2002 年度実績報告書

太陽光下の水環境系における光機能界面での活性酸素の生成と計測

研究課題

研究課題/領域番号	14050038
研究機関	東京工業大学
研究代表者	大坂武男東京工業大学, 大学院・総合理工学研究科, 教授 (80152099)
研究分担者	岡島武義東京工業大学, 大学院・総合理工学研究科, 助手 (70194347)
キーワード	光機能界面 / 活性酸素 / 過酸化水素 / 酸素 / スーパーオキシドイオン / 電気化学測定 / 自己組織化単分子膜 / 光触媒
研究概要	本研究は,自然界の太陽光下水環境系での光機能界面モデルとして,光照射下の空気/水溶液界相を設定し,この界相での活性酸素生成の検証及びそのための新たな検出法の開発を目的として行われ、次の成果を得た。 1.水環境系での活性酸素の測定には,その寿命や生成環境を考えるとオンサイト測定法が必要であり,そのための新たな電気化学的検出法を提案した。 (1)H_2O_2分解触媒の存在下ビオロゲンの自己組織化単分子膜(SAM)修飾電極(直径:1.6mm)を用いることにより、0.1μM〜10mMのH_2O_2を検出できることを明らかにした。 (2)直径15μmの金ディスクを300μmの間隔で配列させた"金マイクロデスク配列電極"を用いることにより0.1μM〜100μMのH_2O_2の検出が可能であることを明らかにした。 (3)金電極に構築したシステインSAM上にスーパーオキシドジスムターゼ(SOD)が安定に固定され,その直接電極反応が著しく促進されること,そしてこのSOD修飾電極が"第3世代のスーパーオキシドイオンバイオセンサー"として機能することをはじめて明らかにした。 2.酸素/水溶液(飽和の腐植酸を含む)界面に光照射した場合(Xeランプ,98 mW/cm^2),1.9n mol/hのH_2O_2の生成を確認した。また,光を照射しない場合及び窒素雰囲気下ではH_2O_2は生成しないことがわかった。

研究成果
(34件)

すべてその他

すべて文献書誌 (34件)

[文献書誌] T.Okajima: "Electrogenerated Chemiluminescence of Lucigenin Enhanced by the Modification of Electrodes with Self-Assembled Monlayers and of Solutions with Surfactants"J.Electroanal.Chem.. 524. 181-187 (2002)
[文献書誌] D.Zhang: "Electrochemical Reduction of Dioxygen on Mercury Electrode in Alkaline Aqueous Media"Jpn.J.Deuterium Sci.. 11・1. 1-8 (2002)
[文献書誌] C.R.Raj: "Voltammetric Measurements of pH Using a Mixed Self-assembly of Macrocyclic Nickel (II) Complex and Diimine on Gold Electrode"Electrochem.Commun.. 4. 330-335 (2002)
[文献書誌] C.R.Raj: "Analytical Applications of Functionalized Self-Assembled Monolayers on Gold Electrodes"Electroanalysis. 14・10. 679-683 (2002)
[文献書誌] T.Okajima: "Chemiluminescence of Indole and its Derivatives Induce by Electrogenerated Superoxide Ion in Acetonitrile Solutions"Electrochim.Acta. 47. 1561-1565 (2002)
[文献書誌] T.Okajima: "Chemiluminescence of 3-Methylindole Based on Electrogenerated Superoxide Ion"J.Electroanal.Chem.. 523. 34-39 (2002)
[文献書誌] M.S.El-Deab: "Hydrodynamic Voltammetric Studies of the Oxygen Reduction at Gold Nanoparticles-Electrodeposited Gold Electrodes"Electrochim.Acta. 47. 4255-4261 (2002)
[文献書誌] C.R.Raj: "Square Wave Voltammetric Sensing of Uric Acid Using the Self-Assembly of Mercaptobenzimidazole"Analyst. 127. 1155-1158 (2002)
[文献書誌] M.S.El-Deab: "An Extraordinary Electrocatalytic Reduction of Oxygen on Gold Nanoparticles-Electrodeposited Gold Electrodes"Electrochem.Commun.. 4. 288-292 (2002)
[文献書誌] T.Ohsaka: "A Superoxide Dismutase-Modified Electrode That Detects Superoxide Ion"Chem.Commun.. 990-991 (2002)
[文献書誌] L.Mao: "Electrochemical Characterization of Catalytic Activities of Manganese Oxides to Oxygen Reduction in Alkaline Aqueous Solution"J.Electrochem.Soc.. 149. A504-A507 (2002)
[文献書誌] Y.Tian: "A Facilitated Electron Transfer of Copper-Zinc Superoxide Dismutase (SOD) Based on a Cysteine-Bridged SOD Electrode"Biochem.Biophys.Acta. 1569. 151-158 (2002)
[文献書誌] Y.Tian: "Superoxide Dismutase-Based Third-Generation Biosensors for Superoxide Anion"Anal.Chem.. 74. 2428-2434 (2002)
[文献書誌] M.S.Saha: "Interferometric Measurement of the Current Oscillation in the Redox reaction of the O_2/O_2-couple at a HMDE"J.Phys.Chem.B. 106. 4457-4461 (2002)
[文献書誌] 大木成政: "アルカリ性水溶液中におけるポリアニリンスルホン酸修飾電極を用いたスーパーオキシドイオンの電解生成"Electrochemistry. 70・1. 100-110 (2002)
[文献書誌] Y.Tian: "A SOD-Based Amperometric Biosensor for Superoxide Ion"J.Korean Electrochem.Soc.. 5・4. 212-215 (2002)
[文献書誌] T.Oritani: "Simultaneous Electroanalysis of Nitric Oxide and Nitrite"J.Korean Electrochem.Soc.. 5・4. 209-211 (2002)
[文献書誌] K.Arihara: "In situ Monitoring of the Mercury Electrode Surface in HClO-4 Solutions by Means of Infrared Reflection Absorption Spectroscopy"J.Electroanal.Chem.. 518. 139-143 (2002)
[文献書誌] K.Arihara: "Characteristic Cyclic Voltammograms of Alkyl Viologens at Single Crystal Gold Electrodes"Phys.Chem.Chem.Phys.. 4. 1002-1005 (2002)
[文献書誌] P.R.Roy: "Simultaneous electroanalysis of dopamine and ascorbic acid using poly (N, N-dimethylaniline)-modified electrodes"Bioelectrochemistry. 57. 1-9 (2002)
[文献書誌] T.Okajima: "Chemiluminescence of Lucigenin by Electrogenerated Superoxide Ions in Aqueous Solutions"Luminescence. 18. 49-57 (2003)
[文献書誌] C.R.Raj: "Voltammetric Detection of Uric Acid in the Presence of Ascorbic Acid at a Gold Electrode Modified with Self-Assembled Monolayer of Heteroaromatic Thiol"J.Electroanal.Chem.. 540. 69-77 (2003)
[文献書誌] L.Mao: "Mechanistic Study of the Reduction of Oxygen in Air Electrode with Manganese Oxides as Electrocatalysts"Electrochim.Acta. 48・8. 1015-1021 (2003)
[文献書誌] C.R.Raj: "Gold Nano Particle Arrays for the Voltammetric Sensing of Dopamine"J.Electroanal.Chem.. 543・2. 127-133 (2003)
[文献書誌] M.S.El-Deab: "Electrochemical Reduction of Oxygen on Gold Nanoparticles-Electrodeposited Glassy Carbon Electrodes"J.Electrochem.Soc.. (in press). (2003)
[文献書誌] M.S.El-Deab: "Quasi-Reversible Two-Electron Reduction of Oxygen at Gold Electrodes Modifide with a Self-Assembled Submonolayer of Cystein"Electrochem.Commun.. 5・3. 214-219 (2003)
[文献書誌] D.Zhang: "Hydrodynamic Chronocoulometric Estimation of Diffusion Coefficients and Saturated Concentrations of Dioxygen in KOH Solutions"Indian J.Chem.(A). (in press). (2003)
[文献書誌] F.Matsumoto: "Electrogeneration of Superoxide Ion and Its Mechanism at Thiol-Modified Au Electrodes in Alkaline Aqueous Solution"Electrochemistry. (in press). (2003)
[文献書誌] M.I.Awad: "Kinetic Studies of the Oxidation of Iodide by Peroxyacetic Acid"Inorg.Chim.Acta. 344. 253-256 (2003)
[文献書誌] M.I.Awad: "Potentiometric Analysis of Peroxyacetic Acid in the Presence of a Large Excess of Hydrogen Peroxide"J.Electroanal.Chem.. (in press). (2003)
[文献書誌] M.I.Awad: "A Highly Selective Simultaneous Potentiometric Determination of Peroxyacetic Acid and Hydrogen Peroxyde"Anal.Chem.. (in press). (2003)
[文献書誌] 岡島武義: "サイクリックボルタンメトリーによる電極反応の可逆性及び電極反応機構の診断"Electrochemistry. 70・8. 649-655 (2002)
[文献書誌] 大坂武男(分担): "電気化学測定マニュアル(基礎編)"電気化学会編,丸善(株). 151 (2002)
[文献書誌] T.Ohsaka(分担): "Metal Complexes and Metals in Macromolecules"Wiley-VCH(in press). (2003)

2002 年度 実績報告書

太陽光下の水環境系における光機能界面での活性酸素の生成と計測

研究代表者

大坂 武男 東京工業大学, 大学院・総合理工学研究科, 教授 (80152099)

研究成果

[文献書誌] T.Okajima: "Electrogenerated Chemiluminescence of Lucigenin Enhanced by the Modification of Electrodes with Self-Assembled Monlayers and of Solutions with Surfactants"J.Electroanal.Chem.. 524. 181-187 (2002)

[文献書誌] D.Zhang: "Electrochemical Reduction of Dioxygen on Mercury Electrode in Alkaline Aqueous Media"Jpn.J.Deuterium Sci.. 11・1. 1-8 (2002)

[文献書誌] C.R.Raj: "Voltammetric Measurements of pH Using a Mixed Self-assembly of Macrocyclic Nickel (II) Complex and Diimine on Gold Electrode"Electrochem.Commun.. 4. 330-335 (2002)

[文献書誌] C.R.Raj: "Analytical Applications of Functionalized Self-Assembled Monolayers on Gold Electrodes"Electroanalysis. 14・10. 679-683 (2002)

[文献書誌] T.Okajima: "Chemiluminescence of Indole and its Derivatives Induce by Electrogenerated Superoxide Ion in Acetonitrile Solutions"Electrochim.Acta. 47. 1561-1565 (2002)

[文献書誌] T.Okajima: "Chemiluminescence of 3-Methylindole Based on Electrogenerated Superoxide Ion"J.Electroanal.Chem.. 523. 34-39 (2002)

[文献書誌] M.S.El-Deab: "Hydrodynamic Voltammetric Studies of the Oxygen Reduction at Gold Nanoparticles-Electrodeposited Gold Electrodes"Electrochim.Acta. 47. 4255-4261 (2002)

[文献書誌] C.R.Raj: "Square Wave Voltammetric Sensing of Uric Acid Using the Self-Assembly of Mercaptobenzimidazole"Analyst. 127. 1155-1158 (2002)

[文献書誌] M.S.El-Deab: "An Extraordinary Electrocatalytic Reduction of Oxygen on Gold Nanoparticles-Electrodeposited Gold Electrodes"Electrochem.Commun.. 4. 288-292 (2002)

[文献書誌] T.Ohsaka: "A Superoxide Dismutase-Modified Electrode That Detects Superoxide Ion"Chem.Commun.. 990-991 (2002)

[文献書誌] L.Mao: "Electrochemical Characterization of Catalytic Activities of Manganese Oxides to Oxygen Reduction in Alkaline Aqueous Solution"J.Electrochem.Soc.. 149. A504-A507 (2002)

[文献書誌] Y.Tian: "A Facilitated Electron Transfer of Copper-Zinc Superoxide Dismutase (SOD) Based on a Cysteine-Bridged SOD Electrode"Biochem.Biophys.Acta. 1569. 151-158 (2002)

[文献書誌] Y.Tian: "Superoxide Dismutase-Based Third-Generation Biosensors for Superoxide Anion"Anal.Chem.. 74. 2428-2434 (2002)

[文献書誌] M.S.Saha: "Interferometric Measurement of the Current Oscillation in the Redox reaction of the O_2/O_2-couple at a HMDE"J.Phys.Chem.B. 106. 4457-4461 (2002)

[文献書誌] 大木成政: "アルカリ性水溶液中におけるポリアニリンスルホン酸修飾電極を用いたスーパーオキシドイオンの電解生成"Electrochemistry. 70・1. 100-110 (2002)

[文献書誌] Y.Tian: "A SOD-Based Amperometric Biosensor for Superoxide Ion"J.Korean Electrochem.Soc.. 5・4. 212-215 (2002)

[文献書誌] T.Oritani: "Simultaneous Electroanalysis of Nitric Oxide and Nitrite"J.Korean Electrochem.Soc.. 5・4. 209-211 (2002)

[文献書誌] K.Arihara: "In situ Monitoring of the Mercury Electrode Surface in HClO-4 Solutions by Means of Infrared Reflection Absorption Spectroscopy"J.Electroanal.Chem.. 518. 139-143 (2002)

[文献書誌] K.Arihara: "Characteristic Cyclic Voltammograms of Alkyl Viologens at Single Crystal Gold Electrodes"Phys.Chem.Chem.Phys.. 4. 1002-1005 (2002)

[文献書誌] P.R.Roy: "Simultaneous electroanalysis of dopamine and ascorbic acid using poly (N, N-dimethylaniline)-modified electrodes"Bioelectrochemistry. 57. 1-9 (2002)

[文献書誌] T.Okajima: "Chemiluminescence of Lucigenin by Electrogenerated Superoxide Ions in Aqueous Solutions"Luminescence. 18. 49-57 (2003)

[文献書誌] C.R.Raj: "Voltammetric Detection of Uric Acid in the Presence of Ascorbic Acid at a Gold Electrode Modified with Self-Assembled Monolayer of Heteroaromatic Thiol"J.Electroanal.Chem.. 540. 69-77 (2003)

[文献書誌] L.Mao: "Mechanistic Study of the Reduction of Oxygen in Air Electrode with Manganese Oxides as Electrocatalysts"Electrochim.Acta. 48・8. 1015-1021 (2003)

[文献書誌] C.R.Raj: "Gold Nano Particle Arrays for the Voltammetric Sensing of Dopamine"J.Electroanal.Chem.. 543・2. 127-133 (2003)

[文献書誌] M.S.El-Deab: "Electrochemical Reduction of Oxygen on Gold Nanoparticles-Electrodeposited Glassy Carbon Electrodes"J.Electrochem.Soc.. (in press). (2003)

[文献書誌] M.S.El-Deab: "Quasi-Reversible Two-Electron Reduction of Oxygen at Gold Electrodes Modifide with a Self-Assembled Submonolayer of Cystein"Electrochem.Commun.. 5・3. 214-219 (2003)

[文献書誌] D.Zhang: "Hydrodynamic Chronocoulometric Estimation of Diffusion Coefficients and Saturated Concentrations of Dioxygen in KOH Solutions"Indian J.Chem.(A). (in press). (2003)

[文献書誌] F.Matsumoto: "Electrogeneration of Superoxide Ion and Its Mechanism at Thiol-Modified Au Electrodes in Alkaline Aqueous Solution"Electrochemistry. (in press). (2003)

[文献書誌] M.I.Awad: "Kinetic Studies of the Oxidation of Iodide by Peroxyacetic Acid"Inorg.Chim.Acta. 344. 253-256 (2003)

[文献書誌] M.I.Awad: "Potentiometric Analysis of Peroxyacetic Acid in the Presence of a Large Excess of Hydrogen Peroxide"J.Electroanal.Chem.. (in press). (2003)

[文献書誌] M.I.Awad: "A Highly Selective Simultaneous Potentiometric Determination of Peroxyacetic Acid and Hydrogen Peroxyde"Anal.Chem.. (in press). (2003)

[文献書誌] 岡島武義: "サイクリックボルタンメトリーによる電極反応の可逆性及び電極反応機構の診断"Electrochemistry. 70・8. 649-655 (2002)

[文献書誌] 大坂武男(分担): "電気化学測定マニュアル(基礎編)"電気化学会編,丸善(株). 151 (2002)

[文献書誌] T.Ohsaka(分担): "Metal Complexes and Metals in Macromolecules"Wiley-VCH(in press). (2003)

2002 年度実績報告書

大坂武男東京工業大学, 大学院・総合理工学研究科, 教授 (80152099)