2003 年度実績報告書

塩類細胞の分子解剖と分化誘導機構

研究課題

研究課題/領域番号	14104002
研究機関	東京工業大学
研究代表者	広瀬茂久東京工業大学, 大学院・生命理工学研究科, 教授 (10134199)
研究分担者	星島一幸東京工業大学, 大学院・生命理工学研究科, 助手加藤明東京工業大学, 大学院・生命理工学研究科, 助手 (40311336) 竹井祥郎東京大学, 海洋研究所, 教授 (10129249)
キーワード	膜7回貫通型受容体 / GPCR / LNB-TM7 / Ig-Hepta / プロセッシング / furin / EGF2 / SEA module
研究概要	Ig-Heptaは膜7回貫通型受容体のサブファミリーLNB-TM7に属する。このサブファミリーに属するメンバーの特徴は,N末端が長く,そこにEGFやカドヘリンやレクチンなどのドメイン構造が繰り返し見られることである。私たちが見出したIg-Heptaの場合は,免疫グロブリン様リピートを有する。私たちは,これまでの研究で,Ig-Heptaが3種のプロテアーゼ(Signal peptidase,未同定のSEA module切断酵素,未同定のGPS切断酵素)によって,以下のように4つのフラグメントに切断されることをアフィニティー精製が可能なIg-Hepta-Fc融合タンパク質のプロセッシング過程を解析することによって明らかにした:(1)シグナルペプチド(1--24),(2)プロEGF2 (25--223),(3)Ig-リピート(224--993),(4)膜7回貫通部(994--1349)。本年度は,プロEGF2領域がFurinによるプロセッシングを受けてEGF2 (52--223)になるという興味深いプロセッシングが起こることを見い出した(この研究には,プロセッシング酵素Furinを欠くLoVo細胞の使用が鍵となった)。このEGF2フラグメントは,負電荷に富む細胞外マトリックスタンパク質に結合しやすいことも明らかになった。この性質は,FGF (fibroblast growth factor)やHGF (hepatocyte growth factor)やHBEGF (heparin binding epidermal growth factor)などと似ている。Ig-Heptaは多数のプロセッシングサイトを有し,ホルモン様分子の前駆体ともなるという事実は,Ig-Heptaの組織分布(腎臓のA型間在細胞や肺)とともに機能を推定する上で有用と思われる。

研究成果

(23件)

すべてその他

すべて文献書誌 (23件)

[文献書誌] 広瀬茂久, 金子豊二: "恐山ウグイの酸性適応機構"エネルギー・資源. 24. 221-225 (2003)
[文献書誌] Hirata, T. et al.: "Mechanism of acid adaptation of a fish living in a pH 3.5 lake."Am.J.Physiol.Regulatory Integrative Comp.Physiol.. 284. R1199-R1212 (2003)
[文献書誌] Hirose, S., et al.: "Molecular biology of major components of chloride cells."Comparative Biochemistry and Physiology Part B. 136. 593-620 (2003)
[文献書誌] Honda, S., Hirose, S.: "Stage-specific enhanced expression of mitochondrial fusion and fission factors during spermatogenesis in rat testis."Biochem.Biophys.Res Commun.. 311. 424-432 (2003)
[文献書誌] Ishizuka, M., et al.: "Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis."Biochem.Biophys.Res Commun.. 301. 1079-1085 (2003)
[文献書誌] Mistry, A.C., et al.: "FHL5, a novel member of the FHL family, localizes to actin stress fiber of the pillar cells and shows volume-dependent enhanced."Mol.Biol.Cell. 14. 240a (2003)
[文献書誌] Suzuki, Y., et al.: "Expression of the K^+ channel Kir7.1 in the developing rat kidney : role in K^+ excretion."Kidney Int.. 63. 969-975 (2003)
[文献書誌] Hyodo S., et al.: "A facilitative urea transporter is localized in the renal collecting tubule of the dogfish Triakis scyllia."J Exp Biol.. 207(Pt 2). 347-356 (2004)
[文献書誌] Inoue K, Takei Y., et al.: "Asian medaka fishes offer new models for studying mechanisms of seawater adaptation."Comp Biochem Physiol [B]. 136(4). 635-645 (2003)
[文献書誌] Takei Y, et al.: "Identification of novel adrenomedullin in mammals : a potent cardiovascular and renal regulator."FEBS Lett.. 556(1-3). 53-58 (2004)
[文献書誌] Ogoshi M., et al.: "Identification of a novel adrenomedullin gene family in teleost fish."Biochem Biophys Res Commun.. 311(4). 1072-1077 (2003)
[文献書誌] Li YY, Inoue K, Takei Y.: "Interrenal steroid 21-hydroxylase in eels : primary structure, progesterone-specific activity and enhanced expression by ACTH."J Mol Endocrinol.. 31(2). 327-340 (2003)
[文献書誌] Kawakoshi A, et al.: "A single and novel natriuretic peptide is expressed in the heart and brain of the most primitive vertebrate, the hagfish (Eptatretus burgeri)."J Mol Endocrinol.1. 31(1). 209-220 (2003)
[文献書誌] Inoue K, et al.: "Four functionally distinct C-type natriuretic peptides found in fish reveal evolutionary history of the natriuretic peptide."Proc Natl Acad Sci U S A.. 100(17). 10079-10084 (2003)
[文献書誌] Yuge S, Inoue, et al.: "A novel guanylin family (guanylin, uroguanylin, and renoguanylin) in eels : possible osmoregulatory hormones in intestine and kidney."J Biol Chem.. 278(25). 22726-22733 (2003)
[文献書誌] Li YY, Inoue K, Takei Y.: "Steroidogenic acute regulatory protein in eels : cDNA cloning and effects of ACTH and seawater transfer on its mRNA expression."Zoolog Sci.. 20(2). 211-219 (2003)
[文献書誌] Inoue K, et al.: "Growth hormone and insulin-like growth factor I of a euryhaline fish Cottus kazika : cDNA cloning and expression after seawater."Gen Comp Endocrinol.. 131(1). 77-84 (2003)
[文献書誌] Li YY, Takei Y., et al.: "Ambient salinity-dependent effects of homologous natriuretic peptides (ANP, VNP, and CNP) on plasma cortisol level in the eel."Gen Comp Endocrinol. 130(3). 317-323 (2003)
[文献書誌] Inoue K., et al.: "C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : primary structure and vasorelaxant activities."Gen Comp Endocrinol.. 130(2). 185-192 (2003)
[文献書誌] Miyazaki H., et al.: "Kidney-specific chloride channel, OmClC-K, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney."Proc Natl Acad Sci U S A.. 99(24). 15782-15787 (2002)
[文献書誌] Eckert SM, et al.: "Effects of angiotensin II and natriuretic peptides of the eel on prolactin and growth hormone release in the tilapia, Oreochromis mossambicus."Gen Comp Endocrinol.2. 130(3). 333-339 (2002)
[文献書誌] Balment RJ., et al.: "Isolation, synthesis, and biological activity of flounder [Asn1,Ile5,Thr9] angiotensin I."Gen Comp Endocrinol.. 130(1). 92-98 (2003)
[文献書誌] Goering LM, et al.: "An interacting network of T-box genes directs gene expression and fate in the zebrafish mesoderm."Proc Natl Acad Sci U S A.. 100(16). 9410-9415 (2003)

2003 年度 実績報告書

塩類細胞の分子解剖と分化誘導機構

研究代表者

広瀬 茂久 東京工業大学, 大学院・生命理工学研究科, 教授 (10134199)

研究成果

[文献書誌] 広瀬茂久, 金子豊二: "恐山ウグイの酸性適応機構"エネルギー・資源. 24. 221-225 (2003)

[文献書誌] Hirata, T. et al.: "Mechanism of acid adaptation of a fish living in a pH 3.5 lake."Am.J.Physiol.Regulatory Integrative Comp.Physiol.. 284. R1199-R1212 (2003)

[文献書誌] Hirose, S., et al.: "Molecular biology of major components of chloride cells."Comparative Biochemistry and Physiology Part B. 136. 593-620 (2003)

[文献書誌] Honda, S., Hirose, S.: "Stage-specific enhanced expression of mitochondrial fusion and fission factors during spermatogenesis in rat testis."Biochem.Biophys.Res Commun.. 311. 424-432 (2003)

[文献書誌] Ishizuka, M., et al.: "Molecular cloning and characteristics of a novel zinc finger protein and its splice variant whose transcripts are expressed during spermatogenesis."Biochem.Biophys.Res Commun.. 301. 1079-1085 (2003)

[文献書誌] Mistry, A.C., et al.: "FHL5, a novel member of the FHL family, localizes to actin stress fiber of the pillar cells and shows volume-dependent enhanced."Mol.Biol.Cell. 14. 240a (2003)

[文献書誌] Suzuki, Y., et al.: "Expression of the K^+ channel Kir7.1 in the developing rat kidney : role in K^+ excretion."Kidney Int.. 63. 969-975 (2003)

[文献書誌] Hyodo S., et al.: "A facilitative urea transporter is localized in the renal collecting tubule of the dogfish Triakis scyllia."J Exp Biol.. 207(Pt 2). 347-356 (2004)

[文献書誌] Inoue K, Takei Y., et al.: "Asian medaka fishes offer new models for studying mechanisms of seawater adaptation."Comp Biochem Physiol [B]. 136(4). 635-645 (2003)

[文献書誌] Takei Y, et al.: "Identification of novel adrenomedullin in mammals : a potent cardiovascular and renal regulator."FEBS Lett.. 556(1-3). 53-58 (2004)

[文献書誌] Ogoshi M., et al.: "Identification of a novel adrenomedullin gene family in teleost fish."Biochem Biophys Res Commun.. 311(4). 1072-1077 (2003)

[文献書誌] Li YY, Inoue K, Takei Y.: "Interrenal steroid 21-hydroxylase in eels : primary structure, progesterone-specific activity and enhanced expression by ACTH."J Mol Endocrinol.. 31(2). 327-340 (2003)

[文献書誌] Kawakoshi A, et al.: "A single and novel natriuretic peptide is expressed in the heart and brain of the most primitive vertebrate, the hagfish (Eptatretus burgeri)."J Mol Endocrinol.1. 31(1). 209-220 (2003)

[文献書誌] Inoue K, et al.: "Four functionally distinct C-type natriuretic peptides found in fish reveal evolutionary history of the natriuretic peptide."Proc Natl Acad Sci U S A.. 100(17). 10079-10084 (2003)

[文献書誌] Yuge S, Inoue, et al.: "A novel guanylin family (guanylin, uroguanylin, and renoguanylin) in eels : possible osmoregulatory hormones in intestine and kidney."J Biol Chem.. 278(25). 22726-22733 (2003)

[文献書誌] Li YY, Inoue K, Takei Y.: "Steroidogenic acute regulatory protein in eels : cDNA cloning and effects of ACTH and seawater transfer on its mRNA expression."Zoolog Sci.. 20(2). 211-219 (2003)

[文献書誌] Inoue K, et al.: "Growth hormone and insulin-like growth factor I of a euryhaline fish Cottus kazika : cDNA cloning and expression after seawater."Gen Comp Endocrinol.. 131(1). 77-84 (2003)

[文献書誌] Li YY, Takei Y., et al.: "Ambient salinity-dependent effects of homologous natriuretic peptides (ANP, VNP, and CNP) on plasma cortisol level in the eel."Gen Comp Endocrinol. 130(3). 317-323 (2003)

[文献書誌] Inoue K., et al.: "C-type natriuretic peptide of rainbow trout (Oncorhynchus mykiss) : primary structure and vasorelaxant activities."Gen Comp Endocrinol.. 130(2). 185-192 (2003)

[文献書誌] Miyazaki H., et al.: "Kidney-specific chloride channel, OmClC-K, predominantly expressed in the diluting segment of freshwater-adapted tilapia kidney."Proc Natl Acad Sci U S A.. 99(24). 15782-15787 (2002)

[文献書誌] Eckert SM, et al.: "Effects of angiotensin II and natriuretic peptides of the eel on prolactin and growth hormone release in the tilapia, Oreochromis mossambicus."Gen Comp Endocrinol.2. 130(3). 333-339 (2002)

[文献書誌] Balment RJ., et al.: "Isolation, synthesis, and biological activity of flounder [Asn1,Ile5,Thr9] angiotensin I."Gen Comp Endocrinol.. 130(1). 92-98 (2003)

[文献書誌] Goering LM, et al.: "An interacting network of T-box genes directs gene expression and fate in the zebrafish mesoderm."Proc Natl Acad Sci U S A.. 100(16). 9410-9415 (2003)

2003 年度実績報告書

広瀬茂久東京工業大学, 大学院・生命理工学研究科, 教授 (10134199)