2003 年度実績報告書

海洋動物におけるミトコンドリアゲノミクスの展開

研究課題

研究課題/領域番号	15380131
研究種目	基盤研究(B)
研究機関	東京大学
研究代表者	西田睦東京大学, 海洋研究所, 教授 (90136896)
研究分担者	宮正樹千葉県立中央博物館, 動物学研究科, 上席研究員 (30250137)
キーワード	海洋動物 / 魚類 / 甲殻類 / ミトコンドリアDNA / mtDNA / ミトコンドリアゲノミクス / 分子系統 / 種査定
研究概要	人為的影響が巨大かつ複雑になる中で、生物多様性を維持しつつ水産生物資源の持続的利用を図る必要性はますます高くなっている。水圏資源生物の集団構造の把握は困難なことが多く、また卵や幼生の種識別はきわめて困難で、これらのことが合理的な資源管理方策を策定する上での妨げとなっている。こうした状況の改善のために、集団や種の信頼の高いDNA標識が強く求められている。本研究は、DNA標識源として重要なミトコンドリア(mt) DNAを取り上げ、これまで実施して来た魚類mtDNA全塩基配列分析研究をさらに推し進めるとともに、研究対象を甲殻類にまで広げ、海洋動物のミトコンドリアゲノミクスの新たな展開を目指すものである。 1年目の今年度は、これまで入手できていなかった魚類および甲殻類の標本収集に努め、約200種の貴重な標本試料を得た。魚類標本の種査定はわれわれ自身でおこない、甲殼類標本の種査定は甲殻類分類学の専門家である千葉中央博物館の駒井智幸氏の協力を得ておこなった。魚類約100種および甲殻類約10種について、当研究グループで確立した効率的なmtDNA全塩基配列決定法を活用して、mtDNA全塩基配列を決定した。甲殻類ではとくに重要な資源生物を含む十脚類(エビ・カニ類)に力点を置いて研究を進めた。得られたミトコンドリアゲノムデータは、すでに集積したデータと照らし合わせながら、その特徴や変異性について検討した。さらに、データ解析のための計算環境を整備した上で、いくつかの分類群に焦点を絞って分子系統解析をおこなった。得られた解析結果に総合的な考察を加え、論文化を進めた。

研究成果

(26件)

すべてその他

すべて文献書誌 (26件)

[文献書誌] Yamanoue, Yusuke: "Mitochondrial genomes and phylogeny of the ocean sunfishes (Tetraodontiformes : Molidae)."Ichthyological Research. (in press). (2004)
[文献書誌] Mabuchi, Kohji: "Gene rearrangements and evolution of tRNA pseudogenes in the mitochondrial DNA of the parrotfishes (Teleostei : Perciformes : Scaridae)."Journal of Molecular Evolution. (in press). (2004)
[文献書誌] Mabuchi, Kohji: "Molecular phylogeny of the antitropical genus Pseudolabrus (Perciformes : Labridae) : evidence for a Southern Hemisphere origin."Molecular Phylogenetics and Evolution. (in press). (2004)
[文献書誌] Kumazawa, Yoshinori: "Tempo of mitochondrial gene evolution : Can mitochondrial DNA be used to date old divergence?"Proceedings ICES 8. (in press). (2004)
[文献書誌] Mabuchi, Kohji: "Genetic differentiations between two color-morphs of Apogon taeniophorus from Southern Japan."Ichthyological Research. (in press). (2004)
[文献書誌] Inoue, Jun G.: "Mitogenomic evidence for the monophyly of elopomorph fishes (Teleostei) and the evolutionary origin of the leptocephalus larva."Molecular Phylogenetics and Evolution. (in press). (2004)
[文献書誌] Ikejima, Kou: "Molecular phylogeny and possible scenario of ponyfish (Perciformes : Leiognathidae) evolution."Molecular Phylogenetics and Evolution. (in press). (2004)
[文献書誌] Inoue, Jun, G.: "Evolution of the deep-sea gulper eel mitochondrial genomes : large-scale gene rearrangements originated within the eels."Molecular Biology and Evolution. (in press). (2004)
[文献書誌] Mukai, Takahiko: "Genetic differentiation of the brackish water goby, Eutaeniichthys gilli (Perciformes, Gobiidae), between the Japanese and the Ryukyu Archipelagos."Biogeography. 5. 49-53 (2003)
[文献書誌] Mabuchi, Kohji: "Genetic comparison of two color-morphs of Apogon properuptus from southern Japan."Ichthyological Research. 50. 293-296 (2003)
[文献書誌] Iguchi, Kei'ichiro: "Morphological and genetic analysis of fish of a Carassius complex (Cyprinidae) in Lake Kasumigaura with reference to the taxonomic status of two all-female triploid morphs."Biological Journal of Linnean Society. 79. 351-357 (2003)
[文献書誌] 向井貴彦: "日本産ドンコにおけるミトコンドリアDNAの系統と関東地方への人為移植の分子的証拠"魚類学雑誌. 50. 71-76 (2003)
[文献書誌] Yamazaki, Yuji: "Mitochondrial DNA sequence divergence between two cryptic species of genus Lethenteron, with reference to an improved identification technique."Journal of Fish Biology. 62. 591-619 (2003)
[文献書誌] Watanabe, Katsutoshi: "Genetic population structure of Japanese bagrid catfishes."Ichthyological Research. 50. 140-148 (2003)
[文献書誌] Ishiguro, Naoya: "Basal euteleostean relationships : A mitogenomic perspective on the phylogenetic reality of the "Protacanthopterygii"."Molecular Phylogenetics and Evolution. 27. 476-488 (2003)
[文献書誌] Saitoh, Kenji: "Mitochondrial genomics of ostariphysan fishes : perspectives on phylogeny and biogeography."Journal of Molecular Evolution. 56. 464-472 (2003)
[文献書誌] Watanabe, Katsutoshi: "Genetic relationships and origin of the two geographic populations of the fishwater threespine stickleback, 'Hariyo'"Zoological Science. 20. 265-274 (2003)
[文献書誌] Inoue, Jun G.: "Basal actinopterygian relationships : a mitogenomic perspective on the phylogeny of the "ancient fish""Molecular Phylogenetics and Evolution. 26. 110-120 (2003)
[文献書誌] Miya, Masaki: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences."Molecular Phylogenetics and Evolution. 26. 121-138 (2003)
[文献書誌] Machida, Ryuji J.: "Large-scale gene rearrangements in the mitochondrial genomes of two calanoid copepods Eucalanus bungii and neocalanus cristatus (Crustacea), with notes on new versatile primers for the _<sr>RNA and COI genes."Gene. (in press). (2004)
[文献書誌] Yamauchi, Mitsugu M.: "A PCR-based approach for sequencing the mitochondrial genomes of decapod crusta-ceans, with a practical example from the kuruma prawm Marsupenaeus japonicus."Marine Biotechnology. (in press). (2004)
[文献書誌] Machida, Ryuji J.: "Organization of the mitochondrial genome of Antarctic krill, Euphausia superba (Crustacea : Malacostraca)."Marine Biotechnology. (in press). (2004)
[文献書誌] Nohara, Masahiro: "Vipoosit Manthacitra, and Teruaki Nishikawa. Ancient phylogenetic separation between Pacific and Atlantic cephalochordates as revealed by mitochondrial genome analysis."Zoological Science. 21. 203-210 (2004)
[文献書誌] Yamauchi, Mitsugu M.: "Complete mitochondrial DNA sequence in swimming crab, Portunus trituberculatus (Crustacea ; Decapoda : Brachyura)."Gene. 311. 129-135 (2003)
[文献書誌] Watanabe, Toshiki: "Polymorphism in the nucleotide sequence of a mitochondrial intergenic region in the scleractinian coral Galaxea fascicularis."Marine Biotechnology. (in press). (2004)
[文献書誌] 前川光司(編著): "サケ・マスの生態と進化"文一総合出版, 東京(印刷中). (2004)

2003 年度 実績報告書

海洋動物におけるミトコンドリアゲノミクスの展開

研究代表者

西田 睦 東京大学, 海洋研究所, 教授 (90136896)

研究成果

[文献書誌] Yamanoue, Yusuke: "Mitochondrial genomes and phylogeny of the ocean sunfishes (Tetraodontiformes : Molidae)."Ichthyological Research. (in press). (2004)

[文献書誌] Mabuchi, Kohji: "Gene rearrangements and evolution of tRNA pseudogenes in the mitochondrial DNA of the parrotfishes (Teleostei : Perciformes : Scaridae)."Journal of Molecular Evolution. (in press). (2004)

[文献書誌] Mabuchi, Kohji: "Molecular phylogeny of the antitropical genus Pseudolabrus (Perciformes : Labridae) : evidence for a Southern Hemisphere origin."Molecular Phylogenetics and Evolution. (in press). (2004)

[文献書誌] Kumazawa, Yoshinori: "Tempo of mitochondrial gene evolution : Can mitochondrial DNA be used to date old divergence?"Proceedings ICES 8. (in press). (2004)

[文献書誌] Mabuchi, Kohji: "Genetic differentiations between two color-morphs of Apogon taeniophorus from Southern Japan."Ichthyological Research. (in press). (2004)

[文献書誌] Inoue, Jun G.: "Mitogenomic evidence for the monophyly of elopomorph fishes (Teleostei) and the evolutionary origin of the leptocephalus larva."Molecular Phylogenetics and Evolution. (in press). (2004)

[文献書誌] Ikejima, Kou: "Molecular phylogeny and possible scenario of ponyfish (Perciformes : Leiognathidae) evolution."Molecular Phylogenetics and Evolution. (in press). (2004)

[文献書誌] Inoue, Jun, G.: "Evolution of the deep-sea gulper eel mitochondrial genomes : large-scale gene rearrangements originated within the eels."Molecular Biology and Evolution. (in press). (2004)

[文献書誌] Mukai, Takahiko: "Genetic differentiation of the brackish water goby, Eutaeniichthys gilli (Perciformes, Gobiidae), between the Japanese and the Ryukyu Archipelagos."Biogeography. 5. 49-53 (2003)

[文献書誌] Mabuchi, Kohji: "Genetic comparison of two color-morphs of Apogon properuptus from southern Japan."Ichthyological Research. 50. 293-296 (2003)

[文献書誌] Iguchi, Kei'ichiro: "Morphological and genetic analysis of fish of a Carassius complex (Cyprinidae) in Lake Kasumigaura with reference to the taxonomic status of two all-female triploid morphs."Biological Journal of Linnean Society. 79. 351-357 (2003)

[文献書誌] 向井貴彦: "日本産ドンコにおけるミトコンドリアDNAの系統と関東地方への人為移植の分子的証拠"魚類学雑誌. 50. 71-76 (2003)

[文献書誌] Yamazaki, Yuji: "Mitochondrial DNA sequence divergence between two cryptic species of genus Lethenteron, with reference to an improved identification technique."Journal of Fish Biology. 62. 591-619 (2003)

[文献書誌] Watanabe, Katsutoshi: "Genetic population structure of Japanese bagrid catfishes."Ichthyological Research. 50. 140-148 (2003)

[文献書誌] Ishiguro, Naoya: "Basal euteleostean relationships : A mitogenomic perspective on the phylogenetic reality of the "Protacanthopterygii"."Molecular Phylogenetics and Evolution. 27. 476-488 (2003)

[文献書誌] Saitoh, Kenji: "Mitochondrial genomics of ostariphysan fishes : perspectives on phylogeny and biogeography."Journal of Molecular Evolution. 56. 464-472 (2003)

[文献書誌] Watanabe, Katsutoshi: "Genetic relationships and origin of the two geographic populations of the fishwater threespine stickleback, 'Hariyo'"Zoological Science. 20. 265-274 (2003)

[文献書誌] Inoue, Jun G.: "Basal actinopterygian relationships : a mitogenomic perspective on the phylogeny of the "ancient fish""Molecular Phylogenetics and Evolution. 26. 110-120 (2003)

[文献書誌] Miya, Masaki: "Major patterns of higher teleostean phylogenies : a new perspective based on 100 complete mitochondrial DNA sequences."Molecular Phylogenetics and Evolution. 26. 121-138 (2003)

[文献書誌] Machida, Ryuji J.: "Large-scale gene rearrangements in the mitochondrial genomes of two calanoid copepods Eucalanus bungii and neocalanus cristatus (Crustacea), with notes on new versatile primers for the _<sr>RNA and COI genes."Gene. (in press). (2004)

[文献書誌] Yamauchi, Mitsugu M.: "A PCR-based approach for sequencing the mitochondrial genomes of decapod crusta-ceans, with a practical example from the kuruma prawm Marsupenaeus japonicus."Marine Biotechnology. (in press). (2004)

[文献書誌] Machida, Ryuji J.: "Organization of the mitochondrial genome of Antarctic krill, Euphausia superba (Crustacea : Malacostraca)."Marine Biotechnology. (in press). (2004)

[文献書誌] Nohara, Masahiro: "Vipoosit Manthacitra, and Teruaki Nishikawa. Ancient phylogenetic separation between Pacific and Atlantic cephalochordates as revealed by mitochondrial genome analysis."Zoological Science. 21. 203-210 (2004)

[文献書誌] Yamauchi, Mitsugu M.: "Complete mitochondrial DNA sequence in swimming crab, Portunus trituberculatus (Crustacea ; Decapoda : Brachyura)."Gene. 311. 129-135 (2003)

[文献書誌] Watanabe, Toshiki: "Polymorphism in the nucleotide sequence of a mitochondrial intergenic region in the scleractinian coral Galaxea fascicularis."Marine Biotechnology. (in press). (2004)

[文献書誌] 前川光司(編著): "サケ・マスの生態と進化"文一総合出版, 東京(印刷中). (2004)

2003 年度実績報告書

西田睦東京大学, 海洋研究所, 教授 (90136896)