| 研究課題/領域番号 |
14F04762
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| 研究機関 | 筑波大学 |
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研究代表者 |
ラザルス ミハエル 筑波大学, 国際統合睡眠医科学研究機構, 准教授 (80469650)
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| 研究分担者 |
MCEOWN KRISTOPHER 筑波大学, 国際統合睡眠医科学研究機構, 外国人特別研究員
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| 研究期間 (年度) |
2014-04-25 – 2017-03-31
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| キーワード | sleep / apetite |
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| 研究実績の概要 |
We developed a novel mechanical device with the intention of producing sleep loss in mice over a 72-hour period. The SRD was a wire-mesh-grid device placed in the bottom of the housing cage. EEG/EMG recordings indicated that the SRD significantly reduced rapid-eye movement (REM) sleep during the dark period at 24-48-hours (p<.05) and 48-72-hours after device introduction. In addition, the SRD increased wakefulness during the dark period 48-72-hours after device introduction (p<.05). We then tested the effect of the SRD on stress and fear responses in mice. We found that 72-hours of SRD exposure significantly increased plasma corticosterone levels compared to naïve control mice (p<.01). However, mice exposed to the SRD had significantly lower plasma corticosterone levels compared to caffeine injected mice (30 mg/kg, i.p; p<.05). Finally, SRD exposure did not effect unconditioned fear behavior in the elevated plus maze (all comparisons p>.05). These results suggest that SRD exposure produces mild stress responses, however, unconditioned fear is unaffected by SRD exposure.
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| 現在までの達成度 (区分) |
現在までの達成度 (区分)
1: 当初の計画以上に進展している
理由
While we are in the process of finalizing data collection, we have started to write a paper, entitled "Medial prefrontal cortex neuronal inhibition reverses the effects of rapid eye movement sleep loss on highly palatable food consumption in mice."
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| 今後の研究の推進方策 |
We will explore the interacting roles of the PFC, amygdala and NAc in modulating sleep and appetite for highly palatable foods. The IVM-GluClαβ technique will be used to specifically inhibit neuronal functioning in the NAc shell, amygdala and PFC. Sleep architecture and appetite for highly palatable foods will be measured while inactivating these brain regions. After EEG recording and food consumption measures are obtained, mice will be sacrificed and the location of AAV microinjections within the brain will be visualized using high resolution light/fluorescence microscopy via identification of fluorescent mCherry protein.
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