昇: "雌性生殖器官"獣医組織学 第2版,日本獣医解剖学会編集(学窓社.東京.). 448 (2001)

2001 年度実績報告書

眞鍋昇京都大学, 農学研究科, 助教授 (80243070)

[文献書誌] 眞鍋昇: "雌性生殖器官"獣医組織学第2版,日本獣医解剖学会編集(学窓社.東京.). 448 (2001)