| 研究実績の概要 |
In our study of 2015 fiscal year, we carefully analyzed the physiological significance of erythroid and megakaryocytic master transcription factor GATA1 gene silencing in hematopoietic stem cell (HSC). We found that silencer loss of GATA1 locus in HSC exhausts the population of HSC by inducing its apoptosis. Consequently, the GATA1 over-expressed mice show severe anemia due to total HSC exhaustion.
By generating HSC specific and inducible Cre mice lines and crossing these mice with Dnmt1 flow allele, we found that DNMT1 is responsible for Gata1 gene silencing in HSC. Meanwhile, transcription factor GATA2 activates Gata1 gene expression in absence of Gata1 HSC silencer element which recruit DNMT1.
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